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  • Calibration of Microscope

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    zee
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    The document describes how to calibrate an eyepiece graticule used to measure objects under a microscope. The eyepiece graticule has arbitrary divisions that need to be calibrated against a stage micrometer with known divisions. To calibrate it, the two scales are overlapped and the number of graticule and micrometer divisions over the same distance are counted. This allows calculating the size of one graticule division in millimeters or micrometers, which can then be used to determine the actual size of any objects measured using the graticule.

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    calibration of microscope

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    The document describes how to calibrate an eyepiece graticule used to measure objects under a microscope. The eyepiece graticule has arbitrary divisions that need to be calibrated against a stage micrometer with known divisions. To calibrate it, the two scales are overlapped and the number of graticule and micrometer divisions over the same distance are counted. This allows calculating the size of one graticule division in millimeters or micrometers, which can then be used to determine the actual size of any objects measured using the graticule.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
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    23 views12 pages

    Calibration of Microscope

    Uploaded by

    zee
    The document describes how to calibrate an eyepiece graticule used to measure objects under a microscope. The eyepiece graticule has arbitrary divisions that need to be calibrated against a stage micrometer with known divisions. To calibrate it, the two scales are overlapped and the number of graticule and micrometer divisions over the same distance are counted. This allows calculating the size of one graticule division in millimeters or micrometers, which can then be used to determine the actual size of any objects measured using the graticule.

    Copyright:

    © All Rights Reserved
    Download as PPT, PDF, TXT or read online from Scribd
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    of 12

    Calibration of Eyepiece Graticules

    How do we measure cells?

    eye piece graticule

    placed in the eyepiece of a microscope

    scale changes with each objective lens


    therefore need to calibrate
    Eye Piece Graticules

    Fitted in the
    eyepiece

    100 divisions

    Arbitrary units

    Needs Calibration
    Stage Micrometer

    10mm

    100 divisions

    Each is 0.1mm
    To Calibrate

    Superimpose the two scales


    Match up two points
    Count the number of epg divisions = x
    Count the number of micrometer divisions = y
    One micrometer division is 0.1mm

    Total width = y x 0.1mm

    If there are x epg divisions


    So x epg divisions = y x 0.1mm

    then ONE epg division = (y x 0.1) mm


    X

    You may need to convert to


    micrometers
    If you have an object on this power you can now
    find the Actual Size of the image

    Count the number of epg divisions


    Multiply by the calibrated width of one division
    Size of drawing (mm)
    Magnifcation =
    Actual size of object (mm)
    Using an eye piece graticule

    1. eye-piece graticule 2. stage micrometer scale

    100 eye piece graticule measurements:


    0.25mm
    1 eye piece graticule measurement:
    0.25/100 = 0/0025mm (2.5µm)
    Using an eye piece graticule

    3. cheek cell: 20 units

    cheek cell: 20 units x 2.5µm = 50µm

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