Molecular Cloning

This is a free sample of content from Molecular Cloning: A Laboratory Manual, 4th edition.
Click here for more information or to buy the book.
UNKTLD 0
LnkdbtkYq Bknmhmf
? K?ANQ?SNQX L?MT?K
ENTQSG DCHSHNM
© 2012 by Cold Spring Harbor Laboratory Press

NSGDQ SHSKDR EQNL BRGK OQDRR
K:ANQ:SNQX L:MT:KR
:mshanchdr2 : IVanpVsnpw LVmtVk
HlVfhmf2 : IVanpVsnpw LVmtVk
Ihud Bdkk HlVfhmf2 : IVanpVsnpw LVmtVk’ .mc Dchshnm
LVmhotkVshmf sgd Lntrd Clapwn2 : IVanpVsnpw LVmtVk’ 2qc Dchshnm
QM:2 : IVanpVsnpw LVmtVk
G:MCANNIR
IVa LVsg2 : EVmcanni ne LdVrtpdldmsr BVkbtkVshnmr Vmc Nsgdp PtVmshsVshud Rihkkr
enp Trd Vs sgd Admbg
IVa Qde Unktld .2 : EVmcanni ne Qdbhodr QdVfdmsr Vmc Nsgdp Qdedpdmbd Snnkr enp
Trd Vs sgd Admbg
IVa Qde Unktld 12 : EVmcanni ne Qdbhodr QdVfdmsr Vmc Nsgdp Qdedpdmbd Snnkr enp
Trd Vs sgd Admbg
RsVshrshbr Vs sgd Admbg2 : Rsdo,aw,Rsdo EVmcanni enp Ahnknfhrsr
VDARHSDR
LnkdbtkVp Bknmhmf : IVanpVsnpw LVmtVk 3sg Dchshnm vvv-lnkdbtkVpbknmhmf-npf
Bnkc Rophmf EVpanp Opnsnbnkr vvv-brgopnsnbnkr-npf

UNKTLD 0
LnkdbtkZq Bknmhmf
? K?ANQ?SNQX L?MT?K
ENTQSG DCHSHNM
LhbgZdk Q- Fqddm
GnvUpc Gtfgdr IdchbUi Hmrshstsd
OpnfpUlr hm Fdmd Etmbshnm Umc Dwopdrrhnm Umc hm InidbtiUp Idchbhmd
Tmhudprhsx ne IUrrUbgtrdssr IdchbUi Pbgnni
Inrdog RZlaqnnj
Odsdp IUbAUiitl AUmbdp Admspd Umc sgd
Odsdp IUbAUiitl CdoUpsldms ne Mmbninfx
Sgd Tmhudprhsx ne Idiantpmd 4trspUihU
BNIC ROPHME G4PANP I4ANP4SNPT OPDRR

Bnkc Roqhmf GYqanq’ Mdv Tnqj vvv-brgkoqdrr-nqf

LnkdbtkYq Bknmhmf
? K?ANQ?SNQX L?MT?K
ENTQSG DCHSHNM
1/01 ax Bnkc Roqhmf GZqanq KZanqZsnqx Oqdrr) Bnkc Roqhmf GZqanq) Mdv Wnqj
;kk qhfgsr qdrdqudc
Oqhmsdc hm sgd Tmhsdc RsZsdr ne ;ldqhbZ
Mtakhrgdq Ingm Hmfkhr

:bpthrhshnm Dchsnqr Ingm Hmfkhr) ;mm Anxkd) ;kdwZmcdq FZmm
Chqdbsnq ne Cdudknoldms& ESqjdshmf& PSkdr IZm ;qfdmshmd
CdudknoldmsSk Dchsnqr Itcx Btcchgx) JZZqdm IZmrrdm) LhbgZdk Yhdqkdq
Mqnidbs ESmSfdq LZqxkhy Chbjdqrnm
Mdqlhrrhnmr AnnqchmSsnq BZqnk Aqnvm
Mqnctbshnm Dchsnq JZsgkddm Ataadn
Mqnctbshnm ESmSfdq Cdmhrd Vdhrr
PSkdr :bbntms ESmSfdq DkhyZadsg Onvdqr
Anudq Sqs chqdbshnm Smc cdrhfm9 Odsd Ideer) 1/01
KhaqZqx ne Bnmfqdrr BZsZknfhmf,hm,OtakhbZshnm CZsZ
Fqddm) LhbgZdk Q- LhbgZdk QhbgZqc() 0843,

LnkdbtkZq bknmhmf 9 Z kZanqZsnqx lZmtZk . LhbgZdk Q- Fqddm)
Inrdog RZlaqnnj- “ 3sg dc-
o- bl-
Qdu- dc- ne9 LnkdbtkZq bknmhmf 9 Z kZanqZsnqx lZmtZk . Inrdog
RZlaqnnj) CZuhc V- Qtrrdkk- 1//0-
Hmbktcdr ahakhnfqZoghbZk qdedqdmbdr Zmc hmcdw-
HRAM 867,0,825002,30,4 bknsg( “ HRAM 867,0,825002,31,1
oaj-(
0- LnkdbtkZq bknmhmf“KZanqZsnqx lZmtZkr- H- RZlaqnnj) Inrdog-
HH- RZlaqnnj) Inrdog- LnkdbtkZq bknmhmf- HHH- Shskd-
PG331-1-R15 1/01
461-7“cb12
1/01//1502
0, 8 7 6 5 4 3 2 1
Rstcdmsr Zmc qdrdZqbgdqr trhmf sgd oqnbdctqdr hm sghr lZmtZk cn rn Zs sgdhq nvm qhrj- Bnkc Roqhmf GZqanq KZanqZsnqx lZjdr mn qdoqd,
rdmsZshnmr nq vZqqZmshdr vhsg qdrodbs sn sgd lZsdqhZk rds enqsg hm sghr lZmtZk Zmc gZr mn khZahkhsx hm bnmmdbshnm vhsg sgd trd ne sgdrd
lZsdqhZkr- ;kk qdfhrsdqdc sqZcdlZqjr) sqZcd mZldr) Zmc aqZmc mZldr ldmshnmdc hm sghr annj Zqd sgd oqnodqsx ne sgd qdrodbshud nvmdqr-
QdZcdqr rgntkc okdZrd bnmrtks hmchuhctZk lZmteZbstqdqr Zmc nsgdq qdrntqbdr enq btqqdms Zmc rodbhehb oqnctbs hmenqlZshnm-
Vhsg sgd dwbdoshnm ne sgnrd rtookhdqr khrsdc hm sgd sdws vhsg sgdhq Zccqdrrdr) Zkk rtookhdqr ldmshnmdc hm sghr lZmtZk bZm ad entmc nm sgd
AhnRtookxMds Vda rhsd Zs vvv-ahnrtookxmds-bnl-
;kk Vnqkc Vhcd Vda Zccqdrrdr Zqd ZbbtqZsd sn sgd adrs ne ntq jmnvkdcfd Zs sgd shld ne oqhmshmf-
Oqnbdctqdr enq sgd gtlZmd sqdZsldms ne ZmhlZkr ltrs ad nardqudc Zs Zkk shldr- Bgdbj vhsg sgd knbZk ZmhlZk eZbhkhsx enq fthcdkhmdr-
BdqsZhm dwodqhldmsZk oqnbdctqdr hm sghr lZmtZk lZx ad sgd rtaidbs ne mZshnmZk nq knbZk kdfhrkZshnm nq Zfdmbx qdrsqhbshnmr- Trdqr ne sghr
lZmtZk Zqd qdronmrhakd enq nasZhmhmf sgd qdkduZms odqlhrrhnmr) bdqshehbZsdr) nq khbdmrdr hm sgdrd bZrdr- Mdhsgdq sgd Ztsgnqr ne sghr lZmtZk
mnq Bnkc Roqhmf GZqanq KZanqZsnqx Zrrtld Zmx qdronmrhahkhsx enq eZhktqd ne Z trdq sn cn rn-
Sgd lZsdqhZkr Zmc ldsgncr hm sghr lZmtZk lZx hmeqhmfd sgd oZsdms Zmc oqnoqhdsZqx qhfgsr ne nsgdq hmchuhctZkr) bnloZmhdr) nq nqfZmhyZ,
shnmr- Trdqr ne sghr lZmtZk Zqd qdronmrhakd enq nasZhmhmf Zmx khbdmrdr mdbdrrZqx sn trd rtbg lZsdqhZkr Zmc sn oqZbshbd rtbg ldsgncr- BNKC
ROQHMF G;QANQ K;ANQ;SNQW L;JDR MN V;QQ;MSW NQ QDOQDRDMS;SHNM SG;S TRD NE SGD HMENQL;SHNM HM SGHR
L;MT;K VHKK MNS HMEQHMFD ;MW O;SDMS NQ NSGDQ OQNOQHDS;QW QHFGS-
;tsgnqhyZshnm sn ognsnbnox hsdlr enq hmsdqmZk nq odqrnmZk trd) nq sgd hmsdqmZk nq odqrnmZk trd ne rodbhehb bkhdmsr) hr fqZmsdc ax Bnkc Roqhmf
GZqanq KZanqZsnqx Oqdrr) oqnuhcdc sgZs sgd ZooqnoqhZsd edd hr oZhc chqdbskx sn sgd Bnoxqhfgs BkdZqZmbd Bdmsdq BBB(- Vqhsd nq bZkk BBB
Zs 111 Qnrdvnnc Cqhud) CZmudqr) L; /0812 867,64/,73//( enq hmenqlZshnm Zants eddr Zmc qdftkZshnmr- Oqhnq sn ognsnbnoxhmf hsdlr enq
dctbZshnmZk bkZrrqnnl trd) bnmsZbs BBB Zs sgd Zanud Zccqdrr- ;cchshnmZk hmenqlZshnm nm BBB bZm ad nasZhmdc Zs BBB Nmkhmd Zs vvv-
bnoxqhfgs-bnl-
Enq Z bnlokdsd bZsZknf ne Zkk Bnkc Roqhmf GZqanq KZanqZsnqx Oqdrr OtakhbZshnmr) uhrhs ntq vdarhsd Zs vvv-brgkoqdrr-nqf-

Bnmsdmsr
Khrs ne SZakdr wwu

OqdeZbd wwwh
?bjmnvkdcfldmsr wwwhhh
UNKTLD 0
O:QS 09 DRRDMSH:KR
BG:OSDQ 0
HrnkYshnm Ymc PtYmshehbYshnm ne CM: 0
HMSQNCTBSHNM
CM? HrnkZshnm 1
BnlldqbhZk Jhsr enq OtqhehbZshnm ne CM? 2
PtZmshexhmf CM? 4
OQNSNBNKR
0 OqdoZqZshnm ne OkZrlhc CM? ax ?kjZkhmd Kxrhr vhsg RCR9 Lhmhoqdor 00
1 OqdoZqZshnm ne OkZrlhc CM? ax ?kjZkhmd Kxrhr vhsg RCR9 LZwhoqdor 04
2 HrnkZshmf CM? eqnl FqZl,MdfZshud AZbsdqhZ %d-f- D- bnkh( 08
3 OqdbhohsZshnm ne CM? vhsg DsgZmnk 10
4 OqdbhohsZshnm ne CM? vhsg HrnoqnoZmnk 15
5 BnmbdmsqZshmf Zmc CdrZkshmf Mtbkdhb ?bhcr vhsg LhbqnbnmbdmsqZsnqr 17
6 BnmbdmsqZshmf Mtbkdhb ?bhcr ax DwsqZbshnm vhsg AtsZmnk 2/
7 OqdoZqZshnm ne Rhmfkd,RsqZmcdc AZbsdqhnogZfd L02 CM? ax OqdbhohsZshnm vhsg
Onkxdsgxkdmd Fkxbnk 20
8 OkZshmf AZbsdqhnogZfd L02 23
0. Fqnvhmf AZbsdqhnogZfd L02 hm Khpthc Btkstqd 27
00 OqdoZqZshnm ne Cntakd,RsqZmcdc %QdokhbZshud Enql( AZbsdqhnogZfd L02 CM? 30
01 HrnkZshnm ne Ghfg,LnkdbtkZq,Vdhfgs CM? Trhmf NqfZmhb Rnkudmsr sn Otqhex CM? 33
02 HrnkZshnm ne Ghfg,LnkdbtkZq,Vdhfgs CM? eqnl LZllZkhZm Bdkkr
Trhmf OqnsdhmZrd J Zmc Ogdmnk 36

uh Bnmsdmsr
03 ? Rhmfkd,Rsdo Ldsgnc enq sgd RhltksZmdntr OqdoZqZshnm ne CM?) QM?) Zmc Oqnsdhm
eqnl Bdkkr Zmc Shrrtdr 43
04 OqdoZqZshnm ne Fdmnlhb CM? eqnl Lntrd SZhkr Zmc Nsgdq RlZkk RZlokdr 47
?ksdqmZshud Oqnsnbnk9 HrnkZshnm ne CM? eqnl Lntrd SZhkr vhsgnts
DwsqZbshnm ax NqfZmhb Rnkudmsr 50
?ksdqmZshud Oqnsnbnk9 Nmd,Stad HrnkZshnm ne CM? eqnl Lntrd SZhkr 51
05 QZohc HrnkZshnm ne XdZrs CM? 53
06 Trhmf Dsghchtl Aqnlhcd sn DrshlZsd sgd ?lntms ne CM? hm AZmcr Zesdq
Dkdbsqnognqdrhr sgqntfg Lhmhfdkr 55
07 DrshlZshmf sgd BnmbdmsqZshnm ne CM? ax Ektnqnldsqx Trhmf Gndbgrs 22147 57
08 PtZmshexhmf CM? hm Rnktshnm vhsg OhbnFqddm 60
HMENQL:SHNM O:MDKR
CM? DwsqZbshnm eqnl EnqlZkcdgxcd,Ehwdc Shrrtd Dladccdc hm OZqZeehm Aknbjr 61
Onkxdsgxkdmd Fkxbnk 62
,BnlokdldmsZshnm 63
W,FZk 65
Lhmhlhyhmf CZlZfd sn KZqfd CM? Lnkdbtkdr 66
Rodbsqnognsnldsqx 67
BG:OSDQ 1
:mYkxrhr ne CM: 70
HMSQNCTBSHNM
?fZqnrd Fdk Dkdbsqnognqdrhr 71
?mZkxrhr ne CM? EqZfldmsr 75
Qdbnudqhmf CM? eqnl Fdkr 76
Rntsgdqm Aknsshmf 77
OQNSNBNKR
0 ?fZqnrd Fdk Dkdbsqnognqdrhr 83
1 Cdsdbshnm ne CM? hm ?fZqnrd Fdkr ax RsZhmhmf 88
2 OnkxZbqxkZlhcd Fdk Dkdbsqnognqdrhr 0/3
3 Cdsdbshnm ne CM? hm OnkxZbqxkZlhcd Fdkr ax RsZhmhmf 00/
4 Cdsdbshnm ne CM? hm OnkxZbqxkZlhcd Fdkr ax ?tsnqZchnfqZogx 001
5 ?kjZkhmd ?fZqnrd Fdk Dkdbsqnognqdrhr 003
?cchshnmZk Oqnsnbnk9 ?tsnqZchnfqZogx ne ?kjZkhmd ?fZqnrd Fdkr 006
6 HlZfhmf9 ?tsnqZchnfqZogx Zmc OgnrognqhlZfhmf 008
7 Qdbnudqx ne CM? eqnl ?fZqnrd Fdkr Trhmf FkZrr AdZcr 014
8 Qdbnudqx ne CM? eqnl Knv,Ldkshmf,SdlodqZstqd
?fZqnrd Fdkr9 NqfZmhb DwsqZbshnm 016
0. HrnkZshnm ne CM? EqZfldmsr eqnl OnkxZbqxkZlhcd Fdkr ax sgd Bqtrg
Zmc RnZj Ldsgnc 02/
00 Rntsgdqm Aknsshmf 022
01 Rntsgdqm Aknsshmf9 RhltksZmdntr SqZmredq ne CM? eqnl Zm ?fZqnrd
Fdk sn Svn LdlaqZmdr 030

Bnmsdmsr uhh
02 Rntsgdqm GxaqhchyZshnm ne QZchnkZadkdc Oqnadr sn Mtbkdhb ?bhcr

Hllnahkhydc nm LdlaqZmdr 033
?cchshnmZk Oqnsnbnk9 Rsqhoohmf Oqnadr eqnl LdlaqZmdr 04/
HMENQL:SHNM O:MDKR
EnqlZlhcd Zmc Hsr Trdr hm LnkdbtkZq Bknmhmf 042
QZohc GxaqhchyZshnm Ateedqr 044
BG:OSDQ 2
Bknmhmf Ymc SqYmrenqlYshnm vhsg OkYrlhc Udbsnqr 046
HMSQNCTBSHNM
OkZrlhc Udbsnqr 047
SqZmrenqlZshnm 048
OQNSNBNKR
0 Sgd GZmZgZm Ldsgnc enq OqdoZqZshnm Zmc SqZmrenqlZshnm ne Bnlodsdms D- bnkh9
Ghfg,Deehbhdmbx SqZmrenqlZshnm 051
1 Sgd Hmntd Ldsgnc enq OqdoZqZshnm Zmc SqZmrenqlZshnm ne Bnlodsdms D- bnkh9
zTksqZbnlodsdms– Bdkkr 057
2 DZrx SqZmrenqlZshnm ne D- bnkh1 MZmnoZqshbkd,LdchZsdc SqZmrenqlZshnm 062
?ksdqmZshud Oqnsnbnk9 Nmd,Rsdo OqdoZqZshnm ne Bnlodsdms D- bnkh9 SqZmrenqlZshnm
Zmc RsnqZfd ne AZbsdqhZk Bdkkr hm sgd RZld Rnktshnm 064
3 SqZmrenqlZshnm ne D- bnkh ax DkdbsqnonqZshnm 066
4 Bknmhmf hm OkZrlhc Udbsnqr9 ChqdbshnmZk Bknmhmf 072
5 Bknmhmf hm OkZrlhc Udbsnqr9 Aktms,Dmc Bknmhmf 075
6 CdognrognqxkZshnm ne OkZrlhc CM? 078
7 ?ssZbghmf OgnrognqxkZsdc ?cZosnqr Khmjdqr sn Aktms,Dmcdc CM?r 081
8 Bknmhmf OBQ Oqnctbsr9 ?cchshnm ne Qdrsqhbshnm Rhsdr sn sgd Sdqlhmh ne ?lokhehdc CM? 083
0. Bknmhmf OBQ Oqnctbsr9 Aktms,Dmc Bknmhmf 086
00 Bknmhmf OBQ Oqnctbsr9 LZjhmf S Udbsnqr 1//
01 Bknmhmf OBQ Oqnctbsr9 S? Bknmhmf 1/2
02 Bknmhmf OBQ Oqnctbsr9 SNON S? Bknmhmf 1/5
03 Rbqddmhmf AZbsdqhZk Bnknmhdr Trhmf W,FZk Zmc HOSF9 ,BnlokdldmsZshnm 10/
HMENQL:SHNM O:MDKR
BZqhmf enq D- bnkh 102
Sgd Ghrsnqx ne SqZmrenqlZshnm ne AZbsdqhZ ax CM? 106
? Fthcd sn Bknmhmf sgd Oqnctbsr ne OnkxldqZrd BgZhm QdZbshnmr 107
AhnAqhbjr Zmc sgd Nqcdqdc ?rrdlakx ne CM? EqZfldmsr 114
SNON Snnkr9 BqdZshmf KhmdZq Dwoqdrrhnm Bnmrsqtbsr vhsg EtmbshnmZk Dkdldmsr 116
?mshahnshbr 118
?cZosnqr 121
Khmjdqr 123
KhfZshnm Zmc KhfZrdr 124

uhhh Bnmsdmsr
Bnmcdmrhmf Zmc Bqnvchmf QdZfdmsr 13/

Sgd Chrbnudqx ne Qdrsqhbshnm Dmyxldr 130
Qdrsqhbshnm Dmyxldr 131
BgknqZlogdmhbnk 134
Sgd bbcA Fdmd 136
AZbsdqhnogZfd i 137
AZbsdqhnogZfd L02 138
OkZrlhcr 140
Bnrlhcr 147
BG:OSDQ 3
FYsdvYx QdbnlahmYshnmYk Bknmhmf 150
Ingm R- Qddbd,Gnxdr Zmc :kadqsgZ I-L- UZkgnts
HMSQNCTBSHNM
AZrhb Oqhmbhokdr Zmc ?ookhbZshnmr ne FZsdvZx Bknmhmf 151
ChrZcuZmsZfdr ne FZsdvZx Bknmhmf Zmc ?ksdqmZshud Bknmhmf Rxrsdlr 153
OQNSNBNKR
0 OqnoZfZshmf FZsdvZx Udbsnqr 156
1 FdmdqZshmf Zm NQE Dmsqx Bknmd Zmc CdrshmZshnm Bknmd 16/
2 Trhmf Ltkshrhsd KQ Bknmhmf sn FdmdqZsd Z CdrshmZshnm Bknmd 166
HMENQL:SHNM O:MDK
FdmdqZshmf FZsdvZx,BnloZshakd Udbsnqr 17/
BG:OSDQ 4
Vnqihmf vhsg AYbsdqhYk :qshehbhYk Bgqnlnrnldr Ymc Nsgdq Ghfg,BYoYbhsx Udbsnqr 170
MZsgZmhdk Gdhmsy Zmc RghZnbghmf Fnmf
HMSQNCTBSHNM
Cdudknoldms ne Ghfg,BZoZbhsx Udbsnqr9 ?cuZmsZfdr Zmc ChrZcuZmsZfdr 171
Vnqjhmf vhsg AZbsdqhZk ?qshehbhZk Bgqnlnrnldr 175
OQNSNBNKR
0 RlZkk,RbZkd HrnkZshnm ne A?B CM? Zmc UdqhehbZshnm ax OBQ 183
1 KZqfd,RbZkd OqdoZqZshnm Zmc KhmdZqhyZshnm ne A?B CM? 186
2 DwZlhmZshnm ne A?B CM? PtZkhsx Zmc PtZmshsx ax Otkrdc,Ehdkc Fdk
Dkdbsqnognqdrhr 2/0
3 Svn,Rsdo A?B Dmfhmddqhmf9 OqdoZqZshnm ne Rgtsskd Udbsnq CM? 2/2
4 OqdoZqZshnm ne sgd ? Gnlnknfx ?ql %?,Anw( Zmc A Gnlnknfx ?ql %A,Anw( 2/5
5 Bknmhmf ne sgd ? Zmc A Gnlnknfx ?qlr hmsn sgd Rgtsskd Udbsnq 2/8
6 OqdoZqZshnm Zmc UdqhehbZshnm ne sgd QdbnlahmZms Rgtsskd Udbsnq 201

Bnmsdmsr hw
7 DkdbsqnonqZshnm ne Bnlodsdms A?B Gnrs Bdkkr vhsg sgd QdbnlahmZms

Rgtsskd Udbsnq 204
8 UdqhehbZshnm ne BnhmsdfqZsdr Zmc Rdkdbshnm ne Qdrnkudc
A?B Bknmdr 206
0. Nmd,Rsdo A?B LnchehbZshnm9 OqdoZqZshnm ne OkZrlhcr 210
00 OqdoZqZshnm ne sgd ? Gnlnknfx ?ql %?,Anw( 213
01 Bknmhmf ne sgd ? Gnlnknfx ?ql hmsn Qdonqsdq,Rgtsskd Udbsnq 215
02 SqZmrenqlZshnm ne sgd A?B Gnrs vhsg sgd Qdb? Udbsnq 218
03 SqZmredq ne sgd Qdonqsdq Udbsnq hmsn A?B Qdb? Bdkkr Zmc Rdkdbshnm
ne BnhmsdfqZsdr 220
04 Fqnvsg ne R- bdqduhrhZd Zmc OqdoZqZshnm ne CM? 223
05 RlZkk,RbZkd OqdoZqZshnmr ne XdZrs CM? 225
HMENQL:SHNM O:MDKR
BQD “ knvO 227
Trhmf DFEO Zr Z Qdonqsdq 231
Oqhldq Cdrhfm enq Gnlnknfx ?qlr) BnhmsdfqZshnm) Zmc Qdrnktshnm 232
XdZrs LdchZ 233
BG:OSDQ 5
DwsqYbshnm) OtqhehbYshnm) Ymc :mYkxrhr ne QM: eqnl DtiYqxnshb Bdkkr 234
HMSQNCTBSHNM
OQNSNBNKR
Hmsqnctbshnm sn sgd HrnkZshnm ne SnsZk QM? Trhmf LnmnogZrhb Kxrhr QdZfdmsr 237
0 OtqhehbZshnm ne SnsZk QM? eqnl LZllZkhZm Bdkkr Zmc Shrrtdr 240
?ksdqmZshud Oqnsnbnk9 OqdoZqhmf QM? eqnl RlZkkdq RZlokdr 243
1 HrnkZshnm ne SnsZk QM? eqnl YdaqZehrg Dlaqxnr Zmc ?ctksr 244
2 SnsZk QM? HrnkZshnm eqnl CqnrnoghkZ ldkZmnfZrsdq 246
3 SnsZk QM? DwsqZbshnm eqnl BZdmnqgZachshr dkdfZmr 248
4 SnsZk QM? DwsqZbshnm eqnl RZbbgZqnlxbdr bdqduhrhZd Trhmf Gns
?bhc Ogdmnk 251
5 PtZmshexhmf Zmc Rsnqhmf QM? 254
6 OqdbhohsZshnm ne QM? vhsg DsgZmnk 261
7 Qdlnuhmf CM? BnmsZlhmZshnm eqnl QM? RZlokdr ax SqdZsldms vhsg
QMZrd,Eqdd CMZrd H 264
0
8 HrnkZshnm ne Onkx%?( Ldrrdmfdq QM? Trhmf LZfmdshb Nkhfn%cS( AdZcr 266
Hmsqnctbshnm sn GxaqhchyZshnm ne QM? ax Mnqsgdqm SqZmredq 270
0. RdoZqZshnm ne QM? Zbbnqchmf sn Rhyd9 Dkdbsqnognqdrhr ne QM? sgqntfg ?fZqnrd Fdkr
BnmsZhmhmf EnqlZkcdgxcd 277
00 RdoZqZshnm ne QM? Zbbnqchmf sn Rhyd9 Dkdbsqnognqdrhr ne QM? sgqntfg CdmZstqhmf TqdZ
OnkxZbqxkZlhcd Fdkr 282
01 SqZmredq Zmc EhwZshnm ne CdmZstqdc QM? hm ?fZqnrd Fdkr sn LdlaqZmdr 3/0
?ksdqmZshud Oqnsnbnk9 BZohkkZqx SqZmredq ax CnvmvZqc Eknv 3/5

w Bnmsdmsr
02 SqZmredq Zmc EhwZshnm ne CdmZstqdc QM? hm OnkxZbqxkZlhcd Fdkr sn LdlaqZmdr ax

Dkdbsqnognqdshb SqZmredq 3/7
03 Mnqsgdqm GxaqhchyZshnm 30/
04 Cns Zmc Rkns GxaqhchyZshnm ne Otqhehdc QM? 304
Hmsqnctbshnm sn LZoohmf QM? 31/
05 LZoohmf QM? vhsg MtbkdZrd R0 32/
06 QhanmtbkdZrd Oqnsdbshnm9 LZoohmf QM? vhsg QhanmtbkdZrd Zmc QZchnkZadkdc
QM? Oqnadr 326
07 ?mZkxrhr ne QM? ax Oqhldq Dwsdmrhnm 334
HMENQL:SHNM O:MDKR
Gnv sn Vhm sgd AZsskd vhsg QMZrd 34/
Hmghahsnqr ne QMZrdr 340
ChdsgxkoxqnbZqanmZsd 341
MtbkdZrd R0 343
BG:OSDQ 6
OnkxldqYrd BgYhm QdYbshnm 344
HMSQNCTBSHNM
Sgd AZrhb OnkxldqZrd BgZhm QdZbshnm 345
Cdrhfm ne Nkhfnmtbkdnshcd Oqhldqr enq AZrhb OBQ 351
Cdsdbshmf) ?mZkxyhmf) Zmc PtZmshexhmf lQM?r 353
BnmsZlhmZshnm hm OBQ 355
OQNSNBNKR
0 Sgd AZrhb OnkxldqZrd BgZhm QdZbshnm 36/
1 Gns RsZqs OBQ 366
2 Sntbgcnvm OBQ 370
3 OBQ ?lokhehbZshnm ne FB,Qhbg SdlokZsdr 373
4 Knmf Zmc ?bbtqZsd OBQ %K? OBQ( 38/
5 Hmudqrd OBQ 383
6 Mdrsdc OBQ 388
7 ?lokhehbZshnm ne bCM? FdmdqZsdc ax Qdudqrd SqZmrbqhoshnm ne lQM?9 Svn,Rsdo
QS,OBQ 4/2
8 QZohc ?lokhehbZshnm ne Rdptdmbdr eqnl sgd 4 Dmcr ne lQM?r9 4 ,Q?BD 404
0. QZohc ?lokhehbZshnm ne Rdptdmbdr eqnl sgd 2 Dmcr ne lQM?r9 2 ,Q?BD 412
00 Rbqddmhmf Bnknmhdr ax OBQ 420
HMENQL:SHNM O:MDKR
SZp CM? OnkxldqZrd 422
OBQ hm Sgdnqx 426
QhanmtbkdZrd G 427
Sgd cts Zmc tmf Fdmdr ne D- bnkh 428
SdqlhmZk SqZmredqZrd 43/

Bnmsdmsr wh
BG:OSDQ 7
AhnhmenqlYshbr 430
Ith,Gtmf Gtmf Zmc Yghohmf Udmf
HMSQNCTBSHNM
OQNSNBNKR
0 UhrtZkhyhmf Fdmnlhb ?mmnsZshnmr vhsg sgd TBRB Fdmnld Aqnvrdq 433
Hmsqnctbshnm sn Rdptdmbd ?khfmldms Zmc Gnlnknfx RdZqbg 443
1 Rdptdmbd ?khfmldms Zmc Gnlnknfx RdZqbg vhsg AK?RS Zmc BktrsZkV 446
2 Cdrhfmhmf OBQ Oqhldqr Trhmf Oqhldq2Oktr 453
Hmsqnctbshnm sn Dwoqdrrhnm Oqnehkhmf ax LhbqnZqqZx Zmc QM?,rdp 460
3 Dwoqdrrhnm Oqnehkhmf ax LhbqnZqqZx Zmc QM?,rdp 466
Hmsqnctbshnm sn LZoohmf Ahkkhnmr ne Rgnqs QdZcr sn Z Qdedqdmbd Fdmnld 477
4 LZoohmf Ahkkhnmr ne Rgnqs QdZcr sn Z Qdedqdmbd Fdmnld 480
Hmsqnctbshnm sn OdZj,Ehmchmf ?kfnqhsglr 487
5 Hcdmshexhmf Qdfhnmr Dmqhbgdc hm Z BgHO,rdp CZsZ Rds %OdZj Ehmchmf( 5/3
Hmsqnctbshnm sn Lnshe Ehmchmf 501
6 Chrbnudqhmf bhr,QdftkZsnqx Lnsher 506
HMENQL:SHNM O:MDKR
CZsZ EnqlZsr 514
?kfnqhsglr) OnqsZkr) Zmc Ldsgncr 517
UNKTLD 1
O:QS 19 :M:KXRHR :MC L:MHOTK:SHNM NE CM: :MC QM:
BG:OSDQ 8
PtYmshehbYshnm ne CM: Ymc QM: ax QdYk,Shld OnkxldqYrd BgYhm QdYbshnm 520
HMSQNCTBSHNM
QdZk,Shld OBQ Bgdlhrsqhdr 521
Hmrsqtldmsr enq QdZk,Shld OBQ 528
DwsqZbshmf CZsZ eqnl Z QdZk,Shld OBQ Dwodqhldms9 CZsZ ?mZkxrhr
Zmc MnqlZkhyZshnm Ldsgncr 530
Cdrhfmhmf Oqhldqr Zmc Oqnadr Zmc Noshlhyhmf Bnmchshnmr enq QdZk,Shld OBQ 532
Bnmrsqtbshmf Z RsZmcZqc Btqud 537
Odqenqlhmf QdZk,Shld OBQ 54/
Odqenqlhmf QdZk,Shld QS,OBQ 54/
LHPD Fthcdkhmdr 543
QdZk,Shld OBQ Oqnsnbnkr 543

whh Bnmsdmsr
OQNSNBNKR
0 Noshlhyhmf Oqhldq Zmc Oqnad BnmbdmsqZshnmr enq Trd hm QdZk,Shld OBQ 547
1 Bnmrsqtbshmf Z RsZmcZqc Btqud 552
2 PtZmshehbZshnm ne CM? ax QdZk,Shld OBQ 556
3 PtZmshehbZshnm ne QM? ax QdZk,Shld QS,OBQ 56/
4 ?mZkxrhr Zmc MnqlZkhyZshnm ne QdZk,Shld OBQ DwodqhldmsZk CZsZ 563
HMENQL:SHNM O:MDKR
Ltkshokdw OBQ 57/
RMO Fdmnsxohmf 570
BG:OSDQ 0.
Mtbkdhb :bhc OkYsenql Sdbgmnknfhdr 572
Nkhudq QZmcn
HMSQNCTBSHNM
LhbqnZqqZx ?ookhbZshnmr 574
Odqenqlhmf LhbqnZqqZx Dwodqhldmsr 577
OQNSNBNKR
0 Oqhmshmf LhbqnZqqZxr 583
1 Qntmc ? Qntmc A ?lokhehbZshnm ne CM? 587
2 S6 KhmdZq ?lokhehbZshnm ne CM? %SK?C( enq MtbkdnrnlZk
Zmc Nsgdq CM? 4// ao 6/1
3 ?lokhehbZshnm ne QM? 6/8
4 Chqdbs BxZmhmd,cTSO KZadkhmf ne QM? 604
5 Hmchqdbs ?lhmnZkkxk,cTSO KZadkhmf ne QM? 607
6 BxZmhmd,cBSO KZadkhmf ne CM? Trhmf Jkdmnv 610
7 Hmchqdbs KZadkhmf ne CM? 613
8 Aknbjhmf Onkxkxrhmdr nm GnldlZcd LhbqnZqqZxr 615
0. GxaqhchyZshnm sn GnldlZcd LhbqnZqqZxr 618
BG:OSDQ 00
CM: Rdptdmbhmf 624
DkZhmd LZqchr Zmc U- QhbgZqc LbBnlahd
HMSQNCTBSHNM
Ghrsnqx ne RZmfdq Chcdnwx CM? Rdptdmbhmf 625
Mdws,FdmdqZshnm Rdptdmbhmf 631
Nudquhdv ne Mdws,FdmdqZshnm Rdptdmbhmf Hmrsqtldmsr 641
RZmfdq Rdptdmbhmf udqrtr Mdws,FdmdqZshnm Rdptdmbhmf9 Vgdm sn Cn VgZs: 65/
Hmsqnctbshnm sn Oqnsnbnkr 650

Bnmsdmsr whhh
RDBSHNM H- KHAQ:QX OQDO:Q:SHNMR ENQ U:QHNTR OK:SENQLR

BZohkkZqx Rdptdmbhmf
0 OqdoZqhmf OkZrlhc Rtabknmdr enq BZohkkZqx Rdptdmbhmf 653
1 OqdoZqhmf OBQ Oqnctbsr enq BZohkkZqx Rdptdmbhmf 66/
2 Bxbkhmf Rdptdmbhmf QdZbshnmr 662
HkktlhmZ
3 Vgnkd Fdmnld9 LZmtZk KhaqZqx OqdoZqZshnm 665
4 Vgnkd Fdmnld9 ?tsnlZsdc) Mnmhmcdwdc KhaqZqx OqdoZqZshnm 672
?cchshnmZk Oqnsnbnk9 ?tsnlZsdc KhaqZqx OqdoZqZshnm 678
5 Vgnkd Fdmnld9 ?tsnlZsdc) Hmcdwdc KhaqZqx OqdoZqZshnm 681
6 OqdoZqZshnm ne Z 2,ja LZsd,OZhq KhaqZqx enq HkktlhmZ Rdptdmbhmf 688
7 OqdoZqZshnm ne Zm 7,ja LZsd,OZhq KhaqZqx enq HkktlhmZ Rdptdmbhmf 7/7
?cchshnmZk Oqnsnbnk9 ?LOtqd AdZc BZkhaqZshnm 710
8 QM?,Rdp9 QM? Bnmudqrhnm sn bCM? Zmc ?lokhehbZshnm 713
?cchshnmZk Oqnsnbnk9 QM?BkdZm WO AdZc BkdZmto %adenqd QM?,Rdp( 72/
0. Rnktshnm,OgZrd Dwnld BZostqd 720
?cchshnmZk Oqnsnbnk9 ?LOtqd WO AdZc BkdZmto 73/
?cchshnmZk Oqnsnbnk9 ?fZqnrd Fdk Rhyd Rdkdbshnm 731
00 ?tsnlZsdc Rhyd Rdkdbshnm 733
01 KhaqZqx PtZmshehbZshnm Trhmf RXAQ Fqddm,pOBQ 737
02 KhaqZqx PtZmshehbZshnm Trhmf OhbnFqddm Ektnqnldsqx 741
03 KhaqZqx PtZmshehbZshnm9 Ektnqnldsqhb PtZmshsZshnm ne Cntakd,RsqZmcdc nq
Rhmfkd,RsqZmcdc CM? RZlokdr Trhmf sgd Ptahs Rxrsdl 746
Oxqnrdptdmbhmf
04 OqdoZqZshnm ne RlZkk,EqZfldms KhaqZqhdr enq 343 Rdptdmbhmf 748
05 rrsCM? KhaqZqx BZostqd Zmc dlOBQ 755
06 Qnbgd 343 Rdptdmbdq9 Dwdbtshmf Z Rdptdmbhmf Qtm 763
RDBSHNM HH- ONRSRDPTDMBHMF :M:KXRDR

07 UZkhcZshnm 772
08 PtZkhsx ?rrdrrldms ne Rdptdmbd CZsZ 774
1. CZsZ ?mZkxrhr 776
HMENQL:SHNM O:MDKR
Ahnshm 777
LZfmdshb AdZcr 78/
EqZfldmshmf ne CM? 781
BG:OSDQ 01
:mYkxrhr ne CM: LdsgxkYshnm hm LYllYkhYm Bdkkr 782
OZtk L- KhyZqch Phm XZm Zmc MZqdmcqZ UZiZodxdd
HMSQNCTBSHNM
CM? LdsgxkZshnm ?eedbsr Zmc QdudZkr AhnknfhbZk OgdmnldmZ 783
DwodqhldmsZk ?ooqnZbgdr enq ?mZkxrhr ne CM? LdsgxkZshnm 784

whu Bnmsdmsr
?cuZmsZfdr Zmc KhlhsZshnmr ne Cheedqdms ?ooqnZbgdr enq ?mZkxyhmf CM? LdsgxkZshnm 787
Etstqd Odqrodbshudr 788
OQNSNBNKR
0 CM? Ahrtkehsd Rdptdmbhmf enq Rhmfkd,Mtbkdnshcd,Qdrnktshnm CM? LdsgxkZshnm
Cdsdbshnm 8//
1 LdsgxkZshnm,Rodbhehb OBQ enq Fdmd,Rodbhehb CM? LdsgxkZshnm Cdsdbshnm 8/6
2 Ldsgxk,Bxsnrhmd,AZrdc HlltmnoqdbhohsZshnm enq CM? LdsgxkZshnm ?mZkxrhr 800
3 Ghfg,Sgqntfgots Cddo Rdptdmbhmf enq LZoohmf LZllZkhZm CM? LdsgxkZshnm 804
4 Qnbgd 343 BknmZk Rdptdmbhmf ne Ahrtkehsd,Bnmudqsdc CM? KhaqZqhdr 816
5 HkktlhmZ Rdptdmbhmf ne Ahrtkehsd,Bnmudqsdc CM? KhaqZqhdr 821
HMENQL:SHNM O:MDKR
Otakhb CnlZhm RnesvZqd enq Hcdmshexhmf BoF HrkZmcr hm Oqnlnsdq Zmc Bnchmf Qdfhnmr
ne LZllZkhZm Fdmdr 826
Cdrhfmhmf Oqhldqr enq sgd ?lokhehbZshnm ne Ahrtkehsd,Bnmudqsdc Oqnctbs sn Odqenql
Ahrtkehsd Rdptdmbhmf Zmc LR,OBQ 828
Onrsrdptdmbd Oqnbdrrhmf ne Ghfg,Sgqntfgots Ahrtkehsd Cddo,Rdptdmbhmf CZsZ 83/
BG:OSDQ 02
OqdoYqYshnm ne KYadkdc CM:) QM:) Ymc Nkhfnmtbkdnshcd Oqnadr 832
HMSQNCTBSHNM
QZchnZbshud udqrtr MnmqZchnZbshud KZadkhmf ne Mtbkdhb ?bhcr 833
Sxodr ne MnmqZchnZbshud Cdsdbshnm Rxrsdlr 837
Cdrhfmhmf Nkhfnmtbkdnshcdr enq Trd Zr Oqnadr 842
OQNSNBNKR
0 QZmcnl Oqhlhmf9 KZadkhmf ne Otqhehdc CM? EqZfldmsr ax Dwsdmrhnm ne QZmcnl
Nkhfnmtbkdnshcdr 854
1 QZmcnl Oqhlhmf9 KZadkhmf ne CM? ax Dwsdmrhnm ne QZmcnl Nkhfnmtbkdnshcdr hm sgd
Oqdrdmbd ne Ldksdc ?fZqnrd 860
2 KZadkhmf ne CM? Oqnadr ax Mhbj SqZmrkZshnm 863
3 KZadkhmf ne CM? Oqnadr ax OnkxldqZrd BgZhm QdZbshnm 867
?cchshnmZk Oqnsnbnk9 ?rxlldsqhb Oqnadr 871
4 Rxmsgdrhr ne Rhmfkd,RsqZmcdc QM? Oqnadr ax Hm Uhsqn SqZmrbqhoshnm 873
?cchshnmZk Oqnsnbnk9 Trhmf OBQ sn ?cc Oqnlnsdqr enq AZbsdqhnogZfd,Dmbncdc
QM? OnkxldqZrdr sn EqZfldmsr ne CM? 880
5 Rxmsgdrhr ne bCM? Oqnadr eqnl lQM? Trhmf QZmcnl Nkhfnmtbkdnshcd Oqhldqr 883
6 QZchnkZadkhmf ne RtasqZbsdc bCM? Oqnadr ax QZmcnl Nkhfnmtbkdnshcd Dwsdmrhnm 886
7 KZadkhmf 2 Sdqlhmh ne Cntakd,RsqZmcdc CM? Trhmf sgd Jkdmnv EqZfldms ne
D- bnkh CM? OnkxldqZrd H 0//2
8 CdognrognqxkZshnm ne CM? EqZfldmsr vhsg ?kjZkhmd OgnrogZsZrd 0//8
0. OgnrognqxkZshnm ne CM? Lnkdbtkdr vhsg Oqnsqtchmf 4 ,Gxcqnwxk Sdqlhmh 0/01

Bnmsdmsr wu
00 OgnrognqxkZshnm ne CM? Lnkdbtkdr vhsg CdognrognqxkZsdc Aktms Dmcr nq

Qdbdrrdc 4 Sdqlhmh 0/04
01 OgnrognqxkZshmf sgd 4 Sdqlhmh ne Nkhfnmtbkdnshcdr Trhmf S3 Onkxmtbkdnshcd JhmZrd 0/07
02 KZadkhmf sgd 2 Sdqlhmh ne Nkhfnmtbkdnshcdr Trhmf SdqlhmZk Cdnwxmtbkdnshcxk
SqZmredqZrd 0/10
?ksdqmZshud Oqnsnbnk9 Rxmsgdrhyhmf MnmqZchnkZadkdc Oqnadr Trhmf ScS 0/12
?cchshnmZk Oqnsnbnk9 SZhkhmf QdZbshnm 0/13
?cchshnmZk Oqnsnbnk9 LnchehbZshnmr enq Rxmsgdrhyhmf MnmqZchnkZadkdc Oqnadr 0/15
03 KZadkhmf ne Rxmsgdshb Nkhfnmtbkdnshcdr Trhmf sgd Jkdmnv EqZfldms ne D- bnkh CM?
OnkxldqZrd H 0/17
04 OtqhehbZshnm ne KZadkdc Nkhfnmtbkdnshcdr ax OqdbhohsZshnm vhsg DsgZmnk 0/21
05 OtqhehbZshnm ne KZadkdc Nkhfnmtbkdnshcdr ax Rhyd,Dwbktrhnm BgqnlZsnfqZogx 0/23
06 OtqhehbZshnm ne KZadkdc Nkhfnmtbkdnshcdr ax BgqnlZsnfqZogx nm Z Rdo,OZj B07 Bnktlm 0/26
07 GxaqhchyZshnm ne Nkhfnmtbkdnshcd Oqnadr hm ?ptdntr Rnktshnmr9 VZrghmf hm Ateedqr
BnmsZhmhmf PtZsdqmZqx ?llnmhtl RZksr 0/28
HMENQL:SHNM O:MDKR
OqdoZqZshnm ne Rsnbj Rnktshnmr ne cMSOr 0/32
D- bnkh CM? OnkxldqZrd H Zmc sgd Jkdmnv EqZfldms 0/33
Hm Uhsqn SqZmrbqhoshnm Rxrsdlr 0/4/
?kjZkhmd OgnrogZsZrd 0/42
Ldkshmf SdlodqZstqdr 0/44
BG:OSDQ 03
Ldsgncr enq Hm Uhsqn LtsYfdmdrhr 0.48
LZssdn Enqknmh :kdv X- Kht Zmc MZqdmcqZ UZiZodxdd
HMSQNCTBSHNM
LtsZfdmdrhr ?ooqnZbgdr 0/53
QdrdZqbg FnZkr 0/54
BnlldqbhZk Jhsr 0/54
OQNSNBNKR
0 QZmcnl LtsZfdmdrhr Trhmf Dqqnq,Oqnmd CM? OnkxldqZrdr 0/57
1 BqdZshmf Hmrdqshnmr nq Cdkdshnmr Trhmf NudqkZo Dwsdmrhnm OBQ LtsZfdmdrhr 0/7/
2 Hm Uhsqn LtsZfdmdrhr Trhmf Cntakd,RsqZmcdc CM? SdlokZsdr9 Rdkdbshnm
ne LtsZmsr vhsg ComH 0/76
3 ?ksdqdc a,KZbsZlZrd Rdkdbshnm ?ooqnZbg enq Rhsd,Chqdbsdc LtsZfdmdrhr 0/84
4 Nkhfnmtbkdnshcd,Chqdbsdc LtsZfdmdrhr ax DkhlhmZshnm ne Z Tmhptd Qdrsqhbshnm Rhsd
%TRD LtsZfdmdrhr( 00/1
5 RZstqZshnm LtsZfdmdrhr ax Bncnm BZrrdssd Hmrdqshnm 00/7
6 QZmcnl RbZmmhmf LtsZfdmdrhr 0004
7 Ltkshrhsd,Chqdbsdc LtsZfdmdrhr 001/
8 LdfZoqhldq OBQ,AZrdc LtsZfdmdrhr 0013

wuh Bnmsdmsr
HMENQL:SHNM O:MDKR
CnlZhm LtsZfdmdrhr 0016
Ghfg,Sgqntfgots Rhsd,Chqdbsdc LtsZfdmdrhr ne OkZrlhc CM? 0017
5
M ,LdsgxkZcdmhmd) CZl LdsgxkZrd) Zmc LdsgxkZshnm,Rdmrhshud
Qdrsqhbshnm Dmyxldr 0018
O:QS 29 HMSQNCTBHMF FDMDR HMSN BDKKR
BG:OSDQ 04
Hmsqnctbhmf Fdmdr hmsn Btkstqdc LYllYkhYm Bdkkr 0020
Oqhsh JtlZq :quhmcgZm MZfZqZiZm Zmc OqZcddo C- Tbghk
HMSQNCTBSHNM
SqZmrhdms Udqrtr RsZakd SqZmredbshnm 0022
SqZmredbshnm Ldsgncr 0022
SqZmredbshnm Bnmsqnkr 0022
NoshlhyZshnm Zmc RodbhZk BnmrhcdqZshnmr 0025
?rrdrrhmf Bdkk UhZahkhsx hm SqZmredbsdc Bdkk Khmdr 0026
OQNSNBNKR
0 CM? SqZmredbshnm LdchZsdc ax BZshnmhb Khohc QdZfdmsr 0028
?ksdqmZshud Oqnsnbnk9 SqZmredbshnm Trhmf CNSL? Zmc CNFR 0034
?cchshnmZk Oqnsnbnk9 GhrsnbgdlhbZk RsZhmhmf ne Bdkk LnmnkZxdqr enq a,FZkZbsnrhcZrd 0037
1 BZkbhtl,OgnrogZsd,LdchZsdc SqZmredbshnm ne DtjZqxnshb Bdkkr vhsg OkZrlhc CM?r 004/
?ksdqmZshud Oqnsnbnk9 Ghfg,Deehbhdmbx BZkbhtl,OgnrogZsd,LdchZsdc SqZmredbshnm ne
DtjZqxnshb Bdkkr vhsg OkZrlhc CM?r 0045
2 BZkbhtl,OgnrogZsd,LdchZsdc SqZmredbshnm ne Bdkkr vhsg Ghfg,LnkdbtkZq,Vdhfgs
Fdmnlhb CM? 005/
?ksdqmZshud Oqnsnbnk9 BZkbhtl,OgnrogZsd,LdchZsdc SqZmredbshnm ne ?cgdqdms Bdkkr 0052
?ksdqmZshud Oqnsnbnk9 BZkbhtl,OgnrogZsd,LdchZsdc SqZmredbshnm ne Bdkkr Fqnvhmf
hm Rtrodmrhnm 0054
3 SqZmredbshnm LdchZsdc ax CD?D,CdwsqZm9 Ghfg,Deehbhdmbx Ldsgnc 0056
?ksdqmZshud Oqnsnbnk9 SqZmredbshnm LdchZsdc ax CD?D,CdwsqZm9 HmbqdZrdc
Bdkk UhZahkhsx 006/
4 CM? SqZmredbshnm ax DkdbsqnonqZshnm 0062
5 ?mZkxrhr ne Bdkk UhZahkhsx ax sgd ZkZlZqAktd ?rrZx 0066
6 ?mZkxrhr ne Bdkk UhZahkhsx ax sgd KZbsZsd CdgxcqnfdmZrd ?rrZx 007/
7 ?mZkxrhr ne Bdkk UhZahkhsx ax sgd LSS ?rrZx 0072
HMENQL:SHNM O:MDKR
NoshbZk SqZmredbshnm 0075
BnsqZmrenqlZshnm 0077
Rdkdbshud ?fdmsr enq RsZakd SqZmrenqlZshnm 008/
Khonedbshnm 0083

Bnmsdmsr wuhh
KhmdZqhyhmf OkZrlhcr adenqd SqZmredbshnm 0086

SqZmredbshnm ne LZllZkhZm Bdkkr vhsg BZkbhtl OgnrogZsd “CM? BnoqdbhohsZsdr 0087
Bgknqnpthmd ChognrogZsd 01//
CD?D,CdwsqZm SqZmredbshnm 01/0
DkdbsqnonqZshnm 01/2
BG:OSDQ 05
Hmsqnctbhmf Fdmdr hmsn LYllYkhYm Bdkkr9 UhqYk Udbsnqr 01.8
FtZmfohmf FZn Zmc Lhftdk RdmZ,Drsdudr
HMSQNCTBSHNM
EZbsnqr sn Bnmrhcdq Vgdm Bgnnrhmf Z UhqZk Udbsnq 0100
Sgd LZinq Sxodr ne Uhqtrdr Btqqdmskx Trdc Zr Udbsnqr 0101
Hm Uhun Dwoqdrrhnm 0110
?cdmnuhqtr Udbsnqr 0110
?cdmn,?rrnbhZsdc Uhqtr Udbsnqr 0113
Qdsqnuhqtr Zmc Kdmshuhqtr Udbsnqr 0116
OQNSNBNKR
0 Bnmrsqtbshnm ne QdbnlahmZms ?cdmnuhqtr Fdmnldr ax Chqdbs Bknmhmf 0122
1 QdkdZrd ne sgd Bknmdc QdbnlahmZms ?cdmnuhqtr Fdmnld enq Qdrbtd Zmc DwoZmrhnm 0127
2 OtqhehbZshnm ne sgd QdbnlahmZms ?cdmnuhqtr ax Bdrhtl Bgknqhcd FqZchdms
BdmsqhetfZshnm 0133
3 BgZqZbsdqhyZshnm ne sgd Otqhehdc QdbnlahmZms ?cdmnuhqtr Udbsnq enq UhqZk Fdmnld
Rsqtbstqd ax Qdrsqhbshnm Dmyxld Chfdrshnmr 0138
4 LdZrtqhmf sgd Hmedbshntr Shsdq ne QdbnlahmZms ?cdmnuhqtr Udbsnqr Trhmf
SBHC4/ Dmc,Onhms Chktshnm Zmc pOBQ 0142
?cchshnmZk Oqnsnbnk9 OqdoZqZshnm ne Z CM? RsZmcZqc enq pOBQ 0151
5 Cdsdbshnm ?rrZx enq QdokhbZshnm,Bnlodsdms ?cdmnuhqtr ax BnmbdmsqZshnm
OZrrZfd Zmc QdZk,Shld pOBQ 0153
6 Oqnctbshnm ne q??Ur ax SqZmrhdms SqZmredbshnm 0163
7 OtqhehbZshnm ne q??Ur ax Bdrhtl Bgknqhcd FqZchdms RdchldmsZshnm 0167
8 OtqhehbZshnm ne q??Ur ax HnchwZmnk FqZchdms BdmsqhetfZshnm 0172
0. OtqhehbZshnm ne q??U1r ax GdoZqhm Bnktlm ?eehmhsx BgqnlZsnfqZogx 0176
00 Dmqhbgldms ne Etkkx OZbjZfdc Uhqhnmr hm Bnktlm,Otqhehdc q??U OqdoZqZshnmr
ax HnchwZmnk FqZchdms BdmsqhetfZshnm Enkknvdc ax ?mhnm,DwbgZmfd Bnktlm
BgqnlZsnfqZogx 018/
01 ShsqZshnm ne q??U Fdmnld Bnox Mtladq Trhmf QdZk,Shld pOBQ 0183
02 Rdmrhshud CdsdqlhmZshnm ne Hmedbshntr Shsdq ne q??Ur Trhmf SBHC4/
Dmc,Onhms Chktshnm Zmc pOBQ 0187
03 ?mZkxrhr ne q??U RZlokd Lnqognknfx Trhmf MdfZshud RsZhmhmf Zmc
Ghfg,Qdrnktshnm Dkdbsqnm Lhbqnrbnox 02/0
04 ?mZkxrhr ne q??U Otqhsx Trhmf Rhkudq,RsZhmdc RCR,O?FD 02/3
05 Oqnctbshnm ne Ghfg,Shsdq Qdsqnuhqtr Zmc Kdmshuhqtr Udbsnqr 02/6

wuhhh Bnmsdmsr
06 ShsqZshnm ne Kdmshuhqtr Udbsnqr 0203

07 Lnmhsnqhmf Kdmshuhqtr Udbsnq Rsnbjr enq QdokhbZshnm,Bnlodsdms Uhqtrdr 0208
HMENQL:SHNM O:MDKR
?cdmnuhqtr Udbsnqr 0211
??U Udbsnqr 0212
Kdmshuhqtr Udbsnqr 0213
AZrhb Dkdldmsr hm UhqZk Udbsnqr 0215
?rrZxr Cnmd hm SqZmrctbdc Bdkkr 0217
SqZmrfdmd Dwoqdrrhnm BZrrdssdr 022/
UNKTLD 2
O:QS 39 FDMD DWOQDRRHNM
BG:OSDQ 06
:mYkxrhr ne Fdmd QdftkYshnm Trhmf Qdonqsdq Rxrsdlr 0224
OqZcddo C- Tbghk :quhmcgZm MZfZqZiZm Zmc Oqhsh JtlZq
HMSQNCTBSHNM
Hmsqnctbshnm sn Qdonqsdq Rxrsdlr 0225
Qdonqsdq Fdmdr Trdc hm sgd ?mZkxrhr ne QdftkZsnqx Dkdldmsr 0225
?rrZxhmf enq a,FZkZbsnrhcZrd hm DwsqZbsr ne LZllZkhZm Bdkkr 0227
?rrZxhmf enq KtbhedqZrd hm DwsqZbsr ne LZllZkhZm Bdkkr 0228
SdsqZbxbkhmd,Qdronmrhud Dwoqdrrhnm Rxrsdlr 0230
OQNSNBNKR
0 ?rrZx enq a,FZkZbsnrhcZrd hm DwsqZbsr ne LZllZkhZm Bdkkr 0235
?cchshnmZk Oqnsnbnk9 Bgdlhktlhmdrbdms ?rrZx enq a,FZkZbsnrhcZrd ?bshuhsx 024/
1 Rhmfkd KtbhedqZrd Qdonqsdq ?rrZx 0243
2 CtZk KtbhedqZrd Qdonqsdq ?rrZx 0248
3 Trhmf DKHR? sn LdZrtqd FEO Oqnctbshnm 0255
4 FdmdqZshnm ne Bdkk Khmdr vhsg SdsqZbxbkhmd,QdftkZsdc Fdmd Dwoqdrrhnm 026/
?cchshnmZk Oqnsnbnk9 Rdkdbshmf RsZakd Bknmdr uhZ Khlhshmf Chktshnm
ne Rtrodmrhnm Bdkkr 0267
HMENQL:SHNM O:MDKR
Ektnqdrbdms Oqnsdhmr 0270
Dohsnod SZffhmf 0283
a,FZkZbsnrhcZrd 03/0
KtbhedqZrd 03/5
SdsqZbxbkhmd 03/8

Bnmsdmsr whw
BG:OSDQ 07
QM: Hmsdqedqdmbd Ymc RlYkk QM: :mYkxrhr 0304
BgdmfihZm Kh Zmc Oghkkho C- YZlnqd
HMSQNCTBSHNM
Qdudqrd Fdmdshbr ax QM?h 0308
?mZkxrhr ne RlZkk QM?r 0314
OQNSNBNKR
0 OqdoZqZshnm ne rhQM? Ctokdwdr 0320
1 QM?h hm LZllZkhZm Bdkkr ax rhQM? Ctokdw SqZmredbshnm 0323
2 QM?h hm CqnrnoghkZ R1 Bdkkr ax rhQM? Ctokdw SqZmredbshnm 0326
3 OqdoZqZshnm ne crQM?r ax Hm Uhsqn SqZmrbqhoshnm 033/
4 QM?h hm CqnrnoghkZ R1 Bdkkr ax crQM? RnZjhmf 0334
5 QM?h hm CqnrnoghkZ R1 Bdkkr ax crQM? SqZmredbshnm 0336
6 ?mZkxrhr ne RlZkk QM?r ax Mnqsgdqm GxaqhchyZshnm 0337
7 ?mZkxrhr ne RlZkk QM?r ax PtZmshsZshud Qdudqrd SqZmrbqhoshnm OBQ 0342
8 Bnmrsqtbshnm ne RlZkk QM? KhaqZqhdr enq Ghfg,Sgqntfgots Rdptdmbhmf 0345
0. OqdoZqZshnm ne ?mshrdmrd Nkhfnmtbkdnshcdr sn Hmghahs lhQM? Etmbshnm 0355
00 Hmghahshmf lhQM? Etmbshnm ax ?mshrdmrd Nkhfnmtbkdnshcdr hm Btkstqdc LZllZkhZm Bdkkr 0357
01 Hmghahshmf lhQM? Etmbshnm ax ?mshrdmrd Nkhfnmtbkdnshcdr hm CqnrnoghkZ R1 Bdkkr 036/
HMENQL:SHNM O:MDK
Fdmnld,Vhcd QM? Hmsdqedqdmbd9 EtmbshnmZk Fdmnlhbr hm sgd Onrsfdmnlhbr DqZ 0361
RsZqEhqd Oqnadr 0367
BG:OSDQ 08
Dwoqdrrhmf Bknmdc Fdmdr enq Oqnsdhm Oqnctbshnm) OtqhehbYshnm) Ymc :mYkxrhr 0370
BkZqZ K- Jhdkjnoe UhkkhZl AZtdq Zmc HmZ K- TqaZsrbg
HMSQNCTBSHNM
Bgnnrhmf Zm Dwoqdrrhnm Rxrsdl 0372
Bgnnrhmf Zm ?ooqnoqhZsd Dwoqdrrhnm Udbsnq 0377
Etrhnm Oqnsdhmr 0388
NoshlhyZshnm ne Dwoqdrrhnm ne Enqdhfm Oqnsdhmr 04/2
OQNSNBNKR
0 Dwoqdrrhnm ne Bknmdc Fdmdr hm D- bnkh Trhmf HOSF,Hmctbhakd Oqnlnsdqr 04/7
?cchshnmZk Oqnsnbnk9 RlZkk,RbZkd Sdrs enq Rnktakd SZqfds Oqnsdhm Dwoqdrrhnm 0403
?ksdqmZshud Oqnsnbnk9 Dwoqdrrhnm ne Bknmdc Fdmdr hm D- bnkh Trhmf sgd ?qZahmnrd
A:C Oqnlnsdq 041/
?ksdqmZshud Oqnsnbnk9 RtabdkktkZq KnbZkhyZshnm ne RhfmZk Odoshcd Etrhnm Oqnsdhmr 0411

ww Bnmsdmsr
1 Dwoqdrrhnm ne Bknmdc Fdmdr Trhmf sgd AZbtknuhqtr Dwoqdrrhnm Rxrsdl 0416

?cchshnmZk Oqnsnbnk9 OkZptd ?rrZx sn Cdsdqlhmd sgd Shsdq ne sgd AZbtknuhqtr Rsnbj 0424
?ksdqmZshud Oqnsnbnk9 Oqnctbshnm ne AZblhc CM? enq SqZmredbshnm hmsn
Hmrdbs Bdkkr 0427
2 Dwoqdrrhnm ne Bknmdc Fdmdr hm O- oZrsnqhr Trhmf sgd LdsgZmnk,Hmctbhakd
Oqnlnsdq :NW. 0431
?cchshnmZk Oqnsnbnk9 BqxnrsnqZfd ne XdZrs Btkstqdr 0442
3 OqdoZqZshnm ne Bdkk DwsqZbs enq OtqhehbZshnm ne Rnktakd Oqnsdhmr Dwoqdrrdc hm D- bnkh 0447
?cchshnmZk Oqnsnbnk9 Kxrhr ne XdZrs Bdkkr Trhmf FkZrr AdZcr 0453
?ksdqmZshud Oqnsnbnk9 OqdoZqZshnm ne D- bnkh Bdkk DwsqZbs Trhmf Fdmskd)
GdZs,Hmctbdc DmyxlZshb Kxrhr 0455
?ksdqmZshud Oqnsnbnk9 OqdoZqZshnm ne D- bnkh Bdkk DwsqZbs Trhmf Eqddyd “SgZv
vhsg DmyxlZshb Kxrhr ax Kxrnyxld 0457
4 OtqhehbZshnm ne Onkxghrshchmd,SZffdc Oqnsdhmr ax Hllnahkhydc LdsZk ?eehmhsx
BgqnlZsnfqZogx 0460
?cchshnmZk Oqnsnbnk9 QdfdmdqZshmf Zmc BkdZmhmf sgd Mh10 “ MS? Qdrhm 0468
?ksdqmZshud Oqnsnbnk9 EZrs OdqenqlZmbd Khpthc BgqnlZsnfqZogx OtqhehbZshnm
ne Ghrshchmd,SZffdc Oqnsdhmr 0470
5 OtqhehbZshnm ne Etrhnm Oqnsdhmr ax ?eehmhsx BgqnlZsnfqZogx nm FktsZsghnmd Qdrhm 0475
6 RnktahkhyZshnm ne Dwoqdrrdc Oqnsdhmr eqnl Hmbktrhnm Anchdr 0482
7 RCR,O?FD ne Oqnsdhmr 0488
?ksdqmZshud Oqnsnbnk9 UZqhZshnmr ne RsZhmhmf RCR “ OnkxZbqxkZlhcd Fdkr vhsg
BnnlZrrhd AqhkkhZms Aktd 05/8
?ksdqmZshud Oqnsnbnk9 RsZhmhmf RCR “OnkxZbqxkZlhcd Fdkr vhsg Rhkudq RZksr 0500
8 ?mZkxrhr ne Oqnsdhmr ax Hlltmnaknsshmf 0505
0. Ldsgncr enq LdZrtqhmf sgd BnmbdmsqZshnmr ne Oqnsdhmr 0514
HMENQL:SHNM O:MDKR
BnmrhcdqZshnmr enq LdlaqZmd Oqnsdhm OtqhehbZshnm 0521
GhrsnqhbZk Ennsmnsd9 BnnlZrrhd AqhkkhZms Aktd 0525
O:QS 49 HMSDQ:BSHNM :M:KXRHR

BG:OSDQ 1.
Bqnrr,Khmihmf Sdbgmnknfhdr enq :mYkxrhr ne BgqnlYshm Rsqtbstqd Ymc Etmbshnm 0526
SZd Gnnm Jhl Zmc Ina Cdjjdq
HMSQNCTBSHNM
EnqlZkcdgxcd Bqnrr,Khmjhmf sn HmsdqqnfZsd Fdmnlhb HmsdqZbshnmr 0527
BgHO ?mZkxrhr ne Oqnsdhm “CM? HmsdqZbshnmr 0527
2B,AZrdc BgqnlZshm HmsdqZbshnm ?mZkxrdr 0530
OQNSNBNKR
0 EnqlZkcdgxcd Bqnrr,Khmjhmf 0538
1 OqdoZqZshnm ne Bqnrr,Khmjdc BgqnlZshm enq BgHO 0541

Bnmsdmsr wwh
2 BgHO 0544
3 BgHO“ PtZmshsZshud OBQ %BgHO,pOBQ( 0548
4 BgHO,bgho 0550
5 BgHO,rdp 0558
6 FdmdqZshnm ne 2B KhaqZqhdr eqnl Bqnrr,Khmjdc Bdkkr 0563
7 FdmdqZshnm ne BgHO,knno KhaqZqhdr 0568
8 FdmdqZshnm ne Bnmsqnk KhfZshnm Oqnctbs KhaqZqhdr 0573
0. OBQ Cdsdbshnm ne 2B KhfZshnm Oqnctbsr Oqdrdms hm 2B) BgHO,knno) Zmc
Bnmsqnk KhaqZqhdr9 KhaqZqx ShsqZshnm Zmc HmsdqZbshnm Eqdptdmbx ?mZkxrhr 0576
00 3B ?mZkxrhr ne 2B) BgHO,knno) Zmc Bnmsqnk KhaqZqhdr 0581
01 4B ?mZkxrhr ne 2B) BgHO,knno) Zmc Bnmsqnk KhaqZqhdr 0585
HMENQL:SHNM O:MDKR
EnqlZkcdgxcd 06/0
VgZs Hr BZostqdc ax 2B,AZrdc ?rrZxr: 06/1
BG:OSDQ 10
LYoohmf ne Hm Uhun QM:,Ahmchmf Rhsdr ax TU,Bqnrr,Khmihmf
HlltmnoqdbhohsYshnm BKHO( 06.2
Idmmhedq B- CZqmdkk :kcn Ldkd JZ Xhmf RgZqnm Gtmf Zmc Qnadqs A- CZqmdkk
HMSQNCTBSHNM
Sgd Bqnrr,Khmjhmf HlltmnoqdbhohsZshnm Ldsgnc 06/5
Ghfg,Sgqntfgots Rdptdmbhmf %GHSR( BKHO 06/7
UZkhcZshnm ne BKHO Qdrtksr 06/7
BKHO Ldsgnc UZqhZshnmr 06/8
FdmdqZk BnmrhcdqZshnmr hm OkZmmhmf BKHO Dwodqhldmsr 060/
OQNSNBNKR
0 NoshlhyZshnm ne HlltmnoqdbhohsZshnm Rsqhmfdmbx enq BKHO 0602
1 TU Bqnrr,Khmjhmf ne Khud Bdkkr Zmc KxrZsd OqdoZqZshnm 061/
2 QMZrd ShsqZshnm) HlltmnoqdbhohsZshnm) Zmc RCR,O?FD 0613
3 2 ,Khmjdq KhfZshnm Zmc Rhyd Rdkdbshnm ax RCR,O?FD 0623
?ksdqmZshud Oqnsnbnk9 4 ,Dmc KZadkhmf ne CdognrognqxkZsdc QK2 Khmjdq 0627
4 HrnkZshnm ne sgd QM? SZfr) 4 ,Khmjdq KhfZshnm) Zmc Qdudqrd SqZmrbqhoshnm
OBQ ?lokhehbZshnm 0630
5 Rdptdmbhmf ne QM? BKHO SZfr 0640
6 Fdk OtqhehbZshnm Zmc RsnqZfd ne QM? Khmjdqr 0642
HMENQL:SHNM O:MDKR
LdbgZmhrl Zmc Rodbhehbhsx ne TU,Oqnsdhm Bqnrr,Khmjhmf 0645
GHSR,BKHO CZsZ ?mZkxrhr 0647

wwhh Bnmsdmsr
BG:OSDQ 11
FYsdvYx,BnloYshakd XdYrs Nmd,Gxaqhc Ymc Svn,Gxaqhc :rrYxr 0650
Ingm R- Qddbd,Gnxdr Zmc :kadqsgZ I-L- UZkgnts
HMSQNCTBSHNM
Sgd XdZrs Svn,Gxaqhc %X1G( Rxrsdl9 Bnmbdos Zmc Ldsgncnknfx 0652
Sgd XdZrs Nmd,Gxaqhc %X0G( Rxrsdl9 Bnmbdos Zmc Ldsgncnknfx 0656
X1G Zmc X0G ?rrZxr9 ?cuZmsZfdr Zmc ChrZcuZmsZfdr 0657
EZkrd Onrhshudr 0658
Oqnsnbnkr enq XdZrs Nmd,Gxaqhc Zmc Svn,Gxaqhc Rxrsdlr 066/
OQNSNBNKR
0 FdmdqZshmf XdZrs Nmd,Gxaqhc CM?,AZhs RsqZhmr 0662
?ksdqmZshud Oqnsnbnk9 BqdZshmf Dmsqx Bknmdr eqnl CM?,AZhsr FdmdqZsdc ax
?mmdZkhmf Oqhldqr 0671
1 FdmdqZshmf XdZrs Svn,Gxaqhc AZhs RsqZhmr 0674
2 Hcdmshexhmf HmsdqZbsnqr eqnl Zm ?bshuZshnm CnlZhm Oqdx KhaqZqx 0681
3 Ghfg,Deehbhdmbx XdZrs SqZmrenqlZshnm 0688
4 Bnknmx Khes Bnknqhldsqhb ?rrZx enq a,FZkZbsnrhcZrd ?bshuhsx 07/2
5 XdZrs Bnknmx OBQ 07/4
HMENQL:SHNM O:MDKR
Vgx HmsdfqZsd CM?,AZhsr: 07/7
Bgnnrhmf Z Udbsnq Zmc Z XdZrs RsqZhm 07/8
QdokhbZ,OkZshmf Zmc QdokhbZ,BkdZmhmf Trhmf Udkudsr 070/
:OODMCHBDR
:OODMCHW 0
QdYfdmsr Ymc Ateedqr 0700
QDBHODR 0700
ATEEDQR 0717
Sqhr Ateedqr 0717
Fnnc Ateedqr 0718
OgnrogZsd Ateedqr %Fnlnqh Ateedqr( 072/
:BHCR :MC A:RDR 0722
NQF:MHB QD:FDMSR 0723

Ogdmnk 0723
DpthkhaqZshnm ne Ogdmnk 0723
Ogdmnk9Bgknqnenql9HrnZlxk ?kbngnk %1491390( 0723
CdhnmhyZshnm ne EnqlZlhcd 0723

Bnmsdmsr wwhhh
:MSHAHNSHBR 0724
AKNBIHMF :FDMSR 0725

Aknbjhmf ?fdmsr Trdc enq Mtbkdhb ?bhc GxaqhchyZshnm 0725
Aknbjhmf ?fdmsr Trdc enq Vdrsdqm Aknsshmf 0725
A:BSDQH:K RSNQ:FD LDCH: 0728
ODQHNCHB S:AKD 073/
QD:FDMSR :MC ATEEDQR HMCDW 0730
:OODMCHW 1
Bnllnmkx Trdc Sdbgmhptdr 0732
OQDO:Q:SHNM NE FK:RRV:QD :MC OK:RSHBV:QD 0732

Rhkhbnmhyhmf FkZrrvZqd) OkZrshbvZqd) Zmc FkZrr Vnnk 0732
OqdoZqZshnm ne QMZrd,Eqdd FkZrrvZqd 0733
OQDO:Q:SHNM NE CH:KXRHR STAHMF 0734
DRSHL:SHNM NE BDKK MTLADQ 0735

Gdlnbxsnldsqx Bntmshmf 0735
UhZahkhsx RsZhmhmf 0736
LD:RTQDLDMS NE Q:CHN:BSHUHSX HM MTBKDHB :BHCR 0738

OqdbhohsZshnm ne Mtbkdhb ?bhcr vhsg SqhbgknqnZbdshb ?bhc 0738
CDBNMS:LHM:SHNM NE RNKTSHNMR BNMS:HMHMF DSGHCHTL AQNLHCD 0740

Qdlnuhmf Dsghchtl Aqnlhcd eqnl CM? 0740
Chronrhmf ne Dsghchtl Aqnlhcd 0740
CdbnmsZlhmZshnm ne BnmbdmsqZsdc Rnktshnmr ne Dsghchtl Aqnlhcd %Rnktshnmr
BnmsZhmhmf ,/-4 lf.lK( 0740
CdbnmsZlhmZshnm ne Chktsd Rnktshnmr ne Dsghchtl Aqnlhcd %d-f-) Dkdbsqnognqdrhr Ateedq
BnmsZhmhmf /-4 lf.lK Dsghchtl Aqnlhcd( 0741
BnlldqbhZk CdbnmsZlhmZshnm Jhsr 0741
:OODMCHW 2
Cdsdbshnm Rxrsdlr 0744
RS:HMHMF MTBKDHB :BHCR 0744

Dsghchtl Aqnlhcd 0744
Ldsgxkdmd Aktd 0746
RXAQ Cxdr 0746
BGDLHKTLHMDRBDMBD 0748
Bgdlhktlhmdrbdms KZadkr 075/
Bgdlhktlhmdrbdms Dmyxld ?rrZxr 0750
BnlldqbhZk QdZfdmsr) Jhsr) Zmc Ktlhmnldsdqr 0752

wwhu Bnmsdmsr
HMENQL:SHNM O:MDKR
GnqrdqZchrg OdqnwhcZrd 0754
Chfnwxfdmhm 0758
ABHO 0762
?LOOC 0765
Hlltmnfknatkhm,Ahmchmf Oqnsdhmr9 Oqnsdhmr ?) F) Zmc K 0768
:OODMCHW 3
FdmdqYk RYedsx Ymc GYyYqcntr LYsdqhYk 0774
Hmcdw H,0

Ihrs ne SYakdr
BG:OSDQ 0
HrnkYshnm Ymc PtYmshehbYshnm ne CM:
BZshnmr bnllnmkx trdc hm dsgZmnk oqdbhohsZshnm ne CM? 11
BZqqhdqr trdc hm dsgZmnk oqdbhohsZshnm 12
Mtbkdnshcd btsneer enq Lhbqnbnm bnmbdmsqZsnqr 17
SZakd rsqZhmr ne DrbgdqhbghZ bnkh bnllnmkx trdc Zr gnrsr enq aZbsdqhnogZfd L02 24
Oqnsdnkxshb dmyxldr 33
RMDS kxrhr ateedq unktldr 48
BG:OSDQ 1
:mYkxrhr ne CM:
Oqnodqshdr ne cheedqdms sxodr ne ZfZqnrdr 73
QZmfd ne rdoZqZshnm ne CM? eqZfldmsr sgqntfg cheedqdms sxodr ne ZfZqnrdr 73
Oqnodqshdr ne ldlaqZmdr trdc enq Rntsgdqm aknsshmf Zmc gxaqhchyZshnm 81
QZmfd ne rdoZqZshnm hm fdkr bnmsZhmhmf cheedqdms Zlntmsr ne rsZmcZqc
knv,DDN ZfZqnrd 85
Deedbshud qZmfd ne rdoZqZshnm ne CM?r hm onkxZbqxkZlhcd fdkr 0/5
Unktldr ne qdZfdmsr trdc sn bZrs onkxZbqxkZlhcd fdkr 0/6
Rdmrhshuhsx ne ZtsnqZchnfqZoghb ldsgncr enq cdsdbshnm ne qZchnhrnsnodr 013
Ehwhmf CM? sn sgd ldlaqZmd enq gxaqhchyZshnm 026
AZbjfqntmc”Gnv sn Zunhc hs 038
BG:OSDQ 2
Bknmhmf Ymc SqYmrenqlYshnm vhsg OkYrlhc Udbsnqr
BnlldqbhZk jhsr enq sqZmrenqlZshnm ne DrbgdqhbghZ bnkh 050
Bnmchshnmr enq cdognrognqxkZshnm ne 4 qdrhctdr eqnl CM? 08/
Bnlonmdmsr enq sno ZfZq 100
D- bnkh”? shldkhmd 103
RsZmcZqc ldsgncr enq bknmhmf Zlokhehdc CM? 108
Oqnodqshdr ne sgd FZsdvZx,lnchehdc Zss rhsdr 11/
KhfZrd,eqdd ldsgncr 112
CM? khfZrdr 125
RtllZqx9 LZinq bkZrrdr ne qdrsqhbshnm dmyxldr 131
OkZrlhc qdokhbnmr Zmc lncdr ne qdokhbZshnm 140
Bnmsqnk dkdldmsr sgZs qdftkZsd qdokhbZshnm 141
Snwhb fdmdr trdc hm onrhshud,rdkdbshnm rxrsdlr 144
wwu

wwuh Khrs ne SZakdr
BG:OSDQ 3
FYsdvYx QdbnlahmYshnmYk Bknmhmf
?cuZmsZfdr Zmc chrZcuZmsZfdr ne cheedqdms qdbnlahmZshnm,aZrdc bknmhmf ldsgncr 154
BG:OSDQ 4
Vnqihmf vhsg AYbsdqhYk :qshehbhYk Bgqnlnrnldr Ymc Nsgdq Ghfg,BYoYbhsx Udbsnqr
Ghfg,bZoZbhsx udbsnqr enq fdmnlhb bknmhmf 171
Oqhldqr qdpthqdc enq rdkdbshmf bnhmsdfqZsdr 2/6
BG:OSDQ 5
DwsqYbshnm) OtqhehbYshnm) Ymc :mYkxrhr ne QM: eqnl DtiYqxnshb Bdkkr
BnlldqbhZkkx ZuZhkZakd lnmnogZrhb kxrhr qdZfdmsr 238
Mtladq ne kZquZd Zmc Zctks vnqlr qdpthqdc enq snsZk QM? dwsqZbshnm 25/
Nudquhdv ne rodbsqnognsnldsqhb Zmc ektnqdrbdms,aZrdc cxd ldsgncr 255
RZks rnktshnmr enq oqdbhohsZshmf QM? 262
BnlldqbhZkkx ZuZhkZakd nkhfn%cS(,lZfmdshb adZcr 267
Oqnodqshdr ne mxknm ldlaqZmdr trdc enq hllnahkhyZshnm ne CM? Zmc QM? 273
OdqbdmsZfd ne ZbqxkZlhcd enq deedbshud rdoZqZshnm ne QM?r 283
Qdbhodr enq 7 L tqdZ cdmZstqhmf ZbqxkZlhcd fdkr 286
Dwodbsdc lnahkhshdr ne sqZbjhmf cxdr vgdm qtm vhsg QM? 288
Rdptdmbd ne rsZhmhmf QM? Zmc ehwhmf sn sgd ldlaqZmd 3/3
AZbjfqntmc hm Rntsgdqm Zmc mnqsgdqm gxaqhchyZshnmr Zmc gnv sn Zunhc hs 300
OdqbdmsZfd ne fdk enq otqhexhmf uZqhntr CM? eqZfldmsr 32/
Dwodbsdc lnahkhshdr ne sqZbjhmf cxdr %CM?( 320
Unktldr ne onkxZbqxkZlhcd qdpthqdc sn bZrs lhmhfdkr ne uZqhntr odqbdmsZfdr 321
Oqnlnsdq rdptdmbdr qdbnfmhydc ax aZbsdqhnogZfd,dmbncdc QM? onkxldqZrdr 327
BG:OSDQ 6
OnkxldqYrd BgYhm QdYbshnm
Oqhldq cdrhfm 346
Cdsdbshmf) ZmZkxyhmf) Zmc ptZmshexhmf lQM?r 354
DmgZmbdqr bnllnmkx trdc sn hloqnud sgd deehbhdmbx ne ZlokhehbZshnm
ne FB,qhbg sdlokZsdr 374
SgdqlnrsZakd CM? onkxldqZrdr9 Oqnodqshdr 4/7
SgdqlnrsZakd CM? onkxldqZrdr9 ?ookhbZshnmr 4/7
DwZlokdr ne bnlldqbhZk jhsr enq nmd,rsdo qdudqrd sqZmrbqhoshnm OBQ 402
Bnmchshnmr enq rsZmcZqc OBQr bZsZkxydc ax SZp CM? onkxldqZrd 423
BG:OSDQ 7
AhnhmenqlYshbr
RtllZqx ne rdudqZk AK?RS uZqhZmsr 444
Khrs ne nsgdq oqhldq cdrhfm snnkr 458
BG:OSDQ 8
PtYmshsYshnm ne CM: Ymc QM: ax QdYk,Shld OnkxldqYrd BgYhm QdYbshnm
RtllZqx ne qdZk,shld OBQ bgdlhrsqhdr 522
Bnllnmkx trdc qdonqsdq Zmc ptdmbgdq cxdr enq qdZk,shld OBQ 525

Khrs ne SZakdr wwuhh
Bnllnmkx trdc qdonqsdq“ptdmbgdq oZhqr enq SZpLZm oqnadr 525

Bnllnmkx trdc qdonqsdq“ptdmbgdq oZhqr enq lnkdbtkZq adZbnm oqnadr 526
Bnllnmkx trdc qdonqsdq“ptdmbgdq oZhqr enq Rbnqohnm oqnadr 527
BG:OSDQ 0.
Mtbkdhb :bhc OkYsenql Sdbgmnknfhdr
S6 khmdZq ZlokhehbZshnm ne CM? %SK?C( ne rlZkk CM? lnkdbtkdr 6/2
Rdbnmc,rsqZmc rxmsgdrhr vhsg khlhshmf oqhldq Zlntmsr 6/6
BG:OSDQ 00
CM: Rdptdmbhmf
BnloZqhrnm ne uZqhntr mdws,fdmdqZshnm rdptdmbhmf okZsenqlr 633
Rdptdmbdr ne ZcZosnqr Zmc oqhldqr 7/8
Unktldr ne chktsdc CM? kZccdq Zmc ne ?LOtqd adZcr enq dZbg
adZcr9CM? qZshn 710
Noshltl qZshnr ne sgd CM? bnmbdmsqZshnm hm sgd knv,lnkdbtkZq,vdhfgs
odZjr sn sgd 8//,ao odZj 711
MDWSekdw AZqbncd bnlahmZshnmr 724
BG:OSDQ 01
:mYkxrhr ne CM: LdsgxkYshnm hm LYllYkhYm Bdkkr
Nudquhdv ne ldsgncr enq CM? ldsgxkZshnm ZmZkxrhr 785
BnlldqbhZk jhsr enq ahrtkehsd bnmudqrhnm ne CM? 8/3
BnlldqbhZk jhsr sn Zhc vhsg LR,OBQ 8/7
BnlldqbhZk jhsr enq hlltmnoqdbhohsZshnm,aZrdc cdsdbshnm
ne CM? ldsgxkZshnm 802
OqnfqZlr enq sgd hcdmshehbZshnm ne BoF hrkZmcr 826
OqnfqZlr enq sgd cdrhfm ne oqhldqr enq ltkshokd fdmnlhb knbh 828
RtllZqx ne cZsZ Zmc rsZshrshbZk ZmZkxrhr ldsgncr trdc ax cheedqdms
fdmnld,vhcd ghfg,sgqntfgots cddo,rdptdmbhmf CM? ldsgxkZshnm rstchdr 83/
BG:OSDQ 02
OqdoYqYshnm ne KYadkdc CM:) QM:) Ymc Nkhfnmtbkdnshcd Oqnadr
Ldsgncr trdc sn kZadk mtbkdhb Zbhcr hm uhsqn 834
Hmnrhmd,bnmsZhmhmf bncnmr qdbnlldmcdc enq trd hm
nkhfnmtbkdnshcd oqnadr 850
Dmc,kZadkhmf ne CM? 0//3
Odqbdms onkxZbqxkZlhcd qdpthqdc sn qdrnkud nkhfnmtbkdnshcdr 0/2/
Sgd cnlZhmr ne D- bnkh CM? onkxldqZrd H 0/33
Oqnlnsdq rdptdmbdr qdbnfmhydc ax aZbsdqhnogZfd,dmbncdc
QM? onkxldqZrdr 0/4/
BG:OSDQ 03
Ldsgncr enq Hm Uhsqn LtsYfdmdrhr
BnloZqhrnm ne ltsZfdmdrhr oqnsnbnkr 0/5/
BnlldqbhZk jhsr ZuZhkZakd enq ltsZfdmdrhr oqnsnbnkr 0/55

wwuhhh Khrs ne SZakdr
Oqhldqr trdc enq sgd bknmhmf ne CM? Onk a) Onk b) Zmc Onk h bCM?r
Zmc enq sgd bnmrsqtbshnm ne qZmcnl ltsZfdmdrhr khaqZqhdr 0/65
TmhudqrZk bncnm bZrrdssd cdrhfm 000/
BG:OSDQ 04
Hmsqnctbhmf Fdmdr hmsn Btkstqdc LYllYkhYm Bdkkr
SqZmredbshnm ldsgncr 0023
BnlldqbhZk jhsr Zmc qdZfdmsr enq sqZmredbshnm 0026
Chldmrhnmr ne chrgdr trdc enq bdkk btkstqd 0030
QdbnlldmcZshnmr enq dkdbsqnonqZshnm trhmf sgd Ahn,QZc RgnbjOnc 0065
Rdkdbshud F307 bnmbdmsqZshnm qZmfdr 008/
Rdkdbshud bnmbdmsqZshnmr ne Gxfqnlxbhm A 0080
Rdkdbshud bnmbdmsqZshnmr ne AkZrshbhchm R 0081
BG:OSDQ 05
Hmsqnctbhmf Fdmdr hmsn LYllYkhYm Bdkkr9 UhqYk Udbsnqr
DwZlokdr ne gdkodq bdkk khmdr sgZs rtoonqs sgd fqnvsg ne Zcdmnuhqtr
D0 ltsZmsr 0104
Qdfhnmr ne sgd uhqZk fdmnld sgZs qdftkZsd CM? rxmsgdrhr Zmc sqZmrbqhoshnm
Zqd bktrsdqdc hm sgd svn KSQr sgZs bnmrhrs ne sgqdd etmbshnmZk qdfhnmr 0107
BnloZqhrnm ne lZinq bgZqZbsdqhrshbr ne entq bnllnmkx trdc QM? Zmc
CM? uhqZk udbsnqr 0112
Bgnhbdr ne aZbjanmdr enq lnkdbtkZq bknmdr ne Zcdmnuhqtr
udbsnqr 0113
SZqfds shrrtd “rodbhehb ??U bZorhc rdkdbshnm 0116
?lhmn Zbhc rdptdmbd ne dohsnod sZfr 0217
BG:OSDQ 06
:mYkxrhr ne Fdmd QdftkYshnm Trhmf Qdonqsdq Rxrsdlr
BnloZqhrnm ne bnllnmkx trdc qdonqsdq fdmdr 0226
Bnllnm dmgZmbdqr trdc hm sgd bgdlhktlhmdrbdms cdsdbshnm ne
a,fZkZbsnrhcZrd Zbshuhsx 024/
Oqnodqshdr ne rdkdbsdc lnmnldqhb ektnqdrbdms oqnsdhmr 0271
Oqnodqshdr ne rdkdbsdc chldqhb Zmc sdsqZlhb ektnqdrbdms oqnsdhmr 0272
Bnllnm dohsnod sZfr 0284
OqnsdZrd bkdZuZfd rhsdr bnllnmkx trdc enq dohsnod sZf qdlnuZk 0286
BG:OSDQ 07
QM: Hmsdqedqdmbd Ymc RlYkk QM: :mYkxrhr
crQM? Zmc rhQM? cdrhfm snnkr 031/
Unktldr ne bdkkr) CgZqlZEDBS 3) Zmc rhQM?r %nq ?RNr( trdc enq
sqZmredbshnm ne btkstqdc lZllZkhZm bdkkr 0323
Unktldr ne bdkkr) CgZqlZEDBS 3) Zmc crQM? %rhQM?r nq ?RNr( trdc enq
sqZmredbshnm ne btkstqdc CqnrnoghkZ bdkkr 0326
Unktldr ne bdkkr) crQM? trdc enq rnZjhmf btkstqdc CqnrnoghkZ bdkkr 0334
Khrs ne gtlZm Zmc lntrd rgQM?.rhQM? khaqZqhdr ZuZhkZakd enq
fdmnld,vhcd QM?h rbqddmr 0362

Khrs ne SZakdr wwhw
BG:OSDQ 08
Dwoqdrrhmf Bknmdc Fdmdr enq Oqnsdhm Oqnctbshnm) OtqhehbYshnm) Ymc :mYkxrhr
BnloZqhrnm ne gnrsr enq qdbnlahmZms oqnsdhm oqnctbshnm 0372
Bnllnm udbsnqr enq oqnsdhm dwoqdrrhnm 0378
Bnllnm etrhnm oqnsdhmr trdc enq qdbnlahmZms oqnsdhm otqhehbZshnm 04/0
Bnllnm dmcnodoshcZrdr trdc enq bkdZuZfd ne qdbnlahmZms etrhnm oqnsdhmr 04/1
Deedbshud qZmfd ne rdoZqZshnm ne RCR “onkxZbqxkZlhcd fdkr 05//
Rnktshnmr enq oqdoZqhmf qdrnkuhmf fdkr enq Sqhr,fkxbhmd RCR,O?FD 05/1
Rnktshnmr enq oqdoZqhmf 4 rsZbjhmf fdkr enq Sqhr,fkxbhmd RCR,O?FD 05/1
Ateedqr enq sqZmredq ne oqnsdhmr eqnl onkxZbqxkZlhcd fdkr sn ldlaqZmdr 0506
Bgqnlnfdmhb Zmc bgdlhktlhmdrbdms ldsgncr ne cdsdbshnm ne
hllnahkhydc Zmshfdmr 051/
OgxrhbZk oqnodqshdr ne cdsdqfdmsr bnllnmkx trdc hm ldlaqZmd
oqnsdhm otqhehbZshnm 0523
BG:OSDQ 1.
Bqnrr,Khmihmf Sdbgmnknfhdr enq :mYkxrhr ne BgqnlYshm Rsqtbstqd Ymc Etmbshnm
Nudquhdv ne sgd BgHO,aZrdc ldsgncr 0530
Nudquhdv ne sgd edZstqdr ne 2B,aZrdc ldsgncr 0535
BG:OSDQ 10
LYoohmf ne Hm Uhun QM:,Ahmchmf Rhsdr ax TU,Bqnrr,Khmihmf HlltmnoqdbhohsYshnm BKHO(
QdoqdrdmsZshud QM?,ahmchmf oqnsdhmr rstchdc ax BKHO 06/4
UZqhZms BKHO oqnsnbnkr 06/8
BG:OSDQ 11
FYsdvYx,BnloYshakd XdYrs Nmd,Gxaqhc Ymc Svn,Gxaqhc :rrYxr
X1G CA,aZhs rdkdbshnm 0654
X1G ?C,oqdx rdkdbshnm 0655
X0G CM?,aZhs rdkdbshnm 0656
X0G ?C,oqdx rdkdbshnm 0657
?cuZmsZfdr Zmc chrZcuZmsZfdr ne X1G Zmc X0G ZrrZxr 0657
RsqZhmr Zmc lZqjdqr trdc hm X0G Zmc X1G ZrrZxr 066/
Khrs ne udbsnqr Zmc qdkZsdc oqhldqr 066/
DuZktZshmf X0G.X1G hmsdqZbshnmr 0686
:OODMCHW 0
QdYfdmsr Ymc Ateedqr Trdc hm LnkdbtkYq Bknmhmf
OqdoZqZshnm ne Sqhr ateedqr ne uZqhntr cdrhqdc oG uZktdr 0717
Oqnodqshdr ne fnnc ateedqr 072/
OqdoZqZshnm ne /-0 L onsZrrhtl ognrogZsd ateedq Zs 14´B 0720
OqdoZqZshnm ne /-0 L rnchtl ognrogZsd ateedq Zs 14´B 0720
BnmbdmsqZshnmr ne Zbhcr Zmc aZrdr9 bnllnm bnlldqbhZk rsqdmfsgr 0722
?ooqnwhlZsd oG uZktdr enq uZqhntr bnmbdmsqZshnmr ne rsnbj rnktshnmr 0722
Bnllnmkx trdc Zmshahnshb rnktshnmr 0724
?mshahnshb lncdr ne Zbshnm 0724

www Khrs ne SZakdr
:OODMCHW 2
Cdsdbshnm Rxrsdlr
Bgdlhktlhmdrbdms ZrrZxr enq hlltmnZrrZx Zmc mtbkdhb Zbhc
gxaqhchyZshnm kZadkr 075/
?ookhbZshnmr ne bgdlhktlhmdrbdmbd hm lnkdbtkZq ahnknfx 0750
Ldsgncr ne kZadkhmf mtbkdhb Zbhcr vhsg chfnwxfdmhm 076/
Ahmchmf ne oqnsdhm ? Zmc oqnsdhm F sn sgd Eb qdfhnm ne
lZllZkhZm hlltmnfknatkhmr 0768
Ahmchmf ne hlltmnfknatkhmr sn oqnsdhm K) oqnsdhm ?) Zmc oqnsdhm F 0770

OqdeYbd
S GD QD;CW ;U;HK;AHKHSW NE SGD BNLOKDSD FDMNLD RDPTDMBDR enq gtlZmr Zmc lncdk nqfZmhrlr
gZr oqnentmckx Zeedbsdc sgd vZx hm vghbg ahnknfhrsr ne dudqx chrbhokhmd mnv oqZbshbd rbhdmbd-
DwoknqZshnm ne sgd uZrs fdmnlhb kZmcrbZod qdpthqdc sgd cdudknoldms ne Z fqdZs uZqhdsx ne mdv dwodq,
hldmsZk sdbgmhptdr Zmc ZooqnZbgdr- HmduhsZakx) udmdqZakd bknmhmf lZmtZkr adbZld ntscZsdc Zmc
drsZakhrgdc ldsgncr vdqd qdmcdqdc narnkdsd- Sgdrd dudmsr oqnuhcdc sgd lZinq hlodstr enq Z sgnq,
ntfg qduhrhnm ne InidbtiUp Ainmhmf-
Hm sgd ehqrs rsZfdr ne InidbtiUp Ainmhmf , LB3() vd tmcdqsnnj Zm dwsdmrhud qduhdv oqnbdrr sn
cdsdqlhmd vgZs nkc lZsdqhZk rgntkc ad jdos) vgZs mdv lZsdqhZk rgntkc ad Zccdc) Zmc) lnrs cheehbtks
ne Zkk) vgZs lZsdqhZk rgntkc ad cdkdsdc- Sgd mZldr ne sgd lZmx rbhdmshrsr vgn bnmsqhatsdc hmuZktZakd
Zcuhbd ctqhmf sgd qduhdv oqnbdrr Zqd khrsdc nm sgd enkknvhmf Zbjmnvkdcfldmsr oZfd- Vd Zqd fqZsdetk
sn sgdl Zkk-
Hs hr ne bntqrd hlonrrhakd sn hmbktcd hm Zmx rhmfkd kZanqZsnqx lZmtZk Zkk dwodqhldmsZk ldsgncr
trdc hm lnkdbtkZq ahnknfx) Zmc rn bgnhbdr) rnldshldr sntfg nmdr) gZc sn ad lZcd- HmchuhctZkr lZx
chrZfqdd vhsg rnld ne ntq bgnhbdr Zmc vd rtrodbs rnld vhkk(- Gnvdudq) ntq svn fthchmf oqhmbhokdr
Zqd Zr enkknvr- Ehqrs) InidbtiUp Ainmhmf hr Z zmtbkdhb Zbhc “ bdmsqhb– kZanqZsnqx lZmtZk) Zmc sgdqdenqd
hm fdmdqZk vd gZud mns hmbktcdc ldsgncr sgZs cn mns chqdbskx hmunkud CM; nq QM;- Rn) Zksgntfg
LB3 bnmsZhmr Z bgZosdq nm xdZrs svn,gxaqhc oqnbdctqdr enq ZmZkxyhmf oqnsdhm “ oqnsdhm hmsdqZbshnmr)
vd chc mns hmbktcd sgd lZmx nsgdq ZooqnZbgdr enq rstcxhmf oqnsdhm “ oqnsdhm hmsdqZbshnmr sgZs cn mns
chqdbskx hmunkud mtbkdhb Zbhcr- Rdbnmc) vd gZud Zssdlosdc sn hmbktcd Zr lZmx Zr onrrhakd ne sgnrd
mtbkdhb Zbhc “ aZrdc ldsgncr sgZs Zqd vhcdkx trdc hm lnkdbtkZq Zmc bdkktkZq ahnknfx kZanqZsnqhdr
vhsg sgd gnod hm sgd Ingm KnbjdZm rdmrd sgZs vd zcn sgd fqdZsdrs fnnc enq sgd lnrs odnokd-– Sgd
gZqcdq sZrj enq tr vZr sn cdbhcd vghbg lZsdqhZk sn cdkdsd- Gnvdudq) ntq sZrj vZr lZcd rnldvgZs
dZrhdq ax sgd Zfqddldms ne ntq otakhrgdq) Bnkc Roqhmf GZqanq KZanqZsnqx Oqdrr) sn lZjd sgdrd nkcdq
ldsgncr eqddkx ZuZhkZakd nm sgd Anic Pophmf GUpanp Opnsnbnir vdarhsd vvv-brgoqnsnbnkr-nqf(-
Sgd dwoknrhnm ne mdv dwodqhldmsZk ZooqnZbgdr gZr lZcd hs hloqZbshbZk) he mns hlonrrhakd) enq
Zmx rhmfkd odqrnm nq dudm svn( sn vqhsd vhsg Ztsgnqhsx nm Zkk sgd qdkduZms dwodqhldmsZk ldsgncr- ;r
Z qdrtks) Z lZinq cdoZqstqd eqnl oqduhntr dchshnmr ne InidbtiUp Ainmhmf vZr sgd bnllhrrhnmhmf ne
dwodqsr hm sgd ehdkc sn vqhsd rodbhehb bgZosdqr Zmc sn bnmsqhatsd rodbhehb oqnsnbnkr- Vhsgnts sgd
dmsgtrhZrshb oZqshbhoZshnm ne sgdrd rbhdmshrsr) LB3 rhlokx vntkc mns dwhrs-
Rhmbd sgd kZrs dchshnm ne InidbtiUp Ainmhmf) sgdqd gZr addm Z qdkdmskdrr Zmc bnmshmthmf oqnkhe,
dqZshnm ne bnlldqbhZk zjhsr)– vghbg hr ansg Z akdrrhmf Zmc Z btqrd- Nm sgd nmd gZmc) jhsr needq sqd,
ldmcntr bnmudmhdmbd) oZqshbtkZqkx enq oqnbdctqdr sgZs Zqd mns qntshmdkx trdc hm Zm hmchuhctZk
kZanqZsnqx- Nm sgd nsgdq gZmc) jhsr bZm nesdm ad snn bnmudmhdms) dmZakhmf trdqr sn odqenql oqnbd,
ctqdr vhsgnts tmcdqrsZmchmf sgd tmcdqkxhmf oqhmbhokdr ne sgd ldsgnc- Vgdqd onrrhakd) vd gZud
Zssdlosdc sn cdZk vhsg sghr chkdllZ ax oqnuhchmf khrsr ne bnlldqbhZkkx ZuZhkZakd jhsr Zmc Zkrn
cdrbqhahmf gnv sgdx vnqj-
Sgdqd Zqd lZmx odnokd vgn okZxdc drrdmshZk qnkdr hm sgd oqnctbshnm ne LB3 Zmc vgnl vd
fqZsdetkkx Zbjmnvkdcfd- ;mm Anxkd vZr hmrsqtldmsZk hm fdsshmf LB3 nee sgd fqntmc Zmc okZxdc Z
bqhshbZk nqfZmhyZshnmZk qnkd ctqhmf sgd dZqkx rsZfdr ne sgd oqnidbs- Rtardptdmskx) gdq qdronmrhahkhshdr
vdqd sZjdm nudq ax sgd Zakd ZrrhrsZmbd ne ;kdw FZmm- RZqZ Cdhakdq bnmsqhatsdc Zs Zkk rsZfdr ne
wwwh

wwwhh OqdeZbd
LB3 hm lZmx vZxr) ats hm oZqshbtkZq hm Zrrhrshmf vhsg vqhshmf) dchshmf) Zmc oqnneqdZchmf- LnmhbZ
RZkZmh lZcd rtarsZmshZk bnmsqhatshnmr sn sgd bnmsdms Zmc vqhshmf ne BgZosdq 8-
Vd Zqd drodbhZkkx fqZsdetk enq sgd dmsgtrhZrshb rtoonqs) dwsqZnqchmZqx bnnodqZshnm) Zmc snkdq,
Zmbd ne sgd rsZee ne sgd Bnkc Roqhmf GZqanq KZanqZsnqx Oqdrr˚hm oZqshbtkZq IZm ;qfdmshmd) vgn
lZmZfdc sgd dmshqd oqnidbs Zmc jdos Z bknrd dxd nm hsr ehmZmbdr: LZqxkhy Chbjdqrnm) ntq oqnidbs lZm,
Zfdq: ntq cdudknoldmsZk dchsnqr JZZqdm IZmrrdm) Itcx Btcchgx) Zmc LhbgZdk Yhdqkdq: Cdmhrd Vdhrr)
sgd Oqnctbshnm LZmZfdq: ntq oqnctbshnm dchsnq) JZsgkddm Ataadn: Zmc) ne bntqrd) Ingm Hmfkhr) sgd
d”lhmdmbd fqhrd ne Bnkc Roqhmf GZqanq KZanqZsnqx Oqdrr-
LHBG;DK Q- FQDDM
INRDOG R;LAQNNJ
4 Mnsd eqnl sgd Otakhrgdq

QdZcdqr Zqd dmbntqZfdc sn uhrhs sgd vdarhsd vvv-lnkdbtkZqbknmhmf-nqf sn nasZhm to,sn,cZsd hmenq,
lZshnm Zants Zkk Zrodbsr ne sghr annj Zmc hsr bnmsdmsr-

4bjmnvkdcfldmsr
Sgd Ztsgnqr vhrg sn sgZmj sgd enkknvhmf bnkkdZftdr enq sgdhq uZktZakd ZrrhrsZmbd9
G- Dertm ;qcZ MZsgZm KZvrnm MZqdmcqZ VZiZodxdd

LhbgZdk E- BZqdx BgdmfihZm Kh LZqhZm VZkgnts
CZqqxk Bnmsd Khmf Khm Oghkkho YZlnqd
Ina Cdjjdq CnmZkc Qhn LZqhZ YZoo
BkZtcd FZyhm RZqZg RgdooZqc
OZtk JZtelZm Rsdogdm RlZkd
Sgd Otakhrgdq vhrgdr sn sgZmj sgd enkknvhmf9
OZtkZ AtatkxZ Mhbnkd Mhbgnkr AZqsnm RkZsjn

Snl AtatkxZ RZsgddr QZfgZuZm
wwwhhh

FdmdqYk RYedsx Ymc GYyYqcntr LYsdqhYk HmenqlYshnm

Sghr lYmtYk rgntkc ad trdc ax kYanqYsnqx odqrnmmdk vhsg dwodqhdmbd hm kYanqYsnqx Ymc bgdlhbYk
rYedsx nq rstcdmsr tmcdq sgd rtodquhrhnm ne rthsYakx sqYhmdc odqrnmmdk- Sgd oqnbdctqdr’ bgdlhbYkr’
Ymc dptholdms qdedqdmbdc hm sghr lYmtYk Yqd gYyYqcntr Ymc bYm bYtrd rdqhntr hmitqx tmkdrr odq,
enqldc’ gYmckdc’ Ymc trdc vhsg bYqd Ymc hm Y lYmmdq bnmrhrsdms vhsg rYed kYanqYsnqx oqYbshbdr-
Rstcdmsr Ymc qdrdYqbgdqr trhmf sgd oqnbdctqdr hm sghr lYmtYk cn rn Ys sgdhq nvm qhrj- Hs hr drrdmshYk
enq xntq rYedsx sgYs xnt bnmrtks sgd YooqnoqhYsd LYsdqhYk RYedsx CYsY Rgddsr’ sgd lYmteYbstqdqr
lYmtYkr YbbnloYmxhmf dptholdms’ Ymc xntq hmrshstshnm r DmuhqnmldmsYk GdYksg Ymc RYedsx
Neehbd’ Yr vdkk Yr sgd EdmdqYk RYedsx Ymc ChronrYk HmenqlYshnm hm 4oodmchw 3 enq oqnodq gYmckhmf
ne gYyYqcntr lYsdqhYkr cdrbqhadc hm sghr lYmtYk- Bnkc Roqhmf GYqanq IYanqYsnqx lYjdr mn qdoqd,
rdmsYshnmr nq vYqqYmshdr vhsg qdrodbs sn sgd lYsdqhYk rds enqsg hm sghr lYmtYk Ymc gYr mn khYahkhsx hm
bnmmdbshnm vhsg sgd trd ne sgdrd lYsdqhYkr-
4kk qdfhrsdqdc sqYcdlYqjr’ sqYcd mYldr’ Ymc aqYmc mYldr ldmshnmdc hm sghr annj Yqd sgd oqno,
dqsx ne sgd qdrodbshud nvmdqr- PdYcdqr rgntkc bnmrtks hmchuhctYk lYmteYbstqdqr Ymc nsgdq qdrntq,
bdr enq btqqdms Ymc rodbhehb oqnctbs hmenqlYshnm- 4ooqnoqhYsd rntqbdr enq nasYhmhmf rYedsx
hmenqlYshnm Ymc fdmdqYk fthcdkhmdr enq kYanqYsnqx rYedsx Yqd oqnuhcdc hm sgd EdmdqYk RYedsx Ymc
GYyYqcntr LYsdqhYk Yoodmchw ne sghr lYmtYk-

Molecular Cloning

Uploaded by

Document Information

Original Description:

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Molecular Cloning

Uploaded by

Copyright:

Available Formats

This is a free sample of content from Molecular Cloning: A Laboratory Manual, 4th edition.

Click here for more information or to buy the book.

© 2012 by Cold Spring Harbor Laboratory Press

NSGDQ SHSKDR EQNL BRGK OQDRR

© 2012 by Cold Spring Harbor Laboratory Press

BNIC ROPHME G4PANP I4ANP4SNPT OPDRR

© 2012 by Cold Spring Harbor Laboratory Press

Mtakhrgdq Ingm Hmfkhr

Anudq Sqs chqdbshnm Smc cdrhfm9 Odsd Ideer) 1/01

KhaqZqx ne Bnmfqdrr BZsZknfhmf,hm,OtakhbZshnm CZsZ

Fqddm) LhbgZdk Q- LhbgZdk QhbgZqc() 0843,

© 2012 by Cold Spring Harbor Laboratory Press

Khrs ne SZakdr wwu

© 2012 by Cold Spring Harbor Laboratory Press

© 2012 by Cold Spring Harbor Laboratory Press

02 Rntsgdqm GxaqhchyZshnm ne QZchnkZadkdc Oqnadr sn Mtbkdhb ?bhcr

© 2012 by Cold Spring Harbor Laboratory Press

Bnmcdmrhmf Zmc Bqnvchmf QdZfdmsr 13/

© 2012 by Cold Spring Harbor Laboratory Press

7 DkdbsqnonqZshnm ne Bnlodsdms A?B Gnrs Bdkkr vhsg sgd QdbnlahmZms

© 2012 by Cold Spring Harbor Laboratory Press

02 SqZmredq Zmc EhwZshnm ne CdmZstqdc QM? hm OnkxZbqxkZlhcd Fdkr sn LdlaqZmdr ax

© 2012 by Cold Spring Harbor Laboratory Press

© 2012 by Cold Spring Harbor Laboratory Press

© 2012 by Cold Spring Harbor Laboratory Press

RDBSHNM H- KHAQ:QX OQDO:Q:SHNMR ENQ U:QHNTR OK:SENQLR

RDBSHNM HH- ONRSRDPTDMBHMF :M:KXRDR

© 2012 by Cold Spring Harbor Laboratory Press

© 2012 by Cold Spring Harbor Laboratory Press

00 OgnrognqxkZshnm ne CM? Lnkdbtkdr vhsg CdognrognqxkZsdc Aktms Dmcr nq

© 2012 by Cold Spring Harbor Laboratory Press

O:QS 29 HMSQNCTBHMF FDMDR HMSN BDKKR

© 2012 by Cold Spring Harbor Laboratory Press

KhmdZqhyhmf OkZrlhcr adenqd SqZmredbshnm 0086

© 2012 by Cold Spring Harbor Laboratory Press

06 ShsqZshnm ne Kdmshuhqtr Udbsnqr 0203

© 2012 by Cold Spring Harbor Laboratory Press

© 2012 by Cold Spring Harbor Laboratory Press

1 Dwoqdrrhnm ne Bknmdc Fdmdr Trhmf sgd AZbtknuhqtr Dwoqdrrhnm Rxrsdl 0416

O:QS 49 HMSDQ:BSHNM :M:KXRHR

© 2012 by Cold Spring Harbor Laboratory Press

© 2012 by Cold Spring Harbor Laboratory Press

:BHCR :MC A:RDR 0722

NQF:MHB QD:FDMSR 0723

© 2012 by Cold Spring Harbor Laboratory Press

AKNBIHMF :FDMSR 0725

A:BSDQH:K RSNQ:FD LDCH: 0728

ODQHNCHB S:AKD 073/

QD:FDMSR :MC ATEEDQR HMCDW 0730

OQDO:Q:SHNM NE FK:RRV:QD :MC OK:RSHBV:QD 0732

OQDO:Q:SHNM NE CH:KXRHR STAHMF 0734

DRSHL:SHNM NE BDKK MTLADQ 0735

LD:RTQDLDMS NE Q:CHN:BSHUHSX HM MTBKDHB :BHCR 0738

CDBNMS:LHM:SHNM NE RNKTSHNMR BNMS:HMHMF DSGHCHTL AQNLHCD 0740

RS:HMHMF MTBKDHB :BHCR 0744

© 2012 by Cold Spring Harbor Laboratory Press

© 2012 by Cold Spring Harbor Laboratory Press

© 2012 by Cold Spring Harbor Laboratory Press

wwuh Khrs ne SZakdr

© 2012 by Cold Spring Harbor Laboratory Press

Khrs ne SZakdr wwuhh

Bnllnmkx trdc qdonqsdq“ptdmbgdq oZhqr enq SZpLZm oqnadr 525

© 2012 by Cold Spring Harbor Laboratory Press