Preparation And Evaluation Of Anti

Acne Facial Sheet Mask

Pharmaceutical Product Development
K.B. INSTITUTE OF PHARMCEUTICAL
EDUCATION AND RESEARCH
Project Work By Advisor

Rutanshi Patel Dr. Anita Lalwani

Rutva Patel Professor Department Of
Rutul Solanki Pharmaceutics
Rutvik Chabadiya
3 What Is Sheet Mask?
Sheet mask is a serum-soaked cloth sheet that has holes for your eyes,
nose, and mouth. A sheet mask is like a pack of serum. Sheet masks are
infused with hydrating and active ingredients, which will allow your
skin to absorb moisture and actives better. The sheet prevents the water
and the serum from evaporating into the air, making them more
effective.

presentation title 20XX

4

presentation title 20XX

5 AIM
To prepare anti-acne facial sheet mask containing salicylic acid as an
active ingredient.

presentation title 20XX

 PREPARATION OF ANTI–ACNE
6
SERUM
presentation title 20XX
 Preparation Of Anti-acne Serum Sample
7 Ingredient 1st Trial (Qty) 2nd Trial (Qty) 3rd Trial (Qty) 4th Trial (Qty)

Water 30ml 35ml 35ml 35ml

Glycerin 2 ml - - -
EDTA 0.2 gm 0.2 gm 0.2 gm 0.2 gm
PEG 400 2 ml 2 ml 2 ml 2 ml
Carbopol 0.5 gm 0.2 gm 0.2 gm 0.2 gm
Guar Gum 0.2 gm 0.2 gm 0.2 gm 0.2 gm
Propylene - 2 ml 2 ml 2 ml
Glycol
Triethanol - 2 drops 2 drops 2 drops
Amine
Sodium - 0.1 gm 0.1 gm 0.1 gm
Benzoate
Coconut Oil - 3 drops - 3 drops
Result Lumps Precipitation Do not give Perfect
presentation title
formation oily effect consistency
20XX
 Formulation of different batches based on active ingredients
No Ingredients Batch 1 Batch 2 Batch 3 Batch 4 Role of Ingridient
8
1. Water 35ml 35ml 35ml 35ml vehicle
2. Carbopol 0.2gm 0.2gm 0.2gm O.2gm Binding Agent
3. EDTA 0.2gm 0.2gm 0.2gm 0.2gm Chealator, Preservative

4. Guar Gum 0.2gm 0.2gm 0.2gm 0.2gm Thickener

5. PEG 400 2ml 2ml 2ml 2ml Emulsifier,Preservative

6. Propylene Glycol 2ml 2ml 2ml 2ml Humectant, Preservative

7. Triethanol Amine 0.2ml 0.2ml 0.2ml 0.2ml solublizer

8. Sodium Benzoate 0.1gm 0.1gm 0.1gm 0.1gm Preservative

9. Coconut Oil 0.2gm 0.2gm 0.2gm O.2gm Anti-bactarial, Balance pH

10. Tea tree oil - 0.2ml 0.2ml - Anti-bactarial

11. Salicylic acid - - 0.4gm 0.4gm Anti-bactarial

12. Jasmine Oil q.s q.s q.s q.s Fragrance

presentation title 20XX

9 Procedure
 Take weighed amount of water in a beaker.
 Add Carbopol , EDTA , Guar gum, PEG 400, sodium benzoate and
propylene glycol of weighed quantity in the beaker.
 Mix the ingredients on the magnetic stirrer at high shear rate.
 Add active ingredient and stirr again.
 Add coconut oil and mix it till the mixture becomes homogenous.
 Add Triethanol amine in this mixture.
 Add required quantity of the fragrance in this mixture.

presentation title 20XX

10
Design of Experiment

presentation title 20XX

 Factorial Design
No. Ingredients Batch Batch Batch Batch Batch Batch Batch Batch Batch
11 A1 A2 A3 B1 B2 B3 C1 C2 C3
1. Water 87.5ml 87.5ml 87.5ml 87.5ml 87.5ml 87.5ml 87.5ml 87.5ml 87.5ml

2. Carbopol 0.5gm 0.5gm 0.5gm 0.5gm 0.5gm 0.5gm 0.5gm 0.5gm 0.5gm

3. EDTA 0.5gm 0.5gm 0.5gm 0.5gm 0.5gm 0.5gm 0.5gm 0.5gm 0.5gm

4. Guar Gum 0.2gm 0.2gm 0.2gm 0.4gm 0.4gm 0.4gm 0.6gm 0.6gm 0.6gm

5. PEG 400 5 ml 5 ml 5 ml 5 ml 5 ml 5 ml 5 ml 5 ml 5 ml
6. Propylene 5 ml 5 ml 5 ml 5 ml 5 ml 5 ml 5 ml 5 ml 5 ml
Glycol
7. Triethanol 0.2 ml 0.2 ml 0.2 ml 0.2 ml 0.2 ml 0.2 ml 0.2 ml 0.2 ml 0.2 ml
Amine
8. Sodium 0.25gm 0.25gm 0.25gm 0.25gm 0.25gm 0.25gm 0.25gm 0.25gm 0.25gm
Benzoate

9. Coconut Oil 0.5 ml 0.5 ml 0.5 ml 0.5 ml 0.5 ml 0.5 ml 0.5 ml 0.5 ml 0.5 ml

10. Tea Tree Oil 0.5 ml 0.5 ml 0.5 ml 0.5 ml 0.5 ml 0.5 ml 0.5 ml 0.5 ml 0.5 ml

11. Salicylic 0.5gm 1 gm 1.5gm 0.5gm 1 gm 1.5gm 0.5gm 1 gm 1.5gm

Acid
presentation title 20XX
12

presentation title 20XX

 Evaluation Of Anti-acne
13
serum sample

presentation title 20XX

14
Specific Gravity
Equipment: Weighing balance, Pycnometer (Gravity Bottle), Serum Sample, Distilled Water
Procedure:

 Weigh the empty Gravity Bottle and record it.

 Fill the gravity bottle with distilled water to the calibration mark, weigh and record it.

 Empty the gravity bottle.

 Again, fill the gravity bottle with the serum sample to the calibration mark, weigh and record it.

 Calculate the mass and specific gravity by the recorded measures.

Equation:
Specific Gravity of serum sample =

presentation title 20XX

 Viscosity
15
Equipment: Oswald viscometer, stand, measuring cylinder, beaker, serum sample

Procedure:
 Fill the oswald viscometer with distilled water through arm 1 till it reaches to the mark 2 as shown in figure. Now pull
the water to the mark 4 by sucking or blowing.

 The upper meniscus of the water should be aligned with the mark 4.

 Start the stop watch and measure the time, measurement terminates when the meniscus is completely aligned with
the mark 3 and stop the stop watch.

 Measure the time taken by the water to flow through the mark 3 to 4.

 Repeat the procedure with the serum sample and measure the time.

 Now calculate the viscosity by suitable formula.

Equation:
Ƞ2 = * Ƞ1
presentation title 20XX
 Determination Of Drug Content
16
Procedure:
 Preparation of the calibration curve:
 Weigh accurately 100 mg of salicylic acid and transfer it into 100 ml volumetric flask.

 Makeup the volume up to the mark with distilled water.

 From this solution take 10 ml, transfer it into 100 ml of volumetric flask and dilute it up to mark with distilled
water to make 100 microgram/ml.
 From this working standard solution pipette out 1ml, 3ml, 5ml, 7ml, 9ml of solution in 10 ml volumetric flask

and add 1 ml of 1% ferric chloride (FeCl3) solution and make up to the volume 10 ml with distilled water.

 Find absorbance of every sample at 540 nm.

 Prepare the calibration curve of Absorbance vs Concentration.

 Prepare the serum sample with different concentration, find the absorbance of these sample and determine
the concentration of samples.
presentation title 20XX
17

No Concentration Absorbance
(microgram) Chart Title
0.8
1 10 0.090 0.7 f(x) = 0.00824493150684932 x + 0.00879452054794522
R² = 0.99934498322546
0.6
2 30 0.261
0.5

0.4
3 50 0.416
0.3

4 70 0.594 0.2

0.1

5 90 0.745 0
0 10 20 30 40 50 60 70 80 90 100

Series1 Linear (Series1)

presentation title 20XX

18 Absorbance Of Serum Sample:

No Sample absorbance Concentration

(gm/ml)

1 A1 0.422 0.51
2 A2 0.431 1.03
3 A3 0.263 1.55
4 B1 0.426 0.51
5 B2 0.412 0.99
6 B3 0.249 1.47
7 C1 0.409 0.51
8 C2 0.424 1.01
9 C3 0.272 1.65

presentation title 20XX

19 Anti-micobial Activity

 Organism: Propionibacterium

 Medium: Nutrient agar

 Equipment: Test tubes, Beaker, Petri dish, loop

 Procedure:
 Equally cut filter paper and anti-acne mask sheet was used
 Filter paper was dipped in the prepared solution for half an hour
 Nutrient agar plate was divided into two equal sections
 Nutrient broth culture of Propionibacterium acne was streaked on the surface of agar .
 Filter paper disk and mask sheet were placed gently on each section respectively.
 These streaked plates were put for incubation for 24hrs
20XX
presentation title
 Zone of Inhibition :
Sample A1 Sample A2 Sample A3
20

Sample B1 Sample B2 Sample B3

Sample C1 Sample C2 Sample C3

presentation title 20XX

21
pH of samples
pH of the anti-acne serum should be measured for every sample.
Thus, the pH of the different anti-acne serum samples are measured on the pH meter.
These are the results of the different sample.

SAMPLE PH

A1 7.6
A2 6.34
A3 5.55
B1 6.19
B2 6.33
B3 6.4
C1 6.46
C2 6.25
C3 5.36
presentation title 20XX
22 Amount Of liquid Absorbed:

 Cut 9 dry fabrics into equal size of 2×2 cm.

 Weigh each dry fabrics on weighing machine.

 Dip these fabrics into different anti-acne serum samples.

 Allow these fabrics to remove excess liquid on sieve or 10 minutes.

 Now weigh these fabrics and measure the amount of liquid.

presentation title 20XX

23 Result of Factorial Design:
Batch Viscosity Density Drug Zone of pH Amount of liquid
(Cp) (gm/cm3) Content inhibition absorbed
(gm/ml)

A1 5.76 1.02 0.51 No 6.7 0.305

A2 3.63 1.013 1.03 No 6.34 0.297

A3 3.81 1.017 1.55 3 5.55 0.264

B1 12.67 1.007 0.51 No 6.19 0.312

B2 7.59 1.007 0.99 2.7 6.33 0.370

B3 6.28 1.013 1.47 2.45 6.4 0.365

C1 33.85 1.008 0.51 2.6 6.46 0.418

C2 24.5 1.02 1.01 No 6.25 0.428

C3 29.2 1.009 1.65 2.6 5.36 0.400

presentation title 20XX

 Response 1: Viscosity
24  Final Equation in Terms of Coded Factors
Factor Coding: Actual

m Viscosity
Viscosity = Viscosity

3.63
Design Points

33.85
1

X1 = A

+6.61 X2 = B

dffdfg 0.5

+12.39 A
-2.16 B

B: B
0
20
10

-0.6750 AB
+7.=94 A² -0.5

30

+3.35 B² -1

-1 -0.5 0 0.5 1

A: A

Here,
A = Guar gum
B=
Salicylic Acid
According to this equation, ratio A is a significant model. It means viscosity is directly proportional
to the guar gum. Based on this graph this model is quadratic.
presentation title 20XX
 Response 2 : pH
 Final Equation in Terms of Coded Factors
25 Factor Coding: Actual

pH 1
pH
Design Points

5.36 7.6
X1 = A
X2 = B

pH = 0.5
6

+6.28

B: B
0

-0.2367 A 6.5
-0.5

-0.4900 B
-1

-1 -0.5 0 0.5 1

A: A

According to this equation, ratio B is a significant model. It

means Ph is directly proportional to the salicylic acid. Based on
this graph, this model is linear.

presentation title 20XX

 Response 3: Water
26  Final Equation in Terms of Coded Factors
Factor Coding: Actual
Water
Water 1
Design Points

0.264 0.428
X1 = A
X2 = B

Water = 0.5

+0.3510
0.3 0.4

B: B
0.32 0.34 0.36 0.38
0

+0.0633 A
-0.5

-0.0010 B

-1

-1 -0.5 0 0.5 1

A: A

According to this equation, ratio A is a significant model. It means water

is directly proportional to the guar gum. Based on this graph, this model
is linear.
27 Optimized sample among all:
 The parameters for choosing the most desirable and optimized
batch are higher viscosity, More amount of liquid retention and
lower pH. Based on the above parameters sample C1 can be
considered as the desirable sample to be used in Anti-acne face
sheet mask.

presentation title 20XX

 28

Sample C1 Market Product

Anti-Microbial Activity of Sample C1 and Market Product

Comparison with Market Product: Stability:

Parameter C1 C1
Parameter C1 Market
(initial) (after 45 days)
product

pH 6.46 6.32 pH 6.46 6.53

Liquid 0.418(g 0.411(gm) Liquid 0.418(gm) 0.395(gm)
m)
Micro 2.6(cm) No
Micro No No

presentation title 20XX

29 CONCLUSION
 In this study, many different samples were prepared for checking with main active
ingredient- Salicylic acid which act as an anti-bacterial, anti- inflammatory. during the
formulation process the quality, the appearance and stability of many samples were
affected by exposure of light, density, temperature, PH, viscosity, incompatibility of
active ingredient. Out of all sample, one sample found to be optimal. The parameters
for choosing the most desirable and optimized batch are higher viscosity, more
amount of liquid retention and lower PH. Based on the above parameters sample C1
can be considered as the desirable sample to be used in Anti-Acne face sheet mask.
A great achievement is that the stability, texture, appearance, viscosity, density of
these selected product was maintained for 40 days (one and half month). A more
realistic comparison could be made between our product and marketed product for
acne-prone skin. The subject base evaluation results give us the conclusion that our
product is equipotential to that of marketed product.

20XX
 Formulation of Pilot Plan Scale up
30
No. Ingredients Batch
C1
1. Water 87.5ml
2. Carbopol 0.5gm
3. EDTA 0.5gm
4 Guar gum 0.6gm
5. PEG 400 5 ml
6. Propylene Glycol 5 ml
7. Triethanol Amine 0.2 ml
8. Sodium Benzoate 0.25gm

9. Coconut Oil 0.5 ml

10. Tea Tree Oil 0.5 ml
11. Salicylic Acid 0.5gm
presentation title 20XX
Thank you