Tosoh Automated

Glycohemoglobin Analyzer

β-Thalassemia Analysis Mode

β-Thalassemia Analysis Mode
Measurement of HbA 2 and HbF, which are minor
components of human Hb, is useful for the
differential diagnosis of several anemia and
thalassemias (thal). Specifically, an increased
HbA 2 value is considered to be a β-thal trait.
The HLC-723 ®G11(G11), high performance liquid
chromatography (HPLC) with a cation exchange
resin column is available for HbA 2 measurement
with good precision of results and saving time.

・HbF and HbA 2 results in 5 minutes

・High quality chromatographic resolution,

eliminating interference especially from HbS,
HbC, HbD and HbE
・Detect high concentration of HbF (up to 50 %)
・HbA 2 Inter- and Intra-assay CV’s < 2.0 %

・Easy to exchange the column & filter

・Guide screen

・Handy barcode scanner (Option)

・Flag function
・User account management
・Reagent & Maintenance management

・Small footprint reduces required bench space

Performance Data
Sample Chromatogram

■ Normal ■ β-Thal. trait ■ HbAS ■ HbAE

HbF
HbA 0
HbA 2

High Accuracy

■ Intra-assay (n=20) ■ Inter-assay (n=20)

HbF HbA2 HbF HbA2
Level 1 Level 2 Level 1 Level 2 Level 1 Level 2 Level 1 Level 2
MEAN 1.50 5.12 3.19 6.48 MEAN 1.51 4.69 3.17 5.78
SD 0.00 0.04 0.06 0.04 SD 0.02 0.03 0.06 0.11
CV % 0.00 0.78 0.94 0.62 CV % 1.32 0.64 1.89 1.90

Correlation between G8 and G11

HbA2 (%)
7
y = 0.98x + 0.05
6 r = 1.00
n = 51

5
HLC-723G11

0
0 1 2 3 4 5 6 7 8
HLC-723G8 In-house data
SPECIFICATIONS PART NUMBERS / DESCRIPTION
Analytes HbF, HbA 2 0023850 HLC-723G11 (Main Unit)

Applicable samples Whole blood and diluted samples 0023851 HLC-723G11LA (Main Unit) *

Assay principle Ion exchange high performance liquid chromatography 0023852 G11-90SL (90 Sample Loader)

Detection method 2-wavelength absorbance (detection wavelength: 415 nm) 0023853 G11-290SL (290 Sample Loader)

Processing throughput 5 min/sample * Possible to connect with Laboratory Automation Belt line
β-Thalassemia Analysis Mode
Main unit
Column connection Finger-tight-type 0023504 TSKgel G11 β-Thal. (Analysis Column)

Sampling volume 6 µL for whole blood and 150 µL for diluted samples 0023505 G11 β-Thalassemia Elution Buffer No. 1 (S)
Sample aspiration by Nozzle 0023506 G11 β-Thalassemia Elution Buffer No. 2 (S)
0023507 G11 β-Thalassemia Elution Buffer No. 3 (S)
Pump unit Single plunger pump
0018431 HSi Hemolysis & Wash Solution (L)
System control / Data processing
0019550 HSi Hemolysis & Wash Solution (LL)
Display 320 × 240 dot matrix color LCD
0023508 G11 F&A2 Calibrator Set
Input Pressure-sensitive touch panel / operation keys
0023509 G11 F&A2 Control Set
Output Thermal printer
Storage USB stick Analysis Column Elution Buffers and
Hemolysis & Wash Solutions
Data processing unit RS-232C serial communication port (bi-directional)
Data storage by internal memory (for up to 800 samples)
Recalculation (reprinting) of achieved result
Automatic startup by timer
Error flag function for abnormal results
Calibration 2-point calibration by calibrators
Data extraction Extract by date or sample type (max 800 samples)
Control for quality control Distinguished by barcode ID (max 4 controls) G11 F&A2 Calibrator Set G11 F&A2 Control Set

Sampling unit
Sample loading capacity 90 samples or 290 samples
Sample rack 10 primary tubes or cups per rack
Sample dilution Dilution by Hemolysis & Wash Solution in the dilution port
Sample tubes or cups 12 - 15 mm diameter × 75 - 100 mm primary tubes / Sample cups
Sample ID recognition Barcode with maximum of 20 digits
Barcode specifications NW-7 (Codabar), CODE39, ITF and CODE128 (initial setting), or JAN
(UPC/EAN), Industrial 2 of 5 and COOP 2 of 5 (requires setting change)

Temperature 15 - 30 °C
OPTIONS
0022944 Handy Barcode scanner
Power requirement AC100 - 240 V, 50 / 60 Hz, 200 VA
0023924 Base for Handy Barcode scanner
Dimensions / weight 90SL model : 530 (W) × 515 (D) × 482 (H) mm / 37 kg

290SL model : 1120 (W) × 530 (D) × 482 (H) mm / 54 kg

* “HLC-723” and “TSKgel” are the registered trademarks of Tosoh
Corporation in Japan, etc.
“G11” is the registered trademark of Tosoh Corporation in Japan.
* Specifications and design may be changed without prior notice.

TOSOH CORPORATION
Bioscience Division
3-8-2 Shiba, Minato-ku, Tokyo 105-8623, Japan
Phone : +81 3 5427 5181
Fax : +81 3 5427 5220

This brochure may not be reprinted or copied in whole or

in part without written consent from Tosoh Corporation.
Copyright 2017 by Tosoh Corporation

Printed in Japan 3708GX- Cat.No.0992359 A