Plot Lineweaver Burk L-Asparaginase dari Penicillium cyclopium

Fig. 3: Lineweaver Burk plot for the purified Penicillium cyclopium

L-asparaginase.

The substrate affinity of L-asparaginase measured by Km value and Vmax were found to be 0.3 mM
and 3333U/ mg protein respectively as determined by Lineweaver Burk plot (Figure 3).
Perhitungan :

y = ax + b

Nilai Vmaks b =

b=
b = 0.0003

Nilai Km a =

a=
Km = 1000
y = 1000x + 0.0003

Plot Michaelis-Menten L-Asparaginase dari Streptomyces griseoluteus

[S]

0.01
0.02
0.03
0.04
0.05
0.06
0.07
0.08
0.09

1.98
3.46
4.42
5.12
5.56
5.92
5.96
5.96
5.96

Figure 5: Michaelis- Menten plot of L-asparaginase

Nilai Vmaks
Vmaks = 5.96

Nilai Km = [S]

Vmaks

Km = ...
Plot Lineweaver-Burk L-Asparaginase dari Streptomyces griseoluteus

Figure 6: Lineweaver- Burk double reciprocal plot of

Streptomyces griseoluteus L-asparaginase

From the LB plot the equation y=0.0039x + 0.1032 gives 1/Vmax value 0.103 and 1/Km value 26.525. So the
calculated values of Km and Vmax values were 0.0377M and 9.69 IU/mL*min respectively and the results were
shown in Figure 6.

Perhitungan :

y = ax + b
y = 0.0039x + 0.1032

Nilai Vmaks b =

0.1032 =
Vmaks = 9.6899

Nilai Km a =

0.0039 =
Km = 0.03779

[S]
0.01
0.02
0.03
0.04
0.05
0.06
0.07
0.08
0.09
Perhitungan :

y = ax + b

V
1.98
3.46
4.42
5.12
5.56
5.92
5.96
5.96
5.96

1/[S]
100
50
33.33
25
20
16.67
14.29
12.5
11.11

1/V
0.51
0.29
0.23
0.20
0.18
0.17
0.17
0.17
0.17

y = 0.0039x + 0.1082

Nilai Vmaks b =

0.1082 =
Vmaks = 9.242

Nilai Km a =

0.0039 =
Km = 0.036