CvSU Mission

CvSU Vision Republic of the Philippines

The premier university in
historic Cavite recognized for
excellence in the development
of morally upright and globally
competitive individuals.
CAVITE STATE UNIVERSITY
Bacoor City Campus
Soldiers Hills IV, Molino VI,
City of Bacoor, Cavite
 (046) 476 - 5029
www.cvsu.edu.ph
Cavite State University shall provide
excellent, equitable and relevant educational
opportunities in the arts, science and
technology through quality instruction and
relevant research and development activities.
It shall produce professional, skilled and
morally upright individuals for global
competitiveness.

Name: ____David, John Vincent I._________________ Date: _____5/5/2022_______

Course & Section: ____BSP 1-1_____ Score: ____________

Activity #5

Extract DNA from Banana

I. Objective: To be able to see what does DNA look like

II. Materials:

1 pc. Cold Banana toothpick saucer

½ cup cold water blender cold ethyl alcohol (70-95%)

Pinch of salt coffee filter 3 pcs. clear plastic cups

1 tbsp. liquid detergent rubber band fork

½ tsp. pineapple juice

III. Procedure
1. Peel a piece of cold banana and then mash.
2. Mix mashed banana, water and salt in a blender for 15 secs.at high speed.
3. Cover one plastic cup with a coffee filter.
4. Secure with a rubber band.
5. Pour the banana mixture on the filter.
6. Remove the coffee filter and add the liquid detergent.
7. Mix gently to avoid forming bubbles.
8. Leave it for 10 mins. Slowly pour 1/3 of the mixture on another cup.
9. Add the pineapple juice.
10. Slightly tilt the cup then pour 1/8 cup of ethyl alcohol through the side of the cup.
11. Wait for 5 mins. or until a cloudy substance appears. That substance is the DNA of banana!
12. Scoop it using a toothpick.
1V. Questions:

1.What is the role of salt? Dishwashing liquid?

The dishwashing liquid breaksdown the cell's outer most membrane while the salt helps seperate the dna
from other cells.

2. Why do you use a filter?

To remove large debris that can hinder the cells structural integrity.

3. What does cold alcohol do?

It preserves the DNA. And increase the outcome/yield of it?

4. Can you use room temperature alcohol or warm alcohol? Why or why not?

Colder temps tends to preserve dna more than warmer temp. Extreme warm temperatures can damage
the structural integrity of the dna

5. Can you use other fruits or vegetables as sources of DNA? Explain your answer.

Strawberry is one of the example of a source of dna so the answer is yes. I've watched a scientist extract
a dna of strawberries and tasted it.