33.2.

06
AOAC Official Method 947.05
Acidity of Milk
Titrimetric Method
First Action 1947
Final Action
Measure or weigh test portion (ca 20 mL or 20 g) into suitable dish
and dilute with twice its volume CO2-free H2O. Add 2 mL
phenolphthalein and titrate with 0.1M NaOH to first persistent pink.
If measured volume was used, determine its weight from specific
gravity of test sample. Report acidity as % lactic acid by weight. (1
mL 0.1M NaOH = 0.0090 g lactic acid.) If Babcock milk pipet,
989.04C(b) (see 33.2.27), is used, mL 0.1M NaOH required ÷ 20 =
% acid as lactic acid.
Results may also be expressed as mL 0.1M NaOH/100 g test
portion.
References: JAOAC 30, 130(1947); 34, 239(1951).

 2005 AOAC INTERNATIONAL