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    1. Biotechnology deals with using live organisms or enzymes from organisms to produce products. It encompasses both traditional views and modern molecular biotechnology. 2. Restriction endonucleases like EcoRI and HindII cut DNA at specific recognition sequences, producing "sticky ends" or overhangs that allow targeted joining of DNA fragments. 3. Polymerase chain reaction (PCR) allows targeted amplification of specific DNA fragments in vitro through repeated cycles of denaturation, primer annealing and extension, using a thermostable DNA polymerase. Multiple copies of the gene of interest are synthesized.

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    1. Biotechnology deals with using live organisms or enzymes from organisms to produce products. It encompasses both traditional views and modern molecular biotechnology. 2. Restriction endonucleases like EcoRI and HindII cut DNA at specific recognition sequences, producing "sticky ends" or overhangs that allow targeted joining of DNA fragments. 3. Polymerase chain reaction (PCR) allows targeted amplification of specific DNA fragments in vitro through repeated cycles of denaturation, primer annealing and extension, using a thermostable DNA polymerase. Multiple copies of the gene of interest are synthesized.

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    © All Rights Reserved
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    6 views5 pages

    Biotechnology

    Uploaded by

    Arijit Mondal
    1. Biotechnology deals with using live organisms or enzymes from organisms to produce products. It encompasses both traditional views and modern molecular biotechnology. 2. Restriction endonucleases like EcoRI and HindII cut DNA at specific recognition sequences, producing "sticky ends" or overhangs that allow targeted joining of DNA fragments. 3. Polymerase chain reaction (PCR) allows targeted amplification of specific DNA fragments in vitro through repeated cycles of denaturation, primer annealing and extension, using a thermostable DNA polymerase. Multiple copies of the gene of interest are synthesized.

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    of 5

    Neutron Neet 2024 Zoology Sankalp

    Biotechnology: Principle and processes


    PNS – 02
    Statement Based

    1. Statement I: Biotechnology deals with (b) Statement I is correct but statement


    the techniques of using live organisms or II is incorrect
    enzyme from organisms to produce (c) Statement II is correct but statement
    products I is incorrect
    Statement II: According to EFB (d) Both the statements are incorrect
    biotechnology encompasses both
    tradition view and modern molecular 5. Statement I: Eco RI recognizes a 5 base
    biotechnology [NCERT Page No-163] pair sequence
    (a) Both the statements are correct Statement II: Hind II is a restriction
    (b) Statement I is correct but statement exonuclease [NCERT Page No-166]
    II is incorrect (a) Both the statements are correct
    (c) Statement II is correct but statement (b) Statement I is correct but statement
    I is incorrect II is incorrect
    (d) Both the statements are incorrect (c) Statement II is correct but statement
    I is incorrect
    2. Statement I: Asexual reproduction (d) Both the statements are incorrect
    provides opportunities for variations
    Statement II: Asexual reproduction 6. Statement I: The cutting of DNA at
    preserves the genetic information while specific location became possible with
    sexual reproduction permits variation the discovery of molecular scissors
    [NCERT Page No-164] Statement II: The plasmid DNA act as
    (a) Both the statements are correct an vector to transfer the piece of DNA
    (b) Statement I is correct but statement [NCERT Page No- 165]
    II is incorrect (a) Both the statements are correct
    (c) Statement II is correct but statement (b) Statement I is correct but statement
    I is incorrect II is incorrect
    (d) Both the statements are incorrect (c) Statement II is correct but statement
    I is incorrect
    3. Statement I: Genetic engineering (d) Both the statements are incorrect
    includes creation of RDNA, use of gene
    cloning and gene transfer 7. Statement I: The first discovered
    Statement II: Maintenance of sterile restriction endonuclease was Hind II
    conditions ensures to enable growth of Statement II: Hind II always cut DNA at
    desired and undesired product particular point by recognizing specific
    [NCERT Page No-163] sequence of 8 base pairs
    (a) Both the statements are correct [NCERT Page No-165]
    (b) Statement I is correct but statement (a) Both the statements are correct
    II is incorrect (b) Statement I is correct but statement
    (c) Statement II is correct but statement II is incorrect
    I is incorrect (c) Statement II is correct but statement
    (d) Both the statements are incorrect I is incorrect
    (d) Both the statements are incorrect
    4. Statement I: Ori is a DNA sequence
    responsible for initiating replication 8. Statement I: Roman numbers in the
    Statement II: Traditional hybridization name of restriction enzyme indicates the
    used in plants and animal breeding leads order of discovery
    to occurrence of undesirable gene Statement II: Each restriction
    [NCERT Page No-169] endonuclease recognizes a specific
    (a) Both the statements are correct palindromic sequence in the DNA
    #SankalpSelectionKa
    Neutron Neet 2024 Zoology Sankalp
    Biotechnology: Principle and processes
    PNS – 02
    [NCERT Page No-166] Statement II: In PCR desirable DNA is
    (a) Both the statements are correct modified and amplified
    (b) Statement I is correct but statement [NCERT Page No-173]
    II is incorrect (a) Both the statements are correct
    (c) Statement II is correct but statement (b) Statement I is correct but statement
    I is incorrect II is incorrect
    (d) Both the statements are incorrect (c) Statement II is correct but statement
    I is incorrect
    9. Statement I: EcoR1 a restriction enzyme (d) Both the statements are incorrect
    creates sticky ends while cutting the
    DNA
    13. Statement I: DNA is a negatively
    Statement II: if DNA molecule is cut by
    charged molecule hence it moves
    same restriction enzyme it creates same
    towards the cathode
    kind of sticky ends in DNA fragment
    Statement II: Ligase joins the fragment
    [NCERT Page No-167]
    of DNA [NCERT Page No-172]
    (a) Both the statements are correct
    (a) Both the statements are correct
    (b) Statement I is correct but statement
    (b) Statement I is correct but statement
    II is incorrect
    II is incorrect
    (c) Statement II is correct but statement
    (c) Statement II is correct but statement
    I is incorrect
    I is incorrect
    (d) Both the statements are incorrect
    (d) Both the statements are incorrect
    10. Statement I: The DNA fragments can be
    14. Statement I: Each cycle of PCR has 3
    separated by a technique known as
    steps denaturation, primer annealing,
    biolistic
    extension sequentially
    Statement II: Ori is also responsible for
    Statement II: New segment of DNA is
    controlling copy number of the linked
    formed in 5’to 3’ direction always
    DNA [NCERT Page No-168]
    [NCERT Page No-172]
    (a) Both the statements are correct (a) Both the statements are correct
    (b) Statement I is correct but statement (b) Statement I is correct but statement
    II is incorrect II is incorrect
    (c) Statement II is correct but statement (c) Statement II is correct but statement
    I is incorrect I is incorrect
    (d) Both the statements are incorrect (d) Both the statements are incorrect

    11. Statement I: PCR stands for polymerase 15. Statement I: In gene gun method cells
    chain reaction are bombarded with high velocity
    Statement II: In PCR multiple copies of microparticles of gold or tungsten coated
    gene of interest is synthesized in vitro with DNA
    two set of primers Statement II: Gene gun is also known as
    [NCERT Page No-172] biolistic method
    (a) Both the statements are correct [NCERT Page No-171]
    (b) Statement I is correct but statement (a) Both the statements are correct
    II is incorrect (b) Statement I is correct but statement
    (c) Statement II is correct but statement II is incorrect
    I is incorrect (c) Statement II is correct but statement
    (d) Both the statements are incorrect I is incorrect
    (d) Both the statements are incorrect
    12. Statement I: In PCR a thermostable
    DNA polymerase which remains active at 16. Statement I: In a method known as
    lower temperature is employed microinjection recombinant DNA is

    #SankalpSelectionKa
    Neutron Neet 2024 Zoology Sankalp
    Biotechnology: Principle and processes
    PNS – 02
    directly injected into nucleus of animal I is incorrect
    cell (d) Both the statements are incorrect
    Statement II: Isolation of genetic
    material from bacterial cell can be 20. Statement I: In a cloning vector it needs
    achieved by treating them with lysozyme to have preferably one or more than one
    [NCERT Page No- 171] restriction site for commonly used
    (a) Both the statements are correct restriction enzymes
    (b) Statement I is correct but statement Statement II: DNA is a hydrophobic
    II is incorrect molecule it can’t pass through cell
    (c) Statement II is correct but statement membranes directly
    I is incorrect [NCERT Page No-169&170]
    (d) Both the statements are incorrect (a) Both the statements are correct
    (b) Statement I is correct but statement
    17. Statement I: The Ti plasmid of II is incorrect
    agrobacterium has now been modified (c) Statement II is correct but statement
    into a cloning vector I is incorrect
    StatementII: Agrobacterium (d) Both the statements are incorrect
    tumefaciens a pathogen of monocots
    plants causes tumor in these plants 21. Statement I: A stirred tank reactor is
    [NCERT Page No-170] usually cylindrical or with a curved base
    (a) Both the statements are correct Statement II: The most commonly used
    (b) Statement I is correct but statement bioreactors are of stirring type
    II is incorrect [NCERT Page No-174]
    (c) Statement II is correct but statement (a) Both the statements are correct
    I is incorrect (b) Statement I is correct but statement
    (d) Both the statements are incorrect II is incorrect
    (c) Statement II is correct but statement
    18. Statement I: If any protein encoding I is incorrect
    gene is expressed in heterologous host it (d) Both the statements are incorrect
    is called recombinant protein
    Statement II: The cells can be multiplied 22. Statement I: Transformation is a
    in a continuous culture system to obtain procedure through which a piece of DNA
    the desired product is introduced in host bacterium
    [NCERT Page No-173] Statement II: pBR322 has restriction
    (a) Both the statements are correct sites for both Hind1 and HindII
    (b) Statement I is correct but statement [NCERT Page No-169]
    II is incorrect (a) Both the statements are correct
    (c) Statement II is correct but statement (b) Statement I is correct but statement
    I is incorrect II is incorrect
    (d) Both the statements are incorrect (c) Statement II is correct but statement
    I is incorrect
    19. Statement I: The most commonly used (d) Both the statements are incorrect
    matrix in gel electrophoresis is agarose
    Statement II: DNA separated by gel
    electrophoresis can be visualized only 23. Statement I: Selection of transformants
    after staining from non transformants can be done by
    [NCERT Page No-168] antibiotic resistance gene
    (a) Both the statements are correct Statement II: In insertional inactivation,
    (b) Statement I is correct but statement protein gets inactivated
    II is incorrect [NCERT Page No-170]
    (c) Statement II is correct but statement (a) Both the statements are correct

    #SankalpSelectionKa
    Neutron Neet 2024 Zoology Sankalp
    Biotechnology: Principle and processes
    PNS – 02
    (b) Statement I is correct but statement
    II is incorrect
    (c) Statement II is correct but statement
    I is incorrect
    (d) Both the statements are incorrect

    24. Statement I: The first step in RDT is


    isolation of genetic material
    Statement II: Genetic material of plants
    cells can be isolated with the help of
    cellulase [NCERT Page No-171]
    (a) Both the statements are correct
    (b) Statement I is correct but statement
    II is incorrect
    (c) Statement II is correct but statement
    I is incorrect
    (d) Both the statements are incorrect

    25. Statement I: DNA ligase is an


    endonuclease
    Statement II: It cuts DNA at
    palindromic sites
    [NCERT Page No-165]
    (a) Both the statements are correct
    (b) Statement I is correct but statement
    II is incorrect
    (c) Statement II is correct but statement
    I is incorrect
    (d) Both the statements are incorrect

    #SankalpSelectionKa
    Neutron Neet 2024 Zoology Sankalp
    Biotechnology: Principle and processes
    PNS – 02
    Answer Key

    S1. Ans. (a)


    S14. Ans. (a)
    S2. Ans. (c)
    S15. Ans. (a)
    S3. Ans. (b)
    S16. Ans. (a)
    S4. Ans. (a)
    S17. Ans. (b)
    S5. Ans. (d)
    S18. Ans. (a)
    S6. Ans. (a)
    S19. Ans. (a)
    S7. Ans. (b)
    S20. Ans. (b)
    S8. Ans. (c)
    S21. Ans. (a)
    S9. Ans. (a)
    S22. Ans. (b)
    S10. Ans. (c)
    S23. Ans. (b)
    S11. Ans. (a)
    S24. Ans. (a)
    S12. Ans. (d)
    S25. Ans. (d)
    S13. Ans. (c)

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