UNIVERSITY OF THE PUNJAB Roll No. ……………..

BS 4 YEARS PROGRAMME (SIXTH SEMESTER) FALL 2023

PAPER: BOTANY LAB (GENE CLONING) TIME ALLOWED: 3 Hrs
COURSE CODE: BOT – 314 MAX MARKS: 100

GROUP - I

Q.1: Give short answers to the following questions: 5x6 = 30

a. What is the difference between Transformation and Conjugation?
b. Define DNA methylation and how it effects the plasmid digestion by restriction enzymes?
c. Enlist and explain the main reagents, required for plasmid extraction through alkaline lysis.
d. How to prevent the plasmid vector from self-ligation?
e. What is the principle of gel electrophoresis?
Q.2: How is agarose gel electrophoresis used for the detection of Plasmid DNA? 25
Q.3: Describe the process of Total cell lysate preparation? 25

Q.3: Practical Note Book 10

Q.4: Viva Voce 10