NKP SALVE INSTITUTE OF MEDICAL SCIENCES & RESEARCH CENTRE, NAGPUR

SUBJECT :- BIOCHEMISTRY
Ist MBBS 2022 Batch
Total Duration 3 hrs Time: 10.30 to 1.30
Terminal Exam Date:- 22/02/2023
Section A - MCQ 20 Marks (20 x 1 Marks = 20)
1. The Golgi complex
A) Synthesizes proteins B) Produces ATP
C) Provides a pathway for transporting chemicals D) Forms glycoproteins

2. A vasodilating compound is produced by the decarboxylation of the amino acid:

A) Aspartic acid B) Histidine C) Glutamine D)Arginine

3. Which of the electron carrier is soluble & Mobile?

A) Cytochrome a B) Cytochrome C C) CoQ D) Cytochrome b

4. The limiting amino acid of rice is

A) Lysine B) Tryptophan C) Phenylalanine D) Tyrosine

5. Isomers differing as a result of variations in configuration of the —OH and —H on carbon atoms 2, 3 and 4 of
glucose are known as
A) Epimers B) Optical isomers C) Anomers D) Steroisomers

6. Which of the following organelle have DNA

A) Peroxisome B) Mitochondrion C) Lysosomes D)Microsome

7. Electrophoretic separation of protein is of diagnostic value in all condition except

A) Nephrotic Syndrome B) Multiple Myeloma
C) Alpha 1 Antitrypsin deficiency D) Aquired Immunodeficiency disorder.

8. The glycosaminoglycan which does not contain uronic acid is

A) Dermatan sulphate B) Chondroitin sulphate
C) Keratan sulphate D) Heparan sulphate

9. The rate limiting step in heme synthesis is

A) Heme Synthase B) ALA dehydratase C) ALA synthase D) ALA dehydratase

10. Cori’s cycle transfers

A) Glucose from muscles to liver B) Lactate from muscles to liver
C) Lactate from liver to muscles D) Pyruvate from liver to muscles

11. Sanger’s reagent contains

A) Phenylisothiocyanate B) Dansyl chloride
C) 1-Fluoro-2, 4-dinitrobenzene D) Ninhydrin

12. A compound serving a link between citric acid cycle and urea cycle is
A) Malate B) Citrate C) Succinate D) Fumarate

13. Serotonin is synthesised from

A) Tryptophan B) Phenylalanine C) Tyrosine D) Serine

14. Enzymes synthesized in inactive forms are known as

A) Coenzyme B) Apoenzyme C) Proenzyme D) Isosozyme
15. Transketolase has the coenzyme:
A)TPP B)FAD C)NAD+ D) PLP

16. A newborn baby had severe abdominal distension, severe abdominal pain, and diarrhea after being fed breast
milk. Urine analysis revealed the presence of reducing sugar. What is the possible defect?
A) Diabetes Mellitus B) Galactosemia
C) Malabsorption Syndrome D) Lactose Intolerance

17. Galactosyl transferase is the marker enzyme of

A) Cell membrane B) Mitochondria C) Lysosomes D) Golgi complex

18. A newborn baby presented with yellowish discoloration of skin & conjunctiva after 3 days of birth. Neonatologist
advised photo therapy for the child. What can be reason for above condition?
A) ABO incompatibility B) Prematurity
C) Inefficient conjugating system at liver D) All of the above

19. Which of the following is a physiological uncoupler of electron transport chain?

A) Thyroxine B) Glucagon c) Glycerol 3 phosphate d) Insulin

20. A 20 years old man came to an emergency room with severe pain in his right side & back. Clinical evaluation
indicated a kidney stone & increased excretion of cystine, arginine, ornithine & lysine in urine. What is your
diagnosis?
A) Hartnups Disease B) Homocysteinuria C) Oochronosis D) Cystinuria.
 Section B
Q. 2 Brief Answer Questions (Any Ten out of Eleven) (10x2=20)
1 Give two examples of antiport system.
2. What is glycosylated Hb? Write its significance
3. Name two proteins active in Quaternary structure with its components.
4. Significance of 2,3 Bisphosphoglycerate.
5. Define Km? What is its significance.
6. First step of urea cycle.
7. Write down principle of colorimetry & its application.
8. Enumerate 4 products formed from Glycine.
9. Define Conjugated proteins with 2 examples.
10. What is anaplerosis? Mention one anaplerotic reaction.
11. Name any 2 high energy compounds.
12. Define transmethylation reaction with two compounds produced by them.

Q. 3 Short Answer Questions (Any Eight out of Nine) (8x5=40)

1) Draw schematic diagram of Electron Transport chain with site of phosphorylation & Inhibitors.
2) Biological membrane fluid mosaic model.
3) Nutritional classification of Amino acid. Write a note on Denaturation.
4) Significance of Dietary fibers.
5) Competition inhibition of Enzyme.
6) Write the structure & function of hemoglobin. Add a note on haemoglobinopathies.
7) A 30 years male patients was suffering from malaria and was put on drug Primaquine. During the treatment, he
developed signs of anaemia (Pallor, fatigue, breathlessness). He gave history of dark coloured urine since the episode
of illness. Laboratory tests indicate reduced blood hemoglobin levels and the liver enzymes. (Sr. AST & ALT)
showed mild rise.
i) What is the probable diagnosis? Justify. 2M
ii) Explain the biochemical basis of the disorders with suitable reactions. 2M
iii) What are the investigation required to diagnose this condition? 1M
8) Describe different type of biomedical waste in clinical biochemistry laboratory & its disposal. Add a note on
disposal of COVID waste.
9) A patient was brought to the casualty with symptoms of myocardial infarction like chest pain & profuse sweating.
An ECG was done which had some variation but not conclusive.
i) What are the different biomarkers to be estimated on an emergency basis? 1M
ii) Draw the diagram showing pattern of enzymes expected in MI. 2M
iii) Explain importance of Isoenzyme analysis in the diagnosis of the disease. 2M

Section C
Q. 4 Long Answer Questions (Any Two out of Three) (2x10=20)
1) Describe metabolic pathway of phenyl alanine through Tyrosine along with its disorders.
2) Give an account on Glycogen metabolism with its regulation pathway. Add a note on Glycogen storage disorders.
3) Define & Classify Enzymes with suitable examples. Add a Note of Coenzymes.
 NKP SALVE INSTITUTE OF MEDICAL SCIENCES & RESEARCH CENTER AND LATA
MANGESHKAR HOSPITAL NAGPUR
SUBJECT :- BIOCHEMISTRY
Terminal Exam Date :- 22/02/2023
20 Marks
Instructions
1) Fill (Dark) the empty circle of your choice completely below the question number in the answer sheet with
Blue/Black ball point pen only.
2) Double entry i. e. Darkening more than one circle or even putting dot on other vacant circle, or ticking shall
not be considered as valid method and marks will not be allotted.

ANSWER SHEET FOR MCQ

Roll
Answers sheet for MCQ
No.
0 O O O Q. A B C D
1 O O O 1 O O O O
2 O O O 2 O O O O
3 O O O 3 O O O O
4 O O O 4 O O O O
5 O O O 5 O O O O
6 O O O 6 O O O O
7 O O O 7 O O O O
8 O O O 8 O O O O
9 O O O 9 O O O O
10 O O O O
Sign :-
11 O O O O
12 O O O O
13 O O O O
14 O O O O
15 O O O O
16 O O O O
17 O O O O
18 O O O O
19 O O O O

20 O O O O
Total Marks :-

Sign of Teacher:-