Professional Documents
Culture Documents
Respiration
3) Oxidative
Phosphorylation (ETC)
Cristae of
mitochondria
Step 1: Glycolysis
“Splitting of sugar”
Breaks down glucose
(C6H12O6) into two
molecules of pyruvic acid
- AKA pyruvate (C3H4O3)
Anaerobic
Occurs in the cytoplasm
NAD picks up H+ and
electrons to form NADH2
Glycolysis Summary
Location: Cytoplasm
Products
Reactants
Products
Reactants
8 NADH (2 from
2 Acetyl Co-A
transition)
2 FADH2
2 ATP
6 CO2 (2 from transition)
Kreb’s Summary
Kreb's Summary 2
Step 3: Electron Transport Chain
(ETC)
Aerobic process
Requires oxygen as the final
electron acceptor
Takes place in the cristae of
the mitochondria
A series of molecules that
excited electrons pass along, to
release energy as ATP
Most of the chain’s components
are proteins, which exist in
multiprotein complexes
Step 3: Electron Transport Chain
(ETC)
Following glycolysis and the citric acid cycle, NADH and
FADH2 account for most of the energy extracted from
food
These two electron carriers donate electrons to the
electron transport chain, which powers ATP synthesis
via oxidative phosphorylation
The carriers alternate reduced and oxidized states as
they accept and donate electrons
Electrons drop in free energy as they go down the chain
They are finally passed to O2 (final electron acceptor),
forming H2O
NADH and FADH2
Dump the electrons and protons they’ve gathered
throughout glycolysis and the citric acid cycle
Again, oxygen is the final electron acceptor
O2 + 2e- + 2H+ H2O
Electrons are passed through a number of proteins
including cytochromes (each with an iron atom) to O2
The chain’s function is to break the large free-energy drop
from food to O2 into smaller steps that release energy in
manageable amounts
ETC uses chemiosmosis to generate large amounts of ATP
Chemiosmosis
Electron transfer in the ETC causes
proteins to pump H+ from the
mitochondrial matrix to the
intermembrane space
H+ then moves back across the
membrane, passing through channels in
ATP synthase (enzyme that acts like an
ion pump)
ATP synthase uses the exergonic flow of
H+ to drive phosphorylation of ADP
This is an example of chemiosmosis,
the use of energy in a H+ gradient to
drive cellular work
The H+ gradient is called the proton-
motive force
ETC Summary
ETC
ETC Summary
Location: Cristae of Mitochondria
Reactants Product
34 ATP
10 NADH
Each NADH makes 3
2 FADH2 Each FADH2 makes 2
Stage ATP
+ 4 Total
Glycolysis + 2 NET (b/c 2 are used in the first step)
CA Cycle +2
ETC +34
_________________
TOTAL + 38
During cellular respiration, most energy flows in this sequence:
Glucose -> NADH -> electron transport chain -> proton-motive
force -> ATP
Review
Role of Macromolecules
Catabolic pathways funnel electrons from many
kinds of organic molecules into cellular respiration
Glycolysis accepts a wide range of carbohydrates
Proteins must be digested to amino acids
Amino groups can feed glycolysis or the citric acid
cycle
Fats are digested to glycerol (used in glycolysis) and
fatty acids (used in generating acetyl CoA)
Fatty acids are broken down by beta oxidation
and yield acetyl CoA
An oxidized gram of fat produces more than twice as
much ATP as an oxidized gram of carbohydrate
Regulation of Cell Respiration
Feedback inhibition is the
most common mechanism
for control
If ATP concentration begins
to drop, respiration speeds
up
When there is plenty of ATP,
respiration slows down
Control of catabolism is
based mainly on regulating
the activity of enzymes at
strategic points in the
catabolic pathway
Review Questions
1. Define cellular respiration and state its importance as a life process.
2. Differentiate between aerobic respiration, anaerobic respiration, and
fermentation.
3. State and explain the chemical equation for cellular respiration.
4. Define oxidation and reduction and explain the idea of redox reactions.
5. Explain the use of NAD+ as a coenzyme.
6. Explain the electron transport chain (ETC).
7. Name the 3 major stages of cell respiration, along with their locations.
8. Explain glycolysis, stating the reactants, products, and major activities.
9. Explain the bridge reaction, stating the reactants, products, and major activities.
10. Explain the Kreb’s cycle, stating the reactants, products, and major activities.
11. Explain glycolysis, stating the reactants, products, and major activities.
12. Explain the ETC, stating the reactants, products, and major activities.
13. Explain the role of oxygen in the ETC.
14. Define chemiosmosis and explain its role in cellular respiration.
15. Differentiate between lactic acid fermentation and alcohol fermentation.
16. Differentiate between oblicate anaerobes and facultative anaerobes.
17. Explain the role of macromolecules in cellular respiration.
18. Explain how cell respiration is regulated.