QUESTION ABOUT THE PPP (hexose monophosphate shunt pathway)

SECTION A (MCQs)

1. Two important by products of HMP shunt are

(A) NADH and pentose sugars

(B) NADPH and pentose sugars

(C) Pentose sugars and 4 membered sugars

(D) Pentose sugars and sedoheptulose

2. The following enzyme is required for the hexose monophosphate shunt pathway:

(A) Glucose-6-phosphatase

(B) Phosphorylase

(C) Aldolase

(D) Glucose-6-phosphate dehydrogenase

3. Dehydrogenase enzymes of the hexose monophosphate shunt are

(A) NAD+ specific

(B) NADP+ specific

(C) FAD specific

(D) FMN specific

4. One molecule of glucose gives ______molecules of CO2 in one round of HMP shunt.

(A) 6

(B) 1

(C) 2

(D) 3

5. Transketolase activity is affected in

(A) Biotin deficiency

(B) Pyridoxine deficiency

(C) PABA deficiency

(D) Thiamine deficiency

6. Reduced glutathione functions in R.B.Cs to

(A) Produce NADPH

(B) Reduce methemoglobin to hemoglobin

(C) Produce NADH

(D) Reduce oxidizing agents such as H2O2

True or false questions

1. All of the following statements about the pentose phosphate pathway are true EXCEPT

A) Its two functions are to produce NADPH and ribose-5-P

B) It uses glucose-6-P as a substrate when producing NADPH and CO2

C) Glucose-6-phosphate dehydrogenase is the control enzyme and it is regulated by the NADPH

concentration of the cell

D) If it is producing more than twice as much NADPH as ribose-5-P, it can produce glyceraldehyde-3-
P and fructose-6-P

E) It is found in the mitochondria of liver, muscle and brain but is absent from most other tissues of
the body

2. Your patient, Al Martini, has glucose-6-phosphate dehydrogenase deficiency as a result of taking a

sulfa drug. He has never been diagnosed with this disease before. He suffers from hemolysis while
his white blood cells look OK. You feel certain that the following statements are true EXCEPT

A) He has a fairly common genetic disease

B) This is probably the first time his system has been challenged with a lot of free radicals (reactive
oxygen species)

C) He has some glucose-6-phosphate dehydrogenase activity but less than most people

D) Most cells were not lysed because those cells had other ways to produce NADPH

E) His red blood cells have no trouble making reduced glutathione but cannot oxidize it

3. Which of following statements is NOT true? If a cell needs to make ribose-5-phosphate but the
concentration of NADPH is very high, the cell will

A) Use the 6-phosphogluconate dehydrogenase reaction

B) Use the nonoxidative portion f the Pentose Phosphate Pathway

C) Use the nonoxidative portion of the hexose monophosphate shunt

 D) Make ribose-5-phosphate using fructose-6-phosphate and glyceraldehyde

E) Make ribose-5-phosphate from glycolytic intermediates

4. NADPH is used as a substrate for all of the following EXCEPT

A) Malate dehydrogenase

B) Glutathione reductase

C) Fatty acid synthesis

D) Cytochrome P450 monooxygenases

E) Deoxynucleotide synthesis

5. All of the following enzymes and metabolites are found in the pathway for the reduction of HOOH
except

A) 6-phosphogluconate dehydrogenase

B) NADH + H+

C) Glutathione reductase

D) Reduced glutathione

E) Glutathione peroxidase

Section B (Short answer questions.

1. State the purpose(s) of the pentose phosphate pathway (hexose monophosphate shunt) in
metabolism; in which organ is this pathway most active?
2. show the structures (reactants and products) of either the oxidative stages of the pentose
phosphate pathway or of the transaldolase reaction (including enzyme bound intermediate)
3. What compounds control the PPP?
4. A deficiency in G6P dehydrogenase leads to which condition? How can this be a good thing?
5. What is ribose-5-phosphate generated in the PPP used for?
6. What compounds control the PPP?