Dated

29
ge No.

LxPERIMENT No. 7
detesmine the disti bu| ion Cottut
Aim : Jo Benzoic acid
Patition Colyiient
water at ooYn dempasatue .
benzene ond
bottle GBumete , fipet
Jitration

Josmula : K = JCorg
Caq,
Che micals: Benzoic Acid, Benzene , N1o NaoH, N
phonol phthalein inelícatoy
NaoH,
Observations
oith No0 NaoH 23
A) 4queos daycr
Aqueous daycr with hoo NaDH
Volume o Soluhon tdken o Jih atIon 5rnl
9ottial Jinas Volume
Botle No. Buretle NaoH SoiHured
Buyee ReodAccbing (m)
6-5 6-5mL
6-5 13 6 5ml
13 145 G5mk
Ve Concoco aui Reading = 65myate
30 lo ,Do 3
Dated ............,./.............

kxeERIMENT No.7
the dístibutíon Coelliient
lartitan Caciciert ok Benzoic aid
beteern benzene ondl koter at 30orm. dumetal
botle Buxete.
TRtraton sk measung cylindor eta
fostztion Canjioient (K) =Co
Concantation a Salute cQ
-ant ond "lader tsprativey
Chemic als: Benzoio Atd , Bomene , Nlo NaoH
N| l00 Na0H bhenolah thatin indiato.
ioce duse : ) Rasbote. sthe olouing. Solutions:
Botle No. Volime of| Volume ohVolume of Jotal
Benzoic Aid Benzene(n) Water(i) Volume
m
1 4o ml Jo m 50 mL l00ml

2 30 mL 20 m 50 m l00 m
 Dated .....
Page No. *****..

49-5
255 3:5 60 mL
34-5 34-5 60m
concord ontRtaiking

B) For Oxganic dayer with Nho NaoH :

Benzene dayer oHh NIo Na 0o
Sor^ aken Joy each titation = Brml.
Yolu me o
Botle No. Sntitial 3inal Busetle Volum eo Nasi
Busete Reoding Rending. Sof? Usedm
1-5 45mb
45 75mb
15 225 75mb
Qoncordont Redling(Yi 5ml
Aa-5 29-5 001

14
Cancordo nt Reodling (y)eTm
Galculations: Room dem porature: 210
A) Calaulatin o Maloy Concentvotion o BenzoTo Aid. in aqueas
Nemality MY 6:5 x - 65 N.
l00 5 500
stensth = 65 122 = 59 g12l
Stgtatore
 32
PageNo. ted.0, 203
Dated

2) Shake Both the bott les

3) Allow botles to stond undistudbed tos 16 min.
l| hatexand benzene. Seborate as too.
Jayas Jhe isthat BenzAne
Jhe Lowex eyuater.
Jitration aqubus tayex with NDo Na.oH :
Pripetteot 5ml. iautauaayer om Boitl
to Gtation osk
Jitate it against N100 Soltion o Sodium
hydiokide The end end boiat is "the appcoYance
Pok Coloux Repeatthe htotion to
thxee
tote the aqueous Layez Bottlel
No. 2.

9) Khration o Crganic daycx with Njo NaoH:

Pipete out 5a. Benzene dayez
bottle ond ttxate tt against N|1o
Na.OH Solution Jalen in buxetteHge Phenol
- þhthalene as 9ndicatae
Yetullond. Nste
6). 0bseovethe
don them
Reáatags"
 19
Page No. 33 Dated
|o202?
I...

.******.
= Oo13 molesL
MGHso= 59
122

x l =6
Botle 2) Nogmaiypo
(N,)
=6
5 500

4500 x122 464 g1

stvengh =
CH,o = -464 = 0 O12 moley
(2 2

6) Calulation Molax Cnentratier

Molas. Cncentra tiern aenawt 4ud
Bottlei)) Noymaliy (M) = 15mt x
x 5
6 N
lo 50

srength=
122

Botlle 2)
gBotlle N 50
N
50
CReraote4d. =.:11-03
"Colualation Pautitiom Coel|iaent
Botile NoBenoic Acid k- Bera. pidlayo| KeJCsn.Acd (Co
-15 0-o1353 Ko345o-o1g 3i
2 O-14 0"o12 K= o5/o-o12 =3-2

Result KKKz
44 is found that KS Comstont Hence Genzoic gcid eseist
diner ie (Cehs CoOH) 2 Signature
Page
No. 34
Dated 19J0L 203

GENERALCabeulation9:
A Caladaion oj Molor
io 4gueous er Concertation Benzaia ad
Sml of N, "Benzoo Acid - V,Vml o Nioo Na oH
5

Nesmality xBuivalent Deight

litre
1500
Molaz oncenratin
mdes|
8) Calcuation of Molag Concentyotion of Berzoic 4üd
4ud

Notnaliy En:N,3 50

stangth 122= 4
50
9litre

Molag Concentration
Benzot. 4ud
ll, Gacnwlo. Acid and Cu.o aie Caleulate d ox batles
no. 1,2.

K 8)-2,
(Kegult + k, 3!
k B Corstant. Hence. Benibte Hudl
ests as dimex i-c
Page No. 36 Dated I....../ ***...

ExPERIMENTNO.:8
Aim: do sepuate a mitwe of arnino ald

ascbnaing ohoratey
Appaxatus:and.ailany Jub
eto.

(91stonc avdlea)
brSplvent
tonce boscicd)
Soluto (Armings

2om

Signature
pge
No...o6

Dated o | , 203

kxpeREMENTNo. 8
Aim: o sehazote mistuxeo amino
acid
AsCandinghazr chmomatagxabny
Ppaxatus
þakex and multishet. frame.
Psoce
L) Maxk
duxe 6

the stozting ine On the stoip.

about the Ñth help
2.emom One end with

2) Put a coop o mixture o amino A cid.

ithine help
, olloo it
Caplany
to
Jube in Centxe
and odd mose drop
o mixtute to

3) Suspend hete bapet th the Cylinder

kecpin
side inn the
end o Hhe sbot on
on the
dhe Loer
Solvent,alou it to stand and
Can not disturbt tos 3o minutes . until the
Solverst 8os sisen. do the dop
Page No. 34 Dated

Obsex vations:
Bistonce travelled by Solute or
Component (Amwino
6-5 Gm
Aia)
4uid) rom Vosiginal ine
listance trovellec by the Solvent doon the
line = 10 &m

Calcutations :
Distance tiavelled by Solute 0m ird
istone travellecd Solvent thom origial
lire

65 0.65

= 0-65

ResuT: given Jhe Ry (Retention

amiho
Retertion
auid
fadtor)
is 0- 65
valus
be
No.
38
Dated .2..1.!...2023

) Jake out theiltex det it be day

boper
Moxk the Qevel ot Solent oith
Pencil
5) Shray the sapes stoip unitosrnly oith Jhe
rinhdsin Solution and clay it and
ho measuze
c the
heiaht shot tom he din&.

6) Calculate the R4value

to
Rf value s equal
Kh = istance tavelled by Golute hon original Line
Jistance tzovelled by Sol vent om
Dine
Obsez vations :
Bistance. tiayelled by olute or Component
CAmino Aetd) rom 'oríginal line =
Aistonce Jtavelled the Solvent trom oxíginal
dine B Cm.

Jhe Ry CReteation fatar) valtue o

amino acidis0 65
gien