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1
Title:
Document No: Page No.
SOP/QC/038 SOP for Preparation of Microbial Culture Media Page 1 of 4
Prepared by: X X
Department Name Date
Checked by: Y Y
Department Name Date
Approved by: Z Z
Department Name Date
1. Purpose
Company Name:: Issue No.
1
Title:
Document No: Page No.
SOP/QC/038 SOP for Preparation of Microbial Culture Media Page 2 of 4
This procedure defines the operating procedure to prepare the culture media and preparation
of plates & slants
2. Scope
This SOP is valid only until next revision date and if it bears control seal.
3. Responsibility
It will be the responsibility of the Microbiologist - Quality Controlto follow the procedure.
Quality assurance manager is responsible for SOP compliance.
Balance
Dehydrated media
Autoclave
Laminar air flow
Glassware
6. Procedure:
6.3.4. Unload the tubes from the autoclave and place them in a slanting position so as to achieve
a slant and put the tubes as such in a test tube stand for Butts.
6.3.5. Ensure that the top of the slant does not touch the cotton plug and allow to solidify.
6.3.6. Pre-incubate for 48 hours at the appropriate temperature.
6.3.7. Check visually for any contamination. Discard if necessary.
6.3.8. Mark the tubes appropriately with the Name of medium, Lot no., and Preparation date.
6.3.9. Store the slants, after pre-incubation below 25°C.
6.4. Preparation of RODAC Plates:
6.4.1. Rehydrate the medium and sterilize as per the media preparation instructions.
6.4.2. Cool the media and pour in sterile RODAC plates under aseptic conditions. The RODAC
plates shall be marked on the bottom position with the details of the name of the medium,
lot no., date of preparation.
6.5. Traceability of Media / Sterilization Load Numbers
6.5.1. Each lot of medium prepared and sterilized is assigned a specific lot number as 8
characters as given below.
XX-YYZZM
XX : indicates Serial no. of prepared media
YY : indicates Month
ZZ : indicates Year
M : indicates the abbreviation of the media.
6.5.2. All media lots prepared / sterilized are to be serially numbered month wise. Record the
name of medium and specific lot number of all media used for microbiological testing in
the Analytical Data Sheet.
6.5.3. Organisms for Positive Control 7/29/2017 SOP for Preparation of Microbial Culture
Media : Bacillus subtilis NCIM 2063 (or equivalent)
Candida albicans NCIM 3471 (or equivalent)
For differential and selective media inoculate the medium with respective micro
organisms as mentioned on the container having concentration of less than 100 cfu / ml.