Introgression of Vigna mungo

Genome into Vigna radiata

M.NARAYANAN
2019508202
GPB -512
Introduction
 The present investigation was carried out at National Pulses
Research Centre, Vamban to assess the introgression level of
blackgram (Vigna mungo cv. Mash 114) genome into
greengram (Vigna radiata cv. VBN(Gg)2) genome background
through interspecific hybridization.
 Superior progenies with high yield potential and MYMV
disease resistance were selected in F4 generation of cross
VBN(Gg)2 and Mash 114 and evaluated in F5 generation.
 Based on per se and yield performance, six superior F5
progenies were selected and subjected to background analysis.
 A set of 84 SSR primers were surveyed and 33 SSRs were
found to be polymorphic between parents
Black gram(Vigna mungo)
 Low yield
 Susceptibility to pests and diseases, among which mungbean
yellow mosaic virus (MYMV)
 Erect growth habit
 Fast-growing annual, 
 The pods are narrow, cylindrical and up to six cm long.
 The plant grows 30–100 cm with large hairy leaves .
Greengram(Vigna radiata)
 Greengram possess early maturity,
 Erect growth habit and
 Long pods with a greater number of seeds/pods
 Non-shattering pods
 Synchronous maturity,
 More clusters/plant
 Comparatively more durable resistance to yellow mosaic virus
disease.
MATERIALS AND METHODS
 Crosses were made between greengram [Vigna radiata cv.
VBN (Gg) 2]
 Blackgram (Vigna mungo cv. Mash 114) and forwarded up to
F4 generation
 Each progeny was evaluated along with highly susceptible
variety, CO5 of blackgram [Vigna mungo (L.) Hepper]
 As infector 2 row to raise MYMV disease incidence.
Hybridization (Interspecific)
 In the present investigation, F4 population obtained from the
cross between Vigna radiata cv VBN (Gg) 2 and Vigna mungo
cv Mash114 were evaluated.
 The female parent, Vigna radiata cv VBN(Gg)2 has lobed leaf
shape and highly susceptible to MYMV.
 Whereas the male parent Vigna mungo cv Mash114 has ovate
leaf shape and highly resistant to MYMV disease.
 A total of 23 single plants with MYMV resistance were
selected in F4 generation and evaluated in F5 generation.
 In F5 generation the variation in plant morphology and
MYMV resistance proves the successful transfer of blackgram
(Vigna mungo cv. Mash 114) genome into the interspecific
derivatives.
 Among the 23 F5 progenies, six progenies viz. 3-1-11, 3-7-6,
3-7-8, 3-10-19, 3-12-13 and 3-13-3 recorded significant
superior performances for seed yield per plant than check
variety VBN(Gg)2
 Among these superior progenies, four interspecific progenies
viz. 3-1-11, 3-7-6, 3-10-19 and 312-13 had ovate leaf shape.
 which showed the introgression of Vigna mungo cv. Mash 114
into Vigna radiata cv. VBN (Gg) 2.
 Wider variation for morphology traits viz., seed colour,
hypocotyl pigmentation and branching pattern was observed
among progenies
 The background analysis revealed that 10.9 to 34.9 per cent
of Vigna mungo cv. Mash 114 genome has been successfully
introgressed in progenies.
 Among the progenies, the proportion of homozygote segments
ranged from 5.4 to 23.8 per cent and heterozygotes ranged
from 7.2 to 22.2 per cent for blackgram genome
 The progeny, 310-19 recorded maximum (34.9%) of
blackgram genome and it showed resistance towards MYMV
disease incidence
 The variation in the allelic pattern viz., homozygotes,
heterozygotes and recombinant segments among the
interspecific derivatives even in the F5 generation might be
due to non-attainment of homozygosity in early generation.
 The superior performances of the progenies
◦ for number of branches per plant,
◦ pods per plant,
◦ seed yield per plant
◦ MYMV disease resistance than the female parent
VBN(Gg)2 might be due to the influence of
introgressed blackgram genome.
 Further selection among the selected progenies may

result in a high yielding greengram variety with

MYMV disease resistance
CONCLUSION
 Our results demonstrate that wide hybridization is an
important tool in crop improvement.
 In the present investigation, successful introgression of Vigna
mungo genome into Vigna radiata genome has been achieved.
 The morphological variation observed for the traits such as
leaf shape, seed color, hypocotyl pigmentation and branching
pattern emphasize that greengram has been introgressed with
blackgram genome
 Background analysis using SSR markers confirmed the
successful introgression of blackgram genome into greengram
genome
References
 https://en.wikipedia.org/wiki/Vigna_mungo
 Introgression of Vigna mungo Genome into Vigna radiata
Towards the Broadening of Genetic Base for MYMV
Resistance and Yield Attributes S. Ragul, N. Manivannan*
and A. Mahalingam
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