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6 stages involved in Genetic Modified Organism

1. Identify the gene of interest.

2. Insertion of genes by microinjection into new fertilized egg.

3. Embryo transferred to female to produce offspring.

4. Verification of transformation and characteristics of the inserted DBA fragments

5. Testing organism performance

6. Safety assessments.
Insertion and transfer of genes
Detection of Transgene
• Southern blot is used.
Performance of Transgenic Tuna
• Compare the growth rate
• Compare the phenotype
Safety Assessments
• Risk:
• Food safety
• Environment risk

• Precaution:
• Transgenic sterilization
• Containment methods

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