Professional Documents
Culture Documents
SCIENCE RESEARCH 4
MAY 2023
ii
CURRICULUM VITAE
Personal Data: Born on November 12,2005, in Batangas City. The third child of
Education:
CURRICULUM VITAE
Personal Data: Born on August 10 2005, in Salawagan, Quezon . The only child of
Mr. Romeo A. Lumapas and Mrs. Hyde D. Lumapas. Currently residing at Prk.
2014-2018
CURRICULUM VITAE
Grade: 11-Euclid
Personal Data: Born on January 10 2006, in Kibawe. Second child of Mr. Danilo C.
Sarausa and Ms. Aeresme S. Sarausa. Currently residing in B10 L26 Grand
Education:
Primary: School of the Morning Star, Syre Highway, Valencia City, Bukidnon,
Valencia City Central School, Syre Highway, Valencia City, Bukidnon, Academic
Year 2013-2014
School of the Morning Star, Syre Highway, Valencia City Bukidnon, Academic Year
2015-2018
CURRICULUM VITAE
Grade: 11 euclid
Personal Data: Born on october 18, 2005, in Adventist Medical Center Valencia
City Bukidnon. Third child of Mr. Oliver Owen L. Garcia, and Mrs. Haidee Helena
UNIVERSITY.
Education:
Primary: School of the Morning Star, Syre Highway, Valencia City, Bukidnon,
ACKNOWLEDGEMENT
The researchers would like to extend their deepest appreciation to the following
individuals who have been of help to them by sharing their time and knowledge,
and had been a guide before, during, and after the course of the study. Above all,
they thank and praise the Lord God Almighty for providing them with the
duration of this research. Sir Sherwin M. Cupida, their science research adviser,
for being the light that guided them through the process of conducting our
research and for providing suggestions, comments, and patience that helped and
inspired them in carrying out their research. Dr. Joemar B. Capuyan, principal of
their school, for his unending support during the study's conduct.To their Science
Research IV, sir. Vanie Y Benben, for the assistance he provided in their research
paper and for making additional suggestions.To their science research panelists,
who evaluated and recommended the research paper and its content. To their
beloved teachers, who provided new knowledge and guidance in carrying out this
research. The researchers are eternally grateful. May God continue to bless you
TABLE OF CONTENTS
TITLE PAGE i
CURRICULUM VITAE ii
ACKNOWLEDGEMENT vi
TABLE OF CONTENTS vii
LIST OF TABLES ix
LIST OF FIGURES x
ABSTRACT xi
INTRODUCTION 1
Background of the study 1
Objective of the study 5
Hypothesis of the study 5
Significance of the study 6
Scope and limitation of the study 7
Operational definition of terms 7
REVIEW OF RELATED LITERATURE 8
METHODOLOGY 17
Research design 17
Locale of the study 18
Material 19
Experimental setup 21
Data gathering 21
Statistical analysis 22
RESULTS AND DISCUSSIONS 23
CONCLUSION AND RECOMMENDATION 27
REFERENCES 29
x
LIST OF TABLES
Table Page
LIST OF FIGURE
Figure Page
ABSTRACT
Water is essential for human life and our day-to-day activities. Its existence
provided us with many benefits for the thousand years of our existence. However,
everyone is experiencing a lack of clean water daily, and our sources are
gradually getting scarce. In this study, the researchers used the most known
filtration system with aloe vera gel and citrus peels (orange peels) . Aloe vera
plant offers a sustainable solution for the removal of various pollutants from
water. There were 3 treatments used on the tainted water. The were three
treatment as follows T0: tainted water, T1: 50 g of aloe vera and 50 g of
powdered citrus peels,T2: 50 g of aloe vera and 100 grams of powdered citrus
peels, T3: 50 grams of aloe vera and 150 grams of powdered citrus peels pH
level and bacterial content were used to measure the effectiveness of the water
purification device used. A comparative study was applied to compare the treated
water to the untreated water collected . The results showed that the treated
water bacterial content had risen. This study was conducted for 2 months with
the remaining time to write and finalize the papers.
Keywords: water, aloe vera gel, citrus peels, water turbidity, pH level, bacterial
content , purification.
Chapter 1
INTRODUCTION
Water is essential for human life and our day-to-day activities. Its existence
provided us with many benefits for the thousand years of our existence. However,
everyone is experiencing a lack of clean water daily, and our sources are gradually
getting scarce. Deteriorating water quality, water scarcity is considered one of the
al 2016). Limited water sources can affect countries that had barely funds to start
water cleaning facilities or lack natural clean water sources that are deep wells or
underground river systems. The lack of clean water was either because of industrial
Water pollution and contamination happen every time whether it's from a normal
wind blowing or even rain, microplastics and harmful microorganisms can be mixed
with a clean and unpolluted water source. The Philippines had been experiencing
flooding and the one who was affected by the natural disasters, areas who had been
affected will be cut off from the clean water supply. Because of these disasters, many
people in our country are experiencing a shortage of clean water. That's why we've
used aloe vera gel and citrus (orange peels) as the main ingredients for our water
wonder plant that is used for antibacterial and antimicrobial (Minwuyelet, Moges
& Gashe, 2017), and this plant was shown to exhibit water purification properties
(Arjunan et al., 2012), and citrus peels (orange peels) is naturally occurring
antioxidants and antibacterial (Gou, Shan, Yang, et al., 2020). Thus, we are doing this
research for the benefit of the people who need it and for additional knowledge about
The Aloe vera plant has been known and used for centuries for its health, beauty,
medicinal and skin care properties. The name Aloe vera derives from the Arabic word
“true.”(Surjushe,Vasani, & Saple, 2008). In filtration and purification, Aloe vera plant
offers a sustainable solution for the removal of various pollutants from water.
phagocytic leukocytes. Such types, it has more antimicrobial value like medicines.
The aim of this study are evaluating antimicrobial activity of aloe vera gel such as
Citrus fruits belong to the family Rutaceae that are grown all over the world
and have numerous therapeutic properties like anticancer, anti-tumor and anti-
present in the citrus fruits and it also have an important role in herbal medicine
(Sanofer, 2014). Antioxidants are molecules that can slow down or prevent
which naturally occur inside the human body, or what can be obtained by
consuming food and other drugs. Citrus (orange, lemon, pomegranate, citrus, etc.)
contains a group of vitamins (C and E), which are the most powerful antioxidants
medicine, citrus fruits are the natural resources in developing new drugs. The
antibacterial activity of natural products from medicinal plants are applicable for
the treatment of bacterial, fungal and viral disease and these are recent technical
advancements taken place in this area. The genus Citrus has a number of species
and hybrids that are well established for their pharmaceutical and economic
degradation of organic pollutants from water (Malik, Ghaffar, & Malik, 2001).
One of the major challenges for many Mediterranean and other countries is
finding viable solutions to tackle water shortage. Some of the major water quality
constraints derive from the high salinity of groundwater and from pollution
4
sources such as: untreated domestic sewage, fertilizers and pesticides from
purification that can help assist readers with their research and applications.
This study investigated the water-purifying potential of aloe vera gel and citrus
using the prototype of water filtration. Specifically, the study aims to:
1.) Determine the physicochemical properties of the produced purified water using the
a. turbidity
b. pH level
2.) Compare the physicochemical properties of the produced purified water with
from the purified water using aloe vera gel and citrus peels and from commercially-
purified water.
from the purified water using aloe vera gel and citrus peels and from commercially-
purified water.
6
people need clean, drinkable water that's needed by many countries. Additionally, this
research could provide additional information and knowledge about another process of
purification. The new process of purification could be used as a substitute for the
purification process today, especially the people who are not supported by the
government and need to travel a few kilometers just to have a few buckets of clean
water.
For the community, using this process of purification can give convenience to the
For the people, by gaining this process, people can clean their water source (if
there is one) that is tainted and cannot be used for their convenience. For the
researchers, we can obtain new ways of water purification by using aloe vera gel and
citrus peels. And by doing so we can learn about what components that aloe vera gel
This study utilized tainted water from a river which will be obtained from the
Pulangi River and its bacterial content that was available in the sample. Water
purification was chosen because it is the most common resource to do. This study will
only use Pulangi River water and it can provide us with knowledge of the water
purification process. However, there will be limitations due to the lack of knowledge
but improvised alternatives shall be observed such as using evaporation of the product
for safety.
Aloe vera is a cactus-like plant that grows in hot, dry climates. This includes their
Citrus is widely cultivated in warm countries for its fruit, which has juicy flesh and a
pulpy rind. Orange peels, which are lignocellulosic biomass-rich materials, have
Filtration is the process in which solid particles in a liquid or gaseous fluid are
removed by the use of a filter medium that permits the fluid to pass through but retains
the solid particles. This includes the removal of nanoparticle silver in the water.
Cotton cloth and flexible polyurethane foam are low-cost relatives to alternate water
filtration techniques.
contaminants, suspended solids, and gasses from water. It is the process of cleaning
Chapter 2
Aloe Vera
Aloe vera plant offers a sustainable solution for the removal of various pollutants
from water. Due to its chemical composition, aloe vera has been explored as a
coagulant/flocculant and biosorbent for water treatment (Katubi et al., 2021). Several
anti-inflammatory properties (Maan et al., 2018). The medicinal value of the plant has
been recognized for centuries because of the gel-like pulp obtained by peeling its
leaves. So, there is a burning need to educate about the importance of Aloe vera for the
human race and popularize it for greater interest (Nandal & Bhardwaj, 2012).
10
burden for society. However, waste peels including orange peels as lignocellulosic
renewable, low cost and sustainable adsorbents for water treatment applications
Water Purification
There are several water purification techniques but adsorption is one of the
simplest, most effective, and economical methods for wastewater purification. In this
polymers; geopolymer cement have been discussed for the removal of different
from wastewater has also been reviewed in this article. Efforts have been made to
reviewed. Attempts have also been made to point out the advantages and drawbacks of
adsorbents and the future research needs in the area of water purification by
Water filtration
Water sources such as lakes, rivers, and groundwater supply much of the water for
domestic use. Some of the water that reaches our household faucets has also been used for
other purposes. Water from these various sources is treated to remove impurities and to make
it suitable for human consumption (Jacobsen, 2004). As the world's population expands, the
need for water is becoming more apparent. Statistics show that freshwater resources are
depleting, causing engineers to think of new ways to create potable water (Gordon, 2013)
12
Anti-bacterial
The ethanol, methanol, and acetone extracts of Aloe vera gel were studied for
using the agar well diffusion method. The extracts showed varied levels of
antimicrobial activity against the tested pathogens. The ethanol and methanol extracts
showed higher activity while the acetone extract showed the least or no activity
against most of the tested pathogens. Fractions obtained from the extracts by Thin
Layer and Column Chromatography were studied for their antagonistic properties
isolated from the ethanol and methanol extracts were identified as p - coumaric acid
(Mol. wt. 165), ascorbic acid (Mol. wt. 177), pyrocatechol (Mol. wt. 110), and
cinnamic acid (Mol. wt. 148), based on Gas Chromatography-Mass Spectrometry. The
study suggests the antimicrobial activity of the A. vera gel extract to be dependent on
the synergistic effect of different compounds. With the broad spectral antimicrobial
effect of aloe vera gel, it could be further recommended in the treatment of various
bacterial diseases.
13
Turbidity
The turbidity of water due to the presence of suspended sediment is measured and
interpreted in a variety of ways, which can lead to the misinterpretation of data. This
paper re-examines the physics of light scattering in water and exposes the extent to
which the reporting of turbidity data is inconsistent (Kitchener et al., 2017). The
performance of different machine learning models was found to be close to each other
and showed similar patterns considering the accuracy and uncertainty of the forecasts.
The results revealed that flow discharge influenced the water salinity and turbidity of
the bay in which the models including the river flow as input variables had better
performance compared with those excluding the flow time series. Among the water
quality parameters investigated in this research, river flow made the most and least
improvement in the efficiency of the models applied for forecasting turbidity and
pH Level
We all know what water is, and we often take it for granted. Because it seems so
natural, we seldom question how we see water. But the spectre0 of a worldwide water
crisis suggests that there might be something fundamentally wrong with the way we
think about water (Linton 2010). Water is needed to be safe too for drinking access,
because too low of a pH can result in increased micronutrient availability that can lead
with excessive levels of iron and manganese can result in iron and/or manganese
measure of how acidic/basic water is. The range goes from 0-14, with 7 being
neutral. pH level of less than 7 indicates acidity, whereas pH level of greater than
7 indicates a base and this data are WHO guidelines for drinking water (Islam,
Conductivity
the purity of the water. The method is based on the measurement of the resistance of a
frequency to find the value at zero inverse frequency. The temperature coefficient of
the conductivity is 2% per Kelvin at 25°C and this limits the uncertainty of the
Bacteria
Bacterial systematics has not yet reached a consensus for defining the
fundamental unit of biological diversity, the species. The past half-century of bacterial
species as phenotypic and genetic clusters, but species demarcation has not been
bacterial preferences may suggest new ways to modulate the expression of virulence
factors and therefore decrease the reliance on antibiotics to fight infection (Galperin
2018).
16
Conceptual Framework
METHODOLOGY
Research Design
the purification of the water from aloe vera gel and citrus, which would be the
observed method for the data collection. Quantitative research deals with quantifying
and analyzing variables to get results. It involved the utilization and analysis of
numerical data using specific statistical techniques to answer questions like who, how
much, what, where, when, how many, and how (Apuke, 2017). Experimental research
Its general goal is to figure out the independent variable's effect on the dependent
variable. Therefore, this study is quantitative since it will be dealing with numerical
data and specific statistical techniques. In addition, it is also experimental where the
effects of the independent variable, Aloe vera gel, and citrus peels, on the dependent
This study would be conducted at Juanilla, Valencia City, Bukidnon, and P-13,
Materials
In this study the materials used are sand for the filtration, rocks to remove the
large particle included in the wastewater, activated charcoal for bacterial removal, aloe
vera gel (crushed) main material for purification, citrus peel (powdered) also a main
material for purification, the strainer is a barrier for materials so that it won't mix, and
a ceramic water filter will filter all the components of the water that is visible in the
eye and for the purified water to be clear in the naked eye. All of these materials were
used in the process of making the prototype water filtration device and the researchers
produced water.
Prototype (Creation)
For making the prototype, the researchers acquired plastic bottles, nails,
wood, nylon, hooks, and metal net (screen). First they cut the water bottles in
halves and used the upper portion for the prototype. Next, they've used the wood
and nails to create a foundation, Using a flat square plywood for the base and
three 2x2 wood to make a triangular pillar to hold the prototype in place. After
making the foundation, the researchers inserted the hooks in the pillars and tied
nylons to the plastic bottles for it to hang in the middle. Lastly, using the metal
As for this study, it should be an experiment and so researchers would use aloe
vera and citrus for this water purifying study this study would have 4 treatments (T0,
Would be replicated 3 times. T0 will serve as the control which would not undergo any
purification process, Negative control for clean or purified water, and positive control
for tainted water. The process would be measured by how much gel and peels would
be added.
T1: 50 grams of aloe vera and 50 grams of powdered citrus peels T2: 50 grams of aloe
vera and 100 grams of powdered citrus peels T3: 50 grams of aloe vera and 150 grams
The researchers traveled to the Pulangi River and collected samples on the
shoreline during fair weather. Water obtained is sealed in a clean water container and
stored in a cool container so the bacterial content will be the same even after traveling
to the research site. The oranges were bought in a roadside stall and the other materials
like the ceramic water filter, activated charcoal, silica sand, and pH paper were bought
online using shopee and lazada respectively. The sand and pebbles are put in the
prototype, the aloe vera and orange peels in the second prototype, while the charcoal
22
and the ceramic water filter are put in the third prototype. The pH level is obtained
Statistical Analysis
differences between variables. This included applying the kruskal-Wallis test to compare the
groups and identify any significant differences in their outcome. By utilizing these statistical
method, By utilizing these statistical methods, We were able to obtain the data needed and
gain a more comprehensive understanding of the relationship between variables in the sample
or population
23
Chapter 4
As we undergo the first conduct to determine the expected outcomes, the filter did
not purify the tested water. Even using the same method from the previous study, the
filter still did not work. The possible reasons might be from the filter structure itself,
the aloe vera and the charcoal turned the water darker making it more difficult to filter,
and the aloe vera gel made the filtration more difficult to process because it blocks the
passage of the water through the ceramic water filter device. The same result happened
in the other treatments, in the first treatment the water turned black also in the second
treatment, the last treatment turned the water into darkish green. This is the result of
the method we followed although some studies use the same method, the method
The ceramic water filtration device failed to purify the contaminated water. The
purified water still contained impurities, including charcoal particles and citrus
particles. We observed that the flow rate was slow, and the device clogged quickly,
reducing the filtration efficiency. The test results showed that the device was
There are a few studies that did the same method and as we observed in the
experiment, we noticed that aloe vera and charcoal helped purify the water. Same in
the other treatments, the two solutions might be the cause of the failed outcomes. The
study of Bhatnagar, A. (2015) is one of the previous studies that followed the same
method of purifying the water, and the study of (Yusuf, Adio-Yusuf, & Obalowu,
24
2019) also did a water filtration prototype with the same idea as the researchers
but only used sand and pebbles for filtration.This may be the reason why the
researchers didn't achieve good results, the researchers did both tasks that are
researchers only had one main goal( filter or purify) rather than having them
both.
pH level
shown below.
T0 6 7
T1 6 7
T2 5 7
T3 5 7
pH is a measure of how acidic/basic water is. The range goes from 0 - 14,
with 7 being neutral. pHs of less than 7 indicate acidity, whereas a pH of greater
and hydroxyl ions in the water. As shown in Table 2, the results given were lower
than 7 and were not acceptable by human consumption given that our results
25
have higher acidity than average water. The data acquired to the purification is
that (T0) and (T1) had the same pH level but (T2) and (T3) has become more
acidic due to having more citrus resulting in lower pH level,but this data is
Bacterial content
The range should not be more than 500 and exceeding that is bad for
exceeded the limit of bacterial content only water can take for it to be acceptable
for human consumption. At (T0) the bacterial content was only 396 which is still
safe because less than 500 bacterial content is safe for human consumption, but
(T1) increased the bacterial content 10x of (T0) causing it to be unsafe for
consumption and making the treatment a failure. This happened because the
prototype made by the researchers was an open system which makes airborne
bacteria to contaminate the water and because the water treatment is very slow
to process, bacterias had a lot of time to contaminate the water. Another problem
26
is that the researchers only had money to test one sample due to having low
funds, resulting in only testing one treatment, which is the reason why the data
failed to purify contaminated water. The results concluded that the device used in the
experiment was not effective in removing impurities from the contaminated water. The
failure of the ceramic water filtration device to purify the contaminated water could be
due to several factors, including the design of the prototype, the quality of the
materials used, the flow rate, and the filtration media used. The porous ceramic
material used in the device was not effective in removing all impurities from the
contaminated water as the ceramic filter had remnants of the past trial still on the filter
that have contributed to the rising bacteria content on the water. The design of the
prototype could have contributed to the failure of the experiment due to the opted open
design we have on our prototype that has led to the bacterial content rising instead of
decreasing. The results of this experiment suggest that this particular ceramic water
filtration device is not an effective means of purifying contaminated water. The failure
of the prototype device might be also due to our inexperienced researchers that while
searching for the design of the prototype, we didn't factor in the effects of the open
design of the prototype and how it could affect the outcome of the experiment and
how drastic it affect the result when the bacterial content was exposed on the
laboratory. We recommend that the prototype be closed because it affects the bacterial
lemon because it was more acidic, and the acidity can affect the bacterial content
In the bacterial content shown in our treatments, it multiplied into ten (10) times
the original amount than it already had. The researchers concluded that this happened
due to our prototype and treatment process used. The prototype happens to be an open
system which causes airborne bacterias to contaminate the treatment, also, the system's
filtration process is very slow which lets the bacteria have a lot of time to contaminate
the treatment. The researchers recommended that the prototype should be fixed and
turned to be a closed system and the treatment process should be done in a closed area
because the last results happens only because the researchers conducted their research
in an open area which can lead bacterias to contaminate it. After this recommendation
is to be finished, This can lessen the bacterial content that the open system has
procured.
29
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APPENDICES
1. Appendix 1:Documentation
Appendix 2: Results
Laboratory results
T0 and T1 in a microscope