Overview of the QuantStudio™ 3 Real-Time

PCR System

The world leader in serving science

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 QuantStudio™ 3 Real-Time PCR Systems: The Basics

• Touchscreen
• WiFi connectivity, enabling remote monitoring
• Low maintenance
• Factory calibrated for Applied Biosystems™ reagents
• Connectible to Browser Software (Cloud), enabling PC/MAC
compatibility

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QuantStudio™ 3 Real-Time PCR Systems: The Basics

Touchscreen (stand-
alone capabilities,
PIN-protected user
accounts, and dye
calibration/RNaseP
functionality) USB ports

WiFi adapter
USB port for
port (optional use)
template upload
and data download Ethernet port :
RJ45
(10/100Mbps)
Motorized
block drawer
(controlled by Fuse cover
touchscreen)

Power port: 100/240

VAC
RS232 port (Service
only)

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QuantStudio™ 3 Real-Time PCR System: The Basics
• VeriFlexTM Blocks
• 3 programmable zones
• Independent temperature control in each zone (more precise than gradient)
• Can program at will, including multiple zones with same temp
• Great for optimization and also running multiple assays at the same time

60°C 61°C 62°C

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Veriflex™ Blocks: Temperature Optimization
60oC 63oC 65oC

• Power SYBR™ Green Master Mix, with

primers designed using Primer Express™
Software
• Secondary peak appears at 90°C when
annealing temperature goes above 63°C
60oC 63oC 65oC

TCF3 Assay
4 replicates for each temperature
Data courtesy of Verification and Validation

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 Multiplexing Capabilities

• OptiFlex™ System
with Bright White LED
• Four color locked
filter system
• Factory calibrated

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 Stand-alone, Desktop, or Online

WiFi

Connected Laptop
with QuantStudio LAN
Design and
Analysis desktop
software

Note: You can start an

experiment run only Connect to the Design
from the instrument and Analysis Cloud
touchscreen or from software using any
the Desktop Software device with a compatible
USB
web browser
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 Stay connected to your data, anytime, anywhere
For real-time PCR users who need an affordable, easy to use real-time PCR system without
compromising on performance and quality, the QuantStudio 3 system enables users to
collaborate, access and share data easily within the Thermo Fisher Cloud ecosystem.

Accessibility
• Access experiment runs from any location anytime with remote
monitoring using the Thermo Fisher Cloud
• Wi-Fi enabled connectivity
• Utilize portable devices to quickly analyze data when you need it

Interactivity
• Interactive touch screen
• Run and edit directly from touch screen
• Easy and intuitive interface

Collaboration
• Quickly share data sets and protocols online
• Send large files securely around campus or around the world
• Integrate and analyze multiple data sets and data types into one
project

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 Global Ecosystems

Monitor Access
global data
outbreaks anywhere

Upload Centralize
new SOPs data with
and Share
with labs in localized
protocols projects
remote
areas

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How the Cloud is utilized with QuantStudio™ 3
• Access/share data
• Analysis Apps
• Browser Based
• Storage and Backup

• Load run/plate • Monitor Run

• Create Studies • Perform Run • View Results
• Analyze Data with Apps • Monitor Run
• Setup run/plate • View Results
• Monitor Run • Calibration
• View Results

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 QuantStudio™ 3 Touch Screen

Open/Close
door

Help

Sign In or
Create a
Local User Settings:
Account - Calibration
- Runs
- Logs
Connection - Ship Prep
type

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 Instrument Local User Accounts

Create local pin- Connect to online

protected account Thermo Fisher
on instrument Cloud account

Optional but highly recommended for full capabilities

• Create individual accounts for multiple users

• PIN-protected accounts help keep protocols and data safe and stop
“accidental” run interruptions
• Instrument users can be designated as “Admin” or “Standard”
users
• First user defaults to “Admin” status but can create other Admins, as
needed

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 Create Local User Account and Link to Cloud

Enter User Name Set PIN

(and remember it!)

Why link to the Cloud?

The Cloud enables you to download run files from the
cloud and automatically upload them when complete

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 Admin and Standard User Accounts

Note: the first user with “Admin” status to connect to the

Cloud will be the Cloud Admin for that linked instrument

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 Use touchscreen to edit reagent info, destination,
and plate setup

2D reagent
barcodes
supported for
Applied
Biosystems
reagents!

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 Access run files from multiple locations

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 Edit run protocol

Full method editing capabilities on the touch screen,

including VeriFlex, Pause, and Melt

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 Monitor Progress During the Run

Time Remaining Thermal Protocol Status Live Amplification Curves

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 Review amp plots in real time

Review well details and

select amplification plots to
view by Target, Sample,
Task, or Master Mix

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 New Feature: Run Pause

Program a Pause into the run:

define which step and at what
temperature to pause

Or pause a run on the fly

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 Options to Upload Data

1. Cloud = Data saved to user’s online account

2. USB = Data saved to attached USB drive
3. Desktop = Data automatically saves back to desktop if run
started from desktop

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 Recommended Calibration Schedule

Calibration type Frequency

ROI/Uniformity

Background Every two years

Dye
• After installing or moving the instrument
RNase P instrument
• As needed thereafter to confirm
verification run
instrument performance

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 QuantStudio™ Design and Analysis
Software

The world leader in serving science

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 QuantStudio™ Design and Analysis Software

• The QuantStudio Design and Analysis Software supports a

variety of analysis methods, including:
• Absolute Quantitation
• Standard Curve
• Relative Quantitation
• Relative Standard Curve
• Comparative CT (∆∆ CT)
• Multiplate ∆∆ CT Studies
• Presence/absence (Plus/Minus) assays with an internal positive
control
• Melt curve analysis
• Genotyping (including real-time amplification)
• Multiplate GEx analysis available online on the
QuantStudio Design and Analysis Cloud Software

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 QuantStudio™ Design and Analysis Desktop and
Cloud software

WiFi

Connected Laptop
with QuantStudio LAN
Design and
Analysis desktop
software

Connect to the Design

and Analysis Cloud
software using any
USB device with a compatible
web browser
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 QuantStudio™ Design and Analysis Cloud
software: Instrument Admin? (Optional)

Manage connected
instruments

Note: If a Cloud admin is not setup at

install, any lab user who connects the Allow others to
instrument online will become the admin! connect

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 Connected Instrument

1. Monitor run progress

2. Review calibration status
3. Check instrument
statistics
a. Runs by user
b. Runs by type

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 Start Run or Open a Run File

“Your Apps”

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 Start Run or Open a Run File

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 Remember to Save!

• When a run file is created, it will exist as an .edt file until it is run
• The .edt file will remain, even after the .eds file is generated

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 Applied Biosystems™ 2D Barcodes

Use a 2D barcode scanner to

track reagent details:
• Part Number
• Lot Number
• Expiration Date

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 Settings
• Veriflex™ blocks
• AutoDelta

Programmable Pause

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 Import and Export

Optional passive reference

Add columns

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 • Import & Export
• Save plate image
• Flip plate setup
Select well and type
sample names

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 Start Run from touchscreen or desktop
(not Cloud)

Monitor Run from “Instruments”

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 Amplification Curve only, e.g. no Genotypes

Scroll from target to target

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Export to local computer:
Text, csv, Excel™, and RDML
(xml)

Report saved online,

option to download
as PDF

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 Desktop – For those who can not be online

• Similar look and feel as online software

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 Applied Biosystems™ 2D Barcodes

Use a 2D barcode scanner to

track reagent details:
• Part Number
• Lot Number
• Expiration Date

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 Programmable Pause
Advanced Settings
• Veriflex™
• AutoDelta

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 Import and Save to
Library

Optional passive reference

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 Select well and type
sample names

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 Start Run from touchscreen or desktop

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 Toggle
between plate
view and well
details

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 Appendix

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 QuantStudio™ 3 Enables 1.5-Fold Discrimination

Amplification plots for 1.5-fold

dilutions of KAZ plasmid amplified
with PE2 TaqMan™ assay under
standard Fast run conditions using
the TaqMan Fast Advanced Master
Mix.

Quantity CT Std Dev

1000 27.9 0.063
1500 27.45 0.059
3000 26.40 0.060
4500 25.80 0.047
6667 25.20 0.049
10000 24.50 0.041

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 © 2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the
property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under
permission and license. Excel is a trademark of Microsoft Corporation.

Note: For Research Use Only. Not for use in diagnostic procedures.

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