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  • Chromatography Qa

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    Zunaira Jahanzaib
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    Chromatography is a technique used to separate mixtures and identify their components. The document discusses using chromatography to analyze amino acid samples, with a diagram showing the migration of amino acids on a paper chromatogram sprayed with ninhydrin. Sample C contained more than one amino acid based on its spread spot. The origin line is drawn in pencil so the solvent front can be marked, and spraying with ninhydrin allows visualization of the separated amino acids.

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    Chromatography Qa (2) (1)

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    Chromatography is a technique used to separate mixtures and identify their components. The document discusses using chromatography to analyze amino acid samples, with a diagram showing the migration of amino acids on a paper chromatogram sprayed with ninhydrin. Sample C contained more than one amino acid based on its spread spot. The origin line is drawn in pencil so the solvent front can be marked, and spraying with ninhydrin allows visualization of the separated amino acids.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    7 views1 page

    Chromatography Qa

    Uploaded by

    Zunaira Jahanzaib
    Chromatography is a technique used to separate mixtures and identify their components. The document discusses using chromatography to analyze amino acid samples, with a diagram showing the migration of amino acids on a paper chromatogram sprayed with ninhydrin. Sample C contained more than one amino acid based on its spread spot. The origin line is drawn in pencil so the solvent front can be marked, and spraying with ninhydrin allows visualization of the separated amino acids.

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    © All Rights Reserved
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    of 1

    WORK SHEET

    CHEMISTRY CLASS:___

    NAME: __________________ DATE: ______________

    CHROMATOGRAPHY

    1. Define chromatography.

    ____________________________________________________________________________________
    _________________________________________________________________________________ [1]
    2. Chromatography can be used to identify amino acids from a sample protein. The diagram shows
    a chromatogram obtained when three samples of amino acids were analyzed .The paper was
    sprayed with ninhydrin.

    a) Why is the origin line drawn in pencil?


    _______________________________________________________________________________ [1]
    b) Which amino acids could possibly be the same?
    _______________________________________________________________________________ [1]
    c) Which amino acid sample contains more than one amino acid?
    Sample ________________________________________________________________________ [1]
    Explanation ____________________________________________________________________ [1]
    c) Suggest why it is necessary to spray this chromatogram with a locating agent?
    _______________________________________________________________________________ [1]
    d) Define Rf and calculate the Rf values of sample A,B,C and D.
    __________________________________________________________________________________
    __________________________________________________________________________________
    __________________________________________________________________________________
    __________________________________________________________________________________
    __________________________________________________________________________________
    __________________________________________________________________________________
    __________________________________________________________________________________
    __________________________________________________________________________________
    _______________________________________________________________________________ [4]

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