Professional Documents
Culture Documents
PATHOGENS
PROF: DR. DE OCAMPO
DATE:
PROPERTY OF FMDLP
MODULE 2 LESSON 1: PATHOGENS In a good infection control practice, all surfaces that have a
The dental setting with its personnel has been recognized by the potential risk of contamination should be cleaned regularly and
Center for Disease Control has the greatest risk of exposure in a particularly before and between all patient treatment occasions.
wide variety of microorganism that is present in the blood and
As a healthcare provider, dental workers must understand the
saliva of their patients being treated.
influence of these mode of transmission and strictly implement
safety protocol measures to reduce the spread of infection in the
A pathogen is an organism that causes disease. In order for a clinical area.
microorganism to cause an infection it must attach to the host’s
targeted cells, penetrate the surfaces of the body and establish VIRUS
themselves leading to cell destruction. While the body is well Viruses are made up of a piece of genetic code, such as
protected from microbial invasion by an intact skin, the mucosa DNA or RNA, and protected by a coating of protein. Once
lined orifices of the body are sites for the potential entry of you are infected, viruses invade host cells within your
infection caused by these microorganisms. Depending on the body. They use the components of the host cell to
type, these pathogens can spread and cause infection. replicate, producing more viruses. Viruses are different to
bacteria in that they are strict parasites and rely
TRANSMISSION OF INFECTION IN THE DENTAL OFFICE completely on the host’s cells to multiply and survive
ENVIRONMENT
Infection may be transmitted in the dental operatory by blood or
saliva through direct contact, droplets or aerosol. Patients and
dental health care workers have the potential of transmitting
infections to each other.
Transmission of Microbes in the dental office environment will
depend on:
1. pathogenicity of microorganism
2. ability of the microorganism to survive in the
environment After the replication cycle is complete, the host cell will be
3. route of transmission so full of copied viruses that it will burst open releasing
Transmission of infection in the dental set up is through aerosol large numbers of viruses that will then go on and infect
with the use of dental equipment such as air rotor or mechanical further target cells either in the host or will be shed from
scaler which produces high energy fine spray that can pick up
the host and will go on to infect further individuals. This
microbes from patients’ oral tissue and distribute them in the
environment. process results in widespread destruction of the host
tissues causing the signs and symptoms of the disease.
BACTERIA
Bacteria are microscopic, single-celled organisms that thrive in
Another way in which transmission of pathogenic diverse environments. These organisms can live soil, the ocean
microorganism can occur in the dental office is through the and inside the human gut. Bacteria are
motile and have the ability to ‘swim’ and
equipment or work surfaces known as fomites. Microbes
multiply when in a moist environment.
have poor adherence to surface of materials such as
stainless steel used in dental instruments or to smooth
surface materials. However, if theses surfaces are Sometimes bacteria lend us a helping
contaminated with organic materials such as blood, saliva hand, such as by curdling milk into
yogurt or helping with our digestion. In other cases, bacteria are
or body secretion, adherence will be greatly enhanced.
destructive, causing diseases like pneumonia and methicillin-
Therefore, a delay in cleaning of the contaminated surface resistant Staphylococcus aureus (MRSA) and tuberculosis.
will lead to an increase in the number of bacteria present.
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INFECTION CONTROL MIDTERM 4TH YEAR – 1ST SEMESTER (SY: 2023-2024)
PATHOGENS
PROF: DR. DE OCAMPO
DATE:
PROPERTY OF FMDLP
In the discipline of Dentistry, a bacterial infection causes handling uncooked food or feces.
demineralization and destruction of the hard tissues Causes of Cook food to its recommended internal
Dental Caries enamel, dentin, and cementum) that can lead to temperature.
dental caries. The bacteria most responsible for dental cavities Drink clean water, including bottled water when
are the mutans streptococci, most prominently Streptococcus you’re traveling.
mutans and Streptococcus sobrinus, and lactobacilli. If left
untreated, the disease can lead to pain, tooth loss, and infection. Avoid swallowing water from lakes, streams, or
Today, caries remains one of the most common diseases ponds.
throughout the world. Avoid cat litter and feces when you’re pregnant.
FUNGI
MODULE 2 LESSON2: BLOOD BORNE DISEASES OF
CONCERN TO DENTISTRY
Bloodborne pathogens are
microorganisms such as viruses or
bacteria that are carried in blood and
can cause disease in people.
Bloodborne pathogens such as HBV
and HIV can be transmitted through
contact with infected human blood and other potentially
There are millions of species of fungi on Earth. It can be found infectious body fluids. In Dentistry, these pathogens are
anywhere in the environment, including indoors, and human transmitted from saliva and blood from dental procedures or from
skin. When they overgrow, they cause an infection. accidents arising from a dental procedure.
Fungi cells contain a nucleus protected by a membrane and a HUMAN IMMUNODEFICIENCY VIRUS (HIV)
thick cell wall. Their structure can make them harder to kill. An
undiagnosed fungal disease can lead to serious illness and KEY POINTS
death. A key clue to when a sick person may have a fungal Dental health care personnel should strive to create a
disease is that he or she is being treated with medicine for safe, welcoming and nonjudgmental environment for all
another type of infection but does not get better. patients in order to encourage dental care
Fungal infections, although uncommon in dental practice may Infection with human immunodeficiency virus
indicate a more serious systemic illness. Oral candidiasis is the (HIV) predisposes people to certain oral health
most common fungal infection encountered in dentistry. problems
Patients who are HIV-positive can receive
PARASITES routine dental care.
Dentists and all staff with direct patient contact
Parasites are organisms that should follow Standard Precautions with all
behave like tiny animals. They live patients.
and feed from at the expense of the
host
Human Immunodeficiency Virus, commonly known as HIV, is a
Parasites usually enter the body virus that can eventually become AIDS if left untreated. AIDS,
through the mouth and skin. which stands for acquired immunodeficiency syndrome, will
eventually lead to death. Currently there are no treatments that
Parasites that enter through the mouth are swallowed and can
can completely cure the HIV virus, so once you are infected, you
remain in the intestine or burrow through the intestinal wall and
will have it for life.
invade other organs. Often parasites enter the mouth through
fecal-oral transmission. HIV is classified as a retrovirus and works by targeting the body’s
immune system, specifically CD4 T cells, making it difficult for
Some parasites can enter directly through the skin. Others are
the body to fight off infections. Eventually the body will succumb
transmitted by insect bites.
to opportunistic infections, which leads to death. HIV usually
Once upon a time, dental patients believed a toothache was progresses very slowly, often taking years for a person to exhibit
caused by a tooth worm eating away their tooth from the inside signs and symptoms.
out. Modern-day medical science has proven that there is no
Although HIV cannot be cured at this time, but there are
such parasite and that the concept is merely superstition. But
treatments designed to slow the progression. ART, or
evidences of oral protozoa have been identified and studied in
the antiretroviral therapy has dramatically helped decreased
cases of gingivitis and periodontal diseases.
the number of lives lost with HIV. The goal of ART is reducing
the viral load in a patient’s body, therefore reducing your chance
Infection Control
of transmission to others.
There are several steps you can take to lower your risk of
contracting a parasitic infection:
MODE OF TRANSMISSION
Practice safe sex, using a condom. HIV is transmitted most commonly from anal or vaginal
Wash your hands regularly, especially after sex and through the sharing of needles or syringes
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INFECTION CONTROL MIDTERM 4TH YEAR – 1ST SEMESTER (SY: 2023-2024)
PATHOGENS
PROF: DR. DE OCAMPO
DATE:
PROPERTY OF FMDLP
HEPATITIS VIRUSES
Key Points
Hepatitis A (HAV), hepatitis B virus (HBV), and
hepatitis C virus (HCV) are prevalent and infectious
causes of liver disease.
HEPATITS B (HBV) HBV is transmitted perinatally,
HBV infection is preventable through vaccination,
percutaneously, and through
most commonly administered in 2 or 3 injections over
sexual contact.
the course of 6 months.
It is also spread through open
Although there is no vaccine for HCV, current
cuts and sores through
treatment regimens can cure more than 90% of
individuals in close contact
cases
HBV infection is more
commonly seen in adulthood as
a result of sexual transmission
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INFECTION CONTROL MIDTERM 4TH YEAR – 1ST SEMESTER (SY: 2023-2024)
PATHOGENS
PROF: DR. DE OCAMPO
DATE:
PROPERTY OF FMDLP
and IV drug use. HCV has been detected in saliva, but no undisputed case of
It is not spread through HCV salivary transmission has been documented.11
breastfeeding, food, water,
casual touching, kissing, or Unlike HBV infection, there is no effective vaccine or post-
exposure prophylaxis available for HCV infection. Prior infection
with hepatitis C does not protect against later infection.
INFECTION CONTROL
PATHOGENS
PROF: DR. DE OCAMPO
DATE:
PROPERTY OF FMDLP
active sores.
MODULE 2 LESSON 3: OTHER DISEASES OF CONCERN TO
DENTISTRY In rare circumstances, HSV-1 infection can be transmitted
from a mother with genital HSV-1 infection to her infant
HERPES SIMPLEX 1-3 during delivery to cause neonatal herpes
KEY POINTS:
Relevance to Dentistry
HSV-1 is
mainly HSV can cause persistent infection and is shed in saliva. There
is also evidence of transmission of HSV to health-care workers
from patients and from dental workers to patients. According to
the Center for Disease Control and Prevention (CDC), there are
two treatments you should be rendering: emergency treatment
for dental conditions and treatment of the lesions. No other
treatment should be rendered.
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INFECTION CONTROL MIDTERM 4TH YEAR – 1ST SEMESTER (SY: 2023-2024)
PATHOGENS
PROF: DR. DE OCAMPO
DATE:
PROPERTY OF FMDLP
Infection occurs through inhalation of mycobacteria, Additional guidelines as suggested in the research of
which then travel to the alveoli of the lungs; only people Rajasekar et al are as follows:
with active disease can spread the infection.
1. Limit the use of ultrasonic scalers and highspeed
A person with latent tuberculosis is not infectious; he or
handpieces in actively infected patients. High volume suction
she can be treated in the dental office under standard
is mandatory for carrying out any procedure to minimize
infection control precautions.
aerosol generation.
A person with active tuberculosis, standard precautions
are insufficient to prevent transmission of the bacterium. 2. Use rubber dam isolation with high vacuum suction.
However, if the patient has productive cough, it is better to
avoid Rubber dam.
Tuberculosis is caused by
infection with Mycobacterium 3. Maintenance of proper hand hygiene, personal protective
tuberculosis. Infection occurs equipment’s like eye shields, facemasks, head caps, gloves
through inhalation of, which then and surgical gowns.
travel to the alveoli of the lungs.
4. Standard face masks do not protect against TB
Only people with active disease
transmission, hence particulate face masks should be used
can spread the infection.
and often changed at regular intervals. Face masks should
Tuberculosis is transmitted
have at least 95% Bacterial filtration efficiency (BPE) for
through airborne particles, known
particles 3µm diameter. While treating patients with symptoms
as “droplet nuclei,” which can be
of active TB, the operator should wear respirators rather than
generated when people with
routine face masks.
pulmonary or laryngeal
tuberculosis sneeze, cough, speak or sing. These small particles 5. TB rooms should have effective air evacuation with either
(1 to 5 micrometers in diameter) can stay suspended in the air exhausted or HEPA-filtered if re circulation is necessary.
for hours. If a susceptible person inhales droplet nuclei
containing M. tuberculosis, infection may begin if the organisms 6. Regular fumigation of dental operatories. Cleaning and
reach the alveoli. disinfecting critical and semi critical contact surfaces like
Dental chair and accessories. Anti-bacterial sprays may be
used.
MODE OF TRANSMISSION
7. Use of barrier techniques.
The aerosols that form in the dental clinics from both the
equipment and patient sources can cause droplet infections. 8. Use of high efficacy filters or UV light in the exhaust air
ducts.
Potential routes of transmission of Tuberculosis infection
include: 9. All dental settings should conduct an annual risk
assessment for TB transmission.
a. Direct contact with blood, oral fluids or other body
fluids.
b. Indirect contact with contaminated objects including
instruments, equipment or environmental surfaces.
c. Contact of eyes, nose, mouth and / or mucous
membranes with droplets/splatter containing
microorganisms.
d. Inhalation of Airborne microorganisms that can remain
suspended in the air for longer periods of time.
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INFECTION CONTROL MIDTERM 4TH YEAR – 1ST SEMESTER (SY: 2023-2024)
PATHOGENS
PROF: DR. DE OCAMPO
DATE:
PROPERTY OF FMDLP
THE VIRUS
According to the World Health Organization (WHO),
MODE OF TRANSMISSION
coronaviruses make up a large family of viruses that can infect
birds and mammals, including humans. This coronavirus which
sports a spiky projections on their outer surface which resembles The virus can spread from an infected person’s
the points of a crown have been responsible for several mouth or nose in small liquid particles when they
outbreaks around the cough, sneeze, speak, sing or breathe heavily.
world, including the These liquid particles are different sizes, ranging
severe acute respiratory from larger ‘respiratory droplets’ to smaller ‘aerosols.
syndrome (SARS) Other people can catch COVID-19 when the virus
pandemic of 2002-2003 gets into their mouth, nose or eyes, which is more
and the Middle East likely to happen when people are in direct or close
respiratory syndrome contact (less than 1 meter apart) with an infected
(MERS) outbreak in person.
South Korea in 2015. Current evidence suggests that the main way the
virus spreads is by respiratory droplets among
PATHOGENICITY
people who are in close contact with each other.
According to Jan Caretta, an associate professor of microbiology
and immunology at the Stanford University School of Medicine, a
coronavirus's pronged exterior lies a round core shrouded in
proteins and a "greasy" membrane. The core contains genetic Aerosol transmission through aerosol generating
material that the virus can inject into vulnerable cells to infect procedures can occur in specific settings, particularly in
them. So-called spike proteins extend from within the core to the indoor, crowded and inadequately ventilated spaces, where
viral surface and allow the virus to "recognize and latch onto". infected person(s) spend long periods of time with others,
When the spike engages its receptor [on a host cell], a cascade such as restaurants, choir practices, fitness classes,
is triggered, resulting in the merger of the virus with the cell. This nightclubs, offices and/or places of worship.
merger allows the virus to release its genetic material and hijack
the cell's internal machinery. Once this happens, the virus sheds The virus can also spread after infected people sneeze, cough
its coat and turns the cell into a factory that starts churning out on, or touch surfaces, or objects, such as tables, doorknobs
new viruses. and handrails. Other people may become infected by touching
these contaminated surfaces, then touching their eyes, noses
SIGNS AND SYMPTOMS or mouths without having cleaned their hands first.
People with COVID-19 have had a wide range of symptoms
ranging from mild symptoms to severe illness. Symptoms may
COVID 19 AND DENTISTRY
appear 2-14 days after exposure to the virus. People with
these symptoms may have COVID-19:
Dentistry is one of the most exposed and affected profession the
Fever or chills Covid 19 contagion has brought. During the height of pandemic,
Cough dental procedures across the world were halted as dental
Shortness of breath or difficulty breathing professionals are identified being at high risk for nosocomial
Fatigue infection and can become potential carriers of the disease.
Muscle or body aches Posted in several studies, the risks can be attributed to the
unique nature of dental interventions, which include aerosol
Headache generation, handling of sharps, and proximity of the provider to
New loss of taste or smell the patient’s oropharyngeal region. The dental office has also
Sore throat the potential to expose patients to cross contamination if not well
Congestion or runny nose equipped with infection control measures and dental health
Nausea or vomiting professionals not knowledgeable in the safety precautions.
Tele screening and triaging is the initial measure of precaution to
Diarrhea
assess the urgency of the illnesses and injuries prior to
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INFECTION CONTROL MIDTERM 4TH YEAR – 1ST SEMESTER (SY: 2023-2024)
PATHOGENS
PROF: DR. DE OCAMPO
DATE:
PROPERTY OF FMDLP
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