Group 3 GAM Manuscript First Sem

WESTERN MINDANAO STATE UNIVERSITY
COLLEGE OF AGRICULTURE
DEPARTMENT OF AGRIBUSINESS
San Ramon, Zamboanga City
INTERNSHIP-PRACTICUM AT VARIOUS RESEARCH-BASED

PROJECTS IN THE WMSU-COLLEGE OF AGRICULTURE,
SAN RAMON, ZAMBOANGA CITY
INTERNSHIP-PRACTICUM MANUSCRIPT
Presented to the Faculty of the Agribusiness Department

in Partial Fulfillment of the Requirements for the degree of
BACHELOR OF SCIENCE IN AGRIBUSINESS
GOMEZ, RAPHAEL LANCE T.

MANALO, DIVINE GRACE N.
AYUNTING, BABIEGRACE M.
APRIL 2023
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In partial fulfillment of the requirement for the Degree of Bachelor of Science in
Agribusiness entitled: “Practicum Internship on Various Researched-Based Projects in
Western Mindanao State University, College of Agriculture, San Ramon Zamboanga City”
prepared and submitted by Mr. Raphael Lance T. Gomez, Ms. Divine Grace N. Manalo and Ms.
Babiegrace M. Ayunting. Is hereby recommended for oral defense.
MS. MERCELYN B. TILUS MRS. EMELYN D. VARONA

Adviser Adviser
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APPROVAL
In partial fulfillment of the requirement for the Degree of Bachelor of Science in

Agribusiness and with recommendation of the committee for the oral defense, the Manuscript
for Internship and Practicum presented by Ms. Gerry May Clemente, Mr. Raphael Lance
Gomez, and Ms. Joannah Mae L. Mahinay a BSAB-IV student on December 2022.
APPROVED.
SIGNATURE DATE
MS. MERCELYN B. TILUS _____________ _________
Adviser
MRS. EMELYN D. VARONA _____________ _________
Adviser
Approved and accepted in partial fulfillment of the requirements for the Degree of Bachelor of
Science in Agribusiness, December 2022
MRS. GLADESS P. PAGAL DR. ELDERICO P. TABAL

Department Head Dean-College of Agriculture
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GRAMMARIAN’S CERTIFICATE
This is to certify that the undersigned has reviewed and gone through all the pages of the
manuscript entitled "INTERNSHIP-PRACTICUM ON VARIOUS RESEARCHED-BASED
PROJECTS IN THE WESTERN MINDANAO STATE UNIVERSITY, COLLEGE OF
AGRICULTURE, SAN RAMON, ZAMBOANGA CITY" developed by MS. BABIEGRACE
M AYUNTING, MR. JORIS IAN LOUISE, and MS. DIVINE GRACE N. MANALO aligned
with the set of structural rules that govern the composition of sentences, phrases, and words in
the English language.
Signed this ___ day of _______ in the year ________ at _________________________

Zamboanga City.
Signature with name
4
ABSTRACT
Practicum-Internship is a requirement for all graduating BSAB student of the college. Its
objective is to expose students to different Agri-entrepreneur applying the theories,
techniques, philosophies and skills learned from various subjects.
This would also prepare them for future employment and income generation activities.
Last SY 2021-2022, the fourth year BSAB students were exposed to five production
activities, Whereas, this year, the proponents were assigned to four areas, as follows:
Zampen Native Chicken (IGP), Tissue culture Banana Lakatan production, Oyster
mushroom production, Food processing and market the available products.
The activities involved in varied agricultural production are described in this manuscript,
along with any issues that were encountered and how they were resolved. Financial
data statements are also included. This written piece was created not only for the
author's interest but also to aid readers in cultivating an open mind and a knowledge of
agriculture.
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ACKNOWLEDGEMENT
Grateful acknowledgement is hereby given to the following individuals for their
invaluable support which has contributed greatly to the realization and success of this study:
To our advisers Ms. Emilyn Varona and Ms. Mercelyn B. Tilus for selflessly lending
their expertise in the field of Agribusiness management skills and for their untiring help with
suggestions and correction to further improve of this study.
To our parents, for their financial support and unending encouragement and inspiration to
continue strive in our studies particularly in pursuing BS Agribusiness.
Special thanks are also extended to our classmates for their moral and generous support
during the rough moments in conducting this study.
Above all, to our Almighty God, for giving us strength, confidence, and strong faith to
surpass all the hardships during this schooling and for all the blessings He continue to bestow
upon us.
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Table of Contents
Approval sheet 3
Grammarian’s certificate 4
Abstract 5
Acknowledgement 6
Table of contents 7-8
List of tables 9
List of figures 10
CHAPTER I
A. ZAMPEN NATIVE CHICKEN-IGP
Introduction 11
Significance of the study 11
Internship’s Objectives 11
CHAPTER II
Actual undertakings/ Actual accomplishments 12
Technical aspect 13
Organizational chart 14
Actual activities accomplished 15-17
Other activities accomplished 17-19
Practices and management 20-26
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CHAPTER I
B. TISSUE CULTURE BANANA LAKATAN
Introduction 29-31
CHAPTER II
Actual undertakings/ Actual accomplishments 32-34
Practice management 45
Actual activities accomplished 47-48
CHAPTER III
Problems encountered and solution undertaken 49
CHAPTER IV
Conclusion 50
Recommendation 51
CHAPTER V
Learning experience 52
APPENDICES
Appendix A
Definition of terms 53-55
References 55
Appendix B
Author Biography 56-61
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CHAPTER I
I. Introduction
Background of the study
The BSAB (Bachelor of science in Agricultural business) 4 th students of Western
Mindanao State University- College have conducted their practicum in campus.
Zampen Natuve Chicken- IGP. The Zampen Native Chicken is a chicken breed
created and develop by Western Mindanao State university (WMSU) in partnership with
the Philippine council for Agriculture, Aquatic, and Natural Resources Research and
Development (PICAARD). The name comes from its Geographical location of origin, the
Zamboanga Peninsula. The interns were able to experienced the activities in Zampen
Native Chicken-IGP that includes of collection of eggs, feeding, loading and unloading
of eggs in the incubator, cleaning and maintaining the areas, fermentation of forages,
feed formulation mixing, vaccination of chicken, deworming of chicken, slitting,
defeathering, and slaughtering of chickens.
Tissue Culture, Banana Lakatan. The Western Mindanao State University’s Tissue
culture in partnership with Department of Agriculture (DA) started the Banana Lakatan
Tissue culture production on March 2021. The component of Tissue Culture Laboratory
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are the General washing area, heating room, chemical room, sterile room and
germination room. In which the interns were able to experienced the activities in the
different Tissue culture Laboratory areas.
Significance of the study
The study served as a resource, a manual, and a contribution to the students'

acquisition of various skills and knowledge in agriculture as well as their exposure to
various fields of endeavor in the future.
The research also significantly contributes by allowing the interns to improve their
marketing, technical, and planning skills through exposure to real-world projects like
Zampen Native Chicken-IGP and Tissue culture Banana Lakatan.
Internship’s Objectives
1. To be able to learn and manage the following researched-based projects
a. Zampen Native Chicken (Income Generating Project)

 To determine the profitability of Zampen native chicken project
 To gain knowledge in managing livestock farm
 To be able to identify pure breed Zampen native chicken
b. Banana Lakatan Tissue Culture Production
 To be able to formulate stock solutions and media preparation
 To be able to learn the basic process of tissue culture
 To learn the importance of tissue culture
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2. To identify the challenges and problems of the different areas assigned.

3. To overcome the problems identified in different areas assigned.
CHAPTER II
II. ACTUAL UNDERTAKINGS/ ACTUAL ACCOMPLISHMENTS
A. ZAMPEN NATIVE CHICKEN (Income Generating Project)

INTRODUCTION
The ZamPen Native Chicken is a chicken breed created and developed by Western
Mindanao State University (WMSU) in partnership with the Philippine Council for
Agriculture, Aquatic, and Natural Resources Research and Development (PCAARRD).
The name comes from its geographical location of origin, the Zamboanga Peninsula or
ZamPen.
The Zampen Native Chicken is a pure breed after undergoing a purification process.and
one of its most distinguishing characteristics is the taste of its meat. At present, the ZamPen
Native Chickens are not yet commercialized and the breeding stocks were mainly intended for
farmers who are willing and interested in raising this breed of chicken. The farmers who raise
and are willing to raise this breed are advised not to interbreed them with another breed of native
chicken as it will cause mongrelization, which may lead to decreasing its unique quality that was
developed through 10 years of research.
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The current main focus of the Zampen Native Chicken is to produce more which
leads them to make a study about artificial insemination which is still currently ongoing to
know if it has a higher fertilization rate than natural breeding. Another is their study about
the feed formulation for the specific stages of the chicken growing where they use 50%
commercial feeds and 50% forages to lessen the feed expenses while still giving them
enough nutrients for growth.
TECHNICAL ASPECT
Growth and Developmental Stages of Zampen Native Chicken-IGP
Incubating Candling
Breeder
0-18 days 0-7 days
7 months
Selected Hatching
Breeding
18-21 days
5 months - 7
months (RTL)
Selected Brooding
Breeding
Day old - 21
5 months - 7 days
months
(RTL)
Growing Hardening
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46-5 months 22-45 days
Figure 1. Growth and Developmental Stages of Zampen Native Chicken-IGP
Note: 3 years old breeder considered as Culled
ORGANIZATIONAL CHART
Zampen Native Chicken (Income Generating Project)
Dr. Elderico P.
Tabal
Project Leader
Mr. Crisanto
Floriano III
Project
Manager
Nina Tara
Ryan Levi Jalon Milter O. Diez
Iturralde
Farm Aid Farm Aid
Farm Aid
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ACTUAL ACTIVITIES ACCOMPLISHED
This table shows the list of the daily activities the interns had undertaken at Zampen
Native Chicken-IGP.
WEEK 1
Monday (January 23, 2023)
- Cleaning the area
- Fermenting of Feeds (upland swamp cabbage, molasses)
- Orientation
- Feeding management (commercial feeds mixed corn bran and concentrated EMAS.
- Collecting of Eggs
- Recording data of eggs (date today, and family)
Tuesday (January 24, 2023)

- Feeding
- Slaughtering
- Artificial Insemination (AI)
- Collection of Eggs
Wednesday (January 25, 2023)

- Cleaning and washing of the water container
- Feeding Management
- Vaccination
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- Vitamins and antibacterial/anti-infective

- Feeding
Thursday (January 26, 2023)

 Cleaning and disinfecting of the brooding area
Slitting
Transferred newly hatched in the brooding unit
Friday (January 27, 2023)
- Cleaning Management
WEEK 2
Monday (January 30, 2023)

- Disposing of the dead chickens
- Weeding
Tuesday (January 31, 2023)
- Vaccination
- Slaughtering
Wednesday (February 01, 2023)
- Feeding
- Mixing of Feeds
- Sacking of rice hulls
Thursday (February 02, 2023)
- Washing and refilling of the water container
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- Feeding
- Candling
- Sorting of files
Friday (February 03, 2023)
- Feeding
- Slaughtering
- Collecting of Eggs
- Inventory
- Cutting of the Feather
WEEK 3
Monday (February 06, 2023)
- Loading of Eggs
- Slaughtering
- Collecting of eggs
Tuesday (February 07, 2023)
- Feeding
- Collecting of eggs
- Slaughtering

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- Feeding
- Collection of eggs

- Washing and refilling of the water container mixed with electrolytes
- Vaccination
- Tagging
- Mixing of Feed
- Feeding
- Candling
- Seminar
OTHER ACTIVITIES ACCOMPLISHED
 Data Recording
 Mortality rate up to hardened chicks is 10%
 Recording daily number of eggs laid by each family
 Artificial insemination
 0.5 ml of semen
 When the rooster's semen supply is insufficient, simply add dextrose to fill the
shortage.
 Forage fermentation
 Swamp cabbage 30 kg
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 Molasses 3litres
 Cleaning management and Maintenance
Collected Semen
Gathered data (record the color of

the semen, texture, pen number and
measurement of the semen)
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Insemination of the hen with semen Chopping of swamp cabbage
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Mixing of EMAS to the chopped swamp cabbage
PRACTICES AND MANAGEMENT
Collection of eggs
The collection of eggs will last to 7 days, and eggs were labelled families 1,2,3,4 and FB
which stands for Flock Breeding.
Collection of
eggs
Cleaning management of eggs
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Recorded data (date today and family)
INCUBATING
The main goal of incubation is to increase the hatching rate and produce healthy
hatched birds. Incubation temperature is not only the most important factor in the
hatching process, but is additionally related to the growth performance of chicks after
hatching. The eggs will be incubated for 11 days, and in the 7th day candling process
takes place. In this process unfertile and fertile eggs will be identified. The fertile eggs
will be bought back to the incubator after the candling process.
Recorded
data (date today, Loading of eggs in the incubator
family, date of Candling, and
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date to transfer in hatchery incubator).
HATCHING
The eggs will be transported to the hatchery following the incubation stage and
will remain there for 3-5 days.
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Sorting of eggs by family (Fb, F1,F2,F3, and

F4)
Transporting of 67 eggs then
brought to the hatchery incubator
Loading activity of 67 pcs. eggs
at the hatchery incubator.
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BROODING
Day-old chicks are tagged using the slitting technique following hatching day, before
being moved to the brooding area. Brooding area is where the day-old chicks were kept to
provide artificial heat. Day old chicks will stay in the brooding area for 21 days. The New Castle
Disease Vaccine will be administered to brooder chicks prior to transfer to the hardening area.
Slitting
process for identification Recorded data (date and brooder’s family)
of brooder’s family.
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Transferred 52 heads of brooder chicks at brooding unit
HARDENING
Chicks from brooders will be moved into the hardening area and will receive
electrolyte supplements and artificial light support. For 45 days, brooder chicks will
remain in the hardened area.
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Transferred to harden stage/area
GROWING
The Hardened chicks will be moved to grower pens at the 45th day, where they
will be exposed to various weather conditions. The grower hens take several days to
properly acclimate to their new environment, before releasing hardened chicks into
grower enclosures and to prevent mortality, workers will assess the weather. At this
point, it is simple to define the pure breed zampen, which will be chosen for breeding
while the off-type zampen will be for slaughtering purposes.
Grower chickens
BREEDER
Selected chickens were being cared for breeding purposes; the peak egg-laying
period for chickens lasts between six to twelve months.
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FEEDING STAGES OF ZAMPEN NATIVE CHICKEN
Brooding Stage Hardening Stage Grower stage Breeder stage
Commercial feeds Commercial feeds/ Commercial feeds/ Breeder

Fermented feeds Fermented feeds feeds/Layer feeds/
Fermented feeds
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Feeds Formulation for daily production
Commercial feeds/ Coco Dregs 50%

Corn bran 25%
Forages 25%
Total 100%
Forages to Molasses ratio
Forages Formulation
Tricanthera 10kg:1litre of molasses
Kangkong 10kg:1litre of molasses
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CHAPTER I
B. BANANA LAKATAN TISSUE CULTURE

I. INTORDUCTION
Background of the study
Tissue culture is the cultivation of plant cells, tissues or organs on specially formulated
nutrient media. Under the right condition the entire plant can be regenerated from a single cell or
tissue. Tissue Culture often called micro propagation is seen as important technology for
developing countries for the rapid production of quality planting materials. Unfortunately, tissue
culture is labor intensive, time consuming, and can be very costly.
The tissue culture center propagated banana (lakatan) through the use of chemicals
(medium) and banana tissue. The project was developed to rapidly reproduce a uniform or a
characteristic of preferred species of banana. The intern students were assigned in the tissue
culture center to expose them in different laboratory skills and acquire experiences about plant
propagation with technology.
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The Underlying principles in plant tissue culture are simple:
a) It is necessary that the plant came from the intact plant.

b) It is necessary that the plant part be provided with the appropriate environment.
c) This can be done by providing suitable culture medium and proper culture
condition.
d) Finally, all the above activities should be done under aseptic condition.
The importance of tissue culture
a) Rapidity with which a selected species may be generated easy and economical.
b) Production of many uniform plants produced in the short period of time.
c) Characteristic of preferred species maintain the generic make-up and
morphological
d) uniformity of plants derived from the cultivar from generation to generation.
Requirements for tissue culture technology
a) Aseptic and excellent handling techniques

b) Explants source
c) Manipulated environment
-Culture Medium
-Culture incubation (temperature, light duration).
Chemical requirements of Tissue culture media
a. Macro and Micronutrients

b. Carbon and energy source
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c. Vitamins
d. Growth regulators (plant hormones)
e. Solidifying agents
f. Ph (potential hydrogen)
g. Stock solutions
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CHAPTER II
II. ACTUAL UNDERTAKINGS/ ACTUAL ACCOMPLISHMENTS

Preparation of stock solutions (1 Liter)
a. MS I 250 ml
o Potassium nitrate - 11.875
o Ammonium nitrate - 10.3125
o Calcium nitrate - 2.75
o Magnesium sulfate - 2.3125
o Potassium phosphate - 1.0625
b. MS II-250 ml
o Manganese sulfate – 2.230
o Boric acid - 0.620
o Potassium iodine – 0,083
o Zinc sulfate – 0.860
o Sodium molybdate – 0.025
o Copper sulfate – 0.025
o Cobalt chloride – 0.0025
c. MS III 250 ml
o Glycine - 0.05
o Thiamine - 0.0025
o Pyridoxine - 0.0125
o Nicotinic Acid - 0.0125
o Myo- Inositol - 2.5
d. BAP 250 ml - dissolved in 5ml sodium hydroxide
o Sucrose 50g
o Agar 16
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Stock solution namely: MSI, MSII and MSIII
1. Assemble all glassware: beaker, spatula, aluminum foil, volumetric flask, funnel, stirring
2 Check the digital balance and arrange the chemical to be used.
3. Label the beakers according to the intended use.
4. Weigh the specified amount of each of the chemicals listed in the table.
5 Dissolve the chemicals separately in minimum volumes of distilled water either by
stirring manually or in magnetic stirrer, until dissolve.
6 Pour the fully dissolve content of each beaker in to 1.000 ml volumetric flask, using glass
funnel, consequently rinse beaker with distilled water and add the rinse into the
volumetric flask. Dilute the resulting solution to the final volume of 1 liter
7. Mixed the solution thoroughly with magnetic stirrer or agitating the last before
transferring to properly dark bottle
8. Label the bottle with the name and concentration of stock solution and date of
preparation.
9. Refrigerate the stock solution.
Note: Stock solutions are only good for 6 months
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Preparation of culture media (Proliferation media)
A. Prepare and align all stock solution needed (MS I, MS II, MS III,
iron chelate and growth regulators).
B. Weigh 20g sugar and dissolve in distilled water. Weigh 7g agar

C. Measure the following stock solution add each measured stock solutions with the used of
graduated cylinder rinse graduated cylinder with distilled water and pour it into 500ml.
D. Melt the agar using microwave oven melting time in a microwave oven depends on the
amount of agar volume of the medium.
E. Mix the melted agar with the prepared stock solution and bring into the final volume of 1
liter. Measure the pH of the medium and adjust the pH level to 6.0.
F. After getting the pH, dispense the medium into suitable, autoclavable culture media like
packing jars.
G. Sterilize the dispensed media in the autoclave for 15 minutes at 15 Psi.
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MATERIALS NEEDED
Materials Image Description

Digital weighing scale Digital weighing scales
use electronic sensors to
measure weight and display it
on a screen
Laboratory gown
Prevent contamination from
spreading outside the lab
(provided they are not worn
outside the lab)
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Magnetic capsule Stir bar is placed inside a

container of liquid or solution
that is seated on a magnetic
stirrer, creating a rotating
magnetic field using a rotating
magnet or multiple stationary
electromagnets
Denatured alcohol
Denatured alcohol is used as a
solvent and as fuel for alcohol
burners.
Magnetic stirrer
Magnetic stirrers are essential
equipment you should get for
your laboratory equipment. It is
used for bending two sample
components in biological or
chemical experiments. This device
can be used for liquids or solids to
get a consistent mixture.
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Scalpel/Blade
Used to cuts and properly
sliced to prevent the corm from
being damaged.
Forceps Laboratory tweezers and

forceps are small tools that
can be used to grab and
manipulate small or fragile
objects in a variety of lab
and other working contexts.
Pitcher
It is a plastic container with
spout used for storing and
pouring liquids.
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Petri dish
A scientist uses a shallow clear
covered dish to hold growth
liquid in which cells can be
cultivated. Originally, bacteria,
fungus, and microscopic
mosses’ cells.
Alcohol lamp In a laboratory, an alcohol

lamp is use for heating,
sterilizing, and combustion.
The fuel for the alcohol lamp
is the denatured alcohol.
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Graduated cylinder Graduated cylinders are

long, narrow tubes that are
used to measure liquid
volume. They are not meant
for stirring, heating, or
weighing. Graduated
cylinders are typically 5ml to
500 ml in size.
Markers Marker/pens are used to create
highly visible (and occasionally
permanent) ink or paint lines
on practically any work surface
or material.
Trays
It is used for carrying, storing,

or displaying all kinds of
things.
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Funnel
Liquids or fine-grained
substances (powders) are
funneled into labware with a
small neck or opening using
laboratory funnels.
Erlenmeyer flask Erlenmeyer flasks are used to

contain liquids and for mixing,
heating, cooling, incubation,
filtration, storage, and other
liquid-handling processes.
Their slanted sides and narrow
necks allow the contents to be
mixed by swirling without the
risk of spills, which is useful
for titrations and for boiling
liquids.
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Beaker A beaker is a cylindrical glass

or plastic vessel used
for holding liquids. It is a
multipurpose piece of
equipment used for containing
a chemical reaction, measuring
liquids, heating them over a
Bunsen burner's flame or
collecting them in a titration
experiment.
Bottles bottle, narrow-necked, rigid or
semirigid container that is
primarily used to hold liquids
and semi- liquids. It usually has
a close-fitting stopper or cap to
protect the contents from spills,
evaporation, or contact with
foreign substances.
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Stirring rod A glass stirring rod, glass rod,

stirring rod or stir rod is a piece
of laboratory equipment used
to mix chemicals and liquids
for laboratory purposes. They
are usually made of solid glass,
about the thickness and slightly
longer than a drinking straw,
with rounded ends.
Spoon Spoon is handy for mixing,
scraping, and handling
powders. An everyday one-
piece tool for use in the lab or
field.
Tissue paper Tissue Paper is a multipurpose

paper that is ideal for mopping
up liquids and hand wiping.
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Aluminum Foil
It is usually lacquered or water-
based latex-coated, or
laminated to a polymer film by
adhesive or extrusion coating
or lamination.
Sprayer Sprayer are also utilized to

apply substances to the
manufacturing and production
lines, such as performance
materials and pest controls.
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Bolo Bolo is used for cutting weeds

and chopping branches of the
suckers.
Digging bar A digging bar is a long, straight

metal bar used for various
purposes, including as a post
hole digger, to break up or
loosen hard or compacted
materials such as soil, rock,
concrete and ice or as a lever to
move objects.
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PRODUCTION FLOW OF TISSUE CULTURE BANANA LAKATAN
Cleaning of
bottles and lids Sterilizing the jar Establishing
at 15 psi Initial culture
Transferring of
Preparation of
culture media in Incubation
stock solution
the bottles
Collection of Preparation of
sucker culture media
Cleaning of Washing of
sucker sucker
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PRACTICES AND MANAGEMENT
The interns collected suckers from the banana plantation of WMSU AGRI. by digging
the soil around the sucker, and carefully detaching the sucker from the mother plant to
avoid cracking the corm of the sucker.
After collecting the suckers, they washed it with running water to remove any adhering
dirt and contamination.
They trimmed the leaf bracts and corm tissue up to the size sufficient enough to protect
the growing point from bleaching.
The trimmed corm was washed with soap solution.
Corm was disinfected by soaking it in formalin for 15minutes
Disinfectant was decanted and corm was rinsed three times with distilled water. The
disinfected corm was the transferred to the inoculation chamber for initial culture.
Establishing initial culture
 In the culture room, the interns further excised the corm. Making sure that the
cube still
Contained the shoot apex.
 The corm was divided longitudinally into four parts having the shoot apex in
each section and inoculated in proliferation media.
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Nursery Management
When the white cord roots have developed and the plantlets have 3-4 expanded leaves
and attained a minimum height of three to four inches, they are ready for potting out.
Acclimatization
Selected cultured banana with roots were then placed outside of the laboratory to
introduce a semi-natural-laboratory environment for at least one day and further

transplanted in
the acclimatization area to acclimatize the plantlets into a new environment before
transplanting it in the nursery.
Weeding and Watering
The seedlings in the nursery had their dead leaves and weeds pulled. In order to
obliterate possible nutritional rivals. Once daily, either in the morning or in the
late afternoon or early morning
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ACTUAL ACTIVITIES ACCOMPLISHED
This table shows the list of the daily activities the interns had undertaken at Tissue culture
Banana Lakatan.

Cleaning management (sweeping and mopping)
Washed bottles and lids (292 pcs.)

Cleaning management (Sweeping and mopping)
Media preparation (Media solution 3)
Weighed each the solutions using digital weighing scale
Using a magnetic stirrer, stirred the liquid thoroughly.
Stored the mixtures in the refrigerator
Cleaned and sanitized the storage area.
Piled the bottles (medium)
DE leafing
Watered the banana lakatan
Gathered the solutions to make a media
o MS I 80ml
o MS II 20ml
o MSIII 20ml
o BAP 60ml
o IAA 20ml
o Sucrose 50g
o Agar 12g
Mixed the stock solutions
Checked the pH level
Cooked the Agar
Put the media in the bottles
Labelled the bottles
Sterilized the bottles
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Monday (February 27, 2023)

Acclimatized the plantlets and covered with bottles to maximize the temperature because the
plantlets are still in adjusting of the new environment.
Washed the plantlets of a running water to remove the remaining cultured media of the
germinated Banana Lakatan.
Applied fungicide to the banana lakatan plants and soaked the plantlets before to transplant.
Gathered Banana Lakatan suckers (12 pcs.)
Washed and rinsed the suckers then cut the stem.
Tuesday (February 28, 2023)
Cleaning and disinfection of a corms.
Setting up and sanitized the inoculation chamber (Laminar)
Inoculated 12 pieces of corms
Total inoculated is 24 pieces
Wednesday (March 1,2023)
Orientation of the Tissue culture in Banana Lakatan production
OTHER ACTIVITIES
 Cleaning management - sweeping inside and out of the tissue culture building and mopping
the floor.
 Planting of banana lakatan – 4 pieces planted Banana Lakatan
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CHAPTER III
PROBLEMS ENCOUNTERED AND SOLUTION UNDERTAKEN
 Problems Encountered
1. Zampeen Native Chicken - IGP
 Lack of feeds supply
 Unavailability of Disposal area for Dead chicks
 Damaged pens due to bad weather conditions
 Waste disposal
 No proper area for slaughtering
 Solution Undertaken
 Mixing of forages (trichantera, coco dregs, malunggay, oregano etc)
 The interns proposed to make the septic tank a disposal area for dead birds
 Pens were fixed and changed the damaged materials with stronger ones.
2. BANANA LAKATAN TISSUE CULTURE
 Problems encountered
 Lack of laborers that can speed up the production
 Some of the bottles were not totally cleaned and rinsed
 Contamination of media
 Solution Undertaken
 The interns rewashed the bottles to ensure that there would be no contamination
 The contaminated media was removed immediately
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CHAPTER IV
CONCLUSION
The Bachelor of science in Agricultural Business of Western Mindanao State University

interns had accomplished the goals of their practicum in campus during the second semester of
the school year 2022-2023.
The interns successfully conducted their practicum internship, and they were able to learn
the feed formulation, feed fermentation, feeding management, artificial insemination, care, and
maintenance in every area. In the Tissue Culture Banana Lakatan production, they were able to
perform media preparation, stock solution, and establish initial culture of the Banana Lakatan, in
which they were to produced 24 pieces of the inoculated banana corms.
Furthermore, the Bachelor of Science in Agricultural Business interns conclude that thee
entire experienced and exposure of the team has helped to improved each one’s character and
personality and was able to become better through the entire process in the different areas. The
group has achieved their goals easily through motivating each other and has a unity to solved
the problems encountered.
51
RECOMMENDATION
Zampen Native Chicken - IGP
The proponents recommended creating a space for seclusion and waste disposal. As a
result, the surroundings become cleaner and the mortality rate of the chickens will be reduced.
Pen houses should be expanded for the chickens to have enough space and prevent stampede.
Tissue culture Banana Lakatan
The staff needs an additional employee to easily done the activities in producing Banana
lakatan and to produces mass production of Banana Lakatan.
52
CHAPTER V
LEARNING EXPERIENCE
The fourth- year Bachelor of science in Agricultural Business of Western Mindanao State
University interns were able to conduct their practicum and were exposed to various research-
based project at Western Mindanao State University; Zampen Native Chicken – IGP, and Tissue
culture Banana Lakatan.
The interns were able to experienced and Learned from the different areas/activities in
Zampen Native Chicken – IGP. Just like feeds fermentation and formulation, Artificial
insemination, slitting, tagging, vaccination, eggs collection, labeling, incubating, candling,
deworming, slaughtering and transferring of chickens to the next growth stage. In the Tissue
culture, the interns also able to experienced collect suckers, acclimatizing, mixing of chemicals
to make a stock solution, media preparation and inoculate corms in the bottles which was has a
media.
53
APPENDICES
APPENDIX A
DEFINITION OF TERMS
Artificial Insemination (AI) – is a method and process in which the sperm was
manually and directly delivered into the female vagina in hope of getting fertilized.
Breeder – intended for breeding or reproducing.
Brooding – the period after hatching of eggs where special care and attention must be
given to ensure the health and survived.
Candling – the process of holding strong light below and above the eggs to observe the
embryo in search of signs of life or development.ee
Coco dregs – is a dried coconut.
Corm – rounded thick modified underground stem base acting as a vegetative

reproductive structure where the banana plant produces suckers.
Culture media – a growth media that could be solid, liquid, and semi-solid created to
support growth.
E-MAS or effective Microorganisms Activated solution – is a fertilizer that elicits the

growth of microbial benefits.
Feeds Fermentation – is the process of fermenting feeds.
54
Feed Formulation – is the process of determining the volumes of ingredients and

additives to blend to create compound feeds that meets up the target nutrient
requirements of a species and achieve the goals of production at an optimized cost.
Grower chickens – are chicken allotted to be given away to farmers who are interested
in growing of chickens.
Hardening – is the stage that allows the chick ti adapt to their surroundings and
environment to become stronger before letting loose in the range.
Hardened – the stage that chickens had fully adapted to their surroundings,
environment, and increasing their level of survival.
Incubator – a device or insulated enclosure used for the hatching of fertile eggs which
regulates environmental conditions such as temperature and humidity in optional level.
Initial culture – the first step in the process of plant propagation in the tissue culture.
Laminar Flow – a laminar flow cabinet or tissue culture hood is a carefully enclosed
bench designed to prevent contamination.
Molasses – is a substance resulting from refining sugarcane or a bi-product.
Proliferation – a single isolated cell that divides and rapidly reproduce which gives rise
to a new plant.
Rooting – a type of culture media used for the multiplication of plants.
Stock solution – is composed of different solid solution, each weigh out in an

appropriate amount diluted in lower concentration such as distilled water.
55
Sucker – a plant growth that originated and grows from the rootstock of the mother
plant.
Tissue Culture – also called as micro propagation is a technology for cultivation of

plant cells, tissues, or organs for rapid production of quality planting material.
Vitro – a living-materials culture.
REFFERENCES
 Internship Practicum Manuscript of : QUEVAS, ARKQUIZ P.

PARADERO, KAREN KAYE A.
SUCLOM, LHEA M.
 Internship Practicum Manuscript of : FRANCISCO, LILETTE T.
MACROHON, ALBERT JR.
FRANCISCO, JHON KRIS D.
56
APPENDIX B
AUTHOR BIOGRAPHY
DIVINE GRACE N. MANALO
Centro Limaong, Zamboanga City
Philippines 7000
+63 9050636462
Gt201900497@wmsu.edu.ph
1. PERSONAL INFORMATION
Date of Birth: September 25, 2000
Place of Birth: Matiti, Zamboanga City
Age: 22
Religious Affiliation: Roman Catholic
Ethnicity: Chavacano
Language Spoken: Chavacano
57
Civil Status: Single
II. EDUCATIONAL BACKGROUND
TERTIARY: Western Mindanao State University
San Ramon Zamboanga City
2019 – Present
COURSE: Bachelor of Science in Agricultural Business
SENIOR HIGH SCHOOL: Limaong National High School
Limaong, Zamboanga City
2016-2018
COURSE: General Academic Strand
HIGH SCHOOL: Liamaong National High School
2012 – 2016
ELEMENTARY: Limaong Elementary School
58
BABIEGRACE M. AYUNTING
Purok 4 Sinubong, Zamboanga City
Philippines 7000
+63 9260993261
Date of Birth: February 8, 1999
Place of Birth: Tulungatung, Zamboanga City
Age: 24
Ethnicity: Visayan
Language Spoken: Visaya
59
2019 – Present
SENIOR HIGH SCHOOL: COMTECH INSTITUTE
Baliwasan chico, Zamboanga City
2016-2018
COURSE: Home Economics
HIGH SCHOOL: Ayala National High School
Zone 6 Ayala, Zamboanga City
2012 – 2016
ELEMENTARY: Tulungatung Elementary School
Tulungatung Zamboanga City
60
RAPHAEL LANCE T. GOMEZ
Sinunuc, Zamboanga City
Philippines 7000
Date of Birth: February 8, 1999
Place of Birth: Tulungatung, Zamboanga City
Age: 24
Ethnicity: Visayan
Language Spoken: Visaya
61
2019 – Present
SENIOR HIGH SCHOOL: COMTECH INSTITUTE
Baliwasan chico, Zamboanga City
2016-2018
COURSE: Home Economics
HIGH SCHOOL: Ayala National High School
Zone 6 Ayala, Zamboanga City
2012 – 2016
ELEMENTARY: Tulungatung Elementary School
Tulungatung Zamboanga City
62

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WESTERN MINDANAO STATE UNIVERSITY

INTERNSHIP-PRACTICUM AT VARIOUS RESEARCH-BASED

Presented to the Faculty of the Agribusiness Department

GOMEZ, RAPHAEL LANCE T.

In partial fulfillment of the requirement for the Degree of Bachelor of Science in

Agribusiness entitled: “Practicum Internship on Various Researched-Based Projects in

Babiegrace M. Ayunting. Is hereby recommended for oral defense.

MS. MERCELYN B. TILUS MRS. EMELYN D. VARONA

In partial fulfillment of the requirement for the Degree of Bachelor of Science in

MRS. GLADESS P. PAGAL DR. ELDERICO P. TABAL

Signed this ___ day of _______ in the year ________ at _________________________

Signature with name

objective is to expose students to different Agri-entrepreneur applying the theories,

techniques, philosophies and skills learned from various subjects.

mushroom production, Food processing and market the available products.

Grateful acknowledgement is hereby given to the following individuals for their

suggestions and correction to further improve of this study.

continue strive in our studies particularly in pursuing BS Agribusiness.

during the rough moments in conducting this study.

The BSAB (Bachelor of science in Agricultural business) 4 th students of Western

Mindanao State University- College have conducted their practicum in campus.

feed formulation mixing, vaccination of chicken, deworming of chicken, slitting,

defeathering, and slaughtering of chickens.

different Tissue culture Laboratory areas.

Significance of the study

The study served as a resource, a manual, and a contribution to the students'

1. To be able to learn and manage the following researched-based projects

a. Zampen Native Chicken (Income Generating Project)

2. To identify the challenges and problems of the different areas assigned.

II. ACTUAL UNDERTAKINGS/ ACTUAL ACCOMPLISHMENTS

A. ZAMPEN NATIVE CHICKEN (Income Generating Project)

Growth and Developmental Stages of Zampen Native Chicken-IGP

Figure 1. Growth and Developmental Stages of Zampen Native Chicken-IGP

Note: 3 years old breeder considered as Culled

Zampen Native Chicken (Income Generating Project)

ACTUAL ACTIVITIES ACCOMPLISHED

Tuesday (January 24, 2023)

Wednesday (January 25, 2023)

- Vitamins and antibacterial/anti-infective

Thursday (January 26, 2023)

Monday (January 30, 2023)

Wednesday (February 08, 2023)

Thursday (February 09, 2023)

OTHER ACTIVITIES ACCOMPLISHED

Gathered data (record the color of

Insemination of the hen with semen Chopping of swamp cabbage

Mixing of EMAS to the chopped swamp cabbage

PRACTICES AND MANAGEMENT

Cleaning management of eggs

Recorded data (date today and family)

family, date of Candling, and

date to transfer in hatchery incubator).

Sorting of eggs by family (Fb, F1,F2,F3, and

Transporting of 67 eggs then

brought to the hatchery incubator

Loading activity of 67 pcs. eggs

at the hatchery incubator.

Transferred 52 heads of brooder chicks at brooding unit

Transferred to harden stage/area

FEEDING STAGES OF ZAMPEN NATIVE CHICKEN

Brooding Stage Hardening Stage Grower stage Breeder stage

Commercial feeds Commercial feeds/ Commercial feeds/ Breeder

Signed this _ day of _ in the year at _____________________