CLASSIFICATION OF ENZYMES

(Enzyme Comission-EC)

EC 1. OXIDOREDUCTASES

EC 1 enzyme Type of redox Possible reaction type

reaction

Succinate- C=C formation

dehydrogenase
(citric acid cycle)

Alcohol- Alcohol-aldehyde
dehydrogenase conversion

Aldehyde- Aldehyde-acid
dehydrogenase conversion

Phe hydroxylase
(monooxygenase)

EC 2. TRANSFERASES

EC 2 enzyme Transferred group Possible reaction type

Acyl-transferase Acetyl, succinyl,

(eg. synthesis of aminoacyl
triacylglycerols and
phospholipids)

Phospho- -H2PO3 (phosphoryl)

transferase
(eg. hexokinase,
glucokinase)

Amino- transferase -NH2 (amino)

(eg. ASAT, ALAT)

Sulfo- transferase -SO3H (sulfuryl)

(eg. GAG synthesis)
EC 3. HYDROLASES

E.C. 3 enzyme Substrate bond Possible reaction type

Esterases (eg.
Lipase, neutral fats
phospholipase, phospholipids ester
acetylcholine acetylcholine
esterase)
Phosphodieste-
rases (eg. cAMP Nucleotides phospho-
phosphodieste- (cAMP – 5’ AMP) diester
rase)
Phosphatases phopho-
phosphatester
(eg. glucose-6- mono-
(glu-6-P)
phosphatase) ester

Glycosidases
polisaccharide
(eg. amylase, glycoside
disaccharide
lipase)

Proteases
proteines
Peptidases peptide
peptides
(eg. tripsin)

EC 4. LIASES

Removed
E. C. 4…enzyme Possible reaction type
group
Decarboxylase
(eg. amino acid CO2
decarboxylase)
Aldolase
Aldehyde
(eg. aldolase A/B)
Synthase
(eg. Gly synthase) CO2 + NH3 Gly

Dehydratase
(eg. cysthationin H2 O
synthase)
Desaminase
NH3
(eg. Gln desaminase)
EC 5. ISOMERASES

Changing
E. C. 5… Isomera-
position of Possible reaction type
enzyme ted group
the group

Glucose-6-
phosphate- carbonyl C-1 ‹―› C-2
isomerase

Phosphoglycerate
phosphoryl C-2 ‹―› C-3
-phosphomutase

Epimerase
(eg. UDP-Glu hydroxyl D↔L
UDP-Gal)

EC 6. LIGASES

E.C. 6... enzyme Type of bond Example

Activation of amino acid, synthesis of
Aminoacyl-tRNS-synthetase C―O
proteins
Glutamine- synthetase C―N Biosynthesis of glutamine

Amino acid-ligase C―N Synthesis of proteins

Fatty acid synthesis (malonylSCoA
Acetyl-CoA-carboxylase C―C
formation)