PROTEIN

ISOLATION OF BEAN PROTEINS

PROTEINS
-most abundant organic molecules of the living system
-Its fundamental basis of structures and function of life
-50% of dry weight of every cell
-It’s a polymer of L a-amino acids
-300 different amino acids occur in nature only 20 as standard amino acids
-21 amino acid added- Selenocysteine

is the basic component of living cells and is made of carbon,hydrogen,oxygen,nitrogen and one
or more chains of amino acids. The three types of proteins are fibrous, globular, and
membrane. Proteins plays countless roles throughout the biological world , from catalyzing
chemical reactions to building the structures of all living things. Despite this wide range of
functions, all proteins are made out of the same twenty one amino acids, but combined in
different ways. The way these twenty amino acids are arranged dictates the folding of the
protein into its unique final shape. Since protein function is based on the ability to recognize
and bind to specific molecules, having the correct shape is critical for proteins to do their jobs
correctly.
ISOLATION OF BEAN PROTEIN
1. Sort and wash 1 cup of proteins.
2. Soak in water for one day.
3. Grind the beans finely with water and blend with more water
using the waring blender.
4. Express the milk through a cheesecloth and discard the insoluble
residue.
5. Add 1M HOAc until precipitation is complete. Allow the mixture
to stand for sometime.
6. Strain through cheesecloth and squeeze the water out as much as
possible.
7. Dry the precipitate by pressing between filter paper.
8. Weigh the dry precipitate.
Reagents and Apparatus
ISOLATION OF PROTEINS

BEAN PROTEIN
Weight of sample
Weight of protein
% protein by weight
Post Laboratory Guide Questions:
1. What is the purpose of soaking the beans in water?
2. What are the predominant proteins present in beans?
3. How are these proteins precipitated by an acid?
What are the protein present the
peanut?
Arachin
a globulin costituting the chief protein of the
peanut.
Conarachin
is much more soluble than arachin. It can not
be precipitated from its salt solution by dilution with
water without the use of enormously large volumes.

The two proteins are reported to have the same

association-dissociation behavior properties in
sucrose as in low ionic strength phosphate buffer.
What are the importance of proteins
and it’s sources?
Function of proteins in the human
body?
Protein test Positive Negative
Biuret -
Ninhydrin +
Xanthoproteic +
Millon’s +
Hopkins-cole -
sakaguchi -
Pb(Ac)2 -
Positive Negative