OBJECTIVE

To see if it is possible to
clone cauliflower
 PROBLEM

Question / Problem: What is

better for a cauliflower
explant to be able to grow?
Commercial or Homemade
agar.

HYPOTHESIS
 We hypothesize that
cauliflower explants will
grow better and faster with
the commercial agar when
being exposed to direct
sunlight.

VARIABLES
 Controlled variable: the
cauliflower growing in a
normal environment

Dependent variable: the

length of the cauliflower,
time it takes to grow, and
the sun.

Independent variable:
commercial and homemade
agar.
MATERIALS
 1 bottle of Alcohol (70%)
 2 Beakers for waste solutions
  Cauliflower head
 Cauliflower florets cut into 10 mm
pieces from a fresh whole
cauliflower
 agar
 1 Cutting board
 1 Knife
 2 tweezers
 1 Glass marking pencil
 3 Paper towels rolls
 3 Scalpels
 Sterling in a glass jar with lid
 Sterling solution in beaker

EXPERIMENTAL
PROCEDURE
1. Collect a small floret of cauliflower, and
place it on a cutting board.
2. Using a scalpel, cut the mini-floret into
pieces of approximately 3–5 mm3. These are
your explants.
3. Every 2–3 minutes, swirl the jar gently for 5
seconds. Repeat until 15 minutes have passed.
4. Place the forceps back into the beaker of
sterilizing solution.
5. Using a paper towel and a small amount of
70% alcohol, wipe down the cutting board.
Repeat these steps for the three trials.

TRIAL ONE
OBSERVATIONS
AND RESULTS
12/16/2022
Washing materials
1. Started to mix the agar solution with water
and put it directly into the plates and leave it
in the fridge for 20 minutes

2. After 20 minutes had passed, we noticed that

the agar wasn’t changing its consistency, so
we decided to let it sit for more time. The
instructions for the powdered agar said that
we had to steam the formula, so we did it and
let it set for 20 minutes again

3. When the agar got the right consistency, we

put the cut cauliflower and put them right in
the sun so they can grow.

TRIAL TWO
OBSERVATIONS
AND RESULTS
12/30/22
1. We waited for a certain amount of time to see
the cauliflower. we could see that the plant grew
and it looked healthy.

2. We measured it and saw it was two inches

long. We decided to let it rest and see if it would
continue growing.

TRIAL THREE
OBSERVATIONS
AND RESULTS
01/6/2023

1. After letting our little plant rest

for a week, we checked on it and
saw that our plant was black and it
was dead. We realized that our
homemade agar didn't work
therefore our hypothesis was wrong.
GRAPH

Pictures
 CONCLUSION
In conclusion, our
homemade agar didn’t work
for us, but the commercial
agar did since it grew a little
but in the end, it died. We
still recommend trying this
project because maybe it
didn't work for us but
maybe it can work for you if
you use another type of
material.
APPLICATION
 This project can be useful
for people who can't afford
commercial agar or if you
ran out of your commercial
agar and you need it
immediately you can do a
homemade agar because it's
very affordable and
efficient.