TEST TEST FOR RGT./CPDS. USED TEST CPDS.

TEST RESULT

Molisch Test CHOs (Carbs) Molisch rgt. (alpha Glucose, Xylose, Violet ring/layer
napthol in ethanol; Sucrose, Lactose, *all tested positive
conc. H2SO4) Starch

Moore’s Test Reducing Sugar Alkali (NaOH) Glucose, Fructose Caramel formation,
(Rxn. with yellow-dark brown
Alkali) *glucose, fructose

Tollen’s Test Aldehyde Tollen’s rgt. (AgNO3, Glucose Silver mirror

NaOH, NH3) *glucose

Fehling’s Test Aldehyde (no Fehling’s A & B Glucose, Xylose, Brick red ppte
aromatic) Lactose, Sucrose, *glucose, xylose, lactose (+)
Starch, Glycogen *sucrose, starch, glycogen (-)

Benedict’s Test Reducing Sugar Benedict’s rgt. Glucose, Lactose, Brick red ppte
Sucrose, Starch *glucose, lactose (+)
*sucrose, starch (-)

Nylander’s Test Glucose in urine Nylander’s rgt. Glucose, Xylose, Brown-black ppte
Lactose, Sucrose, *glucose, xylose, lactose (+)
Starch Glycogen *sucrose, starch glycogen (-)

Bial’s Orcinol Differentiate Bial’s orcinol rgt. Xylose, Glucose, Pentoses (blue sol.)
Test pentoses (5) from (orcinol, HCl, FeCl3) Fructose *xylose
hexoses (6) Hexoses (muddy brown sol.)
*glucose, fructose

Mucic Acid Test Galactose Conc. HNO3 Galactose, Insol. Mucic acid crystals
Lactose, Glucose, *galactose, lactose (+)
Sucrose *glucose, sucrose (-), no
crystals

Seliwanoff’s Keto Sugar/Ketose Seliwanoff’s rgt. Glucose, Fructose, Cherry red solution
Test Sucrose, Starch *fructose, sucrose (+)
*glucose, starch (-), light
yellow/peach sol.

Osazone Reducing Sugar Glucose, Fructose, Same Osazone: glucose,

Formation (identify monosac) Mannose, fructose, mannose
Galactose, Maltose Formed in 7 mins.: galactose
Formed in 10-15 mins.: maltose

Iodine Test Polysaccharides I2 sol. (orange-brown Starch, Glycogen, Blue sol.: starch (deep blue) (+)
(Starch) sol.) Glucose Orange brown: glycogen
Light yellow/Colorless: glucose

Solubility Test Fats/Oils 5% HCl, 5% NaOH, dami 5% HCl: (In) corn oil, margarine
H2O, Ether 5% NaOH: (In) corn oil,
margarine
H2O: (In) corn oil, margarine
Ether: (S) corn oil, margarine