33.2.

06
AOAC Official Method 947.05
Acidity of Milk
Titrimetric Method
First Action 1947
Final Action

Measure or weigh suitable amount (ca 20 mL or 20 g) sample into

suitable dish and dilute with twice its volume CO2-free H2O. Add
2 mL phenolphthalein and titrate with 0.1M NaOH to first persistent
pink. If measured volume sample was used, determine its weight
from specific gravity of sample. Report acidity as % lactic acid by
weight. (1 mL 0.1M NaOH = 0.0090 g lactic acid.) If Babcock milk
pipet, 989.04B(b) (see 33.2.27), is used, mL 0.1M NaOH required ÷
20 = % acid as lactic acid.
Results may also be expressed as mL 0.1M NaOH/100 g sample.
References: JAOAC 30, 130(1947); 34, 239(1951).

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