Biochemistry : LIPID METABOLISM (QUIZ 4)

NAME: 1. TOTAL
2. Group #: SCORE:
3.
CODE & SET: DATE SUBMITTED:
INSTRUCTOR:

Instructions:
 Read the question and the instructions carefully.
 Write legibly, STRICTLY NO super impositions, NO erasures and DO NOT use friction pen or
pencil.

I. FILL IN THE BLANKS. (1pt ea)

1. Digestion in mouth: A lipase has been detected called ______________ which is secreted by the
dorsal surface of the tongue.
2. Digestion in stomach: Gastric lipase is acid stable, with an optimum pH about 5.4. It is secreted by
____________, the secretion is stimulated by ___________.
3. The major site of fat digestion is the ______________. This is due to the presence of a powerful
lipase in the pancreatic juice and presence of bile salts, which acts as an effective ____________ for
fats.

II. WORDS IN A BOX choose a word inside the box to answer the following questions. You are
allowed to use the word inside the box multiple times. (1pt ea)
A. SIX STEPS OF LIPID ABSORPTION:
1. _______________ of triacylglycerols in mouth and stomach by lingual (acid-stable) lipase.
2. _______________ facilitates formation of mixed micelles
3. _______________ of the products of lipolysis from the mixed micelle into the intestinal epithelial cell
4. _______________ of all lipids in the lumen of the duodenum/ jejunum by pancreatic lipolytic enzymes
5. _______________containing Apo B48, triacylglycerols, cholesterol esters and phospholipids and
export from intestinal cells to the lymphatics.
6. _______________ of 2-monoacylglycerol with free fatty acids inside the intestinal enterocyte

Minor digestion Bile acid Re-esterification

Major digestion Passive absorption Assembly of chylomicrons

B. BETA-OXIDATION CONSIST OF FOUR STEPS:

1. ______________ catalyzed by acyl-CoA dehydrogenase, which removes two hydrogens between
carbons 2 and 3.
2. ______________ catalyzed by enoyl-CoA hydratase, which adds water across the double bond.
3. ______________ catalyzed by 3-hydroxyacyl-CoA dehydrogenase, which generates NADH.
4. ______________ catalyzed beta-ketothiolase, which cleaves the terminal acetyl-CoA group and forms
a new acyl-CoA which is two carbons shorter than the previous one.

Dehydrogenation Thiolytic cleavage Hydroxylation

Hydration Dehydration Recombination

III.