Elemental Analysis - Atomic Spectroscopy

A) Introduction
Based on the breakdown of a sample into atoms, followed by the measurement of the
atom’s absorption or emission of light.
i. deals with absorbance fluorescence or emission (luminescence) of atoms or
elemental ions rather then molecules
- atomization: process of converting sample to gaseous atoms or
elementary ions
ii. Provides information on elemental composition of sample or compound
- UV/Vis, IR, Raman gives molecular functional group information, but no
elemental information.
iii. Basic process the same as in UV/Vis, fluorescence etc. for molecules

E1
h
Eo
Absorbance Fluorescence
 iv. Differences for Molecular Spectroscopy
- no vibration levels  much sharper absorbance, fluorescence, emission
bands
- position of bands are well-defined and characteristic of a given element
- qualitative analysis is easy in atomic spectroscopy (not easy in molecular
spectroscopy)
Examples:
carbon

oxygen

nitrogen
B) Energy Level Diagrams
energy level diagram for the outer electrons of an element describes atomic spectroscopy
process.
i. every element has a unique set of atomic orbitals
ii. p, d, f split by spin-orbit coupling
iii. Spin (s) and orbital (l) motion create magnetic fields that perturb each other (couple)
- parallel  higher energy; antiparallel  lower energy

• Similar pattern between atoms but

different spacing

• Spectrum of ion different to atom

• Separations measured in
electronvolts (eV)
1eV = 1.602x10-19 J
= 96.484 kJ mol-1

• As number of electrons increases,

number of levels increases
emission spectra more complex
Li 30 lines
Na Mg+ Cs 645 lines
Cr 2277 lines

Note slight differences in energy due to

magnetic fields caused by spin
C) Desire narrow lines for accurate identification
Broadened by
i. uncertainty principle

Uncertainty principal:

t . E ≥h

t .  ≥ 1

t – minimum time for measurement

 – minimal detectable frequency difference

Peak line-width is defined as width in wavelength at half the signal intensity

 C) Desire narrow lines for accurate identification
Broadened by
ii. Doppler effect

Doppler effect
- emitted or absorbed wavelength changes as a result of
atom movement relative to detector
- wavelength decrease if motion toward receiver
- wavelength increases if motion away from receiver

Usage in measurement of velocity of galaxies, age of universe and big bang theory
C) Desire narrow lines for accurate identification
Broadened by
iii. Pressure broadening

Pressure broadening:
Collisions with atoms/molecules transfers small quantities of vibrational energy
(heat) - ill-defined ground state energy

Effect worse at high pressures:

• For high pressure Xe lamps (>10,000 torr) turns lines into continua!
D) Effect of Temperature on Atomic Spectra
- temperature changes number of atoms in ground and excited states
- need good temperature control

Boltzmann equation

N1 and No – are the number of atoms in excited and ground states

k – Boltzmann constant (1.28x10-23 J/K)
T – temperature
E – energy difference between ground and excited states
P1 and Po – number of states having equal energy at each quantum level

Na atoms at 2500 K, only 0.02 % atoms in first excited state!

Less important in absorption measurements - 99.98 % atoms in ground state!

E) Sample Atomization – expose sample to flame or high-temperature
i. Need to break sample into atoms to observe atomic spectra

ii. Basic steps:

a) nebulization – solution sample, get into fine droplets by spraying thru thin nozzle or
passing over vibrating crystal.
b) desolvation - heat droplets to evaporate off solvent just leaving analyte and other
matrix compounds
c) volatilization – convert solid analyte/matrix particles into gas phase
d) dissociation – break-up molecules in gas phase into atoms.
e) ionization – cause the atoms to become charged
f) excitation – with light, heat, etc. for spectra measurement.
E) Sample Atomization – expose sample to flame or high-temperature
iii. Types of Nebulizers and Atomizers
F) Atomic Absorption Spectroscopy (AAS)
– commonly used for elemental analysis
– expose sample to flame or high-temperature
– characteristics of flame impact use of atomic absorption spectroscopy

Flame AAS:
• simplest atomization of gas/solution/solid
• laminar flow burner - stable "sheet" of flame
• flame atomization best for reproducibility (precision) (<1%)
• relatively insensitive - incomplete volatilization, short time in flame
i. Different mixes and flow rates give different temperature profile in flame
- gives different degrees of excitation of compounds in path of light source
ii. Types of Flame/Flame Structure – selection of correct flame region is important for
optimal performance

a) primary combustion zone – blue inner cone (blue due to emission from C 2, CH &

other radicals)
- not in thermal equilibrium and not used
b) interconal region
- region of highest temperature (rich in free atoms)
- often used in spectroscopy
- can be narrower in some flames (hydrocarbon) tall in others (acetylene)
Temperature varies across flame –
c) outer cone need to focus on correct part of flame
- cooler region
- rich in O2 (due to surrounding air)
- gives metal oxide formation

Primary region
for spectroscopy

Not in thermal equilibrium

and not used for
spectroscopy

Flame profile: depends on type of fuel

and oxidant and mixture ration
Most sensitive part of flame for AAS varies with analyte

Consequences:
- Sensitivity varies with element
- must maximize burner position
- makes multi-element detection difficult
iii. Basic instrument design (Flame atomizer)

Single beam

Double beam
a) atomizer
1) Laminar Flow Burner
- adjust fuel/oxidant mixture for optimum excitation of desired
compounds
- usually 1:1 fuel/oxidant mix but some metals forming oxides use
increase fuel mix
- different mixes give different temperatures.

Laminar – non-turbulent streamline flow

i. sample, oxidant and fuel are mixed

ii. only finest solution droplets reach
burner
iii. most of sample collects in waste
iv. provides quite flame and a long path
length
2) Electrothermal (L’vov or Graphite furnace)
- place sample drop on platform inside tube
- heat tube by applying current, resistance to current creates heat
- heat volatilizes sample, atomizers, etc. inside tube
- pass light through to measure absorbance

Po

P
Place sample
droplet on platform
3) Comparison of atomizers

b) Laminar Flow Burner

advantages:
- good b (5-10 cm)
- good reproducibility

disadvantages:
- not sample efficient (90-99% sample loss before flame)
- small amount of time that sample is in light path (~10 -4 s)
- needs lots of sample
b) Light source
- need light source with a narrow bandwidth for light output
- AA lines are remarkably narrow (0.002 to 0.005 nm)
- separate light source and filter is used for each element

1) problem with using typical UV/Vis continuous light source

- have right , but also lots of others (non-monochromatic light)
- hard to see decrease in signal when atoms absorb in a small bandwidth
- only small decrease in total signal area
- with large amount of elements  bad sensitivity
 2) Solution is to use light source that has line emission in range of interest
- laser – but hard to match with element line of interest
- hollow cathode lamp (HCL) is common choice

Hollow Cathode Lamp Coated with element

to be analyzed

Process: use element to detect element

1. ionizes inert gas to high potential (300V)
Ar  Ar+ + e-
2. Ar+ go to “-” cathode & hit surfaces
3. As Ar+ ions hit cathode, some of deposited element is excited and
dislodged into gas phase (sputtering)
4. excited element relaxes to ground state and emits characteristic radiation
- advantage: sharp lines specific for element of interest
- disadvantage: can be expensive, need to use different lamp for each element tested.
c) Source Modulation (spectral interference due to flame)
- problem with working with flame in AA is that light from flame and light source
both reach detector
- measure small signal from large background
- need to subtract out flames to get only light source signal (P/P o)

i. done by chopping signal:

Flame + P

Flame only
P Flame + P
ii. or modulating P from lamp:

Flame only
time
d) Corrections For Spectral Interferences Due to Matrix
- molecular species may be present in flame
- problem if absorbance spectra overlap since molecular spectrum is much
broader with a greater net absorbance
- need way of subtracting these factors out
Methods for Correction

1) Two-line method
- monitor absorbance at two  close together
 one line from sample one from light source
 second from impurity in HCL cathode, Ne or Ar gas in HCL, etc
- second must not be absorbed by analyte
 absorbed by molecular species, since spectrum much broader
- A &  are ~ constant if two  close
- comparing A1, A2 allows correction for absorbance for molecular species

A1 (atom&molecule) – A2 (molecule) = A (atom)

Problem: Difficult to get useful second with desired characteristics

2) Continuous source method
- alternatively place light from HCL or a continuous source D 2 lamp thru flame
- HCL  absorbance of atoms + molecules
- D2  absorbance of molecules

advantage:
-available in most instruments
-easy to do
disadvantage:
-difficult to perfectly match lamps (can give + or – errors)
3) Zeeman Effect
- placing gaseous atoms in magnetic field causes non-random orientation of
atoms
- not apparent for molecules
- splitting of electronic energy levels occurs (~ 0.01 nm)
- sum of split absorbance lines  original line Background
- only absorb light with same orientation
- can use Zeeman effect to remove background
 
place flame polarized light through
  
sample in magnetic field get
absorbance (atom+molecule) or
Background+Absorbance
absorbance (molecule) depending
on how light is polarized

  
e) Chemical Interference - more common than spectral interference

1) Formation of Compounds of Low Volatility

- Anions + Cations  Salt
Ca2+ +SO42-  CaSO4 (s)
- Decreases the amount of analyte atomized  decreases the absorbance signal
- Avoid by:
 increase temperature of flame (increase atom production)
 add “releasing agents” – other items that bind to interfering ions
eg. For Ca2+ detection add Sr2+
Sr2+ + SO42-  SrSO4 (s)
increases Ca atoms and Ca absorbance
 add “protecting agents” – bind to analyte but are volatile
eg. For Ca2+ detection add EDTA4-
Ca2+ + EDTA4-  CaEDTA2-  Ca atoms

2) Formation of Oxides/Hydroxides
M + O  MO

non-volatile & intense molecular absorbance
M + 2OH M(OH)2

- M is analyte
- Avoid by:
 increase temperature of flame (increase atom production)
 use less oxidant
3) Ionization
M  M+ + e-

- M is analyte
- Avoid by:
 lower temperature
 add ionization suppressor – creates high concentration of e - suppresses
M+ by shifting equilibrium.
G) Atomic Emission Spectroscopy (AES) – similar to AA with flame now being used for
atomization and excitation of the sample for
light production
1) Atomic Processes

heat

Degree of Excitation Depends on Boltzmann Distribution:

N1 and No – are the number of atoms in excited and ground states

k – Boltzmann constant (1.28x10-23 J/K)
T – temperature
E – energy difference between ground and excited states
P1 and Po – number of states having equal energy at each quantum level

Increase Temperature  increase in N1/No (more excited atoms)

I (emission) N1, so signal increases with increase in temperature
 Need good temperature control to get reproducible signal
eg. For Na, temperature difference of 10o 2500  2510
results in a 4% change in N1/No

Temperature Dependence Comparison between AA and AES:

- AA is relatively temperature independent. Need heat only to get

atoms, not atoms in excited state.
- AA looks at ~ 99.98% of atoms
- AES uses only small fraction (0.02%) of excited atoms

2) Comparison of AA and AES Applications

AES - emission from multiple species simultaneously

Comparison of Detection Limit

Flame Emission More Sensitivity About the Flame Absorption
Sensitive Same More Sensitive
Al, Ba, Ca, Eu, Ga, Ho, Cr, Cu, Dy, Er, Gd, Ge, Ag, As, Au, B, Be, Bi,
In, K, La, Li, Lu, Na, Mn, Mo, Nb, Pd, Rh, Cd, Co, Fe, Hg, Ir, Mg,
Nd, Pr,Rb, Re, Ru, Sc, Ta, Ti, V, Y, Zr Ni, Pb, Pt, Sb, Se, Si,
Sm, Sr, Tb, Tl, Tm, W, Sn, Te, Zn
Yb
Some better by AA others better by AES
3) Instrumentation
- Similar to AA, but no need for external light source (HCL) or chopper
look at light from flame
 flame acts as sample cell & light source

Atomization Sources:

Source Temperature (oC)

Flame 1700-3150
Plasma 4,000-6,000
Arc/Spark 4,000-5,000/40,00

Electrothermal usually not used – too slow and not as precise

a) Flame Source:
- used mostly for alkali metals
 easily excited even at low temperatures
- Na, K
- need internal standard (Cs usually) to correct for variations flame

Advantages
- cheap

Disadvantage
- not high enough temperature to extend to many other elements
b) Plasma (inductively coupled plasma - ICP)
- plasma – electrically conducting gaseous mixture (cations & electrons)
- temperature much higher than flame
- possibility of doing multiple element analysis
 40-50 elements in 5 minutes
Advantages
- uniform response
- multi-element analysis, rapid
- precision & accuracy (0.3 – 3%)
- few inter-element interferences
- can use with gas, liquid or solids sample
 Inductively Coupled Plasma (ICP) Emission Spectroscopy
- involves use of high temperature plasma for sample atomization/excitation
- higher fraction of atoms exist in the excited state, giving rise to an increase
in emission signal and allowing more types of atoms to be detected

Ions forced to flow in closed

Temperature Regions
Magnetic field path, Resistance to flow
causes heating
in Plasma Torch

Ar charges
by Tesla coil
(high voltages at high frequency)