Kendriya Vidyalaya No.

4
Central University Tezpur
P/O: Napaam, Dist: Sonitpur, Assam, Pin: 784028

English
Investigatory
Project
Topic:
Submitted by: Submitted to:

Bishal Nath
Naveen Khimta
ACKN I wish to express my deep gratitude

OWLE and sincere thanks to the Principal

Mr. Diganta Kumar Sharma, KV
Central University Tezpur, Assam
DGEM for his encouragement and for all the
facilities that he provided in this
ENT project work. I sincerely appreciate
this magnanimity by taking me into
his fold for which I shall herein indebted to him.

I extent my hearty thanks to Mr. Naveen Khimta,

Chemistry teacher, who guided me to the successful
completion of this project. I take this opportunity to
express my deep sense of gratitude for his invaluable
guidance, attitude and immense motivation, which has
sustained my efforts at all stage of this project work.

1
 CERT
IFICA
This is to certify that
satisfactorily
TE Bishal Nath has
completed the
project in chemistry on “To check the bacterial contamination
of drinking water by testing sulphide ion” prescribed by the
CBSE course in the school in the year 2022-23. I have examined
the project and hereby accord by my approval of it. As a study
carried out and presented in the manner required for its
acceptance. This does not necessarily endorse or accept every
statement made, opinion expressed, or conclusion drawn. It only
signifies the acceptance of the project for which it is submitted.

2
 INDEX
SL. NO. CONTENT PAGE
NO.
1 INTRODUCTION 4

2 AIM 5

3 MATERIALS REQUIRED 6

4 PROCEDURE 7

5 OBSERVATION 8

6 RESULT 9

7 CONCLUSION 10

INTR
3

ODUC
 TION

The sulphide ion test for bacterial contamination is good. In surface

water, hydrogen sulphide is formed under oxygen deficient
conditions. Hydrogen sulphide is also produced from the
decomposition of Sulphur containing organic compounds. The
concentration of sulphide ion becomes significant only at pH 10 or
above.
Under acidic conditions, the concentration of hydrogen sulphide
predominates. Hydrogen sulphide is a weak acid, which ionizes to
yield hydrosulphide (HS) and sulphide (S) ions. Hydrogen sulphide is
highly toxic to fish. Concentration of total sulphide as low as
0.01mg/l make the water uninhabited fish culture. Hydrogen
sulphide also reduces the aesthetic value of the water body due to
foul colour. The bacterial contamination can be tested using H 2S
strip.
Presence of pathogenic bacteria in water can be detected by testing
the sulphide content of water. The presence of sulphide ions in
water is an indicator of:
i) High pH above 10
ii) Sulphide producing bacteria
iii) Diminished oxygen concentration.
Conducting the sulphide ion test: The sulphide ion test is conducted
using a hydrogen sulphide strip (H2S). The H2S strip is dipped into the
water to be tested for 14-16 hours. The H2S strip turns black if
sulphide ions are present in the water. The black colour is due to the
ionization of the H2S.

AIM 4
 To check the bacterial contamination in drinking
water by testing sulphide ion.

5
 REQU
IREM
 Apparatus ENTS required:
I. Hydrogen sulphide (H2S strip)
II. Beaker
III. Drinking water
IV. Thermometer
 Chemical required:
I. Hydrogen sulphide (H2S)

6
 PRO
The experiment
CED is to be done in
an incubator.
1) Add about
URE 250ml of water in
a beaker.
2) Dip the H2S strip in the beaker with water.
3) Heat the beaker for about 35 degrees centigrade.
4) Check the temperature (temperature should be at
35 degrees centigrade).
5) Keep it in an incubator for 14-16 hours of time.
6) Check the results if the water turns black then it is
unfit for drinking / highly contaminated for
drinking or for human use.

7
 OBSE
RVAT
ION RESULT
SOURCE OF WATER

Tap water Fit

River water Unfit

8
 RESU
By the experiment LT done, the result
says that at some places water is fit
for drinking whereas it is unfit for drinking at some other
places especially water is unfit for drinking water from river
bodies.

9
 CONC
Water is mostly
LUSI unfit for drinking
from river.
should always
ON Therefore, we
purify the water
before drinking and we should not drink water from random
places.

10
Thank
You
11