Croix Stone/Alexis Schorno Johnson Biology November 21, 2013

Growth Rate of Yeast

Introduction
In this lab e are testing !i""erent aci!s an! bases on yeast, e are trying to i!enti"y the e""ect on the gro th rate o" yeast hen the #$ o" the gro ing me!i%m is altere!&

Procedures
1& A!! 'm( o" 1) s%crose sol%tion to each t%be& (abel t%bes accor!ing yo%r assignment *aci! or base+ 2& A!! the a##ro#riate amo%nt o" base or aci! to each t%be& 3& ,i# a stri# o" #$ #a#er into each sol%tion an! recor! the #$ yo%r !ata table -& .eigh 0&2 grams o" yeast "or the other three t%bes& As soon as each t%be as the yeast a!!e! to it start the sto# atch& Wait three minutes for the yeast to multiply. You may wish to 30 seconds between tubes so you may take reading for the tubes e actly three minutes

after the yeast us added. /& Be"ore each t%be can be a!!e! to s#ectro#hotometer, you need to pour the solution into a special tube called cu!ette. ,o this at the very en!, right be"ore #lacing the c%vette into the s#ectro& .hile aiting a!! the control *no a!!ition t%be+ an! a!0%st the transmittance to 100)& 1eacher ill assist ith this ste#& 2& A"ter the 3 min%te ait, #lace each t%be in the sam#le com#artment, 3ecor! the #ercent light transmittance on the !ata chart& 4& Share yo%r !ata ith the teacher& '& Collect the class !ata& 5o% ill ise the class !ata to ans er the #ost lab 6%estions&

"aterial
7 7 7 7 7 7 8o%r test t%bes an! test t%be rac9 *%se ne clean t%bes:+ Small bea9er ith yeast ;ra!%ate! cylin!er ith 3m( #i#ette #$ #a#er Stir ro! *o#tional+ Bea9er o" 1) s%crose sol%tion

Post #ab $uestions

1& 1he #$ o" 2&4

2& 1he 1hree !ro#s o" Na<$ 3& =ore !angero%s aci!s !estroying the yeast

-& 1he #$ o" 2&'#$ ha! the most transmittance /& It staye! relatively similar to the other tests 2& $igher the #$ on the scale the more yeast gro th

%hallenge Problem
1he claims o" the ebsite match o%r !ata beca%se the 2&4 #$ ha! 100) transmittance hich means that it as li9e %s%al yeast& Another ex#eriment o%l! be have #$>s "rom -?2 an! see hich one ha! the most transmittance&