Professional Documents
Culture Documents
Microbiology
1. Which of the following bacterial groups is characterized by a thick peptidoglycan layer in their cell walls,
leading to a purple color in the Gram stain?
a) Gram-negative bacteria
b) Gram-positive bacteria
c) Archaea
d) Fungi
Answer: b) Gram-positive bacteria
4. What is the primary role of toxins produced by some Gram-positive bacteria during infection?
a) Promote host cell growth
b) Enhance antibiotic effectiveness
c) Damage host cells and tissues
d) Increase oxygen levels in tissues
Answer: c) Damage host cells and tissues
5. Which laboratory technique is used to differentiate Gram-positive from Gram-negative bacteria based on
cell wall structure?
a) DNA sequencing
b) Electron microscopy
c) Gram staining
d) Western blotting
Answer: c) Gram staining
6. In a patient with a suspected Gram-positive bacterial infection, which laboratory finding might indicate an
inflammatory response?
a) Elevated white blood cell count
b) Decreased red blood cell count
c) Low platelet count
d) Reduced serum glucose levels
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Answer: a) Elevated white blood cell count
7. What type of laboratory test is performed to determine which antibiotics are effective against a specific
Gram-positive bacterial strain?
a) Blood culture
b) Antibiotic susceptibility testing
c) Serological assay
d) PCR analysis
Answer: b) Antibiotic susceptibility testing
8. Which laboratory test can detect specific toxins produced by Gram-positive bacteria in certain infections?
a) Gram staining
b) Blood culture
c) Toxin detection assay
d) Urinalysis
Answer: c) Toxin detection assay
10. Streptococcus pyogenes, also known as Group A Streptococcus, can cause which of the following
conditions?
a) Influenza
b) Stomach ulcers
c) Streptococcal pharyngitis (strep throat)
d) Meningitis
Answer: c) Streptococcal pharyngitis (strep throat)
11. In Gram staining, what color do Gram-positive bacteria appear after the staining process?
a) Purple or blue
b) Red or pink
c) Green
d) Yellow
Answer: a) Purple or blue
12. Which type of Gram-positive bacteria is known for its rod-shaped appearance?
a) Staphylococci
b) Streptococci
c) Bacilli
d) Cocci
Answer: c) Bacilli
13. What is the primary purpose of antibiotic susceptibility testing in the context of Gram-positive bacterial
infections?
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a) To diagnose the infection
b) To determine the bacterial species
c) To assess the patient’s immune response
d) To identify effective antibiotics for treatment
Answer: d) To identify effective antibiotics for treatment
14. Which laboratory test is used to confirm the presence of antibodies produced by the host in response to a
Gram-positive bacterial infection?
a) Blood culture
b) PCR analysis
c) Serological assay
d) Western blotting
Answer: c) Serological assay
15. Which of the following is NOT a common sign of a Clostridium difficile infection?
a) Severe diarrhea
b) Abdominal pain
c) Headache
d) Fever
Answer: c) Headache
16. Which bacterial genus is known for its spherical (round) cell shape and often forms chains or clusters?
a) Staphylococcus
b) Streptococcus
c) Escherichia
d) Salmonella
Answer: b) Streptococcus
17. What type of laboratory test can detect specific bacterial DNA in a clinical sample, aiding in the diagnosis
of a Gram-positive bacterial infection?
a) Blood culture
b) PCR analysis
c) Gram staining
d) ELISA assay
Answer: b) PCR analysis
18. Which Gram-positive bacterial species is commonly associated with food poisoning and can produce toxins
that cause gastrointestinal symptoms?
a) Clostridium difficile
b) Listeria monocytogenes
c) Mycobacterium tuberculosis
d) Escherichia coli
Answer: b) Listeria monocytogenes
19. In a patient with a Streptococcus pneumoniae infection, which respiratory symptom is most commonly
observed?
a) Runny nose
b) Cough and chest pain
c) Nausea and vomiting
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d) Joint pain
Answer: b) Cough and chest pain
20. What laboratory finding is typically associated with an elevated immune response to a Gram-positive
bacterial infection?
a) Decreased white blood cell count
b) Low levels of C-reactive protein (CRP)
c) Elevated erythrocyte sedimentation rate (ESR)
d) Decreased antibody production
Answer: c) Elevated erythrocyte sedimentation rate (ESR)
21. Which of the following bacterial genera includes species responsible for skin infections, such as impetigo
and cellulitis?
a) Clostridium
b) Listeria
c) Staphylococcus
d) Mycobacterium
Answer: c) Staphylococcus
22. Which Gram-positive bacterial species is commonly associated with urinary tract infections (UTIs) and is
known for its ability to form biofilms on catheters?
a) Streptococcus pyogenes
b) Clostridium difficile
c) Enterococcus faecalis
d) Mycobacterium tuberculosis
Answer: c) Enterococcus faecalis
23. In Gram staining, what color do Gram-negative bacteria appear after the staining process?
a) Purple or blue
b) Red or pink
c) Green
d) Yellow
Answer: b) Red or pink
24. Which of the following is a potential complication of untreated Streptococcus pyogenes infections, such as
streptococcal pharyngitis (strep throat)?
a) Kidney failure
b) Hearing loss
c) Blindness
d) Osteoporosis
Answer: a) Kidney failure
25. What is the primary mechanism by which Streptococcus pneumoniae causes pneumonia in the lungs?
a) Toxin production
b) Invasion of lung cells
c) Disruption of mucus production
d) Formation of biofilms
Answer: b) Invasion of lung cells
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26. Which of the following laboratory tests is used to identify specific antibodies against Gram-positive
bacterial antigens in a patient’s blood sample?
a) Blood culture
b) PCR analysis
c) Serological assay
d) Western blotting
Answer: c) Serological assay
27. Which Gram-positive bacterium is known for its role in causing dental caries (cavities) by forming biofilms
on tooth surfaces?
a) Clostridium difficile
b) Streptococcus mutans
c) Staphylococcus aureus
d) Mycobacterium leprae
Answer: b) Streptococcus mutans
28. Which laboratory technique is used to observe the morphology and arrangement of bacterial cells after
Gram staining?
a) Blood culture
b) Electron microscopy
c) ELISA assay
d) Western blotting
Answer: b) Electron microscopy
30. Which laboratory test is often performed to confirm the presence of Gram-positive cocci in clusters in a
clinical sample?
a) PCR analysis
b) Gram staining
c) Blood culture
d) ELISA assay
Answer: b) Gram staining
31. Which Gram-positive bacterium is responsible for causing tuberculosis, a disease that primarily affects the
lungs?
a) Staphylococcus aureus
b) Streptococcus pneumoniae
c) Mycobacterium tuberculosis
d) Clostridium difficile
Answer: c) Mycobacterium tuberculosis
32. Which of the following signs and symptoms is often associated with a Streptococcus pyogenes (Group A
Streptococcus) skin infection?
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a) Frequent urination
b) Nausea and vomiting
c) Fever and sore throat
d) Joint pain and stiffness
Answer: d) Joint pain and stiffness
33. Which laboratory finding may indicate an inflammatory response to a Gram-positive bacterial infection
when measured in a blood test?
a) Elevated white blood cell count
b) Decreased erythrocyte sedimentation rate (ESR)
c) Low levels of C-reactive protein (CRP)
d) Reduced platelet count
Answer: a) Elevated white blood cell count
34. What is the primary function of antibiotic susceptibility testing in the context of Gram-positive bacterial
infections?
a) To diagnose the infection
b) To identify the bacterial species
c) To determine antibiotic resistance
d) To assess patient immunity
Answer: c) To determine antibiotic resistance
35. Which Gram-positive bacterium is commonly associated with foodborne illnesses and can produce heat-
resistant endospores?
a) Listeria monocytogenes
b) Streptococcus pyogenes
c) Clostridium difficile
d) Staphylococcus aureus
Answer: a) Listeria monocytogenes
36. What laboratory technique is used to detect specific bacterial DNA in a clinical sample, allowing for the
diagnosis of a Gram-positive bacterial infection?
a) Blood culture
b) PCR analysis
c) Serological assay
d) Gram staining
Answer: b) PCR analysis
37. Which of the following Gram-positive bacteria is commonly found in the gastrointestinal tract and can
cause urinary tract infections when it enters the urinary system?
a) Clostridium difficile
b) Enterococcus faecalis
c) Staphylococcus aureus
d) Streptococcus pneumoniae
Answer: b) Enterococcus faecalis
38. In Gram staining, what component of the bacterial cell wall retains the crystal violet stain in Gram-positive
bacteria?
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a) Lipopolysaccharide
b) Peptidoglycan
c) Outer membrane
d) Capsule
Answer: b) Peptidoglycan
39. What is the primary purpose of serological assays in diagnosing Gram-positive bacterial infections?
a) To identify bacterial species
b) To assess antibiotic susceptibility
c) To detect specific antibodies in patient serum
d) To visualize bacterial morphology
Answer: c) To detect specific antibodies in patient serum
40. Which of the following is NOT a common clinical manifestation of a Staphylococcus aureus infection?
a) Skin abscess
b) Cellulitis
c) Pneumonia
d) Frequent urination
Answer: d) Frequent urination
a) Virus
b) Bacteria
c) Fungus
a) Streptococcus pneumoniae
b) Escherichia coli
c) Mycobacterium tuberculosis
d) Salmonella enterica
3. Which virus is responsible for causing the common cold, a major respiratory infection?
a) Influenza virus
b) Rhinovirus
c) Human papillomavirus
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d) Hepatitis B virus
a) Plasmodium falciparum
b) Vibrio cholerae
c) Staphylococcus aureus
d) Candida albicans
5. Which of the following bacteria is a major pathogen responsible for food poisoning?
a) Listeria monocytogenes
b) Clostridium botulinum
c) Campylobacter jejuni
d) Streptococcus pyogenes
6. Which major pathogen is associated with causing the sexually transmitted infection known as syphilis?
a) Neisseria gonorrhoeae
b) Treponema pallidum
c) Chlamydia trachomatis
d) Haemophilus influenzae
a) Candida albicans
b) Aspergillus fumigatus
c) Trichophyton rubrum
d) Cryptococcus neoformans
8. What is the major pathogen associated with the development of dental caries (cavities)?
a) Streptococcus mutans
b) Clostridium difficile
c) Pseudomonas aeruginosa
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9. Which virus is considered a major pathogen responsible for causing acquired immunodeficiency
syndrome (AIDS)?
b) Human papillomavirus
d) Epstein-Barr virus
10. Which major pathogen is responsible for causing the majority of urinary tract infections (UTIs)?
a) Escherichia coli
b) Salmonella enterica
c) Staphylococcus aureus
11. Which major pathogen is commonly associated with pneumonia in both adults and children?
a) Streptococcus pyogenes
b) Haemophilus influenzae
c) Pseudomonas aeruginosa
d) Legionella pneumophila
12. What is the major pathogen responsible for causing peptic ulcers in the stomach and duodenum?
a) Streptococcus pneumoniae
b) Helicobacter pylori
c) Escherichia coli
d) Clostridium difficile
13. Which virus is a major pathogen associated with causing cervical cancer?
b) Influenza virus
c) Rotavirus
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14. What is the major pathogen responsible for causing strep throat?
a) Staphylococcus aureus
b) Streptococcus pneumoniae
c) Streptococcus pyogenes
15. Which major pathogen is commonly associated with nosocomial (hospital-acquired) infections,
including urinary tract infections and bloodstream infections?
a) Klebsiella pneumoniae
b) Clostridium difficile
c) Neisseria gonorrhoeae
16. What is the major pathogen responsible for causing the majority of cases of bacterial meningitis in
infants and young children?
a) Listeria monocytogenes
b) Neisseria meningitidis
c) Streptococcus pneumoniae
17. Which major pathogen is associated with causing dental infections and abscesses?
a) Staphylococcus aureus
b) Clostridium botulinum
c) Actinomyces species
18. What is the major pathogen responsible for causing the sexually transmitted infection known as
gonorrhea?
a) Treponema pallidum
b) Neisseria gonorrhoeae
c) Chlamydia trachomatis
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19. Which major pathogen is associated with causing bacterial food poisoning through the production of a
toxin in contaminated food?
a) Salmonella enterica
b) Vibrio cholerae
c) Clostridium botulinum
d) Staphylococcus aureus
20. What is the major pathogen responsible for causing the majority of cases of dental cavities in humans?
a) Candida albicans
b) Streptococcus mutans
c) Escherichia coli
21. Which type of bacteria requires oxygen for growth and metabolism?
a) Anaerobic bacteria
b) Aerobic bacteria
c) Facultative anaerobes
22. What type of bacteria thrives in the presence of oxygen and utilizes it for respiration?
a) Anaerobic bacteria
b) Microaerophilic bacteria
c) Aerobic bacteria
23. Which of the following bacteria is an example of an aerobic pathogen that causes tuberculosis?
a) Clostridium difficile
b) Mycobacterium tuberculosis
c) Escherichia coli
24. Which aerobic bacterium is commonly found in soil and is used for the production of antibiotics like
streptomycin?
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a) Staphylococcus aureus
b) Pseudomonas aeruginosa
c) Mycobacterium leprae
25. What is a well-known aerobic bacterium involved in the decomposition of organic matter in the
environment?
a) Clostridium perfringens
b) Bacillus anthracis
c) Escherichia coli
26. Which aerobic bacterium is commonly associated with urinary tract infections?
a) Staphylococcus epidermidis
b) Proteus mirabilis
c) Clostridium difficile
27. Which aerobic bacterium is responsible for causing whooping cough (pertussis)?
a) Bordetella pertussis
b) Clostridium tetani
c) Salmonella enterica
28. Which aerobic bacterium is commonly associated with respiratory infections and pneumonia in
immunocompromised individuals?
a) Clostridium botulinum
b) Mycoplasma pneumoniae
c) Streptococcus mutans
29. What aerobic bacterium is often found in water and can cause infections in wounds or via ingestion of
contaminated water?
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a) Legionella pneumophila
b) Clostridium difficile
c) Listeria monocytogenes
30. Which aerobic bacterium is associated with the production of greenish-blue pigment and is found in
burn wounds?
a) Staphylococcus aureus
b) Escherichia coli
c) Pseudomonas aeruginosa
31. What aerobic bacterium is commonly used in the dairy industry to produce yogurt and cheese?
a) Lactobacillus acidophilus
b) Clostridium botulinum
c) Mycobacterium tuberculosis
32. Which aerobic bacterium is responsible for causing infections in the respiratory tract, particularly in
individuals with cystic fibrosis?
a) Mycobacterium leprae
b) Streptococcus pneumoniae
c) Pseudomonas aeruginosa
33. Which aerobic bacterium is associated with dental plaque formation and gum disease?
a) Clostridium perfringens
b) Streptococcus mutans
c) Listeria monocytogenes
34. What aerobic bacterium is known for its involvement in causing stomach ulcers?
a) Helicobacter pylori
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b) Clostridium difficile
c) Salmonella enterica
35. Which aerobic bacterium is a common inhabitant of the human skin and is associated with skin
infections?
a) Staphylococcus epidermidis
b) Streptococcus pyogenes
c) Clostridium tetani
36. What is the primary aerobic bacterium responsible for causing Lyme disease?
a) Treponema pallidum
b) Borrelia burgdorferi
c) Chlamydia trachomatis
37. Which aerobic bacterium is a common cause of urinary tract infections in women?
a) Staphylococcus aureus
b) Clostridium difficile
c) Escherichia coli
38. What is a notable aerobic bacterium used in biotechnology for genetic engineering and producing
insulin?
a) Clostridium botulinum
b) Escherichia coli
c) Mycobacterium tuberculosis
39. Which aerobic bacterium is commonly found in the intestines of animals and can cause foodborne
illnesses in humans?
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a) Clostridium perfringens
b) Salmonella enterica
c) Listeria monocytogenes
40. What is the major aerobic bacterium responsible for causing bacterial vaginosis in women?
a) Gardnerella vaginalis
b) Clostridium difficile
c) Streptococcus pneumoniae
Method of disinfection
1. What is the primary goal of sterilization?
a) To reduce the number of microorganisms to a safe level
b) To eliminate all forms of microbial life, including spores
c) To disinfect surfaces and equipment
d) To prevent the growth of bacteria
2. Which method of sterilization involves the use of high-pressure steam to kill microorganisms?
a) Autoclaving
b) Filtration
c) Boiling
d) Pasteurization
3. What is the primary mode of action of ethylene oxide (ETO) as a sterilizing agent?
a) High-temperature steam
b) Alkylating cellular components
c) Oxidizing cellular proteins
d) Filtration of microorganisms
4. Which of the following is a chemical disinfectant commonly used for disinfection of surfaces in
healthcare settings?
a) Filtration
b) Boiling
c) Hydrogen peroxide
d) Autoclaving
5. What is the primary advantage of using ultraviolet (UV) radiation for disinfection?
a) It is effective in killing bacterial spores
b) It is non-toxic to humans
c) It requires high temperatures
d) It is ineffective against viruses
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6. Which method of disinfection involves the removal of microorganisms by passing a liquid or gas
through a filter with tiny pores?
a) Autoclaving
b) Pasteurization
c) Filtration
d) Ethylene oxide sterilization
7. What is the purpose of using chemical disinfectants like chlorine and iodine?
a) To eliminate all forms of microbial life
b) To reduce the number of microorganisms to a safe level
c) To remove dirt and debris from surfaces
d) To enhance the growth of beneficial microorganisms
8. Which method of disinfection is commonly used for disinfecting drinking water and swimming pool
water?
a) Filtration
b) Ultraviolet (UV) radiation
c) Chlorination
d) Autoclaving
9. Which of the following methods of sterilization is NOT suitable for heat-sensitive materials?
a) Autoclaving
b) Dry heat sterilization
c) Ethylene oxide (ETO) sterilization
d) Gamma radiation
10. What is the primary advantage of using gamma radiation for sterilization?
a) It is inexpensive
b) It can be used for heat-sensitive materials
c) It requires minimal exposure time
d) It leaves a residue on sterilized items
11. Which chemical disinfectant is often used for skin antisepsis before medical procedures?
a) Iodine
b) Bleach (sodium hypochlorite)
c) Hydrogen peroxide
d) Ethanol
13. Which of the following is a limitation of using UV-C radiation for disinfection?
a) It is effective against bacterial spores
b) It requires minimal contact time
c) It is ineffective in air purification
d) It does not penetrate surfaces or materials
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14. What is the primary purpose of pasteurization in the food industry?
a) To sterilize food products
b) To extend the shelf life of food
c) To improve the taste of food
d) To remove all microorganisms from food
15. Which sterilization method relies on the use of high-frequency sound waves to disrupt and kill
microorganisms?
a) Autoclaving
b) Ultrasonication
c) Filtration
d) Dry heat sterilization
17. Which of the following is a drawback of using ethylene oxide (ETO) for sterilization?
a) It is highly flammable
b) It leaves a residue on sterilized items
c) It requires high temperatures
d) It is ineffective against bacteria Answer: a) It is highly flammable
18. Which method of disinfection involves the use of chemicals or detergents to remove dirt and organic
material from surfaces before applying a disinfectant?
a) Dry heat sterilization
b) Precleaning
c) Ethylene oxide (ETO) sterilization
d) Gamma radiation Answer: b) Precleaning
19. What is the primary limitation of using dry heat sterilization compared to moist heat methods like
autoclaving?
a) It requires longer exposure times
b) It cannot penetrate materials effectively
c) It is less energy-efficient
d) It is not suitable for heat-sensitive materials Answer: b) It cannot penetrate materials effectively
20. Which disinfection method is often used for decontaminating laboratory equipment like pipettes and
glassware?
a) Autoclaving
b) Pasteurization
c) Chemical disinfection
d) Ultraviolet (UV) radiation Answer: c) Chemical disinfection
21. Which method of disinfection primarily relies on the use of high temperatures to kill or inactivate
microorganisms?
a) Chemical disinfection
b) Physical disinfection
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c) Ultraviolet (UV) radiation
d) Filtration Answer: b) Physical disinfection
22. Which of the following is NOT a commonly used chemical disinfectant in microbiology?
a) Chlorine
b) Ethanol
c) Hydrogen peroxide
d) Glassware Answer: d) Glassware
23. What is the primary mechanism of action of chemical disinfectants like chlorine and iodine?
a) Mechanical removal of microorganisms
b) Altering the pH of the environment
c) Disrupting the cell membrane or proteins
d) Providing nutrients for microorganisms Answer: c) Disrupting the cell membrane or proteins
24. Which of the following is a method of disinfection commonly used in water treatment to kill or
inactivate microorganisms?
a) Autoclaving
b) Pasteurization
c) Filtration
d) Incineration Answer: b) Pasteurization
Bacterial Vaccine
1. What is the primary purpose of a bacterial vaccine?
A. To treat bacterial infections
B. To prevent bacterial infections
C. To boost the immune system
D. To cure viral diseases
3. Which bacterial vaccine type typically provides long-lasting immunity with a single dose?
A. Live attenuated vaccine
B. Inactivated (killed) vaccine
C. Toxoid vaccine
D. Subunit vaccine
4. Which bacterial vaccine type involves using only specific components of the bacterial
cell, such as proteins or polysaccharides?
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A. Live attenuated vaccine
B. Inactivated (killed) vaccine
C. Toxoid vaccine
D. Subunit vaccine
6. Which bacterial vaccine type is safer for individuals with weakened immune systems?
A. Live attenuated vaccine
B. Inactivated (killed) vaccine
C. Toxoid vaccine
D. Subunit vaccine
7. Which bacterial vaccine is used to prevent diseases like diphtheria and tetanus?
A. Live attenuated vaccine
B. Inactivated (killed) vaccine
C. Toxoid vaccine
D. Subunit vaccine
8. Which bacterial vaccine type can provide protection against multiple strains or serotypes
of the same bacterium?
A. Live attenuated vaccine
B. Inactivated (killed) vaccine
C. Toxoid vaccine
D. Subunit vaccine
9. Which bacterial vaccine type often requires booster shots to maintain immunity over
time?
A. Live attenuated vaccine
B. Inactivated (killed) vaccine
C. Toxoid vaccine
D. Subunit vaccine
10. What is the main advantage of bacterial vaccines over antibiotics in controlling bacterial
infections?
A. Bacterial vaccines work faster
B. Bacterial vaccines are more effective
C. Bacterial vaccines prevent infections
D. Bacterial vaccines have fewer side effects
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12. What is the primary function of an adjuvant in a bacterial vaccine?
A. To weaken the bacteria
B. To enhance the vaccine’s shelf life
C. To stimulate the immune response
D. To replace the need for boosters
13. Which bacterial vaccine is commonly administered to travelers going to regions with a
high risk of exposure to Vibrio cholerae?
A. Typhoid vaccine
B. Shigella vaccine
C. Cholera vaccine
D. Salmonella vaccine
14. Which bacterial vaccine type is used to prevent infections caused by Neisseria
meningitidis?
A. Live attenuated vaccine
B. Inactivated (killed) vaccine
C. Toxoid vaccine
D. Conjugate vaccine
16. Which bacterial vaccine is used to prevent diseases like whooping cough and
pertussis?
A. Tuberculosis vaccine
B. Tetanus toxoid vaccine
C. Diphtheria-pertussis-tetanus (DPT) vaccine
D. Haemophilus influenzae type b (Hib) vaccine
19. What is the primary target of the human papillomavirus (HPV) vaccine?
A. Bacterial infections
B. Viral infections
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C. Fungal infections
D. Parasitic infections
20. Which bacterial vaccine type is made from bacterial cell fragments, such as the cell
wall?
A. Live attenuated vaccine
B. Inactivated (killed) vaccine
C. Toxoid vaccine
D. Subunit vaccine Answer: D. Subunit vaccine
22. Which bacterial vaccine is used to prevent infections caused by Clostridium tetani?
A. Pertussis vaccine
B. Tuberculosis vaccine
C. Typhoid vaccine
D. Tetanus toxoid vaccine
23. Which bacterial vaccine type is used to prevent infections caused by Haemophilus
influenzae type b (Hib)?
A. Conjugate vaccine
B. Inactivated (killed) vaccine
C. Toxoid vaccine
D. Subunit vaccine
24. Which bacterial vaccine is given to protect against bacterial pneumonia and ear
infections in young children?
A. Influenza vaccine
B. Pneumococcal conjugate vaccine (PCV)
C. Hepatitis B vaccine
D. Diphtheria-pertussis-tetanus (DPT) vaccine Answer: B. Pneumococcal conjugate
vaccine (PCV)
25. Which bacterial vaccine is used to prevent diseases caused by the bacterium
Bordetella pertussis?
A. Tuberculosis vaccine
B. Tetanus toxoid vaccine
C. Pertussis vaccine
D. Meningococcal vaccine Answer: C. Pertussis vaccine
26. Which bacterial vaccine is given to prevent infections caused by Salmonella typhi, the
bacterium responsible for typhoid fever?
A. Typhoid vaccine
B. Cholera vaccine
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C. Diphtheria-pertussis-tetanus (DPT) vaccine
D. Tuberculosis vaccine Answer: A. Typhoid vaccine
27. Which bacterial vaccine type contains a weakened but live version of the bacterium?
A. Inactivated (killed) vaccine
B. Conjugate vaccine
C. Live attenuated vaccine
D. Subunit vaccine Answer: C. Live attenuated vaccine
28. Which bacterial vaccine is used to prevent infections caused by the bacterium that can
lead to cervical cancer?
A. Tuberculosis vaccine
B. Tetanus toxoid vaccine
C. Hepatitis B vaccine
D. HPV vaccine Answer: D. HPV vaccine
29. Which bacterial vaccine is used to prevent diseases like pneumonia, meningitis, and
septicemia caused by Streptococcus pneumoniae?
A. Pneumococcal conjugate vaccine (PCV)
B. Tetanus toxoid vaccine
C. Tuberculosis vaccine
D. Pertussis vaccine Answer: A. Pneumococcal conjugate vaccine (PCV)
30. Which bacterial vaccine is administered to prevent infections caused by the bacterium
responsible for causing anthrax?
A. Anthrax vaccine
B. Tuberculosis vaccine
C. Meningococcal vaccine
D. Cholera vaccine Answer: A. Anthrax vaccine
3. What term describes the ability of bacteria to survive exposure to an antibiotic that would normally kill
them?
a) Antibiotic susceptibility
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b) Antibiotic tolerance
c) Antibiotic resistance
d) Antibiotic persistence
Answer: c) Antibiotic resistance
4. Which of the following is NOT a common way that bacteria acquire antibiotic-resistance genes?
a) Mutation
b) Horizontal gene transfer
c) Transformation
d) Vertical gene transfer
Answer: d) Vertical gene transfer
6. Which type of antibiotic is most susceptible to resistance development due to its narrow spectrum of
activity?
a) Broad-spectrum antibiotics
b) Penicillins
c) Macrolides
d) Fluoroquinolones
Answer: b) Penicillins
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b) Developing new antibiotics more slowly
c) Using combination therapy with multiple antibiotics
d) Discontinuing the use of antibiotics altogether
Answer: c) Using combination therapy with multiple antibiotics
11. Which of the following is an example of a gram-negative bacterium known for its antibiotic resistance?
a) Staphylococcus aureus
b) Escherichia coli (E. coli)
c) Streptococcus pyogenes
d) Clostridium difficile
Answer: b) Escherichia coli (E. coli)
12. What is the primary reason behind the overuse and misuse of antibiotics?
a) To promote the development of antibiotic resistance
b) To reduce the cost of healthcare
c) To prevent bacterial infections
d) To speed up the recovery from viral infections
Answer: d) To speed up the recovery from viral infections
13. What is the term for the phenomenon where bacteria that were once susceptible to an antibiotic
become resistant to it over time?
a) Antibiotic mutation
b) Antibiotic insensitivity
c) Antibiotic switching
d) Antibiotic adaptation
Answer: d) Antibiotic adaptation
14. Which of the following factors contributes to the rapid spread of antibiotic resistance in healthcare
settings?
a) Strict infection control measures
b) Rapid development of new antibiotics
c) Frequent patient transfers between healthcare facilities
d) Limited use of antibiotics
Answer: c) Frequent patient transfers between healthcare facilities
15. What is the name of the enzyme produced by some bacteria that inactivates beta-lactam antibiotics like
penicillin?
a) Ribonuclease
b) Beta-lactamase
c) DNA polymerase
d) RNA ligase
Answer: b) Beta-lactamase
16. Which of the following is NOT a common mechanism of antibiotic resistance in bacteria?
a) Efflux pumps
b) Cell wall synthesis inhibition
c) Target site modification
d) Enhanced antibiotic uptake
Answer: d) Enhanced antibiotic uptake
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17. What is the term for the process by which antibiotic-resistant genes can be transferred from one
bacterial species to another through direct contact or exchange of genetic material?
a) Vertical gene transfer
b) Horizontal gene transfer
c) Gene inversion
d) Gene duplication
Answer: b) Horizontal gene transfer
20. Which class of antibiotics is known for its potential to cause Clostridium difficile infection, a common
healthcare-associated infection?
a) Tetracyclines
b) Macrolides
c) Fluoroquinolones
d) Aminoglycosides
Answer: c) Fluoroquinolones
21. What is the primary mode of action of antibiotics that inhibit bacterial protein synthesis, such as
tetracyclines?
a) Disrupting cell membrane integrity
b) Inhibiting DNA replication
c) Inhibiting RNA synthesis
d) Blocking ribosome function
Answer: d) Blocking ribosome function
23. Which of the following is an example of an antibiotic-resistant “superbug” that has caused significant
healthcare challenges?
a) Streptococcus pneumoniae
b) Mycobacterium tuberculosis
c) Methicillin-resistant Staphylococcus aureus (MRSA)
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d) Salmonella enterica
Answer: c) Methicillin-resistant Staphylococcus aureus (MRSA)
24. What is the term for the phenomenon where antibiotics that are no longer effective against a bacterial
infection must be replaced by more potent antibiotics?
a) Antibiotic escalation
b) Antibiotic cycling
c) Antibiotic rotation
d) Antibiotic substitution
Answer: b) Antibiotic cycling
25. Which of the following is NOT a common strategy used by bacteria to resist the action of antibiotics?
a) Increasing antibiotic uptake
b) Modifying antibiotic targets
c) Inactivating antibiotics
d) Decreasing antibiotic efflux
Answer: a) Increasing antibiotic uptake
26. What is the primary reason behind the development of antibiotic resistance in agriculture?
a) The use of antibiotics to treat sick animals
b) The use of antibiotics as growth promoters
c) The absence of antibiotics in animal agriculture
d) The implementation of strict regulations on antibiotic use
Answer: b) The use of antibiotics as growth promoters
27. Which of the following is a key strategy to prevent the spread of antibiotic resistance within healthcare
facilities?
a) Frequent use of broad-spectrum antibiotics
b) Strict hand hygiene and infection control measures
c) Prescribing antibiotics for viral infections
d) Using antibiotics without a prescription
Answer: b) Strict hand hygiene and infection control measures
28. What is the term for a bacterium that is resistant to all known antibiotics and poses a significant threat
to public health?
a) Antibiotic-sensitive bacterium
b) Antibiotic-tolerant bacterium
c) Extensively drug-resistant bacterium
d) Antibiotic-resistant bacterium
Answer: c) Extensively drug-resistant bacterium
29. Which class of antibiotics is known for its activity against Mycobacterium tuberculosis, the bacterium
that causes tuberculosis (TB)?
a) Beta-lactams
b) Aminoglycosides
c) Quinolones
d) Rifamycins
Answer: d) Rifamycins
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30). How can plasmids contribute to drug resistance in bacteria?
a) By encoding antibiotic drugs
b) By providing a physical barrier to antibiotics
c) By carrying genes that confer resistance to antibiotics
d) By blocking antibiotic entry into bacterial cells
Answer: c) By carrying genes that confer resistance to antibiotics
32. Which of the following is an example of a plasmid-encoded resistance gene often found in bacteria?
a) Tetracyclinas
b) Penicillinase
c) Streptomycinase
d) Ampicillinase
33. Plasmid-mediated drug resistance can spread between bacteria of different species through a process
called:
a) Binary fission
b) Conjugation
34. Which of the following antibiotics is often affected by plasmid-mediated drug resistance mechanisms?
a) Antivirals
b) Antifungals
c) Beta-lactam antibiotics
35. What is the primary advantage of plasmid-mediated drug resistance for bacteria?
a) Increased susceptibility to antibiotics
b) Enhanced growth rate
c) Improved DNA replication
d) Survival in the presence of antibiotics
Microbiology
1. What is the primary mechanism of action of antibiotics that target bacterial cell walls?
A) Inhibition of DNA replication
B) Disruption of cell membrane integrity
C) Inhibition of protein synthesis
D) Inhibition of cell wall synthesis
Answer: D) Inhibition of cell wall synthesis
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3. Which of the following antibiotics interferes with protein synthesis by binding to the 30S ribosomal subunit
in bacteria?
A) Penicillin
B) Ciprofloxacin
C) Tetracycline
D) Vancomycin
Answer: C) Tetracycline
6. Which class of antimicrobial drugs targets fungal infections by disrupting the ergosterol component of
fungal cell membranes?
A) Aminoglycosides
B) Azoles
C) Cephalosporins
D) Quinolones
Answer: B) Azoles
7. What is the primary mechanism of action of antiviral drugs that target reverse transcriptase in
retroviruses?
A) Inhibition of viral entry
B) Inhibition of viral fusion
C) Inhibition of viral DNA replication
D) Inhibition of viral RNA synthesis
Answer: C) Inhibition of viral DNA replication
8. Which antimicrobial drug is commonly used to treat tuberculosis (TB) by inhibiting the synthesis of
mycobacterial cell wall components?
A) Ampicillin
B) Rifampin
C) Gentamicin
D) Fluconazole
Answer: B) Rifampin
9. What is the primary mechanism of action of antifungal drugs that target the synthesis of beta-glucans in
fungal cell walls?
A) Inhibition of RNA polymerase
B) Inhibition of ergosterol synthesis
C) Inhibition of beta-glucan synthesis
D) Inhibition of protein synthesis
Answer: C) Inhibition of beta-glucan synthesis
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10. Which of the following antibiotics disrupts bacterial cell membranes by creating channels or pores in the
lipid bilayer?
A) Penicillin
B) Ciprofloxacin
C) Polymyxin
D) Tetracycline
Answer: C) Polymyxin
11. How do antiprotozoal drugs like metronidazole work against parasitic infections?
A) Inhibition of DNA replication
B) Inhibition of cell wall synthesis
C) Inhibition of protein synthesis
D) Inhibition of RNA synthesis
Answer: A) Inhibition of DNA replication
12. Which antimicrobial drug class is known for inhibiting the activity of topoisomerases in bacteria,
preventing DNA supercoiling?
A) Aminoglycosides
B) Macrolides
C) Quinolones
D) Sulfonamides
Answer: C) Quinolones
13. Which antiviral drug inhibits the action of the viral enzyme protease, preventing the cleavage of viral
polyproteins into functional proteins?
A) Zanamivir
B) Oseltamivir
C) Ritonavir
D) Acyclovir
Answer: C) Ritonavir
14. What is the primary mechanism of action of aminoglycoside antibiotics such as gentamicin?
A) Inhibition of RNA polymerase
B) Inhibition of cell wall synthesis
C) Inhibition of protein synthesis
D) Inhibition of DNA replication
Answer: C) Inhibition of protein synthesis
15. Which antimicrobial drug class targets the 50S ribosomal subunit in bacteria, interfering with protein
synthesis?
A) Macrolides
B) Penicillins
C) Fluoroquinolones
D) Sulfonamides
Answer: A) Macrolides
16. How do antiviral drugs like acyclovir work against herpes simplex viruses (HSV)?
A) Inhibition of viral entry
B) Inhibition of viral fusion
C) Inhibition of viral DNA polymerase
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D) Inhibition of viral reverse transcriptase
Answer: C) Inhibition of viral DNA polymerase
17. Which antimicrobial drug is effective against both gram-positive and gram-negative bacteria and disrupts
bacterial cell membranes?
A) Chloramphenicol
B) Vancomycin
C) Polymyxin
D) Cephalosporin
Answer: C) Polymyxin
18. What is the primary mechanism of action of antifungal drugs known as echinocandins?
A) Inhibition of DNA replication
B) Inhibition of ergosterol synthesis
C) Inhibition of chitin synthesis
D) Inhibition of protein synthesis
Answer: C) Inhibition of chitin synthesis
19. Which antiparasitic drug disrupts the mitochondrial electron transport chain in parasites, leading to their
death?
A) Mebendazole
B) Artemisinin
C) Atovaquone
D) Ivermectin
Answer: C) Atovaquone
20. What is the primary mechanism of action of penicillin and other beta-lactam antibiotics?
A) Inhibition of DNA replication
B) Inhibition of RNA synthesis
C) Inhibition of protein synthesis
D) Inhibition of cell wall synthesis
Answer: D) Inhibition of cell wall synthesis
21. Which antiviral drug is commonly used to treat HIV by inhibiting the reverse transcriptase enzyme?
A) Ribavirin
B) Zidovudine (AZT)
C) Amantadine
D) Griseofulvin
Answer: B) Zidovudine (AZT)
22. How do antifungal drugs like amphotericin B work to kill fungal cells?
A) Inhibition of ergosterol synthesis
B) Inhibition of cell wall synthesis
C) Inhibition of RNA polymerase
D) Inhibition of DNA replication
Answer: A) Inhibition of ergosterol synthesis
23. Which antibiotic class is known for disrupting bacterial protein synthesis by binding to the 50S ribosomal
subunit?
A) Aminoglycosides
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B) Tetracyclines
C) Penicillins
D) Quinolones
Answer: B) Tetracyclines
24. What is the primary mechanism of action of metronidazole, an antibiotic used to treat anaerobic bacterial
infections and protozoal infections?
A) Inhibition of DNA gyrase
B) Inhibition of cell wall synthesis
C) Inhibition of DNA replication
D) Inhibition of RNA synthesis
Answer: C) Inhibition of DNA replication
25. Which antiviral drug targets the viral neuraminidase enzyme, preventing the release of newly formed
virions from infected cells?
A) Ritonavir
B) Oseltamivir
C) Acyclovir
D) Ribavirin
Answer: B) Oseltamivir
26. How do antimalarial drugs like chloroquine work to combat Plasmodium parasites?
A) Inhibition of protein synthesis
B) Inhibition of DNA replication
C) Inhibition of heme detoxification
D) Inhibition of cell wall synthesis
Answer: C) Inhibition of heme detoxification
27. Which class of antimicrobial drugs includes drugs like isoniazid and ethambutol used in the treatment of
tuberculosis (TB)?
A) Aminoglycosides
B) Quinolones
C) Antifolates
D) Antituberculosis drugs
Answer: D) Antituberculosis drugs
28. What is the primary mechanism of action of daptomycin, an antibiotic used to treat gram-positive bacterial
infections?
A) Inhibition of DNA replication
B) Inhibition of RNA synthesis
C) Disruption of the cell membrane
D) Inhibition of protein synthesis
Answer: C) Disruption of the cell membrane
29. Which antiparasitic drug is used to treat head lice and scabies by interfering with nerve transmission in
parasites?
A) Mebendazole
B) Pyrantel pamoate
C) Permethrin
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D) Praziquantel
Answer: C) Permethrin
30. What is the primary mechanism of action of mupirocin, an antibiotic used for skin infections?
A) Inhibition of RNA synthesis
B) Inhibition of DNA replication
C) Inhibition of protein synthesis
D) Inhibition of cell wall synthesis
Answer: D) Inhibition of cell wall synthesis
31. Which antiviral drug inhibits the activity of the HIV enzyme integrase, preventing the integration of viral
DNA into the host genome?
A) Zidovudine (AZT)
B) Raltegravir
C) Lamivudine
D) Ribavirin
Answer: B) Raltegravir
32. How do antifungal drugs like griseofulvin work against dermatophyte infections?
A) Inhibition of DNA replication
B) Inhibition of RNA synthesis
C) Inhibition of ergosterol synthesis
D) Inhibition of protein synthesis
Answer: A) Inhibition of DNA replication
33. Certainly! Here are some additional multiple-choice questions (MCQs) on antimicrobial drugs and their
mechanisms of action:
34. Which antibiotic class includes drugs like cephalexin and ceftriaxone, known for their broad-spectrum
activity against both gram-positive and gram-negative bacteria?
A) Penicillins
B) Macrolides
C) Cephalosporins
D) Sulfonamides
Answer: C) Cephalosporins
35. What is the primary mechanism of action of polymyxin B, an antibiotic used as a last-resort treatment for
multidrug-resistant gram-negative bacteria?
A) Inhibition of DNA replication
B) Inhibition of RNA synthesis
C) Disruption of the cell membrane
D) Inhibition of protein synthesis
Answer: C) Disruption of the cell membrane
36. Which antiviral drug interferes with the viral RNA-dependent RNA polymerase, inhibiting the replication of
RNA viruses like hepatitis C?
A) Ribavirin
B) Zanamivir
C) Sofosbuvir
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D) Nevirapine
Answer: C) Sofosbuvir
37. How do antiprotozoal drugs like chloroquine work against protozoal parasites like Plasmodium species?
A) Inhibition of DNA replication
B) Inhibition of protein synthesis
C) Inhibition of cell wall synthesis
D) Inhibition of RNA synthesis
Answer: A) Inhibition of DNA replication
38. Which class of antimicrobial drugs is primarily used to treat urinary tract infections and works by inhibiting
bacterial dihydrofolate reductase?
A) Penicillins
B) Tetracyclines
C) Sulfonamides
D) Aminoglycosides
Answer: C) Sulfonamides
39. What is the primary mechanism of action of nitrofurantoin, an antibiotic commonly used for urinary tract
infections?
A) Inhibition of DNA replication
B) Inhibition of cell wall synthesis
C) Disruption of the cell membrane
D) Inhibition of protein synthesis
Answer: A) Inhibition of DNA replication
40. Which antiviral drug targets the viral protease enzyme and is used to treat hepatitis C infections?
A) Oseltamivir
B) Ritonavir
C) Ledipasvir
D) Ribavirin
Answer: C) Ledipasvir
41. How do antifungal drugs like fluconazole work to treat fungal infections?
A) Inhibition of ergosterol synthesis
B) Inhibition of RNA synthesis
C) Inhibition of chitin synthesis
D) Inhibition of protein synthesis
Answer: A) Inhibition of ergosterol synthesis
42. Which antimicrobial drug is used to treat leprosy (Hansen’s disease) by inhibiting the synthesis of mycolic
acids in Mycobacterium species?
A) Rifampin
B) Streptomycin
C) Dapsone
D) Isoniazid
Answer: D) Isoniazid
43. What is the primary mechanism of action of nystatin, an antifungal drug used to treat candidiasis and
other fungal infections?
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A) Inhibition of ergosterol synthesis
B) Inhibition of RNA synthesis
C) Inhibition of chitin synthesis
D) Inhibition of DNA replication
Answer: A) Inhibition of ergosterol synthesis
Laboratory Diagnosis
1. Which staining technique is commonly used to differentiate bacterial species into Gram-positive and
Gram-negative groups?
a) Acid-fast staining
b) Capsule staining
c) Flagella staining
d) Gram staining Answer: d) Gram staining
3. What is the primary goal of PCR (Polymerase Chain Reaction) in microbiology diagnosis?
a) Identifying bacterial morphology
b) Amplifying and detecting specific DNA sequences
c) Measuring antibiotic susceptibility
d) Determining serological reactions Answer: b) Amplifying and detecting specific DNA sequences
6. If the venipuncture site is inadequately disinfected, a blood culture bottle is most often contaminated with
which kind of bacteria?
a) E.coli
b) Staph epidermidis
7. Which laboratory technique allows for the identification of microorganisms based on their genetic
material?
a) Gram staining
b) PCR (Polymerase Chain Reaction)
c) Serological testing
d) MALDI-TOF mass spectrometry Answer: b) PCR (Polymerase Chain Reaction)
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8. What is the primary purpose of MALDI-TOF mass spectrometry in microbiology?
a) Amplifying DNA sequences
b) Identifying fungal spores
c) Analyzing protein profiles for bacterial and fungal identification
d) Determining bacterial motility Answer: c) Analyzing protein profiles for bacterial and fungal
identification
10. In a microbiology laboratory, what is the primary purpose of using biosafety cabinets?
a) Sterilizing laboratory equipment
b) Ensuring laboratory personnel wear PPE
c) Providing a controlled environment for handling microorganisms
d) Amplifying DNA sequences Answer: c) Providing a controlled environment for handling
microorganisms
11. Which of the following is a safety measure to protect against biohazardous materials in a microbiology
laboratory?
a) Wearing lab coats
b) Using glassware without gloves
c) Working in an open laboratory environment
d) Disposing of hazardous materials in regular waste bins Answer: a) Wearing lab coats
12. Which of the following techniques involves the cultivation of microorganisms on selective media to
encourage the growth of specific species?
a) Streak plating
b) Enrichment culturing
c) Gram staining
d) Acid-fast staining Answer: b) Enrichment culturing
14. Which of the following is NOT a common application of molecular diagnostics in microbiology?
a) Identifying the genetic basis of antibiotic resistance
b) Detecting viral RNA in blood samples
c) Analyzing bacterial colony morphology
d) Investigating the genetic diversity of microbial populations Answer: c) Analyzing bacterial colony
morphology
15. What is the primary purpose of the Kirby-Bauer disk diffusion method in microbiology?
a) Identifying bacterial species
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b) Measuring bacterial motility
c) Assessing antibiotic susceptibility
d) Detecting fungal infections Answer: c) Assessing antibiotic susceptibility
16. Which of the following laboratory techniques is used to visualize the structural components of viruses?
a) Gram staining
b) Electron microscopy
c) MALDI-TOF mass spectrometry
d) PCR (Polymerase Chain Reaction) Answer: b) Electron microscopy
17. What type of microscope is commonly used for examining live microorganisms in a microbiology
laboratory?
a) Scanning electron microscope (SEM)
b) Transmission electron microscope (TEM)
c) Phase-contrast microscope
d) Confocal microscope Answer: c) Phase-contrast microscope
18. In a microbiology laboratory, what does the term “aseptic technique” refer to?
a) The use of antimicrobial agents to sterilize equipment
b) Proper handling and manipulation of microorganisms to prevent contamination
c) The use of antibiotics to inhibit bacterial growth
d) Rapid culture growth under high-pressure conditions Answer: b) Proper handling and manipulation of
microorganisms to prevent contamination
19. Which of the following is NOT a category of personal protective equipment (PPE) used in microbiology
laboratories?
a) Lab coats
b) Safety goggles
c) Hairnets
d) Office attire Answer: d) Office attire
21. What is the main purpose of a biosafety level (BSL) designation in a microbiology laboratory?
a) To indicate the level of cleanliness in the laboratory
b) To specify the types of microorganisms that can be handled safely
c) To determine the color of laboratory equipment
d) To regulate laboratory funding Answer: b) To specify the types of microorganisms that can be handled
safely
22. Which of the following staining techniques is commonly used to detect Mycobacterium tuberculosis in
clinical samples?
a) Gram staining
b) Acid-fast staining
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c) Flagella staining
d) Capsule staining Answer: b) Acid-fast staining
24. Which diagnostic method is most suitable for identifying the presence of viral RNA in a patient’s blood
sample?
a) Gram staining
b) Serological testing
c) PCR (Polymerase Chain Reaction)
d) Metagenomics Answer: c) PCR (Polymerase Chain Reaction)
26. What is the primary advantage of using MALDI-TOF mass spectrometry for microbial identification?
a) It provides information about bacterial motility.
b) It allows for the visualization of bacterial colonies.
c) It provides rapid and accurate identification based on protein profiles.
d) It measures antibiotic sensitivity. Answer: c) It provides rapid and accurate identification based on
protein profiles.
27. Which of the following microscopes is most suitable for studying the internal structures of microorganisms
in detail?
a) Scanning electron microscope (SEM)
b) Transmission electron microscope (TEM)
c) Phase-contrast microscope
d) Confocal microscope Answer: b) Transmission electron microscope (TEM)
28. What does the term “aseptic technique” emphasize in microbiology laboratory practices?
a) Maintaining a sterile environment
b) Rapidly culturing microorganisms
c) Using selective media
d) Identifying microorganisms based on DNA sequencing Answer: a) Maintaining a sterile environment
29. Which of the following is NOT a common component of personal protective equipment (PPE) used in
microbiology laboratories?
a) Lab coat
b) Safety goggles
c) Hairnet
d) Office attire Answer: d) Office attire
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30. In microbiology, what is the primary function of using selective media?
a) To encourage the growth of all microorganisms
b) To promote rapid bacterial replication
c) To inhibit the growth of specific microorganisms
d) To enhance bacterial motility Answer: c) To inhibit the growth of specific microorganisms
31. What does the biosafety level (BSL) designation of a laboratory indicate?
a) The laboratory’s location
b) The level of funding provided to the laboratory
c) The types of microorganisms that can be safely handled in the laboratory
d) The laboratory’s cleanliness standards Answer: c) The types of microorganisms that can be safely
handled in the laboratory
Host Defenses
1. What is the primary function of the skin in host defense against microbial pathogens?
a) Temperature regulation
b) Sensation
c) Physical barrier
d) Nutrient absorption
Answer: c) Physical barrier
3. Which type of white blood cell is primarily responsible for the ingestion and destruction of
invading microbes?
a) T cells
b) B cells
c) Neutrophils
d) Platelets
Answer: c) Neutrophils
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d) Skin barrier
Answer: c) Antibody production
6. Immunization with a vaccine primarily activates which branch of the immune system?
a) Innate immune system
b) Adaptive immune system
c) Both innate and adaptive immune systems
d) None of the above
Answer: b) Adaptive immune system
9. Mucous membranes in the respiratory and digestive tracts are part of the host defense
mechanism primarily due to:
a) Antibody secretion
b) Coughing and sneezing reflexes
c) Mucus production and ciliary action
d) Enzyme secretion
Answer: c) Mucus production and ciliary action
10. Which of the following is a mechanism by which pathogens can evade host defenses?
a) Activation of phagocytes
b) Production of antibodies
c) Antigen presentation
d) Antigenic variation
Answer: d) Antigenic variation
11. What is the primary function of the spleen in the immune system?
a) Blood clotting
b) Red blood cell production
c) Filtering and monitoring blood for pathogens
d) Digestion of pathogens
Answer: c) Filtering and monitoring blood for pathogens
12. Which type of immune response is typically faster upon re-exposure to a previously
encountered pathogen?
a) Primary immune response
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b) Secondary immune response
c) Tertiary immune response
d) Innate immune response
Answer: b) Secondary immune response
14. The process by which immune cells recognize self from non-self is called:
a) Antigen presentation
b) Immune memory
c) Self-tolerance
d) Immunization
Answer: c) Self-tolerance
15. Which class of antibodies is most abundant in the bloodstream and is the first to be
produced during an immune response?
a) IgA
b) IgE
c) IgG
d) IgM
Answer: d) IgM
16. What is the primary function of cytotoxic T cells in the immune system?
a) Producing antibodies
b) Phagocytosis of pathogens
c) Directly killing infected cells
d) Regulating inflammation
Answer: c) Directly killing infected cells
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19. Which of the following is a major function of the lymphatic system in host defense?
a) Oxygen transport
b) Nutrient absorption
c) Drainage of interstitial fluid and transport of immune cells
d) Regulation of body temperature
Answer: c) Drainage of interstitial fluid and transport of immune cells
21. Which of the following is NOT a component of the first line of defense in the immune
system?
a) Skin
b) Mucous membranes
c) Fever
d) Saliva
Answer: c) Fever
22. Which type of white blood cell is primarily responsible for presenting antigens to T cells
during an immune response?
a) Neutrophils
b) B cells
c) Macrophages
d) Natural killer cells
Answer: c) Macrophages
23. The process by which antibodies and complement proteins coat the surface of
pathogens, making them easier to be recognized and phagocytosed, is called:
a) Opsonization
b) Antigenic variation
c) Immunization
d) Autoimmunity
Answer: a) Opsonization
25. Which class of antibodies is involved in allergic reactions and defense against
parasites?
a) IgA
b) IgG
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c) IgE
d) IgM
Answer: c) IgE
26. Which of the following is a function of the lymph nodes in the immune system?
a) Filtering and monitoring blood
b) Maturation of T cells
c) Production of red blood cells
d) Digestion of pathogens
Answer: b) Maturation of T cells
28. What is the primary role of helper T cells in the immune system?
a) Directly kill infected cells
b) Produce antibodies
c) Coordinate immune responses
d) Phagocytose pathogens
Answer: c) Coordinate immune responses
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2. What anticoagulant is typically found in red-topped blood collection tubes?
a) Heparin
b) EDTA
c) Sodium citrate
d) None
Answer: d) None
3. Which color of bottle is often used for coagulation testing in clinical laboratories?
a) Green
b) Purple
c) Blue
d) Gray
Answer: c) Blue
8. What anticoagulant is commonly found in pink-topped blood collection tubes for blood
cultures?
a) Heparin
b) EDTA
c) Sodium citrate
d) SPS (Sodium Polyanethol Sulfonate)
Answer: d) SPS (Sodium Polyanethol Sulfonate)
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9. Which color of bottle is used for hematology testing, including complete blood counts
(CBC)?
a) Green
b) Red
c) Lavender
d) Gray
Answer: c) Lavender
11. Which color of bottle is commonly used for chemistry tests, such as electrolytes and
liver function tests, in clinical laboratories?
a) Green
b) Red
c) Lavender
d) Gray
Answer: b) Red
13. Which color of bottle is used for blood gas analysis, including pH and pCO2
measurements?
a) Green
b) Blue
c) Brown
d) Gray
Answer: c) Brown
14. What anticoagulant is commonly found in brown-topped blood collection tubes for blood
gas analysis?
a) Heparin
b) EDTA
c) Sodium citrate
d) Heparin with gel separator
Answer: d) Heparin with gel separator
15. Which color of bottle is used for trace element testing, such as zinc and copper levels?
a) Yellow
b) Gray
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c) Black
d) Royal blue
Answer: d) Royal blue
16. What anticoagulant or additive is typically found in royal blue-topped blood collection
tubes for trace element testing?
a) Heparin
b) EDTA
c) Sodium citrate
d) None (no anticoagulant)
Answer: d) None (no anticoagulant)
17. Which color of bottle is used for blood typing and compatibility testing?
a) Blue
b) Pink
c) Gray
d) Orange
Answer: b) Pink
18. What anticoagulant is commonly found in pink-topped blood collection tubes for blood
typing and compatibility testing?
a) Heparin
b) EDTA
c) Sodium citrate
d) ACD
Answer: c) Sodium citrate
19. Which color of bottle is used for collecting samples for drug testing, including toxicology
screenings?
a) Green
b) Red
c) Gray
d) Gold (Tiger top)
Answer: d) Gold (Tiger top)
20. What additive is typically found in gold (Tiger top) blood collection tubes for drug
testing?
a) Heparin
b) EDTA
c) Gel separator with clot activator
d) Sodium citrate
Answer: c) Gel separator with clot activator
21. Which color of bottle is commonly used for collecting samples for serology tests, such
as HIV or syphilis tests?
a) Red
b) Blue
c) Yellow
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d) Black
Answer: c) Yellow
22. What anticoagulant is typically found in yellow-topped blood collection tubes for
serology testing?
a) Heparin
b) EDTA
c) Sodium citrate
d) ACD
Answer: d) ACD (Acid Citrate Dextrose)
23. Which color of bottle is used for erythrocyte sedimentation rate (ESR) testing?
a) Green
b) Red
c) Purple
d) Black
Answer: c) Purple
24. What anticoagulant is commonly found in purple-topped blood collection tubes for ESR
testing?
a) Heparin
b) EDTA
c) Sodium citrate
d) ACD
Answer: b) EDTA
25. Which color of bottle is used for collecting samples for blood bank crossmatching?
a) Green
b) Red
c) Pink
d) Black
Answer: b) Red
26. What type of additive is typically found in blood bank collection tubes for
crossmatching?
a) Heparin
b) EDTA
c) Sodium citrate
d) No additive, just a clot activator
Answer: d) No additive, just a clot activator
27. Which color of bottle is used for special coagulation tests, such as D-dimer or
fibrinogen assays?
a) Green
b) Blue
c) Light blue
d) Gray
Answer: c) Light blue
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28. What anticoagulant is commonly found in light blue-topped blood collection tubes for
special coagulation tests?
a) Heparin
b) EDTA
c) Sodium citrate
d) Sodium polyanethol sulfonate (SPS)
Answer: c) Sodium citrate
29. Which color of bottle is used for collecting samples for lead testing?
a) Green
b) Red
c) Lavender
d) Royal blue
Answer: d) Royal blue
30. What additive is typically found in royal blue-topped blood collection tubes for lead
testing?
a) Heparin
b) EDTA
c) Sodium citrate
d) None (no additive)
Answer: d) None (no additive)
31. Which color of bottle is typically used for collecting samples for toxicology testing, such
as drug screens?
a) Green
b) Red
c) Gray
d) Orange
Answer: d) Orange
32. What additive is commonly found in orange-topped blood collection tubes for toxicology
testing?
a) Heparin
b) EDTA
c) Gel separator with clot activator
d) Sodium citrate
Answer: c) Gel separator with clot activator
33. Which color of bottle is used for collecting samples for blood ethanol (alcohol) testing?
a) Green
b) Red
c) Gray
d) Light blue
Answer: d) Light blue
34. What anticoagulant or additive is typically found in light blue-topped blood collection
tubes for blood ethanol testing?
a) Heparin
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b) EDTA
c) Sodium citrate
d) Sodium fluoride
Answer: d) Sodium fluoride
35. Which color of bottle is used for collecting samples for arterial blood gas (ABG)
analysis?
a) Green
b) Blue
c) Brown
d) Gray
Answer: b) Blue
36. What anticoagulant or preservative is commonly found in blood collection tubes for
arterial blood gas (ABG) analysis?
a) Heparin
b) EDTA
c) Sodium citrate
d) Heparin with gel separator
Answer: a) Heparin
37. Which color of bottle is used for collecting samples for immunology tests, such as
autoimmune or allergy panels?
a) Red
b) Blue
c) Yellow
d) Gold (Tiger top)
Answer: b) Blue
38. What anticoagulant is typically found in blue-topped blood collection tubes for
immunology testing?
a) Heparin
b) EDTA
c) Sodium citrate
d) None (no anticoagulant)
Answer: d) None (no anticoagulant)
39. Which color of bottle is used for collecting samples for cytogenetics or molecular
genetics testing?
a) Red
b) Blue
c) Yellow
d) Gray
Answer: d) Gray
40. What additive is commonly found in gray-topped blood collection tubes for cytogenetics
or molecular genetics testing?
a) Heparin
b) EDTA
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c) Sodium citrate
d) Special preservative solutions
Answer: d) Special preservative solutions
Pathogenesis
Question 1: What is the term for the ability of a microorganism to cause disease?
A) Virulence
B) Immunity
C) Sterility
D) Pathogenicity
Answer: A) Virulence
A) The microorganism must be isolated from a diseased individual and grown in pure culture.
B) The microorganism must cause disease when introduced into a healthy individual.
C) The microorganism must be visible under a light microscope.
D) The microorganism must be re-isolated from the experimentally infected individual.
Question 3: Which term refers to the ability of a microorganism to cause severe disease in a
host?
A) Infectivity
B) Virulence
C) Pathogenicity
D) Contagiousness
Answer: B) Virulence
A) Lipopolysaccharide (LPS)
B) Streptolysin S
C) Lipoteichoic acid
D) Peptidoglycan
Answer: B) Streptolysin S
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D) Endotoxins are structural components of the outer membrane of Gram-negative bacteria,
while exotoxins are secreted proteins.
Question 6: Which type of pathogen can establish an infection only if the host’s immune
system is compromised?
Question 7: Which of the following is an example of a direct transmission route for infectious
diseases?
Question 9: Which term describes a state of unresponsiveness to antigens from one’s own
body?
A) Immunity
B) Allergy
C) Autoimmunity
D) Tolerance
Answer: D) Tolerance
Question 10: Which of the following is a mechanism that bacteria use to evade the host
immune system?
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C) Antigenic variation
D) Enhancing phagocytosis
A) Influenza
B) Tuberculosis
C) Common cold
D) Streptococcal throat infection
Answer: B) Tuberculosis
Question 14: Which of the following is a vector-borne transmission route for infectious
diseases?
Question 15: What is the primary function of MHC molecules in the immune response?
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Question 16: Which of the following is a virulence factor that allows bacteria to resist
phagocytosis by immune cells?
A) Antibodies
B) Complement proteins
C) Phagolysosomes
D) Capsule formation
Question 18: Which type of toxin causes the symptoms of fever, inflammation, and shock in
response to Gram-negative bacterial infections?
A) Exotoxins
B) Enterotoxins
C) Lipopolysaccharides (LPS)
D) Endotoxins
Answer: D) Endotoxins
A) Measles
B) Chickenpox
C) Acute bacterial meningitis
D) HIV/AIDS
Answer: D) HIV/AIDS
Question 20: How do vaccines primarily protect individuals from infectious diseases?
Question 21: Which of the following best describes a reservoir in the context of infectious
diseases?
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A) An organism that carries a pathogen without showing symptoms of the disease
B) A place where pathogens are produced in large quantities
C) A laboratory where microbial cultures are maintained
D) A region with a high prevalence of a specific disease
Answer: A) An organism that carries a pathogen without showing symptoms of the disease
Question 23: What is the term for a microorganism’s genetic makeup, which can influence its
ability to cause disease?
A) Virulence factors
B) Phenotype
C) Pathogenicity islands
D) Genotype
Answer: D) Genotype
Question 25: What is the primary role of the mucociliary escalator in preventing respiratory
infections?
Question 26: Which type of toxin targets nerve cells and can lead to symptoms like muscle
paralysis?
A) Enterotoxin
B) Cytotoxin
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C) Neurotoxin
D) Hemolysin
Answer: C) Neurotoxin
Question 28: What role do cytokines play in the immune response to infection?
A) Athlete’s foot
B) Urinary tract infection
C) Tuberculosis
D) Strep throat
Answer: C) Tuberculosis
Question 30: Which of the following is a common portal of entry for pathogens?
A) Healthy skin
B) Intact mucous membranes
C) Respiratory tract
D) Intact digestive system
Question 31: Which type of immune response involves antibodies produced by B cells?
A) Cell-mediated immunity
B) Innate immunity
C) Humoral immunity
D) Passive immunity
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A) Candida albicans
B) Escherichia coli
C) Influenza virus
D) Mycobacterium tuberculosis
Question 33: What is the primary function of the complement system in the immune
response?
A) Producing antibodies
B) Destroying infected host cells
C) Enhancing phagocytosis
D) Promoting inflammation
A) To neutralize toxins
B) To prevent phagocytosis
C) To stimulate antibody production
D) To promote attachment to host cells
Question 36: What is the term for a population in which a specific disease regularly spreads?
A) Pandemic
B) Endemic
C) Epidemic
D) Outbreak
Answer: B) Endemic
A) Staphylococcus aureus
B) Streptococcus pyogenes
C) Escherichia coli
D) Clostridium botulinum
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Answer: C) Escherichia coli
A) Producing antibodies
B) Regulating T-cell responses
C) Phagocytosing pathogens and debris
D) Recognizing antigens on infected cells
Question 39: Which of the following is an example of a foodborne transmission route for
infectious diseases?
Question 40: What is the primary function of memory cells in the immune response?
A) To produce antibodies
B) To initiate inflammation
C) To recognize and respond to repeat infections
D) To differentiate into various immune cell types
A) Lipopolysaccharide
B) Polypeptide
A) lipopolysaccharide
B) Polypeptide
Question 43: Traveler’s diarrhea (Watery, none bloody diarrhea) caused by the?
A) E.coli
B) Labile toxin
C) Both
Question 44: Bloody diarrhea associated with undercooked hamburgers caused by which
kinds of toxin?
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A) Verotoxin
B) Shiga Like Toxin
C) BOTH
A) Endotoxin
B) Exotoxin
Question 46: Which toxin produced by both gram positive and gram negative?
A) Endotoxin
B) Exotoxin
A) Endotoxin
B) Exotoxin
Question 48: Hand washing is an important means of interrupting the chain of transmission
from ONE person to ANOTHER?
A) Saph Aureus
B) Strep Agalactiae
Classification of bacteria
Question 1: Which of the following bacterial groups possess a thick layer of peptidoglycan in
their cell walls, making them Gram-positive?
A) Proteobacteria
B) Cyanobacteria
C) Firmicutes
D) Spirochetes
Answer: C) Firmicutes
Question 2: Bacteria with a thin layer of peptidoglycan in their cell walls, which stains pink in
Gram staining, are referred to as:
A) Gram-negative
B) Gram-positive
C) Gram-neutral
D) Gram-variable
Answer: A) Gram-negative
Question 3: The bacterial group that includes species with diverse shapes such as cocci,
bacilli, and spiral forms, and possess an outer membrane in their cell wall structure, is:
A) Actinobacteria
B) Firmicutes
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C) Proteobacteria
D) Cyanobacteria
Answer: C) Proteobacteria
Question 4: Which bacterial phylum comprises organisms that are often found in extreme
environments, have a unique cell wall structure, and include the genus Thermus?
A) Chloroflexi
B) Thermotogae
C) Aquificae
D) Deinococcus-Thermus
Answer: D) Deinococcus-Thermus
Question 5: The bacterial group known for their branching filaments, often forming complex
mycelium-like structures, and having a high G+C content in their DNA, is:
A) Cyanobacteria
B) Spirochetes
C) Actinobacteria
D) Chlamydiae
Answer: C) Actinobacteria
Question 6: Which of the following bacterial groups is characterized by a unique cell wall
structure containing pseudomurein, and they are often found in extreme environments like hot
springs and hydrothermal vents?
A) Firmicutes
B) Spirochetes
C) Crenarchaeota
D) Thaumarchaeota
Answer: C) Crenarchaeota
Question 7: The bacterial group responsible for nitrogen fixation and often found in root
nodules of leguminous plants belongs to which phylum?
A) Cyanobacteria
B) Proteobacteria
C) Spirochetes
D) Chlamydiae
Answer: B) Proteobacteria
Question 8: The genus Borrelia which causes diseases like Lyme disease is a member of
which bacterial group?
A) Firmicutes
B) Actinobacteria
C) Spirochetes
D) Thermotogae
Answer: C) Spirochetes
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Question 9: Which bacterial phylum includes species that lack a peptidoglycan cell wall and
are characterized by a unique cell membrane composed of ether-linked lipids?
A) Planctomycetes
B) Fusobacteria
C) Bacteroidetes
D) Chlamydiae
Answer: A) Planctomycetes
Question 10: The bacterial group responsible for many sexually transmitted infections and
includes the genus Chlamydia is:
A) Firmicutes
B) Actinobacteria
C) Spirochetes
D) Chlamydiae
Answer: D) Chlamydiae
Question 11: Bacteria belonging to the phylum Cyanobacteria are known for:
A) Having no cell walls
B) Being Gram-negative
C) Performing photosynthesis
D) Being thermophilic
Question 13: Which bacterial group is responsible for causing tuberculosis and leprosy?
A) Actinobacteria
B) Cyanobacteria
C) Chlamydiae
D) Thermotogae
Answer: A) Actinobacteria
Question 14: The bacterial phylum Chloroflexi includes species that are commonly found in:
A) Extreme thermophilic environments
B) Oxygen-rich environments
C) Anaerobic environments
D) Saline environments
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Question 15: Which bacterial phylum includes species responsible for producing methane as
a metabolic byproduct?
A) Bacteroidetes
B) Thermotogae
C) Firmicutes
D) Euryarchaeota
Answer: D) Euryarchaeota
Question 16: Bacteria of the phylum Fusobacteria are often associated with infections
involving:
A) Gastrointestinal tract
B) Respiratory tract
C) Urinary tract
D) Oral cavity
Question 17: Which bacterial group includes the genus Mycoplasma known for their lack of a
cell wall?
A) Firmicutes
B) Actinobacteria
C) Spirochetes
D) Tenericutes
Answer: D) Tenericutes
Question 19: The phylum Aquificae includes thermophilic bacteria that generate energy
through:
A) Photosynthesis
B) Methanogenesis
C) Sulfur oxidation
D) Hydrogen oxidation
Question 20: The bacterial phylum Bacteroidetes is commonly found in which type of
environments?
A) Acidic hot springs
B) Extreme halophilic environments
C) Marine and freshwater habitats
D) Deep-sea hydrothermal vents
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Answer: C) Marine and freshwater habitats
Question 21: Gram-positive, cocci, cause abscesses of skin are the characteristics of which
bacteria.
A) Staphylococcus
B) Streptococcus
C) E.coli
Question 22: Gram-positive, aerobic, spore-forming rods are the characteristics of which
bacteria.
A) Bacilius
B) Clostridium
C) E.coli
Question 23: Gram-negative, cocci, cause gonorrhea are the characteristics of which
bacteria?
A) Staphylococcus
B) Neisseria
C) E.coli
Question 24: Gram-negative, rod, respiratory organisms cause whooping cough are the
characteristics of which bacteria?
A) Hemophilus
B) Bordetella
C) E.coli
Question 25: Gram-negative, rod, enteric related cause typhoid fever are the characteristics
of which bacteria?
A) Shagella
B) Salmonella
C) Proteus
Question 26: Gram-negative, curved shape, cause peptic ulcer, gastric ulcer are the
characteristics of which bacteria?
A) Campylobactor
B) Helicobacter
C) E.coli
Question 27: Obligatory intracellular parasites, cause Q-fever, and typhus are the
characteristics of which bacteria?
A) Rickettsia
B) Chlamydia
C) None
Question 28: Wallless cells, cause Pneumonia are the characteristics of which bacteria?
A) Mycoplasma
B) Treponema
C) Leptospira
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mutation, conjugation, transduction,
transformation
Question 1: What type of mutation involves the insertion or deletion of a single
nucleotide, leading to a shift in the reading frame of the gene?
A) Silent mutation
B) Missense mutation
C) Nonsense mutation
D) Frameshift mutation
A) Silent mutation
B) Nonsense mutation
C) Missense mutation
D) Frameshift mutation
A) Chemical mutagen
B) Radiation mutagen
C) Spontaneous mutagen
D) Replicative mutagen
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Answer: B) Radiation mutagen
Question 6: A mutation that changes a codon encoding an amino acid to a stop codon
is called:
A) Silent mutation
B) Missense mutation
C) Nonsense mutation
D) Frameshift mutation
Question 7: Which repair mechanism specifically targets incorrect base pairs that
occur due to errors during DNA replication?
A) Mismatch repair
B) Nucleotide excision repair
C) Base excision repair
D) Direct repair
A) Inducible operon
B) Repressible operon
C) Constitutive operon
D) Nonsense operon
Question 10: What is the term for the process by which bacteria acquire new genetic
material from their environment or from other bacteria?
A) Transduction
B) Transformation
C) Conjugation
D) Replication
Answer: B) Transformation
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Question 11: Which of the following is a heritable mutation that occurs in gametes and
can be passed on to offspring?
A) Somatic mutation
B) Spontaneous mutation
C) Germline mutation
D) Induced mutation
Question 12: A mutation that has no detectable effect on the phenotype of an organism
is called:
A) Silent mutation
B) Nonsense mutation
C) Missense mutation
D) Frameshift mutation
Question 14: Which of the following is a type of mutagenic chemical that mimics a DNA
base and can be incorporated into the DNA strand during replication, causing mistakes
in base pairing?
A) UV radiation
B) X-rays
C) Intercalating agent
D) Alkylating agent
Question 15: A mutation in a gene that leads to the loss of its function is termed:
A) Gain-of-function mutation
B) Nonsense mutation
C) Null mutation
D) Spontaneous mutation
Question 16: The process of DNA repair that involves removing a segment of damaged
DNA and replacing it with the correct sequence is known as:
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A) Mismatch repair
B) Base excision repair
C) Nucleotide excision repair
D) Direct repair
Question 17: What type of mutation occurs when one or more nucleotides are added or
deleted from the DNA sequence, causing a shift in the reading frame?
A) Silent mutation
B) Missense mutation
C) Nonsense mutation
D) Frameshift mutation
Question 18: The trp operon is an example of which type of gene regulation?
A) Inducible operon
B) Repressible operon
C) Constitutive operon
D) Nonsense operon
Question 19: Which repair mechanism involves the direct reversal of DNA damage,
such as the removal of alkyl groups from DNA bases?
A) Mismatch repair
B) Base excision repair
C) Nucleotide excision repair
D) Direct repair
Question 20: Mutations in the BRCA1 and BRCA2 genes are associated with an
increased risk of:
A) Heart disease
B) Diabetes
C) Breast and ovarian cancer
D) Lung cancer
A) Plasmid
B) Pilus
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C) Capsule
D) Flagellum
Answer: B) Pilus
Question 22: Which type of genetic material is commonly transferred through bacterial
conjugation?
A) Chromosomal DNA
B) Ribosomal RNA
C) Mitochondrial DNA
D) Plasmid DNA
A) Transformation
B) Conjugation
C) Horizontal gene transfer
D) Viral vectors transferring DNA
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Answer: D) It only transfers specific bacterial genes located near the phage integration
site.
Transformation:
Answer: C) The uptake and incorporation of foreign DNA into a bacterial cell.
A) Plasmid
B) Capsule
C) Pilus
D) Competence factor
Question 30: What is the primary function of the F (fertility) plasmid in bacterial
conjugation?
Question 31: Which bacterial genus is known for its ability to undergo conjugation,
leading to the transfer of antibiotic-resistance genes?
A) Escherichia
B) Bacillus
C) Streptococcus
D) Clostridium
Answer: A) Escherichia
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Question 32: In generalized transduction, which type of genetic material can be
transferred from one bacterium to another?
Question 33: Which term refers to the process where bacterial genes located near the
integrated phage DNA are transferred to a recipient bacterium via a transducing phage
particle?
A) Specialized transduction
B) Generalized transduction
C) Conjugative transduction
D) Fragmented transduction
Question 34: In natural transformation, what is required for the bacterial cell to uptake
foreign DNA?
Question 35: Which of the following bacterial species is well known for its ability to
undergo natural transformation?
A) Staphylococcus aureus
B) Mycobacterium tuberculosis
C) Streptococcus pneumoniae
D) Clostridium botulinum
Question 36: What term describes the genetic material taken up from the environment
by a bacterial cell and incorporated into its genome?
A) Conjugation
B) Transduction
C) Transformation
D) Replication
Answer: C) Transformation
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Question 37: Which bacterial group is commonly associated with the ability to transfer
antibiotic resistance genes through conjugation?
A) Gram-positive bacteria
B) Acid-fast bacteria
C) Obligate anaerobic bacteria
D) Cyanobacteria
A) Plasmid
B) Flagellum
C) Pilus
D) Capsule
Answer: C) Pilus
Question 39: Which type of transduction involves the accidental packaging of host
DNA into a phage particle during the lytic cycle?
A) Generalized transduction
B) Specialized transduction
C) Conjugative transduction
D) Directed transduction
Question 40: What is the term for a bacterial cell that is susceptible to being infected
by a transducing phage and receiving foreign DNA?
A) Lytic cell
B) Non-receptive cell
C) Competent cell
D) Conjugative cell
Question 41: Which discovery is often credited to Frederick Griffith’s experiment using
Streptococcus pneumoniae?
A) Bacterial conjugation
B) Bacterial transduction
C) Bacterial transformation
D) Bacterial replication
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Question 42: In bacterial transformation, what is the process that involves the uptake
of naked DNA fragments from the environment?
Question 43: What does the term “competence” refer to in the context of bacterial
transformation?
Answer: C) The physiological state enabling bacteria to uptake and incorporate foreign
DNA
Question 44: What is the basic unit of heredity that carries genetic information?
a) Protein
b) Amino Acid
c) Nucleotide
d) Carbohydrate
Answer: c) Nucleotide
Question 45: Which scientist is known as the “Father of Genetics” for his work on pea
plants?
a) Isaac Newton
b) Gregor Mendel
c) Charles Darwin
d) Louis Pasteur
Question 46: What is the term for the specific location of a gene on a chromosome?
a) Allele
b) Locus
c) Genotype
d) Phenotype
Answer: b) Locus
Question 47: A trait that is expressed only when an individual has two identical alleles for a
particular gene is called:
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a) Dominant trait
b) Recessive trait
c) Co-dominant trait
d) Incomplete dominance
a) Down syndrome
b) Hemophilia
c) Turner syndrome
d) Cystic fibrosis
a) Thymine (T)
b) Cytosine (C)
c) Guanine (G)
d) Uracil (U)
Question 49: What is the term for a change in the DNA sequence of a gene?
a) Mutation
b) Transcription
c) Translation
d) Replication
Answer: a) Mutation
Question 50: The process by which genetic information is copied from DNA to RNA is called:
a) Translation
b) Replication
c) Transcription
d) Transduction
Answer: c) Transcription
Question 51: Which genetic disorder is characterized by an extra copy of chromosome 21,
leading to intellectual disabilities and certain physical features?
a) Hemophilia
b) Down syndrome
c) Sickle cell anemia
d) Muscular dystrophy
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Answer: b) Down syndrome
Question 52: What is the term for the observable characteristics or traits of an organism?
a) Genotype
b) Phenotype
c) Allele
d) Mutation
Answer: b) Phenotype
04. New Methylene Blue Reagent is used for staining of which blood cells?
a. Reticulocytes.✔
b. Platelets.
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06. When WBC are counted manually by turk’s Reagent, Blood is diluted by Ratio ?
1:20
1:50
1:100
1:200
08. Which is the following chemical test differentiate between Staphylococcus and Streptococcus
a. Ammoniase Test
b. Catalase Test✔
c. Urase Test
d. Oxidase Test
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Magnesium
Zinc
15. Which is the following hormone directly Regulate Blood Glucose Level?
TSH
FSH
Progesterone
Insulin✓
16. Which is the following Anticoagulant Tube need to collect sample of HbA1c ?
Sodium Flouride
EDTA✔
Heparin
Sodium Sitrate
18. A Blood Donor who recently diagnoses Positive HbsAg should be deffered for how long?
Permanantly✔
For 3 months
For 6 Months
For 1 Year
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19. Which is the following is called “bad” Cholestrol?
Cholestrole
Triglyceride
HDL
LDL✔
20. Which one of the following test is used to differentiate Staphylococcus aureus from other Stapl
Urase Test
Coagulase Test✔
Oxidase Test
22. Salmonella and Shigella can be differentiate on the base of which test?
Gram Stain
Catalase Test
Motility✓
Indole Test
23. Ammonium Oxalate reagent is used for the counting of which test?
WBC’s
Platelets ✓
RBC’s
Reticulocytes
24. Which of the following Bio-chemistry Test Should keep away from light?
Bilirubin✔
Glucose
calcium
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27. The solution used to fix a pap smear is:
wright stain
hematoxylin
physiological saline
cytospray✔
28. Which on of the following bacteria can cause glomerular nephritis or FR?
Staphylococcus aureus
Streptococcus Pyogenes✔
Staphylococcus Epidermidis
Streptococcus Pneumonia
30. in DIC all of the following test will show high results except?
PT
Platelets✓
APTT
None of the above
31. Three types of ketone bodies include acetoacetic acid, acetone and __?
Oxaloacetic acid
Lactic Acid
3-hydroxy Butilic acid ✔
Acietic Acid
32. The degree that a control results deviate from known valus or mean value is known as :-
Standard Deviation✔
Coefficient Deviation
Median
None of above
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Hemophilia
Leukemia
34. One of the following enzyme also performed cardiac enzyme function?
Acid Phosphatase
Creatinine
LDH✔
ALT
35. ESR will show low value result in which one of the following condition :-
Polycythemia✔
Anemia
RA
Tuberculosis
38. Which One of the Following bacteria is gram negative, Aerobic oxidase positive and motile
rod?
E-Coli
Proteus
Salmonella
Pseudomonas✔
39. Which one of the following cells play a major role in humoral immunity?
B-Cells✔
T-Cells
Monocytes
Macrophages
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40. In Iron Deficiency anemia laboratory finding which one of the following will show increased
value?
Hb
Iron
TIBC✓
RBC’s
42. If Sample is taken in EDTA Tube, Which one of the following biochemistry test results will
affect?
Glucose . Urea
Cholestrol
Calcium ✔
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b. Stimulates the bone-marrow to produce RBC’s 3. Is released in responce to hypoxemia
c. All of above✔
48. Each of following characteristics is correct about Ascaris lumbricoides except one :-
a. Ascaris lumbricaid is nematode
b. Both dogs and cats are intermediate host of Ascaris lumbricoides✔
c. Ascaris lumbricoides can cause pneumonia
d. Ascaris lumbricoides is transmetted by enjections of eggs
51. When myocardial infarction will happen which of the following cardiac enzyme will elevate
LDH
CK✓
Clinical microbiology
1. Chose the gram-positive bacteria.
a. Neisseria,
b. Klebsiella,
c. Clostridium
2. Free-living, Thick wall, cause abscess of skin. Characteristics of.
a. Streptococcus
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b. Staphylococcus
c. Bacillus
3. Extracellular bacteria, thick wall, spore-forming aerobe Characteristics of.
a. Clostridium
b. Corynebacterium
c. Bacillus
4. Gram-negative cocci are.
a. Neisseria
b. Haemophilus
c. Bacillus
5. Gram-negative, rod, Facultative, straight are the Characteristics of.
a. Haemophilus
b. Neisseria
c. Yersinia
6. Respiratory gram-negative bacteria is.
a. Bordetella
b. Brucella
c. Yersinia
7. Zoonotic Organism is.
a. Pasteurella
b. boradetell
c. E.coli
8. Which bacteria cause typhoid fever.
a. Proteus
b. Salmonella
c. Shigella
9. What is the pathogenic factor of Peptic ulcer?
a. Vario
b. Campylobacter
c. Helicobactor
10. Q-fever caused by the ?
a. Chlamydia
b. Rickettsia
c. Vario
11. The causative agent of Diphtheria is.
a. listeria
b. Nocardia
c. Corynebacterium
12. Chose important normal flora of the skin.
a. Epidermidis
b. E.coli
c. Lactobacillus
13. Flexible, thin-walled cells (spirochetes), Lyme disease is the characteristics of.
a.Treponema
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b. Borrelia
c. Leptospirara
14. Normal flora is the organisms that are the.
a. Permanent resident of body
b. Came outside
c. Normal cells
15. Yeast can be normal flora.
a. True
b. false
16. Commensals are.
a. Get benefits from their host and cause damage to them
b. Get benefits from their host but did no damage to them
c. Free-living
17. Important normal flora of colon is.
A. e.coli
b. Fragilis
c. Viridin
18. An outbreak of post-surgical wound infection caused by staph aureus has
occurred in the hospital. Use your knowledge of normal flora. Which of the following
sites is the most likely location for staph aureus.
a. Throat
b. Nose
c. Mouth
19. Staph aureus is present on the skin but the main site of it is.
a. Colon
b. Nose
c. Mouth
20. Which part of the body contains the highest number of flora.
a. Mouth
b. Stomach
c. Colon
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C. Erlenmeyer cylinder
D. graduate cylinder
I. Safety bulb
Answer: a) Volumetric flash
7. Solid crystals of potassium oxalate are mixed in distilled water in a container. Potassium oxalate
is described by which term?
A. Solution
B. solvent
C. molten material
D. Regent
I. AC
Answer: c) Solvent
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Answer: c) Sterilization
15. Which part of the histology equipment does not depend on temperature:
A. Wax
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Bishu processor
C
D. Embedding Center
I. Water bath
Answer: c) Microtome
16. Biopsy:
A. Expulsion of organic liquid
B. Displacement
C. Post mortem examination
D. Representative tissue pattern
I. Blood collection
Answer: D) Excision of a representative tissue sample
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Answer: a) Decatur disk
28. If Lavender Top, Plane Red Top, Gray Top and Light Blue Top Tube are collected, what is the
order of the draw:
A. They can be collected in any order.
B. Plain red top, lavender, blue, gray
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C. Blue, Plain Red Top, Gray, Lavender
D. Gray, Blue, Lavender, Plain red top
I. Plain red top, light blue, lavender, gray
Answer: e) Plain red top, light blue, lavender, gray
30. If the patient falls unconscious at the time of the injury, you must:
A. Call the doctor once
B. Remove the needle and present it to the patient
C. Shout out loud
Continue this process until all blood is collected
I. Start artificial respiration immediately
Answer: b) Remove the needle and leave the patient
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35. Latex Gloves Protect Laboratory Worker:
A. Sudden needle puncture
B. minor injury
C. Patient Aerosol
D. Body fluid
I. All of the above
Answer: D) body fluids
39. Blend well before testing blood samples for cell count:
A. Avoid Platelets Clamp
B. Avoid small clots
C. Give oxygen to the sample
D. Also determine the distribution of all blood components
I. A mixture of anticoagulants with blood
Answer: D) Also determine the distribution of all blood components
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D. 2.8 mm
I. 3.0 mm
Answer: C) 2.4 mm
42. The first drop of blood is removed after the skin is punctured:
A. Remove any existing pathogens
B. Increased blood flow to this area
C. Remove the last trace of alcohol
D. Remove any excess tissue fluid
I. C&D
Answer: e) C and D
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A. crenated
B. Liphemic
C. Jiggar
D. Illness
I. Hemolyzed
Answer: D) Iconic
50. A sample:
A. Spread the material on the slide
Blood or urine volume
C. A small sample taken to represent the whole organism or system
D. a microbial colony that grows on solid medium
I. The technique used for microscopic examination of urine
Answer: c) a small sample taken to represent the whole organism or system
53. Identify the correct sequence of steps on the gram stain process:
A. Primary stain, secondary stain, mordant, decolorizing
B. mordant, primary stain, decomposition, forgery
C. Counterstain, mordant, primary stain, decolorizing
D. Primary stain, merodant, decorating, counter stain
I. None of these
Answer: d) Primary stain, mordant, decolorizing, counter stain
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I. B&D
Answer: e) b and d
60. When processing the swab, body fluids or blood are most commonly used for protection:
A. Fume hood
B. Square L Laminar Flow Hood
C. Square ll Laminar Flow Hood
D. Square LL Laminar Flow Hood
I. Glove Box
Answer: C) Class L Laminar Flow Hood
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61. You are collecting blood sugar levels. The patient asks if you think he has diabetes. You’ll tell
him:
A. It is a possibility but you are not positive
B. You cannot give him any information
C. He can answer this question by discussing it with the doctor
D. You are instructed not to give any information, so you cannot discuss it
I. Read a prepared booklet and make your decision
Answer: c) Discuss this question with a therapist because he can answer this question.
65. Dilute 1/6 of serum in water. Glucose Outcome 4.0 mmol / L. What is the reported result:
A. 0.66 mmol / L.
B. 4.0 mmol / L.
C. 24.0 mmol / L.
D. 40.0 mmol / L.
I. 60.0 mmol / L.
Answer: C) 24.0 mmol / L
66. How many ml of 4% hydrochloric acid will make 100 ml of 20% hydrochloric acid:
A. 50 ml.
B. 80 ml.
C. 100 ml.
D. 500 ml.
I. 1000 ml.
Answer: D) 500 milliliters
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B. 4 grams
C. 6 g
D. 20 grams
I. Unable to identify with the information
Answer: c) 6 grams
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