1 ..

. 152, 11527

/: . ..
.: 210 7702978, Fax : 210 7702978
URL: http://www.sotiria.gr/new/nrcm/index.htm
e-mail: vogia2@gmail.com

- . .

, 2012

.

,
, .

, ,

.

,

(SOPs),
,

.

. ..

. . . ,
, , ,
210-7763428, 210 -7702978, 3428, e-mail : vogia2@gmail.com

1. .
, / ,
2. . .
, / , -
3. . .
, / , -
3. .
, ., -
4. .
,.,/
5. .
, . , ,
6. . .
, ,
7. . .
, . , -
8. . .
, -
9. .
, - -

/
/

/
/

:
()
. ...
. 152 11527
/ 210-7702978 . : 3428
e-mail : vogia2@gmail.com

1. -:
210 7763301 3301 , .

2. ():
210 7779400, 210 7763302 .3302
e-mail: reflabtbsotiria@yahoo.gr

: 2107779400 .

3. -- :
210 7712028 3133 ,

4. /
:
210 7763567 3567. e-mail: reflabtbsotiria@yahoo.gr

5. :
210 7763301 3301

6. :
210 7733322 , 3322,3342

:
http://www.sotiria.gr/new/nrcm/index.htm
e-mail: reflabtbsotiria@yahoo.gr

vogia2@gmail.com

6
6
7
11
11
12

16

17

17
18

18

19

- -
(QUANTIFERON TEST)

21

23
23
24
25
26
27
28

14
16
16

16

21
21

28
28

29

,
ycoplasma , Ureaplasma
C.difficile

-

-
, - MIC-

- - MIC

- - MIC , ,



P. Carinii
Legionella , S.pneumoniae

M.tuberculosis

M.tuberculosis complex

- (QuantiferonTB test)


, ,
M. leprae


/ :
Adenovirus ()IgG IgM
RSV ( )IgG IgA
Varicella( ) IgG IgM
Coxsackie B1 B5 IgG IgM
EBV (VCA) IgG IgM, EBV (EBNA) IgG, EBV (Early Antigen)
Echo IgG IgM IgA
I IgG IgM, II IgG - IgM
Parainfluenza 1,2,3
Influenza A+B IgG - IgM
Rubbela IgG IgM
Coxiella burnetti phase I IgG IgA
Brucella IgG IgM IgA
Legionella pneumophila 1-7 IgG IgM
Mycoplasma pneumoniae IgG IgM IgA
Chlamydia pneumoniae IgG IgM IgA
Helicobacter pylori IgG IgM IgA
Borrelia (Lyme)IgG IgM
Leptospira IgG IgM
Listeria monocytogenes IgG IgM
Cryptosporidium
Leishmania
Cryptococcus

Entamoeba histolytica
Echinococcus
Aspergillus IgG IgM IgA, Aspergillus antigen(galactomannan)
Candida albicans IgG IgM IgA
Toxoplasma IgG IgM, Toxoplasma Avidity
Parvo B-19
(PCT)
CMV () IgG IgM.

IgE Total
F1( )
F2( )
F3()
F4()
F5()
F6()
F7( )
F8()
F9()
F10()
F12()
F13()
F14()
F15( )
F17()
F18( )
F19( )
F20()
F21()
F22()
F23()
F24()
F25()
F26( )
F27( )
F29()
F31()
F32( )
F33()
F35()
F36()
F38( )
F40()
F41()
F42()
F44()
F47()
F48()
F49()
F50()
F52( /)
F53( )
F67()
F68( )
F75( )
F76(ALPHA-LACTALBUMIN)
F77(BETA-LACTOGLOBULIN)
F78()
F79()
F80()
F81( CHEDDAR)
F82( MOLD)
F83()
F84()
F87()
F88()
F92()
F93 F52()
F94()
F95()
F96()
F114()
F179()
F180()
F182( )
F203()
F231( )
F235()
F256()
F299

F15( )
F17()
F18( )
F19( )
F20()
F21()
F22()
F23()
F24()
F25()
F26( )
F27( )
F29()
F31()
F32( )
F33()
F35()
F36()
F38( )
F40()
F41()
F42()
F44()
F47()
F48()
F49()
F47()
F48()
F49()
F50()
F52( /)
F53( )
F67()
F68( )
F75( )
F76(ALPHA-LACTALBUMIN)
F77(BETA-LACTOGLOBULIN)
F78()
F79()
F80()
F81( CHEDDAR)
F82( MOLD)
F83()
F84()
F87()
F88()
F92()
F93 F52()
F94()
F95()
F96()
F114()
F179()
F180()
F182( )
F203()
F231( )
F235()
F256()
F299
G1()
G2()
G3( )
G4( )
G5( )
G6()
G7()
G8( )
G9( )
G9( )
G10()

10

G1()
G2()
G3( )
G4( )
G5( )
G6()
G7()
G8( )
G9( )
H1( )
H2( )
I1( )
I2( )
I3( )
I4( )
I5( )
I6()
I8
I71()
I73()
I75()
T2( )
T3
T4(ANISAKIS SIMPLEX)
T6( )
T7( )
T8( )
T9()
T10()
T11()
T12()
T14()
T15()
T16( )
T18()
T19()
T20(MESQUITE)
T21()
T22()
T23( )
T24( )
25(OLIVE RUSSIAN)
33()
34()
1
E1( )
E2( )
E3( )
E5( )
E7( )
E71( )
E94( )
D1()
D2()
D3()
D70(ACARUS SIRO)
D71(LEPIDOGLYPHUS DESTUCTOR)
D72(TYROPHAGUS PUTRESCENTIAE)
D73(GLYCYPHAGUS DOMESTICUS)
D74()

G11(BROME GRASS)
G12(. )
G13( )
G14()
G15()
G16()
GX1(GRASS MIX 1)
GX2(GRASS SCREEN)
W1( )
W2( )
W3( )
W4( )
W5()
W6()
W7()
W8()
W9()
W10()
W11()
W12
W14(PIGWEED , ROUGH)
W15()
W18( )
W19(-)
W20()
W21()
M1(PENICILLIUM CHRYSOGENUM)
M2(CLADOSPORIUM HERBARUM)
M3(ASPERGILLUS FUMIGATUS)
M4(MUCOR RACEMOSUS)
M5(CANDIDA ALBICANS)
M6(ALTERNARIA TENUIS)
M7(BOTRYTIS CINERIA)
M9(FUSARIUM MONILIFORME)
M10(STEMPHYLIUM BOTRYOSUM)
M17(CLADOSPORIUM FULVUM)
M19(ASPERGILLUS VERSICOLOR)
M29(ASPERGILLUS REPENS)
C1( G)
C2( V)
C3(CHYMOPAPAIN)
C201()
C203()
C204()
C211()
C212()
C217()
C231(ARTICAINE)
C283(NAPROXEN)
C295(STREPTOMYCIN)
C299(ESSKASTANIE)
82 (LATEX-U.S. w/D.I.)
P1 ()
2 ()
3 ( )

10

11

,
.
,

(. . 3450 2107750349)

(. 213 2040471-473)
() (. 210 - 8921004005).

(Total IgE, RAST

TEST)
.
IgE 5-10 ml
IgE .

.. ,

,
.
: ,
.

11

12

,




,

,

, , ,
() , ,
, ,


,
,

, , , , ,
, , ,
, stress,
, ...
, ,
( ) .. ,
, , ,
, , (), ,
, , , (
12

13

, )
.. ( ), , ,
, , ,



, .. ,
, ,
, , stress, ,
....

13

14

Propionibacterium spp.
Corynebacterium spp. ()
Acinetobacter spp.
Staphylococcus spp.
Micrococcus spp.
Fusobacterium spp.
Bifidobacterium spp.
Neisseria spp.
Clostridium spp.
Enterobacteriaceae
Candida spp.
Peptostreptococcus spp.
Bacillus spp.
Mycobacterium spp. (
)

Streptococcus spp.
Staphylococcus spp.
Neisseria spp.
Corynebacterium spp. ()
Enterobacteriaceae
Moraxella spp.
Micrococcus spp.
Fusobacterium spp.
Peptostreptococcus spp.
,
,
,

Streptococcus spp.
Staphylococcus spp.
Pseudomonas spp.(
)
Neisseria spp. ( )
Corynebacterium spp. ()

Enterobacteriaceae
Haemophilus spp.
Candida spp.

Streptococcus spp.
Staphylococcus spp.
Enterobacteriaceae
Pseudomonas spp.
Candida spp.
Peptostreptococcus spp.

(pH 2-3)
(0-10)/ml)

.

Staphylococcus spp.
Enterococcus spp.
Bacillus spp.
Candida spp.
Bacteroides spp.
Fusobacterium spp.
Clostridium spp.
Eubacterium spp.
Peptococcus spp.
Peptostreptococcus spp.
Lactobacillus spp.
Bifidobacterium spp.
Veillonella spp.
Enterobacteriaceae
Acinetobacter spp.
Pseudomonas spp.
Entamoeba coli
Endolimax nana
Dientamoeba fragilis
Iodamoeba butschlii
14

15

Trichomonas hominis

Lactobacillus spp.
Bacteroides spp.
Corynebacterium spp. ()
Enterococcus
Enterobacteriaceae
Micrococcus spp.
Clostridium spp.
Peptostreptococcus spp.
Bifidobacterium spp.
Moraxella spp.
Acinetobacter spp.
Candida albicans
Gardnerella vaginalis
Chlamydia spp.
Mycoplasma spp.

Staphylococcus spp.
Corynebacterium spp. ()
Streptococcus spp. ( )

Neisseria ( )
Enterobacteriaceae
Chlamydia spp.
Mycoplasma spp.
Candida spp.
Lactobacillus spp.
Gardnerella vaginalis

Streptococcus spp.
Staphylococcus spp., Group
Streptococcus (GBS)

15

16

1) .
2) ().
3) .
4)
5) .
6) .
7) .

1) , , .
2) .
3) .
4) .
5) .

1) .
2) .
3) .
4) .
5) .
6) .
7) .
8) .
9) .
10) .
11) .
12) (, , ).
13) .
14) .
15) Gram
(1 %).
16) .
17) .

70%

2 min

16

17

BACTEC PLUS Aerobic

BACTEC PLUS Anaerobic

MYCO/F LYTIC

5-10 ml

,

, ,

: 3 10

: 3
1-2

: 3
24 . , 3 .

: 3
1 . 24 ,
3 .

: 2 12
.

1 ml

()

MYCO/F LYTIC
EDTA,

( ,,
, ,
...)

17

18

(
2 ).

,
Gram.

,
Gram.

,
70%
, ,
.

:

5 cm .

:
, ,
.

.

( - -
- )

70%

: 2-2.5 ml
EDTA

() : 1 ml

() : 1 ml
(BACTEC PLUS Anaerobic)

: 1 ml

3 :
: 1-1.5 ml EDTA

(-):0.5-1ml

: 2 ml
18

19

: 0.5 ml

,
.

(
).

,
.

1 2
,
.

.
:1) Corynebacterium diphtheriae Neisseriae
gonorrhoeae, Bordetella pertusis, Borrelia spp., Fusobacteria spp.,
.2)
Staphylococcus aureus Neisseriae meningitidis . 3)

.

S. aureus -
(Group A Streptococcus G. A. S.):1)
.2) 2
cm . . 3)
.4)
.

:1) 1, 2, 3 .2) 2 - 3
.
:-
,
.-
S. aureus G. A. S.
.

() :
.
19

20

, .
.
, . ,
.

Pneumocystis
carini Legionella spp.
:

( , ..).

.
:

,
5ml.

()
.
.
, (PROTECTED
BRUSH), BRONCHIAL WASHING,
(BRONCHOALVEOLAR LAVAGE BAL)

,
.

:
.
.

1)

Darcon, .

20

21

2)

, ,
.
:
(.. zygomycetes)
,
.

,

.

/

.

-

( QUANTIFERON TEST)

3 ml 1 ml
(. )

>5 ml 3 .
- 12.00

- 3.00 ..
- > 4 ,
3 ml aHCO3 100
mg/ml .

1.

- (
) (~ ). -
:

21

22

. 6
.
-
.
:1) (
foil) .
.2) , ,
.3) 1 - 2
( ). 4)
.5)
Taenia spp. 4 5 .
2.
() (
, ).
.
1 .
: , ,
.
3.

-
( ) ,
1/2 .
- .-
..
, ,
, , ..
:1) . .
foil ,
.
. 2) .3) :
,
.4) Cryptosporidium
spp, Y. enterocolitica, Vibrio spp Aeromonas spp
.
4.
C. difficile
1-2 ml
.
2
2 .

22

23

( ). .
.
5-10 ml . .

1 ,
2 .
:

.
,
.

:
, 5 cm
.

,
. Folley
.
.
-Koch :

2 .

1.
- .
- . 0,5 ml . .
- .
- .
-
.
2.
:
. N. gonorrhoea (' ).
. ( )
.

23

24

.
.
. .
. C. trachomatis M. hominis
, .

.
.
3. -.Koch
. ,
.
. .
4.
. (Betadine).
. .
.

.

1. .
- 2 - 4
, 1 - 2 sec.
- 0, 5 ml . .
- ,
.
- C. trachomatis M. hominis
, .

.
:1) .2)
3 - 4 .3)
N. gonorrhoea
, . N.
gonorrhoea .
1. . 5
Stamey - Meares.
:
. VB1 (Voided Bladder1) :
5-8 ml).
. VB2 (Voided Bladder2) 10 ml :
.
. EPS (Expressed Prostatic Secretions) .

24

25

.
.
.VB3 (Voided Bladder3) 2-3 ml
.

I.
. 70 %
(Betadine). 1 - 2 min.
70 % .
. (3 - 5 ml) (22 - 23 g)
.
.
. ,

.
.
.
. 2 - 3 .
:1)
. 2) M.leprae

II.
. 70 %
(Betadine).
. 2 min.
. 70 % .
. 3 - 4 cm
.
:1)
.2)
2 x 1 cm ( 500 mg),
.
.
III.
. 70 % .
. (Betadine) x 1 - 2 min..
70 % .
. .
. .
. ,
.
:
.
25

26

IV. / -
. 70%
.
. (Betadine) x 2min.
. 70% .
. /
,
.
.
.
. .
. ,
.
:1) 3 - 4
( )
, .2)
2
x 1 cm ( 500 mg),
.
.
V.
. (H2O).
.
, .
.
petri .
. .
VI. ()
. .
. ~ 10 .
. . . .
. .
.
V.
. 70 % .
. :
. .
. .
.
. .
. .

I.
26

27

. ( )
.
. 5 - 10
.
. .
. .
:- (..
, ...),
.
II.
( ) .
. .
. .
.
.
. .
:1) ,
.2)
,
. 3) .. ,
, , ...

I.
.
. . ..
, ..
..
..
Gram Giemsa.
:1)
Kimura.2)

.
II.
. (1 - 2 )..
Kimura

. 2 .
:1) .2)
, .3)
Gram .
4) Acanthamoeba
.
27

28

5) .
.
III. . o
..

.
/
/
5ml

-
- ( 10-15 )

:
1. ,
:


.
ZiehlNeelsen.

.
. ,

Ziehl-Neelsen.
. , ,
., .
2. ,
,
:
( )
.

28

29

1.
.
. , .
2.
- .
. , , 1988.
3.

. , , 2001.
4.

. ..
.
5.
Lynne S. Garcia. Clinical Microbiology Procedures Handbook third edition and
2007 update,
5.
, .. - .. 2002,

6.
HPA: National Standard Methods, Guidance Notes and Algorithms, Health
Protection Agency 2006
7. Center for Disease Control and Prevention, National Institute for Occupational
Safety and Health February 2007, posting date. Biosafety in Microbiological and
Biochemical Laboratories , 5th edition, Washington, DC.
8.
CLSI 2005. Protection of Llaboratory Workers from Occupationally Acquired
Infections . Document M29-A3. CLSI , Wayne, PA.
9.
Sewell, D. L. 2004. Nosocomial infections in diagnostic llaboratories, p. 14311441. In C. G. Mayall,(ed.), Hospital Epidemiology and Infection Control, 3th edition.,
Lippincott Williams and Wilkins, Philadelphia., PA.
10.
,. . , ., .,
2006
11.
Murray P.R. Manual of Clinical Microbiology, 2003
12.
Isenberg HB. Clinical Microbiolgy Procedures Handbook ASM, Washington,
1992
13.
Miller jm . Aguide to specimen management in Clinical Microbiology ASM .
Washington,1999
14.
Koneman E., Allen S., Janda W. , Schreckenberger P., Winn W., Woods G.,
Procop G. Color Atlas and Textbook of Diagnostic Microbiology 6th Ed. Lippincott,
2005

29

..... ,

,
()
, ,

,
.
.
, email,
.....
:
. 152, 11527

reflabtbsotiria@yahoo.gr
: 2107779400

.. / .. /..

2
3

6
7
8

;

;


;

(.
);

;


;

;

;

, :

:
():

:
:

31

(Mycobacterium Direct Testing, MTD)

1. Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test (AMTD)
rRNA Mycobacterium tuberculosis complex (MTBC)
, .

2.
86% 97%, , .
93% 99%, 69% 97%.
66% 96%, .

3.
. ,
.

(~85% ).

4. ,
.

5.
.
(CDC), / ,
.
.
, 2 ,
.

6.
(3-7% ):
. , .

.
. ,
.

/ .

7.

.
,
.

8. ( , , )
.
0.5 ml. H
.

9. AMTD
, AMTD :
1. AMTD .
2. M. tuberculosis.
3. 7 12 .

(. 2107763567: . , . )

31

32

:
o

12

5-6 8:00 8:30

8-FR

5-10 ml

, 5 ml
water for injection ( NaCl) 10 kg

3 Ml NaHCO3 100 mg/ml.

,

4
( , )

32

33

-
/: . ..
. 152, 11527
& Fax: 210 7779400
Url: http://www.sotiria.gr/new/nrcm/index.htm
e-mail: reflabtbsotiria@yahoo.gr

- Elisa

QuantiFERON-TB Gold-IT

H

.
.
. ..

. ..

________________________________ /
__________________________
/ ________________________
: ______________________

__________________________

:_____________________________________________

BCG;

;
,
:

(13-14)

p.o.

Mantoux:

:();__________________

; HIV ;
;
; ; _______________

Mantoux

;;

__________
__________ mm

-
;

;:
; , ; ___________
;

(;)_______________________

______________________________________________________
____________________________ ___________________________
:

33

34

:
1. 1mL 3 QuantiFERON-TB Gold IT
(, , ).

1mL.

QuantiFERON-TB 0.8 1.2mL.

, . .

!
.
2. 8 10 - 5 ,
.
.
3. (, /, )
4. ,
. .
, :

16 17-27 C (:

, 9:00 13:00)

, , 16-20 37 C.
CO2. 20 . ,
.

34

35

35