ELECTRON MICROSCOPE

Presented By:
Satabdi Saikia
B.Sc. 3rd sem.
Roll No.:69
 CONTENTS
 INTRODUCTION
 HISTORY
 TYPES OF ELECTRON MICROSCOPE
 APPLICATIONS
 ADVANTAGES & DISADVANTAGES
 CONCLUSION
 REFERENCES
 INTRODUCTION
 Electron microscope uses a beam of
electrons to create an image of the specimen.
It has much higher magnification and greater
resolving power.
They are large , expensive and pieces of
equipment , generally standing alone in a small ,
specially designed room and requiring trained
personnel to operate them.
 HISTORY

 The physicist
Ernest Ruska
constructed the first
electron microscope
in 1931.
TYPES OF ELECTRON MICROSCOPE
Electron microscopes are of two types:
 Transmission Electron Microscope (TEM)
 Scanning Electron Microscope (SEM)

Fig. :TEM Fig.: SEM

Transmission Electron Microscope (tem) :

Fig.: Formation of image in TEM

SCANNING ELECTRON MICROSCOPE(SEM):

Fig. :Formation of image in SEM

APPLICATIONS OF ELECTRON MICROSCOPE:

 Scientific research: Used to explore the molecular

nature and mechanism of disease , view the 3D
structure of biological tissues and cells , determine
the structure of proteins and observe viruses in a
biological context.
 Industry: Used to assist in developing new
products and throughout the manufacturing process.
 Natural: Used to characterize and analyze organic
materials , which is particularly valuable information
for mining companies.
 Forensic Science: Used to analyze the details of a
relevant specimen.
Advantages of Electron Microscope:
Have higher magnification.
The potential runs the gamut of scientific fields
including biology , medical and forensic sciences ,
metallurgy and nanotechnology.
Also used in many technological and industrial
applications.
Disadvantages of Electron Microscope
They are very costly.
Vibrations and external magnetic fields are very
damaging to it.
Preparation of sample is time consuming and requires
expert training.
Living organisms cannot be studied.
 CONCLUSION

From the above discussion we come to

know that the invention of electron
microscope has been a valuable tool in the
development of scientific theory. It has
contributed greatly to biology , medicine and
material sciences.
 REFERENCES
1. Bajpai,P.K.(2006): Biological Instrumentation and Methodology
(2nd Edn.),S.Chand &Company pvt. Ltd.,Ramnagar,New Delhi,
PP 39-49

2.https://en.m.wikipedia.org/wiki/Electron _Microscope