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Experiment 8 Ammonia Nitrogen PDF
Experiment 8 Ammonia Nitrogen PDF
CMT 565
Introduction
Ammonia is a product of the microbiological decay of animal and plant protein. It can be
directly reused by plants to produce protein. Ammonia and nitrogen compounds are applied
directly as fertilizers. The presence of ammonia nitrogen in surface water usually indicates
domestic pollution. High concentrations of these nutrients may indicate a well construction
problem or an environmental impact in the vicinity of the water supply.
Objectives
Apparatus
Reagents/ chemicals
Mineral stabiliser, Nessler reagent, polyvinyl alcohol dispersing agent, nitrogen ammonia
voluette ampule standard (50 mg/L), nitrogen ammonia standard solution (1mg/L), distilled
water
Procedure
For sample preparation, 1mL of sample was added in the 25mL volumetric flask and filled
with distilled water to the mark. Distilled water was added to the mark at another volumetric
flask for blank preparation. Three drops of mineral stabiliser was added to each cylinder and
inverted several times to mix it. Then three drops of polyvinyl alcohol dispersing agent was
added to each cylinder and inverted several times also. 1.0 mL of Nessler reagent was pipette
into each cylinder. The cylinder was stopper and inverted several times to mix it. The
instrument timer was used to start a one-minutes reaction period to begin. After one-minutes
reaction, 10mL of each solution was poured into the sample cell. When the timer beeps the
blank solution was place into the cell holder and ZERO the instrument. The same procedure
was repeat for sample and standard solution but READ the instrument in mg/L NH3-N.
Result
Calculation
=229 mg/L
Accuracy check
M1V1 = M2V2
(1000)V1 = 2 (100)
V1 = 0.2mL
Value from Spectrophotometer = 1.53 mg/L
Discussion
From the experiment, we found that there are various reading of the ammonia after being
detected by spectrophotometer. The highest reading that have recorded is >3.50 mg/L (out of
range) due to high concentration ammonia in that water sample. After the water sample
diluted with 50 and 100 diluting factor the value is about at 4.75 mg/L and 2.29 mg/L
respectively. According to the typical ammonia value (0-2.5 mg/L) our data represent the
value required. Our sample in consist of high level of ammonia concentration that will harm
the aquatic animal.
The high concentration of ammonia in the water greater than 5 mg/L will cause excessive
growth of algae and other plants, leading to accelerated eutrophication or 'aging' of lakes, and
occasional loss of dissolved oxygen. Animals and humans cannot use inorganic forms of
nitrogen, so nitrate is not a nutrient for us. In most fish, ammonia is excreted by passive
diffusion of NH3 across the gills according to its partial pressure gradient (Wilson et al.
1998). Disruption of this gradient causes internal ammonia concentrations to increase,
affecting internal organs, nervous system function, and respiration. If nitrate-nitrogen exceeds
10 milligrams per liter in drinking water, it can cause a condition called methemoglobinemia
or "blue baby syndrome" in infants. In addition to prevention, drinking-water providers may
use advanced treatment techniques to remove nitrate from water. For example, Des Moines
Water Works uses advanced ion-exchange technology to remove excess nitrate and remain
below the 10 mg/L standard.
Conclusion
References