Hemoglobin

& Myoglobin
Hemoglobin
Outline

• Defi n i t i o n

• Di ff ere nt Typ es

• S t ru ct u res

• F un ct i o ns
 Definition

• Ir on -co nt ai n i n g pr ot ei n

• Loca t io n : i n t h e R BC o f v ert eb rat es

• Di ff ere nt S t at u s

Status Color A.K.A

Oxygenated Bright Red oxyhemoglobin
Reduced Purplish blue Deoxyhemoglobin
Different Types of Hemoglobin

Type of Hb Levels Indication

Hemoglobin A1 12.1~16.3g/dl, 90% Anemia or blood loss
Hemoglobin A2 1.5%~3.5% Thalassemia
Hemoglobin F 50~90% in neonates Thalassemia
0%~1% in adults

Hemoglobin S Presence is abnormal Sickle cell disease

Hemoglobin Plasma 5 mg/dl Hemolytic anemia
Structures

• Quat e rnar y s t ru ct ur e

• Al ph a h el i ces

• Hyd ro g en b o nd s

• P or ph y ri n

• Ir on i o n
Functions

• t ran s po rt at i o n o f o xy g en an d o t her g as

• mo du l at i o n of ery t h ro cyt e met ab ol i s m

• hem e o xi d at i on an d ery t h ro cyt es sen es cence

• res i s t ance t o m al ar i a

• mo l ecul ar h eat t r an s du cer

• enzy ma t ic act i v i ty
Myoglobin
Outline

• Defi n i t i o n

• S t ru ct u res

• F un ct i o ns

• Co mp ari s o n
Definition

• Loca t io n : car di ac m u scl e and s k el et al mu s cl e t i s s ue of ve rt eb rae i n g en eral

• An i ro n – an d o xy gen – b i nd i n g pro t ei n
Function

• 1. s up p ly o xy g en t o m u scl e

• 2. ox y gen r es e rvo i r i n mu s cl e

• 3. bu ffer o f i n t racel l u l ar ox y gen co ncen t r at i on s

• 4. faci l i t at es o x yg en di ff us i o n

• 5. des at u rat es at t he on s et o f m us cl e ac t iv i t y
Function

• 1. bel o n gs t o t h e g l ob i n s up er fami l y o f p ro t ei n s

• 2. con t ai n s 15 3 ami n o aci ds

• 3. con t ai n 8 al ph a h el i ces

• 4. con t ai n s a p or ph yr i n ri n g wi t h an i ro n at i t s cent e r
Comparison
Thank you for
your attention