INDEX

1. CERTIFICATION

2. ACKNOWLEDGEMENT

3. INTRODUCTION

4. AIM OF THE EXPERIMENT

5. APPARATUS REQUIRED

6. PROCEDURE

7. RESULT

8. CONCLUSION

9. BIBLIOGRAPHY
 CERTIFICATION

This is to certify that P.L. Sameeksha a student of class 11-M has

successfully completed the project on the topic “Checking the bacterial

contamination in drinking water by testing sulphide ion” under the

guidance of Ms. Vaidegi Prbhu.

 ACKNOWLEDGEMENT

I take this opportunity to express my gratitude towards my chemistry

teacher Ms.Vaidegi Prabhu, for her precious and valuable guidance which

played a crucial role in not only any investigatory project on the topic

“Checking the bacterial contamination in drinking water by testing sulphide

ion”, but also throughout the session with my studies. I am very grateful to

the school as well, for giving me this opportunity.

Last but not the least I would like to thank my parents and friends for

helping me in this project.

 INTRODUCTION

The sulphide ion test for bacterial contamination is good. In surface

waters, hydrogen sulphide is formed under oxygen deficient conditions.
Hydrogen sulphide is also produced from the decomposition of sulphur
containing organic compounds. The concentration of sulphide ion becomes
significant only at pH 10 or above. Under acidic conditions, the
concentration of hydrogen sulphide predominates. Hydrogen sulphide is a
weak acid, which ionizes to yield hydro sulphide (HS-) and sulphide (S2)
ions. Hydrogen sulphide is highly toxic to fish.
Concentrations of total sulphide as low as 0.01 mg/L make the water
uninhabited fish culture. Hydrogen sulphide also reduces the aesthetic value
of the water body due to foul colour. The bacterial contamination can be
tested using H2S strip. Presence of pathogenic bacteria in water can be
detected by testing the sulphide content of water. The presence of sulphide
ions in water is an indicator of :
I. High PH above 10
II. Sulphide producing bacteria
III. Diminished oxygen concentrations conducting the sulphide ion test.
The sulphide ion test is conducted using an hydrogen sulphide strip
(H2S). the H2S strip is dipped into the water to be tested for 14 to 16
hours. The H2S strip turns black if sulphide ions are present in the
water. The black colour is due to the ionization of the H2S.
 AIM

To check the bacterial contamination in drinking water by testing sulphide

ion.

APPARATIES REQUIRED

1. Hydrogen Sulphide (H2S strip)

2. Beaker

3. Drinking water

4. Thermometer

Chemical Required

1. Hydrogen sulphide (H2S)

 PROCEDURE

This experiment is to be done in an incubator

I. Add about 250 ml of water in a breaker.

II. Dip the H2S strip in the beaker with water.

III. Heat the beaker from about 35 degree centigrade.

IV. Check the temperature

V. Keep it in incubator for 14-16 hour of time.

VI. Check the result if the water turns black then it is unfit for drinking

highly contaminated for drinking or human use.

VII. The sulphide ion test for bacterial contamination is good. In

surface waters, hydrogen sulphide is formed under oxygen

deficient conditions. Hydrogen sulphide is also produced from the

decomposition of sulphur containing organic compounds. The

concentration of sulphide ion becomes significant only at pH10 or

above.
 OBSERVATION

Source of water Result

Water from the tap of house at Water is fit for drinking

madanrting

Water from the tap of happy Water is unfit for drinking

valley houses

Spring water Water is fat for drinking

River water Water is not fit for drinking

 RESULT

By the experiment done, the result says that at some place water is fit for

drinking at some places whereas it is unfit for drinking at some places. Especially

water is unfit for drinking from river bodies.

CONCLUSION

 Water is mostly unfit for drinking from river.

 Water is also unfit for drinking at some of the water that come to houses.

 Therefore we should purify the water bodies before drinking. And we

should not drink water from random places.

 BIBLIOGRAPHY

 Chemistry lab manual class 11th

 Chemistry NCERT book

 www.brainly.in

 www.indiawater.org

 www.merintation.com

 www.coursehero.com