ENZYME KINETIC

(WEEK 3)
 XI. ENZYME KINETIC
Rate of ES formation = k 1 (E) (S)
Rate of ES dissociation = k-1 (ES) + k 2 (ES)
Steady state = d(ES)/dt 0 k1
k1 (E) (S) = k-1 (ES) + k 2 (ES) E+S ES k2 E+P
k -1
(E) = (Eo ) - (ES)
k1 {(Eo) - (ES)} (S) = k -1 (ES) + k 2 (ES) Y=V
k1 (Eo) (S) - k 1 (ES) (S) = k -1 (ES) + k 2 (ES) VM
k1 (Eo) (S) = k -1 (ES) + k 2 (ES) + k 1 (ES) (S)
k1 (Eo) (S) = {(k -1 + k 2 + k 1 (S)}(ES) VM
k1 (Eo) (S) Eo (S) Eo (S)
(ES) = = = 0 X=S
k-1 + k 2 + k 1 (S) (k-1+ k 2 )/k1 + (S) k M + (S) S=KM
kM = Michaelis -Menten constant
k2 (E O) (S) Michaelis-Menten equation
V= d(P)/dt = -d(ES)/dt = k2 (ES) =
kM + (S) V M (S)
Vmax (VM) bila (S) >>> k M + (S) (S) VM = k 2 (E O) V=
k M + (S)

1 KM (S) KM 1 1 Y=1/V
= + = + b=1/VM a = tg = kM /VM
Lineweaver-Burk
equation V VM (S) V M (S) VM (S) VM

X=1/S
Y = a X + b -1/k M 0
ANIMATED MODEL OF ENZYME KINETIC
Hanes equation
1 KM (S) KM 1 1
---- = --------- + --------- = ----- ---- + -----
V VM (S) VM (S) VM (S) VM Y=S/V
1 KM 1 1
---- = ----- ---- + ----- x (S)
V VM (S) VM b =KM / VM a = t g =1/ VM
S KM 1 (S)
---- = ----- + -----
V VM VM X=S
-KM 0
Y = b a X

Eddie Hofstee equation

VM (S)
V = --------------
KM + (S)
V KM + V (S) = VM (S) x 1/(S)
Y=V
V KM + V = VM
(S) b = VM a = tg = -KM
V = VM - KM V
(S) X=V/(S)
0
Y = b - a X VM/KM