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1. Title Page: should include the title, name of each author, and the
institution at which the work was performed.
Text should be centered.
DWA3, an Arabidopsis DWD protein, acts as a negative
regulator in ABA signal
transduction

Jae-Hoon Lee, a William Terzaghi,a,b and Xing Wang Deng a,*

Department of Molecular, Cellular, and Developmental Biology, Yale

University, New Haven, Connecticut 06520-8104, U.S.A.
b
Department of Biology, Wilkes University, Wilkes-Barre, PA 18766,
U.S.A.

*
Corresponding author, Fax: +1 203 432 3204
E-mail address: xingwang.deng@yale.edu

Keywords: CUL4-based E3 ligase; DWD; ABA; negative regulator;

signal transduction pathway; Arabidopsis
a

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2. Abstract: summarizes the paper in 250 words or less.
DWD proteins have been reported as substrate receptors for cullin
RING ubiquitin ligase 4 (CRL4). Upon screening T-DNA mutants of
DWD genes for abscisic acid (ABA) responses we obtained several
candidates which exhibited ABA-hypersensitivity and one was named
DWA3 (DWD hypersensitive to ABA 3). DWA3 associated with the
CRL4 components DDB1 and CUL4, indicating that DWA3 may
function as a substrate receptor for CRL4. ABA-inducible transcription
factors (ABI5 and AtMYC2) and their downstream genes were hyperinduced by ABA in dwa3. Taken together, we suggest DWA3 is a
negative regulator of ABA responses and may be involved in protein
degradation mediated by CRL4.

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2. Abstract: summarizes the paper in 250 words or less.
mini-papers that are often published separately
DWD proteins have been reported as substrate receptors for cullin
RING ubiquitin ligase 4 (CRL4). Upon screening T-DNA mutants of
DWD genes for abscisic acid (ABA) responses we obtained several
candidates which exhibited ABA-hypersensitivity and one was named
DWA3 (DWD hypersensitive to ABA 3). DWA3 associated with the
CRL4 components DDB1 and CUL4, indicating that DWA3 may
function as a substrate receptor for CRL4. ABA-inducible transcription
factors (ABI5 and AtMYC2) and their downstream genes were hyperinduced by ABA in dwa3. Taken together, we suggest DWA3 is a
negative regulator of ABA responses and may be involved in protein
degradation mediated by CRL4.

MS
2. Abstract: summarizes the paper in 250 words or less.
mini-papers that are often published separately
should have 1 or 2 sentences of Introduction
DWD proteins have been reported as substrate receptors for cullin
RING ubiquitin ligase 4 (CRL4). Upon screening T-DNA mutants of
DWD genes for abscisic acid (ABA) responses we obtained several
candidates which exhibited ABA-hypersensitivity and one was named
DWA3 (DWD hypersensitive to ABA 3). DWA3 associated with the
CRL4 components DDB1 and CUL4, indicating that DWA3 may
function as a substrate receptor for CRL4. ABA-inducible transcription
factors (ABI5 and AtMYC2) and their downstream genes were hyperinduced by ABA in dwa3. Taken together, we suggest DWA3 is a
negative regulator of ABA responses and may be involved in protein
degradation mediated by CRL4.

MS
2. Abstract: summarizes the paper in 250 words or less.
mini-papers that are often published separately
should have 1 or 2 sentences of Introduction
1- 2 sentences of Materials and Methods
DWD proteins have been reported as substrate receptors for cullin
RING ubiquitin ligase 4 (CRL4). Upon screening T-DNA mutants of
DWD genes for abscisic acid (ABA) responses we obtained several
candidates which exhibited ABA-hypersensitivity and one was named
DWA3 (DWD hypersensitive to ABA 3). DWA3 associated with the
CRL4 components DDB1 and CUL4, indicating that DWA3 may
function as a substrate receptor for CRL4. ABA-inducible transcription
factors (ABI5 and AtMYC2) and their downstream genes were hyperinduced by ABA in dwa3. Taken together, we suggest DWA3 is a
negative regulator of ABA responses and may be involved in protein
degradation mediated by CRL4.

MS
2. Abstract: summarizes the paper in 250 words or less.
mini-papers that are often published separately
should have 1 or 2 sentences of Introduction
1- 2 sentences of Materials and Methods
3-4 sentences of Results, including quantitative data

MS
2. Abstract: summarizes the paper in 250 words or less.
mini-papers that are often published separately
should have 1 or 2 sentences of Introduction
1- 2 sentences of Materials and Methods
3-4 sentences of Results, including quantitative data
1-2 sentences of Discussion

MS
2. Abstract: summarizes the paper in 250 words or less.
mini-papers that are often published separately
should have 1 or 2 sentences of Introduction
1- 2 sentences of Materials and Methods
3-4 sentences of Results, including quantitative data
1-2 sentences of Discussion
rarely cite references. If they do, full citation must be included.

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3. Introduction: explains why the experiment was done.

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3. Introduction: explains why the experiment was done.
provides enough detail about what was previously known to explain
what the outstanding questions are

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3. Introduction: explains why the experiment was done.
provides enough detail about what was previously known to explain
what the outstanding questions are
specifically states the hypothesis that was tested.

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3. Introduction: explains why the experiment was done.
provides enough detail about what was previously known to explain
what the outstanding questions are
specifically states the hypothesis that was tested.
I write it last, to guide readers to my results

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4. Materials and Methods: provide sufficient detail that another
biologist could repeat your experiment.

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4. Materials and Methods: provide sufficient detail that another
biologist could repeat your experiment.
list the organisms used (including the latin binomial) the names of the
reagents, procedures followed, etc

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4. Materials and Methods: provide sufficient detail that another
biologist could repeat your experiment.
list the organisms used (including the latin binomial) the names of the
reagents, procedures followed, etc
do not give the recipe for each reagent.
Instead, cite the reference from which the recipe was obtained.

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4. Materials and Methods: provide sufficient detail that another
biologist could repeat your experiment.
list the organisms used (including the latin binomial) the names of the
reagents, procedures followed, etc
do not give the recipe for each reagent.
Do cite the manufacturers of esoteric reagents or equipment

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4. Materials and Methods: provide sufficient detail that another
biologist could repeat your experiment.
list the organisms used (including the latin binomial) the names of the
reagents, procedures followed, etc
do not give the recipe for each reagent.
Do cite the manufacturers of esoteric reagents or equipment
Both Materials and Methods and Results should be written in the past
tense. Routine calculations are not described here, unless they are done
in an unusual way.

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5. Results: devote one paragraph to each figure or table

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5. Results: devote one paragraph to each figure or table
start with a sentence explaining the purpose of the expt

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5. Results: devote one paragraph to each figure or table
start with a sentence explaining the purpose of the expt
second sentence should summarize the methods

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5. Results: devote one paragraph to each figure or table
start with a sentence explaining the purpose of the expt
second sentence should summarize the methods
third sentence states where the results are presented

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5. Results: devote one paragraph to each figure or table
start with a sentence explaining the purpose of the expt
second sentence should summarize the methods
third sentence states where the results are presented
remaining sentences point out the key features.

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5. Results: devote one paragraph to each figure or table
start with a sentence explaining the purpose of the expt
second sentence should summarize the methods
third sentence states where the results are presented
remaining sentences point out the key features.
Results can be presented as figures or tables, which should be presented
on separate pages attached after the literature cited

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5. Results: devote one paragraph to each figure or table
start with a sentence explaining the purpose of the expt
second sentence should summarize the methods
third sentence states where the results are presented
remaining sentences point out the key features.
Results can be presented as figures or tables, which should be presented
on separate pages attached after the literature cited
each figure or table should have its own title and caption

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5. Results: devote one paragraph to each figure or table
start with a sentence explaining the purpose of the expt
second sentence should summarize the methods
third sentence states where the results are presented
remaining sentences point out the key features.
Results can be presented as figures or tables, which should be presented
on separate pages attached after the literature cited
each figure or table should have its own title and caption
caption gives sufficient information that a reader can figure out what it
is about without reading the text. i.e. summarizes M&M & identifies
panels, symbols, etc

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6. Discussion: one paragraph per figure or table + a global discussion at
end

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6. Discussion: one paragraph per figure or table + a global discussion at
end
First sentence summarizes results

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6. Discussion: one paragraph per figure or table + a global discussion at
end
First sentence summarizes results
Remaining sentences explain them, and may propose ways to test these
explanations

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6. Discussion: one paragraph per figure or table + a global discussion at
end
First sentence summarizes results
Remaining sentences explain them, and may propose ways to test these
explanations
Last paragraph discusses global implications: now that we know this,
how does it change our world?

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7. Literature cited: all citations listed in the text should be listed at the
end of the paper.

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7. Literature cited: all citations listed in the text should be listed at the
end of the paper.
Formats for citing and listing references vary among journals.

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7. Literature cited: all citations listed in the text should be listed at the
end of the paper.
Formats for citing and listing references vary among journals.
Use the format (Smith, 2008) in the text

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7. Literature cited: all citations listed in the text should be listed at the
end of the paper.
Formats for citing and listing references vary among journals.
Use the format (Smith, 2008) in the text
Use this format in the Literature Cited: Smith, E.J. (2008) BRF3
encodes a novel ubiquitin ligase. Molecular Plant 3: 345-361

Manuscript Draft Grading Checklist

1) Abstract (6 pts)
Were all elements (I, M&M, R & D) present?
Were all elements adequately explained?
Was all information relevant?
Was all information clearly and succinctly explained?
2) Introduction (6 pts)
Was the hypothesis (or purpose) clearly stated?
Was adequate background information provided?
Was all information relevant?
Was all information clearly explained?
3) Materials and Methods (4 pts)
Were all procedures clearly and accurately explained?
Was all information relevant?

4) Results (10 pts)

Was there a separate paragraph for each expt?
Did the first sentence of each para state the purpose?
Did the second sentence summarize the methods?
Did the third sentence state where the results are?
Did the remaining sentences explain the figure/table and point
out
key results?
Did figures and tables do the job?
Titles and captions?
5) Discussion (8 pts)
Were key results summarized?
Were key results discussed?
Were further experiments proposed?
Were broader implications discussed?

6) Literature cited (2 pts)

Were references used correctly in the text?
Were all citations made in text listed in correct format?
Were any references not cited in the text?
7) Writing (4 pts)
Organization
Clarity
Conciseness
Grammar and spelling