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Textbook of
Clinical Embryology
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Textbook of
Clinical Embryology
Second Edition
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RELX India Pvt. Ltd.
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ISBN: 978-81-312-4882-9
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Dedicated to
My Parents
Late Smt Ganga Devi Singh
and
Late Shri HR Singh an ever guiding force in my life
My Wife
Late Smt Manorama Rani Singh for her unending support and
cooperation of my pursuits
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Preface to the Second Edition
It gives me great pleasure to present second edition of Textbook of Clinical Embryology, which is widely used not only by the
undergraduate students but also by the postgraduate students of anatomy, pediatrics, and obstetrics and gynecology. The
popularity of this book reflects the appeal of its concept building approach and easy to understand language. This approach
has also been retained in this edition with unique problem-solving approach and its utility in highlighting the embryo-
logical basis of clinical problems. Based on a large number of suggestions, criticisms and comments received from the
VS-Chapter-06.indd 71 6/21/2012 3:50:09 PM
students and fellow academicians, the text has been extensively revised for further improvement of the book.
In this edition new features such as learning objectives, facts to remember, new line diagrams, tables, and flowcharts have
been included to further enhance the utility of this book. In addition, at the end of each chapter the summary of timing of
events is given for easy recall. Most of the diagrams are completely revised and redrawn for easy understanding and repro-
ducibility in the exam by the students. The topics on fertilization, gametogenesis, extraembryonic membranes, heart and
blood vessels, urogenital system and sensory organs have been thoroughly revised in detail due to high incidence of con-
genital problems associated with these systems.
In addition, complimentary access to online animations, chapter-wise image bank along with complete e-book is also pro-
vided.
I sincerely hope that the readers will find this edition more interesting and useful than the previous one. I would love to
get fair comments, good or bad, both from students and teachers.
“Providing good teaching material or its source and inspiring the students for learning is the real contribution of a teacher.”
Vishram Singh
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Preface to the First Edition
Textbook of Clinical Embryology has been carefully planned for the first year medical and dental students. It follows the revised
anatomy curriculum of the Medical Council of India. Following the current trends of clinically oriented study of Anatomy,
I have adopted a parallel approach of imparting basic embryological knowledge to students and simultaneously providing
them its applied aspects.
To help students score high in examinations the text is written in simple language. It is arranged in easily understandable
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small sections. While embryological details of little clinical relevance, phylogenetic discussions, and comparative analogies
have been either omitted or described in brief, all clinically important topics are described in detail. Because of increasingly
significant role of molecular biology and genetics in embryology and study of birth defects, the basic molecular and genetic
principles are discussed throughout the text. In addition, a separate chapter on medical genetics has been added. The tables
and flowcharts given in the book summarize important and complex information into digestible knowledge capsules.
Multiple choice questions have been given chapter-by-chapter at the end of the book to test the level of understanding and
memory recall of the students. The numerous simple four-color illustrations and clinical photographs further assist in fast
comprehension and retention of complicated information. All the illustrations are drawn by the author himself to ensure accuracy.
Throughout the preparation of this book one thing I have kept in mind is that thorough knowledge of embryology is
required by Clinicians, especially Gynecologists, Pediatricians, and Pediatric Surgeons for physical examination, prenatal
diagnostic tests, and surgical procedures. Therefore, embryological events relevant to prenatal diagnostic and surgical
procedures are clinically correlated throughout the text. Further, patient-oriented problems and their embryological and
genetic basis are presented at the end of each chapter for problem-based learning so that the students could use their
embryological knowledge in clinical situations. Moreover, keeping in mind the relevance of embryological knowledge
in day-to-day clinical practice, a separate chapter on developmental events during the entire period of gestation and their
application in clinical practice is given at the end of the book.
I pay my heartfelt tribute to all the authors of various embryology books, especially Developing Human: Clinically Oriented
Embryology, 8th edition by Keith L Moore and TVN Persaud, which I have consulted during the preparation of this book.
From Developing Human and few other books, some photographs have been used in this book after obtaining due permission
from concerned authorities (please refer to page 331 for Figure Credits).
As a teacher, I have tried my best to make the book easy to understand and interesting to read. For further improvement
of this book, I would greatly welcome comments and suggestions from the readers. All these comments and suggestions
can be e-mailed at indiacontact@elsevier.com and drvishramsingh@gmail.com.
‘Mind perceives new ideas best only when put to test.’
Vishram Singh
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Acknowledgments
At the outset, I express my gratitude to Dr P Mahalingam, CMD and Hon. Chancellor, Shri VP Gupta, Registrar,
and Dr Yogesh Tripathi, Hon. Vice Chancellor, Santosh University and Dr PS Dhoot, Dean, Santosh Medical College,
Ghaziabad, NCR, Delhi for providing an appropriate academic atmosphere in the university campus and encouragement
which helped me in preparing 2nd Edition of this book.
I sincerely thank all my colleagues in the Department for their assistance.
VS-Chapter-06.indd 71 6/21/2012 3:50:09 PM
I am really indebted to Dr Deepa Singh, Associate Professor Anatomy, Himalayan Institute of Medical Sciences (HIMS),
Dehradun, Uttarakhand; Dr Preeti Srivastava, Associate Professor, NDMC Medical College and Hindu Rao Hospital,
Delhi; and D Krishna Chaitanya Reddy, PhD Scholar, Department of Anatomy, Santosh University for reviewing the final
proofs sincerely.
I gratefully acknowledge the feedback and support received from fellow colleagues in Anatomy of various medical institu-
tions in India and abroad as well, particularly,
• Professors PK Sharma (Head of the Department) Era’s Lucknow and Punita Manik, King George’s Medical College, Lucknow.
• Professors NC Goel and AK Srivastava (Heads of the Department), Hind Institute of Medical Sciences, Barabanki,
Lucknow and Sitapur, UP, respectively.
• Professor Amit Kumar Saxena (Head of the Department), SGT Medical College, Budhera, Gurgaon, Haryana.
• Professor Poonam Kharb, SMS&R, Greater Noida, UP.
• Professor TC Singel (Head of the Department), BJ Medical College, Udaipur, Rajasthan.
• Professor TS Roy (Head of the Department) and Dr Ritu Sehgal, AIIMS, New Delhi.
• Professors RK Suri (Director Professor), and Hitendra Loh, Vardhman Mahavir Medical College and Safdarjang Hospital,
New Delhi.
• Professor Veena Bharihoke (Head of the Department), Rama Medical College, Hapur, Ghaziabad.
• Professor SL Jethani (Dean and Head of the Department), and Dr Aksh Dubey, Himalayan Institute of Medical Sciences,
Jolly Grant, Dehradun.
• Professor SK Jain (Head of the Department), Teerthanker Mahaveer Medical College & Research Centre, Moradabad, UP.
• Professor SD Joshi (Dean and Head of the Department), Sri Aurobindo Institute of Medical Sciences, Indore, MP.
• Professors Renu Mishra (Head of Department), and Vinay Kumar, Saraswathi Institute of Medical Sciences, Hapur, UP.
I eulogize the patience of my daughter, Dr Rashi Singh, son, Dr Gaurav Singh and daughter-in-law, Anupama Singh, for
helping me in the preparation of this manuscript.
Lastly, I gratefully acknowledge the help and cooperation received from my publisher, RELX India Pvt. Ltd., especially
Shabina Nasim (Sr Project Manager–Education Solutions), Renu Rawat (Manager–Content Strategy), Arvind Koul
(Content Strategist), and Goldy Bhatnagar (Sr Content Development Specialist).
Vishram Singh
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List of Animations
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Contents
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xiv Contents
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Introduction and History
of Embryology
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2 Textbook of Clinical Embryology
week of pregnancy to the end of 6th day after birth. Systemic Embryology
It deals with the functional maturation of various or-
Childhood gans and systems that are formed during the embryonic
period.
The period of childhood extends from beginning of the
2nd year to the age of 12 years. The care of children Descriptive Embryology
during this period is exciting because of the constancy It deals with the structure of different organs at various
of change in their growth and development. Children stages of development.
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Introduction and History of Embryology 3
Comparative Embryology
It deals with the study of embryos in various species of
animals.
Experimental Embryology
It deals with the results obtained from experiments of
living embryos/fetuses of the lower animals.
Chemical Embryology
It deals with the biochemical aspect of the prenatal
Fig. 1.1 Dolly, the first cloned sheep.
development.
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4 Textbook of Clinical Embryology
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Introduction and History of Embryology 5
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6 Textbook of Clinical Embryology
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Introduction and History of Embryology 7
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8 Textbook of Clinical Embryology
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Introduction and History of Embryology 9
CLINICAL PROBLEMS
1. The zygote is a diploid single cell formed after fertilization by the union of haploid male and female gametes.
It is also called a single cell embryo.
The term conceptus refers to the product of conception, i.e., embryo and associated extraembryonic membranes.
2. The anatomists divide prenatal development into 3 periods, viz. preembryonic, embryonic and fetal, while clinicians
divide it into 2 periods, viz. embryonic (from fertilization to 8th week) and fetal (from 9th week to birth).
3. Teratology is the branch of embryology that deals with the congenital anomalies and defects.
4. The stem cells are undifferentiated cells, which are capable of multiplication and differentiation into all types of
specialized cells in the body. The cells of embryoblast are capable of generating all the three germ layers: ectoderm,
mesoderm, and endoderm. Hence, cells of embryoblast (inner cell mass) are termed embryonic stem cells. These
can be kept in an undifferentiated state in culture medium. By using growth factors, these can be made to form dif-
ferent tissue cells, e.g., muscle cells, neurons, and blood cells. The diseases that are likely to be benefited by stem cells
are Parkinson’s disease, Alzheimer’s disease, spinal cord injuries, sickle cell anemia, etc.
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Reproductive System
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Reproductive System 11
Urinary bladder
Urethra
Vas deferens
Duct of
epididymis Vasa efferentia (efferent
ductules of testis)
Scrotum
Testis
Lobules of epididymis
Fibrous septa
Efferent ductules
(15–20 in number)
Convoluted seminiferous Duct of epididymis
tubules (2–3 in each lobule)
Vas deferens
Lobules of
testis (200–300)
Fig. 2.2 Schematic vertical section of the testis showing the basic structure of testis, epididymis, and vas deferens.
in propelling the semen. The vas deferens is cord like Prostate Gland
when grasped between thumb and index finger because
of its thick wall and small lumen. Prostate gland is a pyramidal fibromuscular gland of
about the size of a chestnut. It is gray to reddish in
Seminal Vesicles and Ejaculatory Ducts color, and consists mainly of glandular and muscular
tissue.
The seminal vesicle (5 cm long) is a sacculated coiled The prostate gland surrounds the proximal part of
tube adjacent to ampulla of each vas deferens. The paired the urethra and two ejaculatory ducts. It is enclosed by
seminal vesicles secrete a major portion of volume of a thin but strong fibrous capsule. The capsule is con-
ejaculate. These are located behind the bladder near the tinuous with several fibromuscular partitions. The
prostate gland. Each vesicle ends in a small duct that prostatic glands secrete prostatic fluid, which is
joins ampulla of vas deferens to form an ejaculatory poured into the prostatic urethra through 10–20 ducts.
duct. The two ejaculatory ducts are slender tubes that The prostatic fluid contains acid phosphatase, fibrino-
open into the prostatic part of the urethra. The secretion lysin, citric acid, amylase, prostate specific antigen, and
of seminal vesicles is thick and mucous like. It contains prostaglandins. The prostatic fluid forms the bulk of
fructose that provides nutrition to sperms. the semen (i.e., ejaculate).
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12 Textbook of Clinical Embryology
Intramural Ampulla
part Infundibulum
Isthmus
Fimbria of
Perimetrium uterine tube
Myometrium Ovary
Endometrium Round ligament
of ovary
Uterine cavity
Vagina
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Reproductive System 13
below and behind the uterine tubes. Each ovary is at- the increased level of estrogen hormone inhibits the
tached to the upper part of the uterus by the round secretion of FSH from the anterior pituitary. The pitu-
ligament of the ovary. One end of each ovary is in itary gland also secretes luteinizing hormone (LH).
contact with the fimbria of the uterine tube. Under the influence of a large amount of LH, the
The ovary consists of a thick cortex surrounding a very Graafian follicle bursts and ovulation takes place. The
vascular medulla. The cortex surrounding the medulla ovum is released due to action of proteolytic enzymes
consists of a framework of connective tissue covered by formed by the theca externa cells that cause dissolution
the germinal epithelium. Before puberty, it contains of capsular wall. There is plasma transudation within
numerous primordial follicles. After puberty, it con- the follicles. As a result, follicles swell and pressure
tains ovarian follicles in various stages of maturity. Each within them increases. Because of increased intrafol-
follicle contains an ovum. Till puberty the ovaries remain licular pressure and simultaneous dissolution of follicu-
inactive but stroma still contains immature follicles. lar capsular wall, follicles rupture and ovum is released
During childbearing age, one ovarian follicle ma- (ovulation). After ovulation, the empty follicle devel-
tures and ruptures to release its ovum into the perito- ops into corpus luteum, which secretes hormone
neal cavity. This process is called ovulation and recurs progesterone. The corpus luteum degenerates after
(ovarian cycle) throughout the reproductive life of a 10 days if the ovum is not fertilized. The level of pro-
female. If a woman becomes pregnant, the ovarian cycle gesterone decreases, and again the pituitary gland se-
stops temporarily. cretes FSH and a new cycle starts. Thus, the cyclic
changes in the ovary comprising of development
Ovarian Cycle (Figs 2.4 and 2.5) of ovarian follicles, ovulation, and formation of
The ovarian cycle is the cyclic release of ovum from corpus luteum constitute the ovarian cycle.
the ovary. This cycle is controlled by hormones secreted The corpus luteum persists for 2–3 months if the
by the pituitary gland. At the onset of puberty, the ovum is fertilized. By that time placenta develops and
pituitary gland secretes follicle stimulating hormone starts secreting progesterone and estrogen. The high
(FSH). Under the influence of this hormone, the pri- levels of these hormones in blood further suspend the
mordial follicles in the ovary start growing. The grow- ovarian cycle during pregnancy.
ing/maturing follicles produce hormone estrogen. N.B. The ovarian cycles normally persist throughout the repro-
Only one follicle reaches the full development and ductive life of women except during pregnancy. The ovarian cycle
forms Graafian follicle. Through feedback mechanism, terminates at menopause.
LUTEAL P
HAS
E
Corpus
Corpus luteum
albicans Cortex
Germinal
Mesovarium epithelium
Primordial follicle
Ovulation
Medulla Secondary
Primary follicles
oocyte
Maturing
secondary follicle Oocyte Graafian follicle
Secondary follicle
FOLLICULAR PHASE
Fig. 2.4 Schematic diagram of ovary showing various stages of development of ovarian follicles, and formation of corpus luteum
and corpus albicans.
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14 Textbook of Clinical Embryology
Fundus
Uterine
tube
Uterine cavity
Body
Uterine wall
Isthmus
Internal os
Supravaginal
portion of cervix Cervical canal
Cervix
Vaginal portion of
cervix
External os
Vagina Cavity of vagina
A B
Fig. 2.6 Uterus: (A) external view; (B) internal view.
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Reproductive System 15
Lining epithelium
(simple columnar, and secretory)
Stratum
compactum
Uterine
gland
Stratum
Spiral
spongiosum
artery
Stratum basale
Straight Myometrium
artery
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16 Textbook of Clinical Embryology
Ovarian cycle
Luteal phase
Menstrual cycle
Stratum
compactum
Stratum
spongiosum
Stratum basale
N.B. Changes in the endometrium occur as a result of hormones secretion of the estrogen by the maturing follicles
(estrogen and progesterone) secreted by the ovaries (ovarian cycle), of the ovary.
which in turn is controlled by hormones secreted by the hypothala-
mus and pituitary gland.
3. Secretory phase/luteal phase (15–25 days): The
secretory phase coincides with the secretion of pro-
1. Menstrual phase (menses) (1–4 days): If the gesterone by the corpus luteum.
ovum is not fertilized, the corpus luteum degener- 4. Premenstrual phase (26–28 days): The females,
ates and the level of progesterone drops down. The usually the younger ones, often complain of severe
coiled endometrial arteries undergo spasm. The spasmodic pain and external spotting of blood dur-
blood supply to the spongy and compact layers of ing this phase due to ischemia of the uterine wall
the endometrium is reduced. The functional layer following drop in the level of progesterone
undergoes necrosis and sloughs off, and there is hormone.
hemorrhage from the stumps of the endometrial The features of different phases of menstrual cycle
arteries. The sloughing continues until only raw are summarized in Table 2.1.
surface of the stratum basale is left. N.B. The menstrual cycle is a continuous process, and each phase
N.B. It takes about 14 days after ovulation in breaking down the gradually passes into the next one.
spongy and compact layers of endometrium. Note the basal layer
of endometrium remains intact.
If the ovum is fertilized, first the corpus lu- Hormonal Control of Menstrual Cycle (Fig. 2.9)
teum and then the placenta continue to secrete The menstrual cycle is controlled by the hormonal se-
progesterone, and the menstrual cycle remains cretions of hypothalamus, adenohypophysis, and ovary
suspended during pregnancy. as follows (Fig. 2.9):
2. Proliferative phase/follicular phase (5–14 days): 1. The hypothalamus secretes gonadotrophin-releas-
The proliferative phase coincides with the ing hormone (GnRH).
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Reproductive System 17
Phase Features
Menstrual phase (1–4 days) Necrosis and shedding of the functional layer of the endometrium associated with bleeding
Proliferative phase (5–14 days) Regeneration of the functional layer of the endometrium
Secretory phase (15–25 days) Endometrium becomes thick and soft due to increased secretory activity of endometrial glands
Premenstrual phase Ischemia of endometrium due to reduced blood supply
(26–28 days) Cramping or pain and external spotting of blood
Hypothalamus
GnRH
LH
Adenohypophysis FSH
Ovary
Progesterone
(ovarian cycle)
Estrogen
Ovulation Secretory
Uterus (uterine/ phase
menstrual cycle) Proliferative
Menstrual phase Premenstrual
phase phase
Days 0 4 8 12 14 16 20 24 28
2. The GnRH acts on the adenohypophysis, which in 8. The progesterone stimulates the uterine endome-
turn secretes FSH and LH. trium to enter the secretary phase.
3. The FSH causes maturation of one or more ovarian N.B. The hormones secreted by hypothalamus, adenohypophysis,
follicles. The secondary follicle is converted into and ovary prepare the endometrium of the uterus for implantation
the Graafian follicle. of the conceptus (blastocyst). If fertilization does not occur, the
4. The granulosa cells of the secondary and Graafian granulosa cells produce inhibin, a protein that acts on adenohy-
pophysis and inhibits the secretion of gonadotrophins, which leads
follicles secrete estrogen. to the regression of corpus luteum. The endometrium undergoes
5. The estrogen stimulates the uterine endometrium ischemic necrosis due to decreased levels of progesterone and
to enter the proliferative phase (the level of estro- estrogen, especially progesterone secretion by the degenerating
gen rises to a peak just before the LH surge). corpus luteum.
6. The LH surge stimulates ovulation. For details, see Chapter 3.
7. Following ovulation, the lutein cells of the corpus The ovarian and menstrual cycles go on hand in hand
luteum secretes progesterone. throughout the reproductive life of women, except during
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18 Textbook of Clinical Embryology
Site of fertilization
Sperm
Ampulla of uterine tube
Uterine tube
Ovum
(secondary oocyte)
Uterine
cavity
Ovary
Cervical canal
External os
Sperm
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Reproductive System 19
From infundibulum, the oocyte passes to the am- The male gametes (sperms) generally remain via-
pulla of the tube mainly by the peristaltic movements ble for 48 hours in the female genital tract.
of the tubal wall. N.B. According to some authorities, female and male gametes
may survive up to 2 and 4 days, respectively.
Viability of Gametes
The female gametes (mature oocytes) generally re-
main viable for 24 hours after ovulation. They are usu-
ally fertilized 12 hours after ovulation.
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20 Textbook of Clinical Embryology
CLINICAL PROBLEMS
1. When an infertile (childless) couple visits a doctor, why male fertility is evaluated first?
2. What are the causes of male infertility?
3. What is the most effective permanent method of contraception in males?
4. In some women the cause of infertility is anovulation (i.e., cessation of ovulation). Is it possible to induce ovulation
in these women?
5. How is ovulation assessed clinically?
6. What is the importance of determining the time of ovulation?
7. Which is most precarious time of prenatal development (i.e., period of greatest sensitivity to teratogens)? Give
the embryological basis.
1. This is because the semen analysis is easier to perform. The average volume of semen ejaculated in the vagina during
sexual intercourse is 2–6 mL (average 3.5 mL). There are usually more than 100 million sperms per mL of semen of
normal males. A man with less than 10 million sperms per mL of semen is likely to be sterile, especially when the
specimen contains immotile and abnormal sperms.
2. The common causes of male infertility are low sperm count (oligospermia), poor sperm motility, abnormal sperms,
and obstruction of the genital tract (e.g., vas deferens).
3. The most effective permanent method of contraception in males is vasectomy. This procedure involves the excision
of a segment of each ductus (vas) deferens. Following vasectomy, there are no sperms in the semen or ejaculate, but
the volume remains the same.
N.B. First one or two ejaculate may contain sperms.
4. In some women, ovulation does not occur due to inadequate secretion of FSH and LH. Ovulation can be induced in
such women by administrating gonadotrophins or an ovulatory agent such as clomiphene citrate. By competing
with estrogen for binding sites in the adenohypophysis, the clomiphene citrate suppresses the normal negative feed-
back loop of estrogen on the adenohypophysis. This in turn stimulates the release of pituitary gonadotrophins (FSH
and LH) secretion, which causes maturation of several ovarian follicles and thus induces ovulation.
5. Ovulation can be assessed because it is accompanied by:
(a) A variable amount of abdominal pain in some women called mittelschmerz (German 2 mittel 5 mid 1 schmerz 5
pain) because ovulation results in slight bleeding in the peritoneal cavity.
(b) A slight drop in the basal body temperature.
In a 28-day menstrual cycle, the ovulation takes place at about the middle of the cycle, to be exact on day 14 before
the start of next menstrual bleeding.
There are many methods to find out the exact time of ovulation, but the one that is easy and commonly used is the
temperature method. In this method, woman’s body temperature is recorded every morning before she gets up and
plotted on a graph. The temperature is low during menstruation, subsequently it rises, and at about the middle of the
cycle it suddenly falls to rise again. The rise in temperature after sudden fall indicates that ovulation has occurred.
Following ovulation, the basal body temperature increases by 0.3–0.5°C.
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Reproductive System 21
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Cell Division
and Gametogenesis
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Cell Division and Gametogenesis 23
Nucleolus Nucleolus
Nuclear Replication
membrane of DNA
Chromatin
threads
A B
Fig. 3.1 Cell in interphase stage: (A) early interphase; (B) late interphase.
Centriole
Spindle
fibers
Nuclear
membrane
Centromere
A B C
Prophase Prometaphase Metaphase
• Chromosome with two identical chromatids • Centrioles move to opposite poles • Nuclear membrane disappears
• Chromatids are not recognized • Chromatids become recognizable • Chromosomes line up on equator
• Are attached to the spindle fibers
Cleavage Cytokinesis
furrow
shorten, and thicken. Each chromosome now con- supra) line up in the equatorial plane of the spindle
sists of two parallel subunits called chromatids, and get attached to the microtubules of the spindle
which remain joined to each other at a narrow extending between two centrioles, one at each pole.
common region called centromere. But the chro- 4. Anaphase: In this stage, the centromere of each
matids cannot be recognized in this stage. chromosome splits and the two chromatids are
2. Prometaphase: In this stage, the chromatids separated from each other. They are now called
become distinguishable. daughter chromosomes. The spindle fibers attached to
3. Metaphase: In this stage, the nuclear membrane the centromere of the chromosomes contract and
breaks. The double structured chromosomes (vide pull the daughter chromosomes towards poles.
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24 Textbook of Clinical Embryology
Because of pull on centromere, the daughter chro- This event is called synapsis and each synaps-
mosomes become V-shaped with their arms trail- ing pair is called bivalent.
ing as they move toward the poles. (c) Pachytene: This stage is very long and may
5. Telophase: In this stage, the separated chromatids extend even for years. It is characterized by
are migrated to the opposite poles of the spindle. following changes:
The spindle fibers disappear and nuclear mem- – The chromatids of each chromosome
brane appears around each polar group of daughter become visible separately. Each bivalent
chromosomes. The chromosomes uncoil and chromosome thus appears to have four
become less compact. The nucleolus reappears. chromatids and is called tetrad. Each chro-
There appears a cleavage furrow beneath the equa- matid pair is united by a kinetochore.
tor that deepens and separates the two daughter There are two central chromatids and two
cells (cytokinesis). peripheral chromatids (one from each
chromosome).
– The two central chromatids (one belonging
Clinical Correlation to each chromosome) of tetrad coil over
each other so that they cross at a number
Significance of mitosis
of points. This is called crossing over.
1. Genetic stability: It ensures continuous succession of identi- Because of crossing over, the central chro-
cal cells through generations.
matids present a cross-like configuration
2. Growth and development: It helps in growth and develop-
called chiasmata
ment of the body.
3. Regeneration, replacement, and repair: It helps in regenera-
(d) Diplotene: During this process, the paired
tion of new cells to replace the dead or damaged cells. homologue of tetrad starts separating. The
central chromatids break at the point of cross-
ing over and unite to the opposite chromatid.
This results in exchange of genetic material
Meiosis (Fig. 3.3) between these chromatids.
(e) Diakinesis: The chromosomes become more
The meiosis is a special type of cell division that takes contracted and migrate toward the nuclear
place only in the germ cells to produce male and fe- membrane. At the end of prophase, the nuclear
male gametes. The meiosis consists of two cell divi- membrane disappears.
sions that take place one after the other. (a) First 2. Metaphase: The homologous pairs of chromo-
meiotic division (meiosis I or reductional divi- somes become arranged on the equatorial plane of
sion): In this division, the number of chromosomes of the spindle.
the daughter cells is reduced to half of the mother 3. Anaphase: In this stage, the homologous chromo-
cell. (b) Second meiotic division (meiosis II): It is somes migrate to the opposite poles of the spindle.
the mitotic division similar to one described above Unlike mitosis, the chromosomes move randomly.
except that there is no duplication of DNA during The shorter chromosomes move earlier than the
short interphase. longer chromosomes.
4. Telophase: In this stage, the nuclear membrane is
First Meiotic Division formed around the polarized group of chromo-
1. Prophase: Prophase of the first meiotic division is somes. The cell membrane constricts and two
very long and complicated. It is therefore sub daughter cells are formed (cytokinesis). Each
divided into five stages. daughter cell thus formed contains only half the
(a) Leptotene: In this stage, the chromosomes, as in number of chromosomes (haploid number) with
mitosis, appear as slender threads. Note: exchanged genetic material.
Although each chromosome consists of two
chromatids that are joined at centromere, the Second Meiotic Division
chromatids are not visible at this stage. The second meiotic division is essentially similar
(b) Zygotene: In this stage, the lengthwise pairing to mitosis. It, however, differs from mitosis in that
of homologous chromosomes begins. One of the the DNA does not duplicate. In second meiotic divi-
two homologous chromosomes is from the sion, the two daughter cells of first meiotic division
father (paternal chromosome) and the other is form four daughter cells, each with haploid number
from the mother (maternal chromosome). of chromosomes.
mebooksfree.com
Cell Division and Gametogenesis 25
A B C D E
Leptotene Zygotene Pachytene Diplotene Diakinesis
• Chromosomes appear • Pairing of homologous • Four chromatids • Crossing over • Chromosomes
as slender threads chromosomes (bivalent) become visible (synapsis of two after genetic
• Each chromosome (tetrad) central chromatids) exchange migrate
consists of two • Formation of towards the nuclear
chromatids chiasmata membrane
Praphase
• Formation of spindles
• Homologous chromosomes
get arranged on the equatorial
plane
Metaphase
Fig. 3.3 Meiotic divisions I and II: (A, B, C, D, and E) showing five stages of prophase of first meiotic division.
Clinical Correlation
material during crossing over in the meiosis, the daughter
Significance of meiosis cells (i.e., gametes) have a new genetic configuration. This
1. Maintenance of normal chromosomal number: As the chro- causes individual variations within the species, which is
mosome number is reduced to half during meiosis, each germ essential for evolution.
cell has haploid number of chromosomes. When two germ cells 3. Hybrid vigor: Meiosis helps to maintain vigor in progeny
unite to form a zygote, the chromosome number is restored to through sexual reproduction.
normal (diploid number of chromosomes). Thus, because of
meiosis, the chromosome number is maintained for the species.
2. Genetic variation: Because of random assortment of pater-
nal and maternal chromosomes, and exchange of genetic The distinguishing features between mitosis and
meiosis are given in Table 3.1.
mebooksfree.com
26 Textbook of Clinical Embryology
Daughter cells are identical Daughter cells are not The steps of spermatogenesis are summarized in
to each other and to the identical to each other and to Flowchart 3.1.
parent cell the parent cell
To understand the process of spermiogenesis, the
Equational division Reductional division student must first understand the structure of sperma-
tozoon (Fig. 3.5).
mebooksfree.com
PGC
Primordial
44XY
germ cell (PGC)
44XY
Mitosis
Spermiogenesis
Fig. 3.4 Spermatogenesis. Figure in the inset on the right shows spermatocytogenesis.
27
mebooksfree.com
28 Textbook of Clinical Embryology
Cell membrane
Acrosomal cap
Head Nucleus
Cell membrane
Neck Basal plate
Proximal centriole
Middle
piece Cell membrane
Mitochondrial sheath
Tail Principal
Cell membrane
piece
Fibrous sheath
Seven outer dense fibers
Axial filament
Fig. 3.5 Human sperm. The parts of mature sperm are shown on the left side, whereas the sections through the head, neck,
middle piece, principal piece, and end piece along with their composition are shown on the right side.
Head The head of sperm appears somewhat like a 3. End piece: It is made up of only the axial filament.
spearhead in section. It mainly consists of a nucleus
N.B.
that contains the condensed chromatin material (mostly l Structure of the axial filament is very similar to that of the
Tail The tail consists of three parts: middle piece, N.B. The axial filament is responsible for the movements of the
spermatozoon, while mitochondria supply energy for these
principal piece, and end piece. movements.
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Yet of all the pictures of either the stately or the modest homes of
Scotland, which now hang in the gallery of memory, none exceeds in
beauty and charm those of the home at Invergowrie, where we were
several times guests. Here was a typical Scottish family of character
and culture. Father and mother were in the prime of life, health, and
manifold activities. Around them had grown up a family of sons and
daughters, from the youngest, a blooming maiden of eighteen,
educated in Germany and at Brussels, to the eldest son, who,
besides being active in business, was an officer in the local volunteer
artillery corps. At night he loved to put on his Highland suit for
comfort and enjoyment, as we chatted with his friends on British and
American politics. One of these talks, in the billiard-room, was soon
after President Cleveland had issued his strenuous proclamation
concerning Venezuela, when British feelings were hurt and when a
combination of tact, some knowledge of history, and of the political
and personal motives of American Presidents was necessary to the
guest and peacemaker.
The Invergowrie family honored the American in Scotland more
than once by inviting, to the dinners given in his honor, the
professional gentlemen of the neighborhood and from Dundee.
While in Scotland one must beware of what toes he is likely to
tread upon, should he nurse opinions differing from those welcomed
by people holding any of the various shades of Presbyterianism,
whom he will probably meet anywhere and everywhere. It was in
Scotland, above every other country, that we learned what it meant
to “mind your p’s,” yet our hope is that we succeeded measurably.
The garden parties, in which the young people had their fun and
amusement, the five o’clock teas, at which the ladies of the
neighborhood dropped in for chat and friendly calls, were as
delightful to enjoy as they are now pleasant to recall. Yet, as in the
United States the county fair excels all other inventions and facilities
for seeing the real, average American, so I valued most, for intimate
knowledge of the Scottish populace of all grades and ages, the local
exhibitions, “bazaars,” and gatherings.
To be present, as we often were, to see the modern version of
“The Cotter’s Saturday Night,”—though in this version we mean a
luxurious home, with all the appointments of comfort, culture, and of
service,—crowned all delights. This social situation in Scotland is
necessarily different from that in America, where, in the cities at
least, native-born maidens so rarely take domestic service in
families. With our composite people, also, there is usually such a
disagreement as to the theories of the universe, as taught by priest,
parson, and rabbi, that worship of the same God by all, at one time,
in the same way, and in one household, seems impossible. The
head of the house or the mistress holds usually to one form of
dogma or ritual, while the servants have been reared in an
atmosphere so very different that family worship, with the “help”
joining in or present, is, to say the least, not customary in “the land of
the free.” Occasionally British visitors, who imagine that the very
mixed people called Americans are descended chiefly from insular
instead of continental stock, like the late James Anthony Froude
when in New York city, for instance, make disagreeable discoveries.
At times, in our democracy the kitchen rules the parlor.
At Invergowrie, as in a score or more of homes on the island in
which I have been so often a guest, the two or three maids and
perhaps a man-servant came in with their Bibles and read, with the
children of the household, the Word which is above every other word
—the Father’s message to his children. Where there was but one
servant, the same rule usually held. At Invergowrie, besides a
chapter of Scripture and a prayer by the father, the high priest of the
family, the older son read one of the Psalms in Rous’s metrical
version.
The breakfast in Scotland, as in the British Isles generally, is one
that suits admirably the free-born Briton. It is certainly a festival of
freedom for the servants, who are usually apt to be upstairs or
attending to other domestic duties, though in a large family, the
members of which sit down at the same time, there is usually one
maid present to wait upon the table. At several places where I was
entertained, even in well-to-do families, the grown sons and
daughters or members of the household came and went at their
convenience, helping themselves at will from dishes on a sideboard.
After the table has been laid by one of the maids, who may or may
not remain present, it may be that the elder daughter serves. At
Invergowrie there was a large, normal family, consisting of parents
and children, with sufficient uniformity of dispositions and habits to
make both the breakfast and the dinner time a delightful gathering,
with merriment and leisure. The news of the day, the happenings of
the neighborhood, the things alike and different as between Scotland
and America, the annals of the village fair, or the social chat, or
those pleasant nothings that lubricate life, made the moments pass
all too rapidly.
That father benign and mother of imposing presence have been
long laid to sleep; but in London, and Edinburgh, and Dundee, in the
world at large, live yet these sons and daughters of “Bonnie
Scotland” who have made the Americans’ memories of their lovely
home in their home land a storehouse of delight.
Besides the private grounds of the home, with their trees and
shrubbery, there were walks that afforded plenty of room for rambles.
Still farther afield, yet not far away from either the house or the
railway station, were ruins of Dargie Church. These touched the
imagination and called history to resurrection. It appeared strange to
come across the footprints of our old friend St. Winifred, or Boniface,
whom we met with in our studies of the Pilgrim Fathers, and in the
Netherlands, whose varied and strenuous life, as ecclesiastical
politician as well as saint and soldier of the Papacy, quite as much
as preacher of the gospel, was one of such amazing activity. At
Scrooby in England, at Dokkum in Friesland, in France and in
Germany, where I visited the places made historic by his activities,
he left enduring marks of his influence and power.
At Invergowrie I meditated among the ruins of the old kirk, in
which, or near by, it is said, St. Boniface, the apostle to Germany
and a legate from the Church of Rome, in the eighth century
preached and planned to neutralize the work of the Irish monks in
favor of British uniformity, and by means of conformity with Rome.
Here also are still to be seen some singular examples of ancient
sculptured stone monuments.
In 1107, Alexander I, son of Margaret of England, had a
residence at Invergowrie, which, however, he did not long possess,
for assassination was so much of a pastime with many and a settled
custom with a few in those days, that, after having escaped the dirk
only by a narrow margin, he left Invergowrie, built a church at Scone,
and then turned over the property he left behind him for its support.
I recall that it was at the last of our visits and entertainment at
Invergowrie, which was in 1900, after the ladies had left the dinner
table and the gentlemen adjourned to the billiard-room for a smoke,
the conversation turned on the next European war, and the possible
relations of Great Britain and the United States in the alignment of
friends, foes, allies, and neutrals. One prominent Dundeean
confessed himself not so much exasperated, as hurt, by President
Cleveland’s sharp method of reasserting the Monroe Doctrine, in
regard to the boundary of Venezuela. From our town of Ithaca, the
two scholars, the ex-president of Cornell University and Professor
George Burr, had been summoned to consult archives, rectify
boundaries, and help keep the peace. After the American in Scotland
had emptied his cruse of oil upon the waters, by explaining some of
the ins and outs of American politics, the conversation drifted to
regions across the North Sea—the growth of the Kaiser’s navy, the
salient features of German politics, and the reports, then very direct
from the Fatherland, that the “Kultur” of the twentieth century
required that “England [Great Britain] needed to be taught a lesson.”
The hope was warmly expressed that, in the coming clash,—then
looked for to come before many years,—the sympathy, and even aid,
if necessary, of “the States” would be forthcoming. With American
friendliness and the possession of the coaling-stations of the world, it
was believed that the United Kingdom could withstand the coming
shock and recover triumphantly.
More than once, at these social conferences with Scotsmen, as
well as in the press, I noted the indignation, even anger, expressed,
that in all national affairs it was “England” and “the English” that took
and received the credit for what belonged to the four nations making
up the United Kingdom. The claim was for a more liberal use of
“Britain” and “British” in place of “England” and “English.” Both
Scotch and Irish, to say nothing of the Welsh, resent the assumption
that “England” is the British Empire. In a word, the great need of the
language used in the British archipelago is a common name for the
federated four countries and for all the subjects of the Crown. Here is
an instance of the priceless value of right words. The absence of an
acceptable comprehensive term is a real impediment to patriotism
and an obstacle to perfect union. The fault is in language, not in the
human spirit. The situation reinforces the argument that “words are
things.”
We Americans can throw no stones. Canadians, Mexicans, the
southern republics below Panama, all challenge our right to the
monopoly of “America” and “Americans.” Language lags behind
events.
When at last, in 1914, the great war did come, and the storm
broke, no part of the Empire responded more quickly, generously,
fully than Scotland, nor did any courage or sacrifice exceed that of
the Scots; yet, not only was the credit usually given to “England,” but
even the prayer of hate, made in Germany, chose “England” as its
butt. Yet while Scottish valor and sacrifice and Irish courage and
free-will offerings of life on the field and waves are unstinted, who
can blame the poet, nay, who does not say “amen,” to his lines, in
the Glasgow “Herald,” written in the closing days of 1915?
PREHISTORIC
Britain, “north of the Tweed.” Picts and various
tribes.
CHRISTIAN SCOTLAND
563. St. Columba (521–592), Christian missionary at
Iona.
573. St. Kentigern at Glasgow.
651. St. Cuthbert at Melrose.
710. The Pict Christians conform to the Roman
Church rules.
717. The Columba monks expelled.
730–761. The Pict, Angus MacFergus, paramount.
802. Iona burnt by the Norsemen. Desolate for two
hundred years.
802–839. The Scandinavian sea-rovers settle on the
northern coasts.
844–860. Kenneth MacAlpine, King of the Picts.
Blending of the Picts and Scots into one people.
904. St. Andrews: religious centre. Stone of Scone.
945. Malcolm acquires northern Strathclyde.
1018. Lothian part of the Celto-Pict realm.
1005–1034. King Malcolm II.
FEUDAL SCOTLAND
1039–1056. Macbeth flourishes.
Ireland, “the Land of the Scots,” is known by its
modern name. “Scotland” refers to northern
Britain.
1057. Macbeth defeated and slain by Malcolm
Canmore.
1066. Normans invade England.
1058–1093. Malcolm Canmore and Queen Margaret.
Great social and political changes in Scotland.
The Celtic Church gives way to Western
uniformity.
Dunfermline, capital of the realm.
1124. Alexander, King of Scotland.
Planting of Norman, Flemish, and Anglican
colonies on east coast.
Anglo-Norman feudalism in Scotland.
David I, “The Maker of Scotland,” builder of
abbeys and bishoprics.
1153–1165. Malcolm the Maiden. Great Clan of Macdonalds
formed.
Ascendancy of Anglican influence. Inverness
granted a royal charter.
1165–1214. William the Lion. Dundee granted a royal
charter.
Chimneys introduced into Scotland.
1249–1286. Alexander III. Treaty with Norway.
Islands incorporated in the Scottish realm.
1292. John Baliol crowned on the Stone of Scone.
THE RENAISSANCE
1460–1488. James III marries Anne of Denmark.
The thistle, the national badge of Scotland.
Witchcraft. King imprisoned by the nobles and
assassinated.
1465–1536. Hector Boece writes the “History of Scotland.”
1488–1513. James IV. Modern History of Scotland begins.
1494. Grey Friars’ Church in Edinburgh built.
Ayala, Spanish envoy and writer on Scotland.
Music and poetry cultivated.
1495. University of Aberdeen founded.
1496. Parliament decrees compulsory education.
University of St. Andrews. Hepburn founds St.
Leonard’s College.
1503. Marriage of James IV with Margaret of England,
at Holyrood.
First Peace with England since 1332. An era of
prosperity.
1505. Royal College of Surgeons founded at
Edinburgh.
1507. Printing introduced into Scotland.
1513. Battle of Flodden Field.
THE REFORMATION
Rise of the burgesses and middle classes.
1513–1542. James V: minority. Angus rules. James escapes
to France.
1537. James marries Mary of Guise, and on her
decease, Mary of Lorraine.
1540. Lordship of the Isles annexed to the Crown.
1542. Invasion of Scotland by Henry VIII.
King and clergy on the Roman, nobles on the
Reformed, side.
1542–1587. Mary Stuart, Queen of Scots.
Close relations with France.
1505–1572. John Knox.
Destruction of monasteries and abbeys.
1557. Last Protestant martyr burned.
1565. Queen Mary marries Lord Darnley.
1566. Murder of Rizzio in Holyrood.
1567. Murder of Lord Darnley.
Marriage of Mary with Bothwell.
1567–1625. George Buchanan, scholar, reformer, author of
De Jure Regni apud Scotos.
James VI educated by George Buchanan.
PRESBYTERIAN SCOTLAND
1560. Foundation of the National Church.
First General Assembly of Scotland.
1578. Andrew Melville the Reformer. Second Book of
Discipline.
Divine Right of Presbytery taught. Nobles
debarred from spoiling the Church.
1587. Execution of Mary Queen of Scots.
1592. James gives Presbyterianism his sanction.
1603. Union of the crowns of England and Scotland.
James VI of Scotland becomes James I of
England.
1605. The Border region pacified and civilized.
1606. The Union Jack flag, uniting crosses of St.
George and St. Andrew.