Chem 2: Analytical Chemistry

UNIT 1
Introduction to Analytical
Chemistry

Chem 2 Cluster CLASE: Chemistry and Physics Department

 Augustinian Prayer Before the Class
Leader: When we live in unity…
All: How good and pleasant it is.
Leader: Pray for us, Holy Father Augustine
All: That we may dwell together in peace
Leader: Let us pray
All: God, our Father, your Son promised to be present in
the midst of all who come together in His name. Help us to
recognize His presence among us and experience in our
hearts the abundance of Your grace. Your mercy and peace,
in truth and in love. We ask this through Christ, our Lord.
Amen.

Chem 2 Cluster Our Father… CLASE: Chemistry and Physics Department

 Analytical Chemistry

is a measurement in science consisting of a set of

powerful ideas and methods that are useful in all
fields of science, engineering, and medicine.

Two Major Divisions:

1. Qualitative Analytical Chemistry
“chemical identity/ies in the sample”

2. Quantitative Analytical Chemistry

“relative amounts of ….”

Chem 2 Cluster CLASE: Chemistry and Physics Department

 Chemanalysis
Qualitative 1a Course
Qualitativeanalysis is Outline
iswhat.
what.
Quantitative
Quantitativeanalysis
analysisisishow
how much.
much.

©Gary Christian, Analytical Chemistry, 6th Ed. (Wiley)

Chem 2 Cluster CLASE: Chemistry and Physics Department
Chem 2 Cluster CLASE: Chemistry and Physics Department
 Forum Discussion

• Cite specific examples of relating/applying analytical chemistry to

other branches of chemistry and other sciences

Chem 2 Cluster CLASE: Chemistry and Physics Department

 .
Chem 2 Cluster CLASE: Chemistry and Physics Department
 An
Ananalysis
analysisinvolves
involvesseveral
several
steps and operations which
steps and operations which
depend
dependon:
on: Chem 1a Course Outline
•the
•theparticular
particularproblem
problem
••your expertise
your expertise
••the apparatus or
the apparatus or
equipment
equipmentavailable.
available.
The
Theanalyst
analystshould
shouldbe
be
involved in every step.
involved in every step.

©Gary Christian,
Analytical Chemistry,
6th Ed. (Wiley)

Chem 2 Cluster CLASE: Chemistry and Physics Department

Fig. 1.1. Steps in an analysis
 Different
Different methods
methods provide
provide aarange
range of
of precision,
precision,
sensitivity, Chem 1a Course Outline
sensitivity, selectivity,
selectivity, and
andspeed
speedcapabilities.
capabilities.

©Gary Christian,
Analytical Chemistry,
6th Ed. (Wiley)

Chem 2 Cluster CLASE: Chemistry and Physics Department

 Calibration
Calibrationcurves
curvesare
areprepared
preparedfrom
fromstandards.
standards.
Chem 1a Course Outline
Unknown
Unknownconcentrations
concentrationsare
arethen
thendetermined
determined
from
fromthe
thesignal
signalintensity
intensityof
ofthe
thesample.
sample.

Figure 1.2 Calibration curves for the measurement of proteins

using matrix-assisted laser desorption ionization (MALDI) – mass
spectrometry and anionic liquid matrix. (Courtesy of Prof. Michael
Gross, Washington University in St. Louis.)
Chem 2 Cluster CLASE: Chemistry and Physics Department ©Gary Christian, Analytical Chemistry,
6th Ed. (Wiley)
 Example 1: Chemical Analysis

Chem 2 Cluster CLASE: Chemistry and Physics Department

Chem 2 Cluster CLASE: Chemistry and Physics Department
Chem 2 Cluster CLASE: Chemistry and Physics Department
Chem 2 Cluster CLASE: Chemistry and Physics Department
Chem 2 Cluster CLASE: Chemistry and Physics Department
Chem 2 Cluster CLASE: Chemistry and Physics Department
 HPLC
-instrument used for the separation and quantification of compounds

Chem 2 Cluster CLASE: Chemistry and Physics Department

 Example 2: Chemical Analysis
Quantification
of Caffeine in
Selected Energy
Drinks
-Carisma, M., Loking
D., Saludes, J.
(Faculty Research)

Chem 2 Cluster General flow ofandpreparation

CLASE: Chemistry Physics Departmentand analysis of samples.
 Augustinian Prayer After the Class
Leader: Our help is in the name of the Lord..
All: Who made heaven and earth.
Leader: Let us pray
All: God, the desire of every human heart, You,
moved St. Augustine to seek restlessly for truth and
peace. Touch our hearts with His burning desire for
wisdom, for the Word made flesh. We ask this
through Christ, our Lord. Amen.

Glory be to the Father…

Chem 2 Cluster CLASE: Chemistry and Physics Department