GENE EXPRESSION

Transcriptome. Northern blot measure levels of mRNA in tissue for example. Typically I can test a
large number of samples. Once, instead of separation by dimension, I can probe separation by dot
blot. The big problem is, as long I put an mRNA on filter I have The big problem is, as long I put an
mRNA on filter, I have 1 filter for 1 gene: 10 gene  10 genes and it’s ok, but if I have 10000
genes it’s not possible to have so much filters. This is based on the idea I put the whole genome of
an entire tissue.
But what if I put the genome of a single cell? I can attach my probe on the filter by chemical
reactions, and other fancy techniques, or I can use fancy nucleotide in sense that are marked, they
can produce color. The concept of Microarray is every spot on the chip contains a probe for each
mRNA.
I want to know more what family of gene is more expressed instead of a single gene. That’s because
a single gene level is more subject to errors, so it’s better to consider genes of a family, groups of
genes.