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Antibiotika Terhadap S.aureus: Data Pengamatan Dan Perhitungan A. Data Pengamatan
Antibiotika Terhadap S.aureus: Data Pengamatan Dan Perhitungan A. Data Pengamatan
A. Data Pengamatan
1. Pengamatan Diameter Hambat Antibiotik Terhadap Bakteri S.aureus dan
E.coli
Kadar Antibiotika Diameter Hambatan ±SD (mm)
(µg/cakram S.aureus E.coli
kertas) Ampisili Tetrasiklin kloramfeniko Ampisilin Tetrasiklin kloramfenikol
n l
5 9,67 28,25 1 21 30,8 -
2,5 - 26 11 16,5 27,6 -
1 1 - 10 15,75 26,5 -
0,5 - - 9 0,5 24,3 -
0,25 1 - 12 - 21 -
0,1 2 - 10 - 19,6 -
0,01 - - - - - -
Kontrol - - - - - -
a. Nilai KHM
Nilai KHM S.aureus Ampisilin : 25 µg/cakram kertas
Nilai KHM S.aureus Tetrasiklin : 250 µg/cakram kertas
Nilai KHM S.aureus Kloramfenikol: 50 µg/cakram kertas
Nilai KHM E.coli Ampisilin : 50 µg/cakram kertas
Nilai KHM E.coli Tetrasiklin : 10 µg/cakram kertas
Nilai KHM E.coli Kloramfenikol :-
25
20
15
10
5
0
500 250 100 50 25 10 1 Kontrol
Konsentrasi (µg/cakram)
35
Diameter Hambatan (mm)
30
25
20
15
10
5
0
500 250 100 50 25 10 1 Kontrol
Konsentrasi (µg/cakram)
B. Perhitungan
Persediaan antibiotik di Laboratorium :
1.000.000 µg
1 g/ 5 mL = 1.000.000 µg / 5 mL ¿ =200.000 µg /mL
5 mL
Larutan induk = 1000 µg /mL dalam 100 mL
V1.N1 = V2.N2
V1. 200000 = 100. 1000
100.000
V1 = =0,5 mL
200.000
1. Difusi Agar
500 µg/mL
V1.N1 = V2.N2
V1. 1000 = 100. 500
50.000
V1 = =50 mL
1000
250 µg/mL
V1.N1 = V2.N2
V1. 1000 = 100. 250
25.000
V1 = =25 mL
1000
100 µg/mL
V1.N1 = V2.N2
V1. 1000 = 100. 100
10.000
V1 = =10 mL
1000
50 µg/mL
V1.N1 = V2.N2
V1. 1000 = 100. 50
5.000
V1 = =5 mL
1000
25 µg/mL
V1.N1 = V2.N2
V1. 1000 = 100. 25
2500
V1 = =2,5 mL
1000
10 µg/mL
V1.N1 = V2.N2
V1. 1000 = 100. 10
1000
V1 = =1mL
1000
1 µg/mL
V1.N1 = V2.N2
V1. 1000 = 100. 1
100
V1 = =0,1 mL
1000
2. Pengenceran Agar
0,9 µg/mL
V1.N1 = V2.N2
V1. 1000 = 100. 0,9
90
V1 = =0,09 mL
1000
1,8 µg/mL
V1.N1 = V2.N2
V1. 1000 = 100. 1,8
180
V1 = =0,18 mL
1000
3,6 µg/mL
V1.N1 = V2.N2
V1. 1000 = 100. 3,6
360
V1 = =0,36 mL
1000
7,2 µg/mL
V1.N1 = V2.N2
V1. 1000 = 100. 7,2
720
V1 = =0,72 mL
1000