Rishav Saravanan

Hu and Meng

5 July 2023

Research Internship

Using Nuclear Magnetic Resonance Spectroscopy for Plant Analysis

As we attempt to focus on combatting the critical issues of plant growth and maturation,

there are a large number of different effects and impacts as it comes to the different worlds of

ecology, biodiversity, and a variety of different relationships that require plants as a critical factor

to their environmental growth. This paper investigates the chief rationale of how we can use

Nuclear Magnetic Resonance Spectroscopy (NMR) in order to benefit the growth of plants and

increase the crop yield of plants in order to help regions of low socio-economic regions and

benefit their agricultural access to food and resources. This can be done with the use of

increasing metabolites, which are sources of energy, which aid in plant growth and maturation.

In addition, I will be experimenting with different fertilizer samples in each different plant group.

In a study conducted by Nowak et. All from the Northern Research Station, a plant

survey population was given 254 black locusts, 199 southern magnolia, and 27 London plane

trees. Given the starting report of time t=2 years, around 34% of the population size had died.

Annually, the report informed 19% of the survey population had died. Given this population risk

and effect, the annual support for each member of the plant species had been devastating, with

alarming negative net effects. In particular, areas of lower socio-economic levels report even

less crop yield annually. Nuclear Magnetic Resonance is a data collection method for structure
determination using atomic bonding structures and magnetic fields to map out metabolites

necessary for plant cell division, growth, respiration, and reproduction. The production of

metabolites is critical to plant health and nutrition.

Figure 0: Influence of current environmental factors on the crop yield, SOURCE: U.S. Global Research Program

In areas of low socio-economic levels and any climate alike, millions of planted trees do

not receive the proper nourishment needed to survive and thrive. Due to this reason, I will be

using a method called Nuclear Magnetic Resonance Spectroscopy to analyze materials of

glucose, and other cellular compounds that play a major role in the growth of plants and

organisms. Using spectroscopy to analyze the chemical compounds, I will derive and identify

sources that lead to possible weaknesses in each glucose chemical bond and analyze the

metabolites in the samples. Using this information from the samples, I will grow a simulated

physical mock environment of metabolite-rich resources to examine the quality of products

(vegetables and fruits) produced by the metabolite-rich nutrient.

My experimentation will use already existing metabolite-based compounds to simulate

the environmental goal of gaining metabolite-rich content for plant growth. The experimentation
will model how effectively tracking metabolite-level activity will allow for plant growth and

maturation. In my experiment, I will set up a time period for testing and growing plants, given a

model of metabolite-based resources.

1. CONT: Control Group: Create a fertilizer sample. This sample will be used for where the

control group plants will grow. These plants will not receive metabolite-rich nutrients and

will be given traditional resources for growth.

2. EXP: Experimental Group: Create another fertilizer sample, however, this sample will

include metabolite-rich content including banana peels, blueberries, and a variety of

nutrients promoting metabolite growth which allows for plant growth and plant

maturation at a healthier and accelerated pace, because excess energy improves the

production of metabolites for plants.

3. During a period of 2 months, I will be growing plants, and on a weekly basis, I will

measure and evaluate differences and changes between both the control group and the

extra-metabolite group.

While there are no harmful chemicals, the procedure will not require any risk or safety

measurement needed for experimentation. Using this data, I will analyze the growth of the

metabolite-based compounds given the sample to evaluate plant growth. From this experiment, I

was able to conclude that – based off of the NMR Readings – both nitric, anaptic, and carboxylic

acids were critical to the development of Metabolite Function and Development. Using these

materials in the development of soil not only increase the quality of produce created, but they

also mathematically provide a stronger constant for proportion hence accelerating the growth of

these plants.

Figure 1: Sampling Data from the Experimentation SOURCE: Rishav Saravanan

Figure 2: Graphs from Derivative Equations, using data from experimentation. SOURCE: Rishav Saravanan

Figure 3: Research Gate: Nuclear Magnetic Resonance Readings of Glucose, Glutamate, and a variety of other

Glucose-Based Polymers

Using the data from the experimentation process that I had set up, I was best able to simulate the

growth of plants. Using the data points and a constant of proportionality, the growth rate of

each plant was best modeled through:

𝚺dy/dx=ke^t
Hence, using the data from the equation, I was able to conclude the following about the research.

I was able to effectively note that the modified fertilizer had a specific increase in its growth rate

of up to about 1.2 times as effective in the growth rate of the plant. Using our conclusions drawn

from Research Gate, I was able to conclude that anaptic, citric, and modified plants best

accelerate the growth of plants. Using this information, the research that I have conducted has a

large implication onto the field of environmental studies. Through the use of the NMR readings,

I can now conclude that the use of the three crucial acids, commonly found in modified

fertilizers, will aid in the development of creating stronger plants, and also increasing the rate at

which these plants grow. Using this information, we can now modify fertilizers to include these

acids found in multiple vegetables and plants into modern fertilizers and implement them into

fields of low socio-economic areas. Doing so will increase the productivity at which we can

grow these plants.

 Works Cited

❖ Emwas, Abdul-Hamid, et al. “NMR Spectroscopy for Metabolomics Research.”

Metabolites, U.S. National Library of Medicine, 27 June 2019,

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6680826/.

❖ Lee, Jeong Hyun, et al. “Using the Expolinear Growth Equation for Modelling Crop

Growth in Year-Round Cut Chrysanthemum.” Annals of Botany, U.S. National Library of

Medicine, Nov. 2003, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4244859/.

❖ https://www.researchgate.net/figure/NMR-based-H9c2-cell-metabolomic-analysis-A-typi

cal-600-MHz-1-H-NMR-spectra-obtained_fig2_324224766