39th Hybrid Annual Meeting of the ESHRE, Copenhagen – Denmark, 25-28 June 2023 i561

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.. Abstract citation ID: dead093.1104
.. P-798 Comprehensive evaluation of autologous Platelet-Rich
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.. Plasma infusion in ovarian rejuvenation
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.. A. Garavellas1, P. Mallis2, E. Michalopoulos3, E. Nikitos4
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.. 1
Institute of Life- IASO Maternity Hospital, iolife ivf, Athens, Greece
.. 2
Biomedical Research Foundation Academy of Athens, Biomedical Research
.. Foundation Academy of Athens, athens, Greece
.. 3
.. Hellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens,
.. athens, Greece
.. 4
Institute of Life-, IASO Maternity Hospital, athens, Greece
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.. Study question: This study aimed to the evaluation of PRP injection in
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.. women with ovarian dysfunction (with POR, POI, perimenopause and meno-
.. pause) to promote the ovarian rejuvenation.
.. Summary answer: Intraovarian infusion of autologous PRP exhibited re-
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.. markable evidence and promising results to restore ovarian insufficiency.
.. What is known already: Ovarian dysfunctions, such as premature ovarian
.. insufficiency (POI) and poor ovarian response (POR) are conditions, charac-
.. terized by the collapse of ovarian function and are considered currently as a
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.. global public health issue. Primary factors that are associated with female in-
.. fertility include endocrine dysfunction, failure of embryo implantation, endo-
.. metriosis, and other related pathologies such as polycystic ovary syndrome
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.. (PCOS), various environmental factors and inflammatory disease. For this pur-
.. pose, the rejuvenation of the ovaries using the intraovarian PRP injections
.. may result in increased release of follicles from the available reservoir, and
.. can potentially increase the possibility of a successful pregnancy.
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.. Study design, size, duration: This study was designed as a randomized
.. prospective observational pilot study. A number of 582 participants were in-
.. terested to participate. The whole study was performed between May 2018
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.. and December 2021. The patients were subdivided into 5 groups, followeeed
.. by autologous blood collection, PRP preparation and intraovarian injection.
.. The follow-up of the patients lasted for 2 months.
.. Participants/materials, setting, methods: The classification of the partici-
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.. pants was performed into the following groups: Group A (22-38 years),
.. Group B (39-44 years), Group C (45-57 years), Group D (48-50 years) and
.. Group E (51-56 years). 60 ml of autologous blood was collected from each
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.. patient, followed by the preparation of PRP. Intraovarian PRP injection was
.. performed and monitoring of the ovarian function including the quantification
.. of FSH, LH, AMH and E2 for two constitutive menstrual cycles, was
.. performed.
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.. Main results and the role of chance: For group A, decrease of more than
.. 50% was detected for the FSH and LH whereas, the levels of E2 and AMH
.. were elevated by more than 44% and 31%, respectively in all participants
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.. Moreover, statistically significant differences regarding the levels of FSH
.. (p ¼ 0.001), LH (p ¼ 0.046) , E2 (p ¼ 0.035) and AMH (p ¼ 0.012), were ob-
.. served after the 1st and 2nd month of follow-up. In group B, a statistically sig-
.. nificant decrease in FSH (p < 0.001) and LH (p ¼ 0.001) levels were observed
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.. in all participants. In parallel, the elevation of E2 and AMH levels was ob-
.. served. Moreover, 28% of the participants with diagnosed POI, after the PRP
.. infusion, achieved successful pregnancies and live births. In group C, after the
.. PRP infusion, a reduction in FSH and LH levels with a parallel increase of E2
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.. and AMH levels was detected. In group D, the levels of FSH and LH slightly
.. declined after the PRP infusion. Also, the levels of E2 and AMH were in-
.. creased. In group E, the levels of FSH and LH declin
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.. Limitations, reasons for caution: No classifications between responders
.. and no responders after the PRP administration were performed.
.. Additionally, no presence of control groups in each category nor the determi-
.. nation of the growth factor content of the produced PRP was performed.
i562 39th Hybrid Annual Meeting of the ESHRE, Copenhagen – Denmark, 25-28 June 2023

The relatively limited time for the follow-up can also be considered a further ..
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limitation. ..
Wider implications of the findings: The administration of autologous PRP ..
can be considered a safe and tolerable alternative approach to the classical ..
ones for ovarian rejuvenation. This in turn could result to better conceiving
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rates, thus may beneficially improve the social impact of women with ovarian ..
dysfunction. ..
Trial registration number: 10/10/19
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