PPRQLD

)RU DPPRQLD GHWHUPLQDWLRQ LQ SODVPD

PRO VLV H WULI H L D FDSSHG W EH DV VRR DV SRVVLEOH

,W KDV EHH VKR WKDW L FUHDVHG OHYHOV RI DPPR LD K SHU E GGLWLYHV use only EDTA ( QWLFRDJ ODQWH : IURP :LH
ammonemia) produce toxic e ects on the central nervous HU ODE DV D WLFRD OD W
system. There are several diseases, both inherited and F .QRZQ LQWHUIHULQJ V EVWDQFHV YLVLEOH KHPRO VLV L WHU
acquired, causing hyperammonemia. Inherited de ciency feres with the assay. No signi cant interference was observed
of the enzyme of the urea cycle is the leading cause of E ELOLU EL S WR P G/ KHPR OREL S WR P G/ RU
K SHUDPPR HPLD L FKLOGUH PR WKH DF LUHG FD VHV WUL O FHULGHV S WR P G/ 5HIHU WR R ELEOLR UDSK
WKDW SURG FH D L FUHDVH L DPPR LD OHYHOV DUH DGYD FHG for the e ects of drugs in this method.
OLYHU GLVHDVH D G NLG H IDLO UH G WDELOLW DQG VWRUDJH LQVWU FWLRQV SODFH WKH VDPSOH L
ice and test immediately. The plasma is stable for 3 hours at
3 3/( R
L D FORVHG FR WDL HU H WULI H WKH VDPSOHV FR WDL L
The method is based on the reaction of ammonia in the SUHFLSLWDWH EHIRUH SHUIRUPL WKH DVVD
VDPSOH LWK α NHWR O WDUDWH D G 1 3+ L WKH SUHVH FH RI
O WDPDWH GHK GUR H DVH / + WR LHOG O WDPDWH D G ( ( ( / R SURYLGHG
1 3 . The decrease in absorbance at 340 nm is propor 0LFURSLSHWWHV D G SLSHWWHV IRU PHDV UL WKH VWDWHG YRO PHV
WLR DO WR WKH DPPR LD FR FH WUDWLR L SODVPD 6SHFWURSKRWRPHWHU
/ + :DWHU EDWK DW WKH VHOHFWHG UHDFWLR WHPSHUDW UH
α NHWR O WDUDWH 1+ 1 3+ α O WDPDWH 1 3 6WRS DWFK

3 ( ( (
HDJHQW PPRO / α NHWR O WDUDWH PPRO / :DYHOH WK P
NADPH and 1000 kU/L GLDH in 100 mmol/L Tris bu er, pH 8.6. Reaction temperature: 25, 30 or 37R
5HDFWLR WLPH PL WHV
3 ( ( ( 6DPSOH YRO PH /
PPRQLD DOLEUDWRU IURP :LH HU ODE )L DO UHDFWLR YRO PH P/

) (
3URYLGHG HDJHQWV UHDG WR VH 3 ( (
, W R F YHWWHV PDL WDL HG DW WKH VHOHFWHG WHPSHUDW UH
: ODEHOHG % EOD N D G 8 VDPSOH RU FDOLEUDWRU SODFH
The reagents are for "in vitro" diagnostic use. % 8
8VH WKH UHD H WV DFFRUGL WR WKH RUNL SURFHG UHV IRU
FOL LFDO ODERUDWRULHV DPSOH RU DOLEUDWRU /
OO UHD H WV D G VDPSOHV P VW EH GLVFDUGHG DFFRUGL WR LVWLOOHG ZDWHU /
ORFDO UH ODWLR V L IRUFH
HDJHQW P/ P/
/ ( 0L D G SUH L F EDWH IRU VHFR GV 5HDG 2 DEVRU
3URYLGHG HDJHQWV VWDEOH L UHIUL HUDWRU R
WLO WKH ED FH LPPHGLDWHO D G PHDV UH 2 DIWHU H DFWO
H SLUDWLR GDWH VKR L WKH ER PL WHV

/ ( ( ) ( (
The reagent solution should be clear. If turbid, the reagent / /
PD EH GHWHULRUDWHG PPR LD G/ ∆ 0
IDFWRU ∆ 2 2
G/
3/( )DFWRU
Plasma with EDTA ∆
6HH 3URFHG UH /LPLWDWLR V KHUH
D ROOHFWLRQ FROOHFW WKH VDPSOH DYRLGL VWDVLV D G KH ∆ 0 ∆ EVRUED FH RI WKH VDPSOH
 G/ DPPR LD FR FH WUDWLR L WKH DOLEUDWRU
∆ ∆ EVRUED FH RI WKH DOLEUDWRU

/ / (
PPRQLD RQWURO IURP :LH HU ODE

()( ( ( / (
Men: 25 - 94 ug/dL (14,7 - 55,3 umol/L)
:RPH G/ PRO /
(DFK ODERUDWRU VKR OG HVWDEOLVK LWV R UHIHUH FH YDO HV
The results should be assessed together with the patient’s
PHGLFDO EDFN UR G PHGLFDO H DPL DWLR D G RWKHU ODERUD
tory ndings.

( (
Ammonia (ug/dL) = 1,703 x ammonia (umol/L)

3 ( (/
6HH . R L WHUIHUL V EVWD FHV GHU 6 03/(
R RW VH VHU P DV D VDPSOH VL FH G UL FRD ODWLR LW
FD EH RUL L DWH DPPR LD SURG FL HUUR HR V UHV OWV
0D DOO GLO WH VDPSOHV WKDW DUH DERYH WKH PHDV UL UD H
with ammonia-free freshly distilled or deionized water (e.g. 1
0 OWLSO WKH UHV OW E WKH GLO WLR IDFWRU H
YRLG UHSRUWL WKH UHV OWV H HUDWHG IURP D H D WRPDWHG
U OHVV D IUHVK VDPSOH LV DGGHG

3( ) (
D HSURG FLELOLW HYDO DWHG DFFRUGL WR (3 SURWRFRO
from CLSI. This study used two di erent samples of ammonia
1+ WKDW HUH WHVWHG SHUIRUPL GDLO U V E G SOLFDWH
G UL GD V
QWUD DVVD SUHFLVLRQ
/HYHO 6 9
71,9 ug/dL
G/
E HWHFWLRQ OLPLW WKH GHWHFWLR OLPLW LV G/ PRO /
RI DPPR LD
F /LQHDULW the reaction is linear up to 1700 ug/dL(1000 umol/L)
RI DPPR LD

3 ( ( ) / (
)RU SUR UDPPL L VWU FWLR V UHIHU WR WKH VHU PD DO RI
the analyzer in use.

:( ( / 3 (
- 1 x 20 ml (Cat. Nº 1009693)
- 1 x 20 ml (Cat. Nº 1009911)
- 1 x 20 ml (Cat. Nº 1008146)

()( ( (
- Tietz, N.W., Clinical Guide to Laboratory Tests. 3 UG HG
3KLODGHOSKLD 3 : % 6D GHUV R
- Young, D.S., E ects of preanalytical variables on clinical
laboratory test. AACC Press. Third ed., 2007.
- Mondzac A, Ehrlich GE, Seegmiller JE - An enzymatic
determination of ammonia in biological uids - J. Lab. Clin.
0HG