BIOLOGY FORM 4, PAPER 3

AUGUST TEST, 2013

NAME:
CLASS: .

50 MARKS
TIME: 1 HOUR AND 30 MINUTES

1. An experiment was carried out to study the effect of different concentrations of sodium chloride
on red blood cells.

Diagram 1.1 shows the experiment set-up by a student.

Diagram 1.2 shows the red blood cells as seen under the microscope after being immersed in
0.15 M sodium chloride solution (test tube A) for 30 minutes.
The experiment was repeated by immersing the red blood cells in test tubes containing different
concentrations of sodium chloride.
Test tube B contains 0.30 M sodium chloride solution, test tube C contains 0.45 sodium chloride
solution and test tube D contains 0.60 M sodium chloride solution respectively.
The results are shown in Table 1.1.
Test tube

Concentration
of Red blood cells as seen under the Number of crenated
sodium chloride (M) microscope
red blood cells

0.30

0.45

0.60

TABLE 1.1
1

a) i) State two observations on the relationship between the number of crenated red blood
cells and the concentration of sodium chloride solution.
Observation 1:
____________________________________________________________________
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Observation 2:
____________________________________________________________________
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(3 marks)
ii) State the inference which corresponds to the observations in 1 (a) (i)
Inference from observation 1:
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_____________________________________________________________________
Inference from observation 2:
__________________________________________________________________
__________________________________________________________________
(3 marks)
b) Record the number of crenated red blood cells in the box provided in Table 1.
(3 marks)
c) Complete Table 1.2 based on this experiment.
Variable
Manipulated variable:

Method to handle the variable

-----------------------------Responding variable:
-----------------------------Constant variable :
-----------------------------(3 marks)

d) State the hypothesis for this experiment

_____________________________________________________________________
_____________________________________________________________________
(3 marks)
e) Construct a table and record all the data collected from this experiment in the space
below. Your table should have the following titles:
Test tube
Concentration of sodium chloride
Number of crenated cells

f)

i) Plot a graph showing the number of crenated red blood cells against the
concentration of sodium chloride on a graph paper.
(3 marks)
ii) Referring to the graph in 1(e) (i), describe the relationship between the number of
crenated red blood cells and the concentration of sodium chloride solution.
______________________________________________________________________
______________________________________________________________________
(3 marks)

g)

In another experiment, test tube B was left aside for 60 minutes. Predict the results of
the experiment.
______________________________________________________________________
______________________________________________________________________
(3 marks)

h) From this experiment, what can you deduce about osmosis?

_______________________________________________________________________
_______________________________________________________________________
(3 marks)
i)

The following information shows the phenomenon of cells in hypotonic and hypertonic
solutions.
Plasmolysis, crenated, deplasmolysis, turgid
Classify the words given above into Process and Appearance of cells according to the
phenomenon of cells in hypotonic and hypertonic solution in a table.

(3 marks)

2. The rate of photosynthesis is influenced by different environment factors.

Based on the above situation, plan a laboratory experiment to determine the effect of light
intensity on the rate of photosynthesis.
The planning of your experiment must include the following aspects:

Problem statement
Hypothesis
Variables
List of apparatus and materials
Experimental procedure
Presentation of data
(17 marks)

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