winCATS Planar Chromatography Manager

Analysis Report
Method D:\TLC SCANNER\My Documents\CAMAG\METODE\Scan
PG.cme
Created by FITOKIMIA Wednesday, December 29, 2010
1:22:22 PM
Last modified by Biologi Farmasi UGM Wednesday, October 08, 2014
11:09:44 AM
SOP document
Validated Design
Description :

Analysis D:\TLC SCANNER\My Documents\CAMAG\DATA\Prak AKTO

2014\G1\20141008-Jeru
k Purut Hidrolisis K1.cna

Created/used by Biologi Farmasi UGM Wednesday, October 08, 2014

11:18:11 AM
Current user Biologi Farmasi UGM

Stationary phase
Executed by Biologi Farmasi UGM Wednesday, October 08, 2014
11:18:09 AM
Plate size (X x Y) 10.0 x 10.0 cm
Material TLC plates silica gel 60 F 254
Manufacturer E. MERCK KGaA
Batch
GLP code
Pre-washing No
Modification No

Definitions - Screening
Executed by Biologi Farmasi UGM Wednesday, October 08, 2014 11:10:36 AM
Batch Product
Substance name Rf Window size Manufacturer number Expiry date number

AutoGenerated1 0.01 0.500

AutoGenerated2 0.26 0.500
AutoGenerated3 0.38 0.500
AutoGenerated4 0.13 0.500

Detection - CAMAG TLC Scanner 3

Information
Application position 10.0 mm
Solvent front position 90.0 mm

Instrument CAMAG TLC Scanner 3 "Scanner3_140718" S/N 140718 (1.14.28)

Executed by Biologi Farmasi UGM Wednesday, October 08, 2014 11:18:09 AM
Number of tracks 1
Position of first track X 66.6 mm
Distance between tracks 0.0 mm
Scan start pos. Y 4.8 mm
Scan end pos. Y 46.0 mm
Slit dimensions 8.00 x 0.40 mm, Macro
Optimize optical system Light
Scanning speed: 20 mm/s
Data resolution: 100 m/step

User : Biologi Farmasi UGM Approved :. . . . . . . . . . . . . . . . . . . SN 1410W025, V1.4.3

Wednesday, October 08, 2014 11:19:13 AM Report ID : 07DE0A08040B120B Page 1 of 5
 winCATS Planar Chromatography Manager

Measurement Table
Wavelength 254
Lamp D2 & W
Measurement Type Remission
Measurement Mode Absorption
Optical filter Second order
Detector mode Automatic
PM high voltage 303 V

Detector properties
Y-position for 0 adjust 4.8 mm
Track # for 0 adjust 0
Analog Offset 10%
Sensitivity Automatic (39)

Integration
Properties
Data filtering Savitsky-Golay 7
Baseline correction Lowest Slope
Peak threshold min. slope 5
Peak threshold min. height 10 AU
Peak threshold min. area 50
Peak threshold max. height 990 AU
Track start position 6.6 mm
Track end position 44.7 mm
Display scaling Automatic
All tracks at Wavelength

1000
[ AU ]
800
700
600
500
400
300
200 100
100 [ mm ]
0 80
-0.05 70
0.00 60
0.05
0.10 50
0.15 40
0.20
30
0.25
0.30 20
0.35 10
[ Rf ]
0.45 0

User : Biologi Farmasi UGM Approved :. . . . . . . . . . . . . . . . . . . SN 1410W025, V1.4.3

Wednesday, October 08, 2014 11:19:13 AM Report ID : 07DE0A08040B120B Page 2 of 5
 winCATS Planar Chromatography Manager
Track 1, ID:
00 1000

00 900

00 800

00 700

00 600

00 500

00 400
AutoGenerated1 @ 254 nm
00 300 AutoGenerated2 @AutoGenerated3
254 nm @
AutoGenerated4 @ 254 nm
00 200

00 100

0 0
-0.13 -0.03 0.07 0.17 0.28 0.38 0.47 -0.13 -0.03 0.07 0.17 0.28 0.38 0.47
Start Start Max Max Max End End Area
Peak Rf Height Rf Height % Rf Height Area % Assigned substance
1 -0.03 76.6 0.01 229.4 29.33 0.08 0.0 12238.7 30.33 AutoGenerated1
2 0.08 1.5 0.13 170.2 21.76 0.20 12.0 9556.4 23.68 AutoGenerated4
3 0.20 12.2 0.26 192.1 24.55 0.32 2.8 9642.1 23.89 AutoGenerated2
4 0.33 0.2 0.38 190.5 24.35 0.43 2.8 8917.1 22.10 AutoGenerated3

Spectrum scan
Executed by Biologi Farmasi UGM Wednesday, October 08, 2014 11:14:12 AM
Mode All detected peaks
Slit dimensions 8.00 x 0.40 mm, Macro
Optimize optical system Resolution
Scanning speed 100 nm/s
Data resolution 1 nm/step
Reference spectrum, pos X 66.6 mm
Reference spectrum, pos Y 4.8 mm

Measurement Table
Lamp D2 & W
Start wavelength 200 nm
End wavelength 500 nm
Measurement type Remission
Measurement Mode Absorption
Optical filter Second order
Detector Mode Automatic

Detector properties
Y-position for 0 adjust 0.0 mm
Track # for 0 adjust 0
Analog Offset 10%
Sensitivity Automatic (112)

AutoGenerated1 on all Tracks

100.0 100.0
[ AU ] [ AU ]
80.0 80.0
70.0 70.0
60.0 60.0
50.0 50.0
40.0 40.0
30.0 30.0
20.0 20.0
10.0 10.0
0.0 0.0
200.0 250.0 300.0 350.0 400.0 [ nm ] 500.0

User : Biologi Farmasi UGM Approved :. . . . . . . . . . . . . . . . . . . SN 1410W025, V1.4.3

Wednesday, October 08, 2014 11:19:13 AM Report ID : 07DE0A08040B120B Page 3 of 5
 winCATS Planar Chromatography Manager
T Rf Substance Max. @
1 0.01 Rf AutoGenerated1 402 nm

AutoGenerated2 on all Tracks

100.0 100.0
[ AU ] [ AU ]
80.0 80.0
70.0 70.0
60.0 60.0
50.0 50.0
40.0 40.0
30.0 30.0
20.0 20.0
10.0 10.0
0.0 0.0
200.0 250.0 300.0 350.0 400.0 [ nm ] 500.0

T Rf Substance Max. @
1 0.26 Rf AutoGenerated2 402 nm

AutoGenerated3 on all Tracks

100.0 100.0
[ AU ] [ AU ]
80.0 80.0
70.0 70.0
60.0 60.0
50.0 50.0
40.0 40.0
30.0 30.0
20.0 20.0
10.0 10.0
0.0 0.0
200.0 250.0 300.0 350.0 400.0 [ nm ] 500.0

T Rf Substance Max. @
1 0.38 Rf AutoGenerated3 402 nm

AutoGenerated4 on all Tracks

100.0 100.0
[ AU ] [ AU ]
80.0 80.0
70.0 70.0
60.0 60.0
50.0 50.0
40.0 40.0
30.0 30.0
20.0 20.0
10.0 10.0
0.0 0.0
200.0 250.0 300.0 350.0 400.0 [ nm ] 500.0

T Rf Substance Max. @
1 0.13 Rf AutoGenerated4 402 nm

Evaluation results

Evaluation Sequence
Track Track type Vial Sample ID
1 Sample 1

User : Biologi Farmasi UGM Approved :. . . . . . . . . . . . . . . . . . . SN 1410W025, V1.4.3

Wednesday, October 08, 2014 11:19:13 AM Report ID : 07DE0A08040B120B Page 4 of 5
 winCATS Planar Chromatography Manager

Table of substances
Position Tracks
Substance MD mm 1
AutoGenerated1 11.1 A
AutoGenerated2 30.5 A
AutoGenerated3 40.7 A
AutoGenerated4 20.7 A

User : Biologi Farmasi UGM Approved :. . . . . . . . . . . . . . . . . . . SN 1410W025, V1.4.3

Wednesday, October 08, 2014 11:19:13 AM Report ID : 07DE0A08040B120B Page 5 of 5