AGRIC CS 311: FRUITS AND PLANTATION CROP PRODUCTION

Laboratory Activity
DATE ACTIVITY MODE OF REMARKS
ASSESSMENT
Week (10- Production of seedling Physical counting
17) 1. 10 Mulberry (cuttings)
2. 10 any citrus plants
(seedlings)
3. 10 Mango (seedlings)
1. 5 Approach grafting Number of survive
2. 5 Simple layering

AGRIC CS 311 (FRUITS AND PLANTATION)

LABORATORY PLAN

Job sheet no. 2

Project Title: Asexual Propagation using Approach grafting and Simple Layering
Performance Objectives 1. To apply and demonstrate the use of this special techniques in
asexual propagation
2. Observe the plant reaction as undergo to asexual propagation
Materials Grafting knife, grafting tape, sanitary tissue or alcohol, shovel
Steps and Procedure 1. Prepare the needed materials in executing the activity.
Sanitation must be observed while conducting the activity to
ensure greater chance of survival.
2. Proper selection of the orientation
3. Steps and Procedure of each technique. Please refer to Tech. 1
&2.
4. After observable survival of the clonal. Proceed in potting and
put up in slightly shaded area for acclimatization.
5. Determine the survival rate.
6. Capture video and documentation for consolidation
Assessment method Physical counting of the survive clonal.
***Disappointment is given but practice makes perfect

Worktext in Agri 111- Introduction to Agriculture

MARCEL B. TOMAS & MARY CRIS T. UNDAJON
Compiler
 AGRIC CS 311: FRUITS AND PLANTATION CROP PRODUCTION
Laboratory Activity
Technique 1: Approach Grafting
A plant graft made by joining stock and scion laterally at an intermediate point but leaving
both rooted and uncut until firm union is established when the stock is cut above and the scion
below the union
Steps and Procedure

The approach grafting procedure is as follows:

1. Plant an adapted, growing plant as close to

the base of the non-adapted variety as
possible without extensively damaging the
root structure of the established plant.
2. From both plants closely position shoots
which are at least three-eighths inch diameter
and preferably close to the same size. At the
point where the union is to occur, a slice of
bark one to two inches long is peeled from
both stems. The peeled area should be the same size on each.
3. The two peeled surfaces are then bound tightly together with budding or electrical tape.
Wrap completely with two complete covers around the area where the two peeled areas
are in contact.
4. Remove some of the top portion of the foliage from the adapted variety six to eight
inches above the graft union. This will encourage a more rapid healing of the grafted
union.
5. The union should be complete in four weeks. This type of grafting is most successful if
performed during growth season.
6. After the parts are well united (4 weeks or more),the remainder of the top of the adapted,
native variety can be cut off immediately above the graft union and the bottom or root
system of the non-adapted, yellowing plant can be cut off immediately below the graft
union.
7. The graft union is now completed and the problems of iron chlorosis and indigenous soil
pathogens have been solved if the proper rootstock has been used. Immediately after the
portion of each plant is removed it may be necessary to reduce the leaf area of the top if
wilting occurs because of lack of sufficient root system support. This situation will soon
stabilize. If the only problem has been micronutrient (iron chlorosis) deficiency, the top,
unadapted variety will not need to be detached from its own root system--the approach
grafted, adapted variety root system will "feed" the sickly plant what it needs. However,
if the purpose of the graft is to control soil borne diseases, the susceptible variety should
be detached from its root system and become totally dependent on the root system of the
adapted variety.

https://aggie-horticulture.tamu.edu/propagation/approachgraft/approach.html

Watch the linked here for more info. https://www.youtube.com/watch?v=9ohsVtRtKQ0

Worktext in Agri 111- Introduction to Agriculture
MARCEL B. TOMAS & MARY CRIS T. UNDAJON
Compiler
 AGRIC CS 311: FRUITS AND PLANTATION CROP PRODUCTION
Laboratory Activity
Technique 2: Simple Layering

Simple layering – Simple layering is done by bending a

stem until the middle touches the soil. Push the center of
the stem underground and hold it in place with a U-shaped
pin. Roots will form along the part of the stem that’s
underground.

Steps and Procedure

1. Prepare the materials needed
Serpentine layering – Serpentine layering works for long, flexible branches. Push a portion of the
stem underground and pin it. Weave the stem above the soil, then back down again. This method
gives you two plants instead of just one.

For more info. Watch video here: https://www.youtube.com/watch?v=Q-HaV5IQ-o8

Read more at Gardening Know How: What Is Plant Layering: Learn About Plant Propagation By
Layering https://www.gardeningknowhow.com/garden-how-to/propagation/layering/
propagation-by-layering.htm

Worktext in Agri 111- Introduction to Agriculture

MARCEL B. TOMAS & MARY CRIS T. UNDAJON
Compiler