Eprogram Book HSS 2019 - Versi Online - 2 PDF

The 9th International Symposium for Sustainable Humanosphere (ISSH)
Bogor, 28 -29 October 2019
Organizer
General Coordinator:
 Dr. Dede Heri Yuli Yanto, M.Agr.
Advisory Board:
 Prof. Dr. Sulaeman Yusuf, M.Agr.
 Prof. Dr. Ir. Subyakto, M.Agr.
 Prof. Dr. Bambang Subiyanto, M.Agr.
 Dr. Wahyu Dwianto, M.Agr.
 Dr. Ir. Euis Hermiati M.Sc.
 Taswin, S.IP., S.E., M.M.
Chairman:
 Dr. Ikhsan Guswenrivo, M.Sc.
Vice Chair:
 Dr. Didi Tarmadi M.Si.
Secretariat:
 Apriwi Zulfitri, M.Sc.
 Fahriya Puspita Sari, S.T.
 Linda Kriswati
 Bernadeta Ayu Widyaningrum, M.Si
Treasury and Meal:
 Erlin Herlinawati, S.E.
 Dwi Ajias Pramasari, M.Si.
 Hikmah Hibatullah, S.E.
 Riska Surya Ningrum, M.Sc
Programme Coordinator:
 Wida Banar Kusumaningrum, M.T
 Lilik Astari, M.For.Ecosys.Sc
 Fenny Clara Ardiati, S.T.
1
 Dita Meisyara, S.Si.

 Kharisma Panji Ramadhan, S.Si.
 Resti Marlina, M.Si
 Prabu Satria Sejati, M.Si
 Putri Amanda, M.Si
Publication and Documentation:
 Fazhar Akbar
 Agung Sumarno, S.T., M.T.
 Eko Widodo, S.T.
 Oktan Dwi Nurhayat, M.Si
 Syam Budi Iryanto, A.Md.
Accomodation, Transportation and Logistic :
 Yusup Amin, M.Si.
 Ismadi, MT.
 Dimas Triwibowo, S.T.
Sponsorship:
 Dr. Dewi Sondari
 Adik Bahanawan, S.Hut.
 Kurnia Wiji Prasetiyo, S.Hut., M.Si.
Technical Support:
 Sudarmanto
 Anugerah Fajar, S.Si.
 Jayadi
 Teguh Darmawan ST.
 Deni Zulfiana, M.Si.
 Anis Sri Lestari, M.S.
2
Foreword
Welcome to HSS-ISSH 2019
By carrying out the theme “Integrated Smart Technology and Smart Society
for Sustainable Humanosphere“, Indonesian Institute of Sciences (LIPI) and
Research Institute for Sustainable Humanosphere (RISH) – Kyoto University
are proudly present The 9th International Symposium for Sustainable
Humanosphere (The 9th ISSH) that will be held at Grand Savero Hotel, Bogor,
Indonesia from 28 to 29 October 2019 which is an event of Humanosphere
Science School (HSS) 2019. On behalf of the organizing committee, we give
our hearty welcome to all participants of HSS-ISSH 2019.
Recently, forest science, bioscience, earth science, community-based

development and socioeconomic science, also applied science and
technology always be an interesting issues in our life, especially in sustainable
development goals. In the 9th ISSH 2019 symposium, we are going to provide
an opportunity to exchange knowledge, new ideas and advances in science
and technology for all participants.
We would like to thank JST/JICA for the support according to JASTIP and
SATREPS projects, as well as all the sponsors: PT. Ditek Jaya, PT. Infiniti
Bioanalitika Solusindao, Thermo Fisher, PT. Johnson Home Hygene Products,
PT. Perkindo Mitra Anal, PT. Unitama Multikarya Perkasa, PT. GeneCraf Labs,
PT. Petra Karunia Persada, PT. Biogen Scientific, and PT. Papua Hutan Lestari,
and surely to the organizing and technical committee members for their
tremendous efforts in preparing this conference. We hope this conference
will become a strong science and technology academic community for
worldwide researchers, academicians and professionals in fields of bio and
earth science, community-based development and socioeconomic science,
also applied science and technology.
Thank you
Organizing Committee Chair
Ikhsan Guswenrivo
3
Programme
Day 1,
Moday, 28 October 2019
07.30 – 08.30 Registration
08.30 – 08.35 Opening Ceremony
08.35 – 08.50 Opening remark from LIPI
Prof Dr Enny Sudarmonowati
08.50 – 09.05 Opening remark from RISH, Kyoto University
Prof. Takashi Watanabe
09.05 – 09.10 Photo session
Lecture session I (Earth Science)
09.10 – 09.40 Lecture 1
Dr. Tatsuhiro Yokoyama
(RISH, Kyoto University, Japan)
09.40 – 10.10 Lecture 2
Prof. Mitsuru Osaki, PhD
(Japan Peatland Society – JPS, Japan)
10.10 – 10.25 Coffe break
Lecture session II (Forest & Bioscience)
10.25 – 10.55 Lecture 3
Prof. Philippe Gerardin
(Universiti de Lorraine, France)
10.55 – 11.25 Dr. Hazwan Hussin
(Universiti Sains Malaysia, Malaysia)
11.25 – 12.00 Keynote speaker from Walikota of Bogor
Dr. Bima Arya
12.00 – 13.00 Lunch
13.00 – 14.00 Poster session (Flash talk)
Lecture session III (Satreps Project)
14.00 – 14.10 Brief introduction of Satreps Project
Prof. Toshiaki Umezawa
14.10 – 14.40 Lecture 5
Dr. Masaru Kobayashi
14.40 – 15.10 Lecture 6
Dr. Masahiro Sakamoto
4
(GSA, Kyoto University, Japan)

15.10 – 15.40 Lecture 7
Dr. Kenji Umemura
15.55 – 16.55 Paralel session I
16.55 – 17.00 Closing day 1
Day 2,
Tuesday, 29 October 2019
07.30 – 08.30 Registration
08.30 – 09.00 Keynote from Rector of IPB
Dr. Arif Satria,S.P., M.Si
Lecture session IV (Jastip Project)
09.00 – 09.10 Brief Introduction of Jastip project
Prof. Mamoru Kanzaki
(School of Agriculture, Kyoto University, Japan)
09.10 – 09.40 Lecture 8
Kuniyoshi Shimizu, Ph.D
(Faculty of Agriculture, Kyushu University,
Japan)
09.55 – 10.25 Lecture 9
Dr. Dede Heri Yuli Yanto
(Research Center of Biomaterials LIPI,
Indonesia)
10.25 – 10.55 Lecture 10
Prof. Andria Agusta
(Reseacrh Center of Biology LIPI, Indonesia)
Lecture session V
10.55 – 11.25 Lecture 11
Yunimar, S.Si, M.Si
(ICISFRI, Indonesia)
11.25 – 11.55 Lecture 12
Dr. Aya Yanagawa
11.55 -13.00 Lunch
Lecture session VI (E-Asia)
5
13.00 – 13.10 Brief Introduction of e-Asia Project

Prof. Takashi Watanabe
13.10 – 13.40 Lecture 13
Dr. Euis Hermiati
(Research Center of Biomaterials LIPI,
Indonesia)
13.40 – 14.10 Lecture 14
Dr. Pattanop Kanokratana
(Biotech, NSTDA)
14.10 – 14.40 Lecture 15
Dr. Chartchai Khanongnuch
(Faculty of Agro-Industry, Chiang Mai
University)
14.40 – 15.40 Paralel session III
15.55 – 16.55 Paralel session IV
Closing ceremony
17.00 – 17.05 Best presenter announcement
17.05 – 17.10 Closing remark from RISH, Kyoto University
17.10 – 17.15 Closing remark from LIPI
6
Oral Presentation
Monday, September 28th 2019 Session I: 15.55 – 16.55

Name Tittle Institution Page
Flamboyan Room (Earth Science)
Moderator: Dr. Nanang Masruchin
Eddy Vertically Propagating of Kelvin waves LAPAN1 130
Hermawan Near Tropopause Observed by the
Equatorial Atmospheric Radar (EAR)
Erma WRF Sensitivity to Simulate Early LAPAN1 131
Yulihastin Morning Precipitation in the Diurnal
Cycle over Coastal Region
Jefriza Application of Time-Series Sentinel-1A USM2 132
for Land Deformation in Central Aceh,
Indonesia
Nur Flood Inundation Mapping Using LAPAN1 133
febrianti Synthetic Aperture Radar Data Dual
Polarization: A Case Study of Flood in
Lake Tempe, South Sulawesi –
Indonesia
Muhammad Land Surface Temperature Estimation Unsyiah3 134
Rusdi Before the Aceh Tsunami (Case Study
of West Aceh)
Robi Calibration Of Rainfall Ensemble BMKG4 135
muharsyah Prediction Of ECMWF System 4 Using
Bayesian Model Averaging

Lavender Room (Bioscience)
Moderator: Dr. Maya Ismayati
Faustine Improving Cheese-making Process UI9 119
Sheryl with the combination of
Adeboi Transglutaminase and Papain
Enzyme
7
Tri Effect of Artocarpus heterophyllus USU6 108

Widyawati Leaf Ethanol Extract on Skin
Smoothness
Etti Satrina Antioxidant activity of liverworts USU6 127
Siregar Marchantia paleacea Bertol
Soh Li-Shen Comparison of Cuticular Thickness USM2 111
between Two Insecticide Resistant
Strains of Tropical Bed Bug, Cimex
hemipterus F. (Hemiptera: Cimicidae)
Dina Analysis of Passion Fruit Seed’s USU6 112
Keumala Sari Ethanol
Dina Vitamin D deficiency, vitamin A, beta USU6 113
Keumala Sari carotene and other parameter in
tuberculosis patients

Tulip Room (Bioscience)
Moderator: Dr. Titik Kartika
Tri Evaluation of Myrmecodia pendans USU6 109
Widyawati Water Extract on Blood Glucose Level
and Liver Function in Diabetic Patients
Farhat The Association of GSTM1 USU6 114
Polymorphism with IgA Level of
Nasopharyngeal Carcinoma Patients in
Gunungsitoli
Farhat The Association of GSTM1 USU6 115
Polymorphism with Total Antioxidant
Status of Nasopharyngeal Carcinoma
Patients in Gunungsitoli, Nias
Yeow Lit The Superiority of Chitosan in USM2 110
Chow Promoting Micropropagation of a
Dendrobium Hybrid Orchid
Iwan Histopathology of Lung and Trachea of USU6 117
Risnasari Mice (Mus Musculus) Exposed by
Formaldehyde Emissions from
8
Particleboards of Tea Leaf waste

(Camellia sinensis L.)
Farhat Polymorphism of CYP2E1 in USU6 116
nasopharyngeal carcinoma patients in
Haji Adam Malik General Hospital
Medan
Tuesday, September 29th 2019 Session II: 14.40 – 15.40

Rafflesia Ballroom (Applied Science and Technology)
Moderator: Dr. Firda Aulia Syamani
Putri Wulan Removal of Fe(II) from aqueous UGM8 145
solutions by chitosan activated
carbon composite study isoterm
and kinetic adsorption
Yufita Pyrolysis Of Palm Oil Coconut UGM8 146
Tuhuteru Biomass With Pig Iron Catalyst
Asful Hariyadi Characterization and Reduction UGM8 147
Behavior of Carbon-Infiltrated
Goethite Ore by Impregnation of
Tar Recovered from Coke Oven Gas
Kurnia Wiji Properties of polyninyl alcohol LIPI5 148
Prasetiyo composite filled Ampel bamboo
(Bambusa vulgaris) microfibrils
fibrillated by mechanical treatment
Bernadeta Ayu Selective Voltammetric Detection LIPI5 149
Widyaningrum of Dopamine Using Ferrocene
Modified Carbon Paste Electrode

Flamboyan Room
(Community-based development and socioeconomic science)
Moderator: Dr. Khoirul Himmi Setiawan
Arlinda Sari Effectiveness Of Promotion About USU6 142
Wahyuni Nutrition With A Lecture Method And
Leaflet Media On Knowledge And
9
Attitude Of The Mothers Who Have A

Child Under Five Years
Nisrina Socio-economic Analysis of LIPI5 139
Akmiyati Traditional Diamond Mining
Community in Banjarbaru City, South
Kalimantan Province
Najwa Dilemmas of New Rich People: Social LIPI5 140
Kahani Changes in Rural Communities
Fatima Affected by Kediri Airport
Development
Messalina L Partnership In Management Of UNB7 143
Salampessy Private Forest In Megamendung
Village, Bogor District
Aulia Rural Livelihood Strategy in Sumatra LIPI5 141
Afiifatuz After ‘the ASEAN 2020 Haze Free
Zulfah Policy’ Implementation

Lavender Room (Forest Science)
Moderator: Dr. Sasa Sofyan Munawar
Rahmawaty Mapping of Parkia speciosa land USU6 104
suitability distribution as one of
Multy Purpose Tree Species at
community agroforestry land
Didi Tarmadi Study on lignocellulose degradation LIPI5 103
by wood borer beetles,
Heterobostrychus aequalis and
Dinoderus minutus
Resti Community Forest for Increasing IPB10 105
Kharisma Transtition Zone (Suburban)
Environment: Water Infiltration and
Fresh Air
Tri Broto Development of Identification LIPI5 106
Siswoyo System for Limited Wood Species
10
Kartini The Relatioship of Dimension and IPB10 102

Ampel Bamboo Geometry
on Its Modulus of Elasticity
Tulip Room (Applied Science and Technology)
Moderator: Dr. Sukma Surya Kusumah
Wida Banar The Effectivity of Concentrated LIPI5 150
Kusumaningrum Maleic Acid for Nanocellulose
Isolation from Betung Bamboo
(Dendrocalamus asper sp) Pulp
Megawati Kinetics of soursop leaves UNNES11 151
antioxidant extraction through
microwave assisted extraction
technique
Sari Delviana The Performances of bark flour as IPB10 152
Marbun filler in Phenol Formaldehyde in
Glulam Manufacturing from
Benuang and Duabanga Wood
Sauria Karina Influence of Transglutaminase UI9 153
Enzyme Incorporated into
Protein based Edible Coating for
Preservation of Spanish
Mackerel Fish (Scomberomorus
commersoni)
Lilik Astari Characteristics of alkaline treated LIPI5 158
binderless particleboard made
from sorghum stalks
Session III: 15.55 – 16.55

Raflesia Ballroom (Applied Science and Technology)
Moderator: Dr. Sandi Sufiandi
Muhammad Development of Mobile-Based LIPI5 154
Rosyid Arifin Application for Practical Wood
Identification
11
Chici Performance Test Of Adsorption Of UGM8 155

Wardiani Nickel Ions With Porous Carbon
Prasongko From Mlanding Wood Which Has
Been Oxidized With Hydrogen
Peroxide
Aprilia Enhancement of Color, Dimensional IPB10 159
Kartikawati Stability, and Durability of Fast-
growing Teakwood by Mild Pyrolysis
Prabu Satria Modification of Rice Husk for Eco LIPI5 156
Sejati friendly Particleboard
Emi Yuliarita Resistance Characteristics of Natural LEMIGAS12 157
Rubber Compounds to Dimethyl
Ether (DME) and DME / LPG
Mixtures

Flamboyan Room (Bioscience)
Moderator: Dr. Didi Tarmadi
Widamayanti Isolation of an Erythromycin UI9 123
Resistant Gene from Bacillus
halodurans CM1
Mukmin Effect of Glucose, Sucrose, and UGM8 124
Sapto Lactose Solution on The Stability of
Pamungkas Betacyanin Pigment from Red
Dragon Fruit (Hylocereus polyrhizus)
Peels
Fitria Ningsih Optimization of laccase production UI9 125
from a newly isolated Trametes sp.
EDN134
Titik Kartika Insecticidal Activity of Clove LIPI5 126
(Syzigium aromaticum) and
Cinnamon (Cinnamon verum)
Essential Oils against Stored Product
Pest, Tribolium sp.
12
Riksfardini Development of Bamboo LIPI5 121

Annisa Propagation as Source of Bamboo-
Ermawar based Activated Carbon

Lavender Room (Bioscience)
Moderator: Dr. Dewi Sondari
Sulistiani Assessment of Potential Probiotic LIPI5 118
Lactic Acid Bacteria from Tempe and
Tape
Vallery Transformation and Co-expression of UI9 120
Athalia pPAMC125 and pBBRE194 prot-CM1
Priyanka in Several Strains of Escherichia coli
Farhah Ayu Preparation of Carbon Monolith UGM8 128
Fajrin Based Phenolic Resin and Its
Application for Antibiotic Adsorption
Yuni Phenotypic and Molecular Changes LIPI5 122
Wahyuni in Cassava (Manihot esculenta
Crantz.) Genotype as a Response to
Water-Deficit Stress
Maya Evaluation of antimicrobial pathogen LIPI5 160
Ismayati activity from Toona Sinensis

Tulip Room (Bioscience)
Moderator: Dr. Euis Hermiati
Ridho Selected of Potted Anthurium Clones IOCRI13 165
Kurniati through Hybridization
Khoirul Termite Biodiversity in an Urban LIPI5 163
Himmi Conservation Area, Cibinong Science
Setiawan Center-Botanical Garden, Bogor,
Indonesia
Nilam Lasiodiplodia theobromae as a new LIPI5 162
Fadmaulidha record from Enggano Island
Wulandari
13
Ikhsan The influences of social-community LIPI5 161

Guswenrivo behavior into ants-pest distribution
Apriwi Efficacy of Several LIPI5 164
Zulfitri Entomopathogenic Microorganism as
Microbial Insecticide Agains Insect
Pest on Chili (Capsicum annum L.)
NB:
1
National Institute of Aeronautics and Space
2
Universiti Sains Malaysia
3
Syiah Kuala University
4
Meteorological, Climatological and Geophysical Agency
5
Indonesian Institute of Sciences
6
University of North Sumatra
7
Nusa Bangsa University
8
Gadjah Mada University
9
University of Indonesia
10
IPB University
11
Semarang State University
12
Research and Development Centre for Oil and Gas Technology
13
Indonesian Ornamental Crops Research Institute
14
POSTER PRESENTATION
Code Name Title Page

Forest Science
P1 – 01 Agus Wahyudi Effect of Malic Acid Content and 168
Pressing Temperature on the
Performance Properties of Hybrid
Fiberboard from Mahang Wood
(Macaranga hypoleuca) and Bamboo
(Dendrocalamus asper)
P1 – 02 Dwi Ajias Effect of different extraction 169
Pramasari methods on starch yield extracted
from oil palm trunk (Elaeis
guineensis Jacq.)
P1 – 03 Ismail Budiman Physical and Mechanical Properties 170
of Five Indonesian Wood Treated
with Polystyrene
P1 – 04 Khalifah Insan Forest/Land Fire Smoke Detection 171
N. Rahmi Using GCOM-C Data
P1 – 05 Laode Alhamd Diversity of vegetation types in three 172
forest ecosystems, Mappi, Papua
P1 – 06 Mulyati Rahayu Diversity of Medicinal Plants In Yard 173
In Several Villages in Indonesia
P1 – 07 Sukma Surya Novel Application of Citric Acid 174
Kusumah Adhesive in The Manufacturing of
Eco-friendly Plywood Made from
Jabon (Anthochephalus cadamba)
P1 – 08 Siti Sundari Biodiversity study of several 175
peatland types in Papua
P1 – 09 Wahyu Cell Wall Thickness and Occupancy 176
Dwianto Measurements of Sumba Island
Wood Species
BIOSCIENCE
P2 – 10 Sri Characterization and effectiviness 178
Purwaningsih test of Rhizobium bacteria from
15
various plants on soybean (Glycine

max L) plants in greenhouse
P2 – 11 Suliasih Isolation Indole Acetic Acid (IAA) 179
producing Bacillus siamensis and
optimization of the physicochemical
conditions for IAA production
P2 – 12 Nuril Hidayati Assessment on Lead and Cadmium 180
Bioaccumulators for
Phytoremediation Contaminated
Rice Fields in Bekasi Districts, West
Java
P2 – 13 Atit Kanti Amylolytuc Yeast Schwanniomyces 181
alluvius, Candida glabrata and
Candida sp. for ethanol production
P2 – 14 Nunik Utilization Of Acetonitril And 182
Sulistinah Benzonitril As Substrates And
Identification Of Its Conversion
Products by rhodococcus
pyridinovorans strain i-benzo
Isolated From Leather Tanning
Waste, Garut
P2 – 15 Suciatmih Effect of Dyeing Repetition and Color 183
Reinforcement Materials on Cloth
Color by Mixed Fungi Dyes
P2 – 16 Yati Sudaryati Bacillus subtilis C2 Producing Lipase 184
Soeka Isolated from Bulk Shrimp Paste in
Samarinda East Kalimantan
P2 – 17 Yuliar Biocontrol of postharvest 185
Colletotrichum decay of red chilies
and tomatoes with Bacillus subtilis
ATCC 21556
P2 – 18 Rini Handayani Effects of Fermented Feed 186
Supplementation in Circulated
Aquaponic System with catfish
(Clarias sp.) on Growth of Lettuce
(Lactuca sativa L.)
16
P2 – 19 Sri Widawati Collaboration test of rhizobacteria as 187

biostimulant, vesicular arbuscular
mycorhizza (VAM) and graded dose
of NPK fertilizer on the growth of bok
choy (Brassica rapa L.)
P2 – 20 Deni Zulfiana Screening of Some Plant Extracts 188
from Lake Toba-North Sumatra for
controlling Wood-Rotting fungi
P2 – 21 Nabila Ayu Bioremediation of Phenanthrene 189
Budianti by Ocean Bacteria from the
Collection of Microbiology
Laboratory in LIPI Cibinong, Bogor
EARTH SCIENCE
P3 – 22 Risyanto Himawari-8 Rainfall Estimation from 191
Infrared Channels Based on Machine
Learning Methods
P3 – 23 Lely Qodrita A Comparative Analysis of the Wind 192

Avia and Wave on the Extreme Weather
Events in the Southern Sea of Java
P3 – 24 Nur Setiawan Impact of Radar Data Assimilation 193
on the Accuracy of Heavy Rain
Events Prediction in Bangka Island
(Case Study: Flood 8-9 February
2016)
P3 – 25 Ibnu Fathrio Evaluation of planetary boundary 194
layer schemes on Weather Research
Forecasting by using intensive
observation at Kototabang
P3 – 26 Heries Spatial Analysis of Correlation 195
Satyawardhana between Predicted Rainfall Based on
Dynamic Model and Satellite
P3 – 27 Iis Sofiati The Analysis of tropical cyclones 196
that occurred in the southern sea of
Java during the period 2004-2019
17
and their effects on sea-

atmospheric conditions
P3 – 28 Ina Juaeni Variability and sensitivity of stability 197
indices at the tropical region
Community-based Development and Socioeconomic Science
P4 – 29 Linar Humaira Relationship Between The 199
Socioeconomic Characteristics Of
Farmers And The Competency Level
Of Rice Seed Producers In Bogor
Regency
P4 – 30 Laily Estimating Ecosystem Services of 200
Mukaromah Urban Green Space in a Tourism
Landscape of Bali, Indonesia
P4 – 31 Arlinda Sari Interaction Quality among Doctor 201
Wahyuni and Hypertension Patiens in
Primary Health Care, in Medan,
Indonesia
Applied Science and Technology
P5 – 32 Firda Aulia Effect of Silica in Sembilang Bamboo 203
Syamani Skin on the Adhesion of Citric Acid
and Bamboo Particles
P5 – 33 Nanang Obtaining Different Charged 205
Masruchin Cellulose Nanofibrils by TEMPO-
mediated Oxidation
P5 – 34 Vilya Safarina The Utilization of Oil Palm Leaves 206
(Elaeis guineensis Jacq.) Waste as an
Antibacterial Solid Bar Soap
P5 – 35 Dewi Susan Phylogenetic Study of Some 207
Ganoderma from Indonesia: a
preliminary study
18
Keynotes
19
Dr. Arif Satria, SP, M.Si
Rector of IPB University Email : arifsatria@apps.ipb.ac.id

Jl. Raya Dramaga, Babakan
Bogor, Jawa Barat
16680
EDUCATION
 Doctoral Degree in Marine Policy, Kagoshima University, Japan

 Master Degree in Rural Sosiology, IPB University, Indonesia
 Bachelor Degree in Social Economy, IPB University, Indonesia
WORK EXPERIENCES
1996 – 1997 Secretary of Rector IPB

1997 – 2000 Executive Secretary of the Information Resources
Institute (LSI) IPB
1997 – 2000 The 2ndSecretary of the Socio-Economic Department of
Fisheries, Faculty of Fisheries –IPB
1999 – 2002 Head of the Coastal Community Agrarian Program,
Center for Agrarian Studies –IPB
2006 – 2008 Head of Division of Coastal Community Empowerment,
Center for Coastal and Ocean Studies –IPB
2007 – 2010 Secretary of Division of the Population, Agrarian
Studies and Political Ecology, Department of
Communication & Community Development –IPB
2008 – 2010 Director of Research and Strategic Studies –IPB
2010 – 2018 Dean of Faculty of Human Ecology –IPB
2017 – 2022 Rector of IPB
20
Dr. Bima Arya

Mayor of Bogor Email : bimasugiarto@gmail.com
Jl. Jatiluhur XII No.6 BSI
Bogor
Jawa Barat
EDUCATION
 Doctoral Degree in Political Science, Australian National University,

Australia (2006)
 Master of Arts, Monash University, Australia (1998)
 Bachelor Degree in International Relations, Parahyangan University,
Indonesia (1996)
WORK EXPERIENCES
1998 – 2001 Lecturer at Parahyangan University

2001 – 2014 Lecturer at Paramadina University
2001 – 2002 Staff of Vice-Rector at Paramadina University
2004 – 2006 Researcher at Research School for Pacific and
Asian Studies, Canberra
2006 – 2010 Executive Director of Lead Institute Paramadina
2007 – 2008 Consultant at Partnership for Governance Reform,
UNDP
2008 – 2010 Director Executive Charta Politika Indonesia
2009 – 2010 Editor in Chief of Rakyat Merdeka Magazine
2010 Lecturer at Graduate School of Paramadina
University
2010 Commissioner of Charta Politika Indonesia
2014 Mayor of Bogor
21
Lectures
22
Tatsuhiro Yokoyama
Research Institute for Sustainable Humanosphere Kyoto University
Gokasho, Uji, Kyoto 611-0011, Japan
Office Tel : +81 774 38 3810
Office Fax: +81 774 31 8463
Email : yokoyama@rish.kyoto-u.ac.jp
FIELD OF RESEARCH
Ionospheric physics, Numerical simulation
EDUCATION
2001 – 2004 Doctor of Informatics, Department of Communications
Computer Engineering, Graduate School of Informatics,
Kyoto University, Japan
1999 – 2001 Master of Informatics, Department of Communications
Computer Engineering, Graduate School of Informatics,
Kyoto University, Japan
1995 – 1999 Bachelor in Electrical and Electronic Engineering, Faculty of
Engineering, Kyoto University, Japan
PUBLICATION
23
2019 Yokoyama, T., H. Jin, H. Shinagawa, and H. Liu, Seeding of

equatorial plasma bubbles by vertical neutral wind, Geophys. Res.
2017 Lett., 46, 7088-7095, doi:10.1029/2019GL083629, 2019.
Yokoyama, T., A review on the numerical simulation of equatorial
plasma bubbles toward scintillation evaluation and forecasting,
2017 Prog. Earth Planet. Sci., 4, 37, doi:10.1186/s40645-017-0153-6,
2017.
Yokoyama, T., and C. Stolle, Low and midlatitude ionospheric
2015 plasma density irregularities and their effects on geomagnetic
field, Space Sci. Rev., 206, 495-519, doi:10.1007/s11214-016-
0295-7, 2017.
2014 Yokoyama, T., H. Jin, and H. Shinagawa, West wall structuring of
equatorial plasma bubbles simulated by three-dimensional HIRB
model, J. Geophys. Res. Space Physics, 120, 8810-8816,
2009 doi:10.1002/2015JA021799, 2015.
Yokoyama, T., H. Shinagawa, and H. Jin, Nonlinear growth,
bifurcation and pinching of equatorial plasma bubble simulated
2004 by three-dimensional high-resolution bubble model, J. Geophys.
Res. Space Physics, 119, 10,474-10,482,
doi:10.1002/2014JA020708, 2014.
Yokoyama, T., D. L. Hysell, Y. Otsuka, and M. Yamamoto, Three-
dimensional simulation of the coupled Perkins and Es layer
instabilities in the nighttime midlatitude ionosphere, J. Geophys.
Res., 114, A03308, doi:10.1029/2008JA013789, 2009.
Yokoyama, T., S. Fukao, and M. Yamamoto, Relationship of the
onset of equatorial F-region irregularities with the sunset
terminator observed with the Equatorial Atmosphere Radar,
Geophys. Res. Lett., 31, L24804, doi:10.1029/2004GL021529,
2004
24
Equatorial Ionospheric Disturbances and Its Social Impacts
Tatsuhiro Yokoyama
Research Institute for Sustainable Humanosphere, Kyoto University, Uji,
Japan
Abstract
The Earth’s upper atmosphere above 100 km altitude is partially

ionized by the solar radiation and called the ionosphere. Radiowave
propagation is influenced by plasma in the ionosphere and may cause
positioning and navigation errors or severe outage of satellite
communications. On the other hand, atmospheric gravity waves, which are
produced by topography, cumulus activity and others. propagate from the
lower layers of the Earth's atmosphere and have a significant influence on the
behavior of electromagnetic phenomena in the ionosphere. The ionosphere
absorbs a large amount of energy both from above and below. It is necessary
to understand physical and chemical processes in the ionosphere in order to
utilize precise positioning and navigation systems and stable satellite
communications.
Among various ionospheric phenomena, equatorial plasma bubble
(EPB) is a well-known phenomenon in the equatorial ionosphere. As it causes
severe scintillation in the amplitude and phase of radio signals, it is important
to understand and forecast the occurrence of EPB. The development of EPB
is known as a evolution of the generalized Rayleigh-Taylor instability. It has
been intensively studied by observations and numerical simulations. The
Equatorial Atmosphere Radar (EAR) in Kototabang, West Sumatra, Indonesia
is one of the largest observatories in the equatorial region. The EAR is
sensitive to 3-m scale ionospheric irregularities, which can be regarded as a
tracer of EPBs. The rapid beam steering capability of the active phased array
system of the EAR, along with simultaneous ground-based and satellite
observations, has revealed important aspects such as spatial/temporal
structures of EPBs.
Numerical simulations of the instability on the equatorial two-
dimensional plane have been conducted since the late 1970's, and the
nonlinear evolution of the instability has been clearly presented. Recently,
25
three-dimensional (3D) simulations became popular tools for further

understanding of the development of EPB such as 3D structure of EPB,
meridional wind effects and gravity wave seeding. A High-Resolution Bubble
(HIRB) model with a grid spacing of as small as 200 m has been developed and
provides a unique opportunity to characterize intermediate-scale EPB
structure, which was not well resolved until very recently. In the near future,
the HIRB model will be integrated into the whole atmosphere-ionosphere
coupled model, so that the HIRB model can be operated under realistic
conditions and forecast the occurrence of EPBs in advance.
Key words: equatorial ionosphere, space weather, plasma bubble,

Equatorial Atmosphere Radar (EAR), numerical simulation
26
Osaki Mitsuru
Research Faculty of Agriculture
Email: mosaki@chem.agr.hokudai.ac.jp
Hokkaido University
Kita-9, Nishi-9, Kitaku, Sapporo JAPAN
FIELD OF RESEARCH
Soil Science and Plant Nutrition
EDUCATION
1978-1981 Doctor of Science, Graduate School of Agriculture, Hokkaido

University, Japan
1976-1978 Master of Science, Graduate School of Agriculture, Hokkaido

University, Japan
1972-1976 Bachelor of Science, Faculty of Agriculture, Hokkaido

University, Japan
PUBLICATION
2019 SHA, ZM., WATANABE., CHU, Q., OKA, N., OSAKI, M., SHINANO
Takuro: A Reduced Phosphorus Application Rate Using a
Mycorrhizal Plant as the Preceding Crop Maintains Soybean Seeds’
Nutritional Quality. J. Agric. Food Chem. 67 (1), 32–42 (2019) DOI:
10.1021/acs.jafc.8b05288
2019 Huynh Vuong Thu Minh, Masaaki Kurasaki, Tran Van Ty, Dat Quoc
Tran, Kieu Ngoc Le, Ram Avtar, Md. Mostafizur Rahman, Mitsuru
Osaki: Effects of multi-dyke-protection systems on surface water
quality in the Vietnamese Mekong Delta. Water 2019, 11(5), 1010;
https://doi.org/10.3390/w11051010
2016
27
Lahiru S. Wijedasa, Susan E. Page, Christopher D. Evans, Mitsuru

Osaki: Time for responsible peatland agriculture, Science, 354, 562,
2016 2016 (DOI: 10.1126/science.aal1794)
Eds. by Mitsuru Osaki and Nobuyuki Tsuji: "Tropical Peatland

Ecosystems", Springer Japan, Springer Tokyo, Heidelberg, New
York, Dordrecht and London (2016) (ISBN 978-4-431-55680-0, ISBN
2014 978-4-431-55681-7 (eBook), DOI 10.1007/978-4-431-55681-7)
Lead Authors: Gusti Zakaria Anshari (Indonesia), Kenneth Byrne

(Ireland), Elke Hodson (Switzerland/USA), Hans Joosten
(EC/WI/FAO), J. Boone Kauffman (IUCN/UNESCO), Leif
Klemedtsson (Sweden), Tuija Elina Lapvetelainen† (Finland),
Christoph Mueller (Germany), Phillip O'Brien (Ireland) and Mitsuru
Osaki (Japan): CHAPTER 1 of "2013 SUPPLEMENT TO THE 2006
IPCC GUIDELINES FOR NATIONAL GREENHOUSE GAS INVENTORIES:
WETLANDS -Methodological Guidance on Lands with Wet and
Drained Soils, and Constructed Wetlands for Wastewater
Treatment-" eds. by Edited by Takahiko Hiraishi, Thelma Krug,
Kiyoto Tanabe, Nalin Srivastava, Baasansuren Jamsranjav, Maya
Fukuda and Tiffany Troxler, Published by the Intergovernmental
Panel on Climate Change (IPCC), 2014, ISBN 978-92-9169-139-5
28
An Innovated Tropical Peatland Management
OSAKI Mitsuru
Research Faculty of Agriculture, Hokkaido University
Sapporo 060-8589, Japan
Abstract
Long term research of tropical peatland by Hokkaido University have

been mainly done and doing in Central Kalimantan, Indonesia from 1997,
founded mainly by JSPS (Core University Project), JICA-JST (SATREPS project),
and JICA (IJ-REDD project), focusing Hydrology, Carbon Flux, Biodiversity
(Vegetation, Animal, Fishery, Insect, Microorganisms), Biomass Production,
Soil Science and Plant Nutrition, Peat Fire, Introduction of Satoyama System
(Integrated Farming System), Land Management System, and Social
Activation, and so on.
From these long-term researches, we found most important three
principals in tropical peatland ecosystem. The first principal in tropical
peatland is that tree biomass productivity (Dry matter/year/ha) in Tropical
Peatland is very high, and higher than that in Tropical Mineral-Land. The
second principle in tropical peatland is that high biomass productivity
achieves in high ground water table condition. The third principle is that
nutrients of trees are supplied by nitrogen fixation and nutrient cycling.
Thus, we concluded that Management System of Temperate/ Boreal
Peatland is unfortunately inadequate, irrelevant, inappropriate to apply to
Tropical Peatland Management. As a result, misunderstanding of this tropical
peatland ecosystem have been causing miserable disaster such as large CO2
Emission by peat degradation (Oxidation by Microorganisms) and peat fire,
by "Drain Cultivation Methods" introduced.
From observation and study in native peatland forest, it is concluded
that native forest can survive and more activate in high ground water table
condition, depending on aerial roots and mound roots, because these root
support to absorb oxygen for air and nutrients from litter and N2 fixing. As
oxygen solubility into water is extremely small, oxygen must be supplied from
land surface.
29
In conclusion, nutrients and oxygen should be applied from peatland

surface in high ground water level, of which cultivation method is called as
"AeroHydro Culture" (reference Figure). "AeroHydro Culture" method will be
explained more detail in this paper.
Key words: AeroHydro Culture, Ground Water Table, Land Surface

Management, Nitrogen Fixation, Nutrients Cycling
30
Philippe GERARDIN
Université de Lorraine
Laboratoire d’Etudes et de Recherche sur le
Matériau Bois (LERMaB), EA 4370
Faculté des Sciences et Technologies
BP 70239, 54506 Vandoeuvre-lès-Nancy Cedex
Tél : 03.83.68.48.40
Mél : philippe.gerardin@lermab.uhp-nancy.fr
Education
1990 - Ph.D. in Organic Chemistry, Université Henri Poincaré, Nancy 1
1997 - Habilitation à diriger des recherches, Université Henri Poincaré,
Nancy 1
Professional Position
December 1st 1991 Associate Professor, Université Henri Poincaré,
Nancy 1
October 1st 2001 Professor, Université Henri Poincaré, Nancy 1
September 1st 2008 Full Professor, Université Henri Poincaré, Nancy 1
January 1st 2009 Vice Director of LERMaB, Université Henri Poincaré, Nancy1
January 1st 2011 Director of LERMaB, Université Henri Poincaré, Nancy 1
January 1st 2012, Full Professor, Université de Lorraine
Academic activities
1997 / 2001 - Head of Chemistry Department

2001 Animation of wood chemistry and preservation activities of LERMAB
2002 Member of International Research Group on Wood Protection
2005 - Coordinator of Wood Fibres Energy Environment Specialty of Master
FAGE (M1 and M2 degree)
2007-2011 External examinator for Moi University (Eldoret, Kenya)
2010 Member of COST FP 0904 and 1006
2011-2012- Head of LERMAB (preparation of evaluation)
2013-2017 - Head of LERMAB (first term)
31
2012 member of lab of Excellence ARBRE

2007-2011 External examinator for Moi University (Eldoret, Kenya)
2018-2022 - Head of LERMAB (second term)
Reviewer for wood science and technology scientific media
Research interest
Key-word: Biodegradation - Biocides -Chemical modification - Extractives -

Heat Treatment Preservation - Natural durability - Wood chemistry
Production: 150 publications in International Peer Reviewed Journals (H-
index 27, 2537 citations, WOS / H-index 32, 3959 citations, Google Scholar),
supervisor of 34 Ph.D. students, 96 conferences in international congress, 3
book's chapters, 12 invited conferences, 14 international seminars
Research activities of my group are centred on wood chemistry and wood
protection in order to improve and promote wood and wood derived
products utilizations. In this context, we are particularly interested in the
development of friendly environmentally preservation methods based either
on new generation chemicals or formulations with low toxicity or on thermal
and chemical modifications of wood. The group is currently well recognized
in Europe through different COST actions for its expertise on the chemical
modifications occurring during wood thermo transformation and their effects
on wood properties. We are also interested on the reasons of natural
durability of wood developing researches activities in the field of chemistry
of extractives and fungal mechanisms involved in wood degradation in
relation with IAM Unit. Another aspect of our research concerns valorisation
of extractives aimed to improve competitively of wood industries. The
laboratory is equipped with all the facilities to perform chemistry, wood
treatments and wood chemical analyses using different chromatographic (GC
MS, LC MS/MS, HPLC, CI...) and spectroscopic methods (FTIR, NMR...). All this
expertise is valorised through numerous papers in international journals and
collaborations with national and international industrial and academic
partners.
32
State of the Art and future perspectives of Non Biocide Wood Preservation
Methods
Philippe GERARDIN
Université de Lorraine, Inra, EA 4370 USC 1445 LERMAB, Faculté des

Sciences et Technologies, BP 70239, 54506 Vandoeuvre-lès-Nancy Cedex,
France
[email: philippe.gerardin@univ-lorraine.fr]
Abstract
Increasing environmental pressures over the last few years have led to
important changes in industrialised countries in regard to sustainable
development and especially in the field of wood preservation. Conventional
wood preservation systems generally involve impregnation of broadly active
biocides into the wood. However, these techniques are under increasing
scrutiny with respect to their environmental impacts, which may limit their
applications in the future. This has led to new opportunities for the
development of “non-biocide” alternatives based on chemical or thermal
modification of wood. Numerous methods have been described in the
literature during the last century to modify wood. However, only a few of
them have led to industrial development. The aim of the conference will be
to make in a first time an assessment of the main thermal and chemical
modifications of wood, which have currently find applications on industrial
scale, highlighting their main advantages and drawbacks and in a second time
to present some research results, which could constitute in the near future,
opportunities for development from an industrial point of view.
Key words : chemical modification, durability, biocide, thermal modification,

treatment, wood
33
Mohd Hazwan Hussin
School of Chemical Sciences Office Tel : +604 653 6378

Universiti Sains Malaysia Office Fax : +604 657 4854
11800 Minden, Penang, Home Tel : +6011 243 24 820
Malaysia Email : mhh@usm.my
FIELD OF RESEARCH
Wood Chemistry; Adhesives and Coatings; Corrosion Protection
EDUCATION
2011 – 2014 Doctor of Wood Science and Fiber, Faculte des Science et
Techniques, Universite de Lorraine, France
2008 – 2011 Master of Chemistry, School of Chemical Sciences, Universiti
Sains Malaysia, Malaysia
2002 – 2006 Bachelor in Chemistry, School of Chemical Sciences, Universiti
Sains Malaysia, Malaysia
PUBLICATION
2019
1) Evaluation of Piper sarmentosum extract’s corrosion inhibitive effects
and adsorption characteristics for the corrosion protection of mild steel
in 0.5 M HCl, (Nurul Adilla Rozuli, Tuan Sherwyn Hamidon and M.
Hazwan Hussin), Materials Research Express 6 (2019), 106524, IF: 1.449,
IOPScience
2) Characterisation and Kinetic Studies on Activated Carbon Derived from
Rubber Seed Shell for the Removal of Methylene Blue in Aqueous
Solutions, (Nur Fatin Silmi Mohd Azani, Caryn Tan Hui Chuin, Nor Salmi
Abdullah, Syazrin Syima Sharifuddin and M. Hazwan Hussin), Journal of
Physical Science 30(2) (2019), 1-20
3) Anticorrosive performance of AA6061 aluminium alloy treated with sol-
gel coatings doped with mangrove bark tannins in 3.5 wt% NaCl, (Tuan
34
Sherwyn Hamidon, Teoh Zhi Qiang, M. Hazwan Hussin), Materials

Research Express 6 (2019), 096417, IF: 1.449, IOPScience.
4) Physicochemical studies of tamarind shell tannins as a potential green
rust converter, (Abdullahi Abdulmajid, Tuan Sherwyn Hamidon, Afidah
Abdul Rahim, M. Hazwan Hussin), BioResources 14(3) (2019), 6863-
6882, IF: 1.334, NCSU.
5) Characterization and phytochemical studies of Dryobalanops aromatica
bark extract as a green rust converter, (Saraya A. Yakubu, M. Hazwan
Hussin, Afidah Abdul Rahim, Mohamad Nurul Azmi, Muhammad Hafiz
Husna Hasnan, Khalijah Awang), Journal of Corrosion Science and
Engineering (2019), Vol 22, (Preprint 36), 1-18.
6) Hydroxymethylation-Modified Lignin and Its Effectiveness as a Filler in
Rubber Composites, (Nor Anizah Mohamad Aini, Nadras Othman, M.
Hazwan Hussin, Kannika Sahakaro, Nabil Hayeemasae), Processes 7
(2019), 315, IF: 1.279, MDPI.
7) Electrochemically modulated liquid-liquid extraction for sample
enrichment, (Maizatul Najwa Jajuli, Mohd Hazwan Hussin, Bahruddin
Saad, Afidah Abdul Rahim, Marc Hebrant, Gregoire Herzog), Analytical
Chemistry (2019), IF: 6.042, ACS. Acctepted.
8) Application of K-impregnated staghorn coral as catalyst in the
transesterification of waste cooking oil, (Nabilah Atiqah Zul, Shangeetha
Ganesan, M. Hazwan Hussin), Sains Malaysiana 48 (2019), 803-811, IF:
0.565, Penerbit UKM.
9) The structural characterization and antioxidant properties of oil palm
fronds lignin incorporated with p-hydroxyacetophenone, (Nur Hanis
Abd Latif, Afidah Abdul Rahim, Nicolas Brosse, M. Hazwan Hussin),
International Journal of Biological Macromolecules 130 (2019), 947-957,
IF: 3.671, Elsevier.
10) Room-temperature synthesis of Bi/BiOBr composites for the catalytic
degradation of ciprofloxacin using indoor fluorescent light illumination,
(Saifullahi Shehu Imam, Rohana Adnan, Noor Haida Mohd Kaus, M.
Hazwan Hussin), Journal of Materials Science: Materials in Electronics
(2019), pp. 1-14, https://doi.org/10.1007/s10854-019-00930-z, IF:
2.019, Springer.
11) Potentials of Canarium schweinfurthii seed shell as a novel precursor for
CH3COOK activated carbon: statistical optimization, equilibrium and
35
kinetic studies, (Zaharaddeen N. Garba, M. Hazwan Hussin, Ahmad

Galadima, Ibrahim Lawan), Applied Water Science 9 (2019), 31, Springer.
12) Optimizing the acid hydrolysis process for the isolation of
microcrystalline cellulose from oil palm empty fruit bunches using
response surface methods, (Tuan Muhamad Hassan, Md Sohrab
Hossain, Mohamad Haafiz Mohamad Kassim, Mazlan Ibrahim, Nurul
Fazita Mohammad Rawi, M. Hazwan Hussin), Waste and Biomass
Valorization (2019), 1-16, IF: 1.874, Springer
13) Electrochemical data of single and hybrid sol-gel coating precursors for
aluminum alloy corrosion protection in 3.5% NaCl, (M. Hazwan Hussin),
Data in Brief 22 (2019), 971-976, Elsevier.
14) Physicochemical studies of kenaf nanocrystaline cellulose and poly (3-
hydroxybutyrate-co-3-hydroxyhexanoate) as filler for lithium
perchlorate based polymer electrolyte, (M. Hazwan Hussin, Nur Ain
Tajudin, Nur Fatin Silmi Mohd Azani, Murugan Paramasivam, M. K.
Mohamad Haafiz, Sudesh Kumar, Mehdi Yemloul), International Journal
of Electrochemical Sciences, 14 (2019), 1620 – 1633, IF: 1.469.
15) Biosynthesis of poly(3-hydroxybutyrate) (PHB) by Cupriavidus necator
from various pretreated molasses as carbon source, (Khok Yong Sen, M.
Hazwan Hussin, Siti Baidurah), Biocatalysis and Agricultural
Biotechnology 17 (2019), 51-59, Elsevier.
16) Development and characterization novel bio-adhesive for wood using
kenaf core (Hibiscus cannabinus) lignin and glyoxal, (M. Hazwan Hussin,
Alia Abdul Aziz, Anwar Iqbal, Mohamad Nasir Mohamad Ibrahim, Nur
Hanis Abd Latif), International Journal of Biological Macromolecules 122
(2019), 713-722, IF: 3.671, Elsevier.
2018
1) Study on adsorption of mercury from aqueous solution on activated
carbons prepared from palm kernel shell, (Nor Salmi Abdullah, Syazrin
Syima Sharifuddin, M. Hazwan Hussin), Key Engineering Materials 783
(2018), 109-114, Trans Tech Publications.
2) Development of Immobilized Microcrystalline Cellulose as an Effective
Adsorbent for Methylene Blue Dye Removal, (Caryn Tan Hui Chuin,
Sumiyyah Sabar, M. Hazwan Hussin), South African Journal of Chemical
Engineering 26 (2018), 11-24, Elsevier.
36
3) Condensed tannins from mangrove and grape pomace as renewable

corrosion inhibitors and wood adhesive, (François Gambier, Affaizza
Mohd Shah, M. Hazwan Hussin, Mohamad Nasir Mohamad Ibrahim,
Afidah Abdul Rahim, Nicolas Brosse), Journal of Advanced Chemical
Engineering 8 (2018), 1-8, IF: 1.43, OMICS International.
4) Production of oil palm (Elaeis guineensis) fronds lignin-derived non-toxic
aldehyde for eco-friendly wood adhesive, (M. Hazwan Hussin, Noraini
Abdul Samad, Nur Hanis Abd. Latif, Nurul Adilla Rozuli, Siti Baidurah
Yusoff, François Gambier, Nicolas Brosse), International Journal of
Biological Macromolecules 113 (2018), 1266-1272, IF: 3.671, Elsevier.
5) Isolation of microcrystalline cellulose (MCC) from oil palm frond as
potential natural filler for PVA-LiClO4 polymer electrolyte, (M. Hazwan
Hussin, Nurhanina Ayu Husin, Ibrahim Bello, Nurmaizatulhana Othman,
Mohamad Abu Bakar, M.K. Mohamad Haafiz), International Journal of
Electrochemical Sciences, 13 (2018), 3356 – 3371, IF: 1.469.
2017
1) Depolymerized oil palm frond (OPF) lignin products as corrosion
inhibitors for mild steel in 1 M HCl, (Affaizza Mohamad Shah, Afidah
Abdul Rahim, Mohamad Nasir Mohamad Ibrahim and Mohd. Hazwan
Hussin), International Journal of Electrochemical Sciences, 12 (2017),
9017 – 9039, IF: 1.469.
2) Preparation of environmental friendly phenol-formaldehyde wood
adhesive modified with kenaf lignin, (M. Hazwan Hussin, Hau Han
Zhang, Nurul Azila Aziz, Noraini Abd Samad, Abbas Hassan Faris,
Mohamad Nasir Mohamad Ibrahim, Anwar Iqbal, Ahmad Faiz Abdul
Latip, M.K. Mohamad Haafiz), Beni Suef University Journal of Basic and
Applied Sciences 6 (2017), 409-418, Elsevier.
3) Modification of oil palm fronds lignin by incorporation of m-cresol for
improving structural and antioxidant properties, (Nurul Atiqah Sa’don,
Afidah Abdul Rahim, Mohamad Nasir Mohamad Ibrahim, Nicolas Brosse,
M. Hazwan Hussin), International Journal of Biological Macromolecules
104 (2017), 251-260. IF: 3.671, Elsevier.
4) Preparation and characterization of activated carbon from moringa
oleifera seed pod, (Nor Salmi Abdullah, Mohd Hazwan Hussin, Syazrin
37
Syima Sharifuddin, Muhammad Azroie Mohamed Yusoff), Science

International 29 (2017), 7-11.
5) The effect of p-nitrophenol toward the structural characteristics
andantioxidant activity of oil palm fronds (OPF) lignin polymers, (Nurul
Atiqah Sa’don, Afidah Abdul Rahim, M. Hazwan Hussin), International
Journal of Biological Macromolecules 98 (2017), 701-708. IF: 3.671,
Elsevier.
6) Recent progress in cellulose nanocrystals: sources and production,
(Djalal Trache, M. Hazwan Hussin, M. K. Mohamad Haafiz, Vijay Kumar
Thakur), Nanoscale (2017) 9, 1763 – 1786, IF: 7.367, RSC.
7) Investigation of oil palm based Kraft and auto-catalyzed organosolv
lignin susceptibility as a green wood adhesives, (Abbas Hasan Faris,
Afidah Abdul Rahim, M. Hazwan Hussin, Mohamad Nasir Mohamad
Ibrahim, Adel Alkurdi, Ali Salehabadi), International Journal of Adhesion
and Adhesives 74 (2017), 115-122. IF: 2.211, Elsevier.
8) Influence of alkaline hydrogen peroxide pre-hydrolysis on theisolation
of microcrystalline cellulose from oil palm fronds, (Abdulwahab F.
Owolabi, M.K. Mohamad Haafiz, Md. Sohrab Hossain, M. Hazwan
Hussin, M.R. Nurul Fazita), International Journal of Biological
Macromolecules 97 (2017), 1228-1234. IF: 3.671, Elsevier.
38
Production Of Lignin-Derived Non-Toxic Aldehyde For Eco-Friendly Wood

Adhesive
M. Hazwan Hussin1) and Nicolas Brosse2)

1)
Materials Technology Research Group (MaTReC), School of Chemical
Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
2)
Laboratoire d'Etude et de Recherche sur le MAteriau Bois (LERMAB),
Faculte des Sciences et Techniques, Universite de Lorraine, Bld des
Aiguillettes, F-54500 Vandoeuvre-les-Nancy, France
Abstract
Lignocellulosic materials can significantly contribute to the

development of eco-friendly wood adhesives. In this work, glyoxal-phenolic
resins for plywood were prepared using organosolv lignin,whichwas isolated
from black liquor recovered fromorganosolv pulping of oil palmfronds (OPF)
and considered to be an alternative to phenol. Glyoxal,which is a dialdehyde
obtained from several natural resources,was used as substitute for
formaldehyde. The structure of organosolv lignin and the resins were
characterized by FTIR and NMR, and for thermal stability by TGA and DSC. The
resins were further studied for their viscosity, pH, solids content and gel
times. The resins performance as wood adhesive was further established
from mechanical test in terms of tensile strength and modulus of elasticity
(MOE) to obtain the optimum ratios of organosolv lignin, which replaces
phenol in organosolv lignin phenol glyoxal (OLPG) resins. The adhesive
composition having 50% (w/w) of phenol substituted by organosolv lignin,
termed as 50% OLPG showed highest adhesive strength compared to phenol
formaldehyde (PF) commercial adhesive.
Key words: Lignocellulosic materials, organosolv lignin, glyoxal, wood

adhesive
39
Toshiaki Umezawa
Research Institute for Sustainable Humanosphere
Kyoto UniversityGokasho, Uji, Kyoto 611-0011 Japan
Office Tel : +81-774 38-3625
Office Fax : +81-774 38-3682
Email: tumezawa@rish.kyoto-u.ac.jp
FIELD OF RESEARCH
Plant metabolic science and organic chemistry
EDUCATION
1976 – 1980 Department of Wood Science and Technology,
Faculty of Agriculture, Kyoto University, Japan
Awarded the degree of BSc in wood science and technology
1980 – 1982 Research Section of Lignin Chemistry,
Wood Research Institute, Kyoto University, Japan
Awarded the degree of MSc in wood science and technology
1987 Awarded the degree of PhD in Wood Science and Technology
(Ronpaku)
PUBLICATION
2019
Takeda Y, Tobimatsu Y, Yamamura M, Takano T, Sakamoto M,
Umezawa T, “Comparative evaluations of lignocellulose reactivity
and usability in transgenic rice plants with altered lignin
2019
composition”, J. Wood Sci. 65, 6
Takeda Y, Suzuki S, Tobimatsu S, Osakabe K, Osakabe Y, Ragamustari
SK, Sakamoto M, Umezawa T, “Lignin characterization of rice
2019
CONIFERALDEHYDE 5-HYDROXYLASE loss-of-function mutants
generated with the CRISPR/Cas9 system”, Plant Journal, 97, 543–554
Miyamoto T, Takada R, Tobimatsu Y, Takeda Y, Suzuki S, Yamamura
2019
M, Osakabe K, Osakabe Y, Sakamoto M, Umezawa T, “OsMYB108
loss-of-function enriches p-coumaroylated and tricin lignin units in
2018
rice cell walls”, Plant Journal, 98, 975-987
40
2018 Umezawa T, Yamamura M, Ono E, Shiraishi A, Ragamustari SK,

“Recent advances in lignan OMT studies”, Mokuzai Gakkaishi, 65, 1-
12
Umezawa T, “Lignin modification in planta for valorization”,
2018 Phytochem. Rev., 17, 1305-1327
Takeda Y, Tobimatsu Y, Karlen SD, Koshiba T, Suzuki S, Yamamura M,
Murakami S, Mukai M, Hattori T, Osakabe K, Ralph J, Sakamoto M,
Umezawa T, “Downregulation of p-COUMAROYL ESTER 3-
2018 HYDROXYLASE in rice leads to altered cell wall structures and
improves biomass saccharification”, Plant J., 95, 796-811
Miyamoto T, Mihashi A, Yamamura M, Tobimatsu Y, Suzuki S,
Takada R, Kobayashi Y, Umezawa T, “Comparative analysis of lignin
2017 chemical structures of sugarcane bagasse pretreated by alkaline,
hydrothermal, and dilute sulfuric acid methods”, Industr. Crops
Prod., 121, 124-131
2017 Miyamoto T, Yamamura M, Tobimatsu Y, Suzuki S, Kojima K, Takabe
K, Terajima Y, Mihashi A, Kobayashi Y, Umezawa T, “A comparative
study of the biomass properties of Erianthus and sugarcane:
2014 lignocellulose structure, alkaline delignification rate, and enzymatic
saccharification efficiency”, Biosci. Biotechnol. Biochem., 82, 1143-
1152
2014 Koshiba T, Yamamoto N, Tobimatsu Y, Yamamura M, Suzuki S,
Hattori T, Mukai M, Noda S, Shibata D, Sakamoto M, Umezawa T,
“MYB-mediated upregulation of lignin biosynthesis in Oryza sativa
2013 towards biomass refinery”, Plant Biotechnol., 34, 7-15
Takeda Y, Koshiba T, Tobimatsu Y, Suzuki S, Murakami S, Yamamura M,
Rahman MM, Takano T, Hattori T, Sakamoto M, Umezawa T, “Regulation
2013 of CONIFERALDEHYDE 5-HYDROXYLASE expression to modulate cell wall
lignin structure in rice”, Planta, 246, 337-349
Ragamustari SK, Yamamura M, Ono E, Hattori T, Suzuki S, Suzuki H,
2013 Shibata D, Umezawa T, “Substrate-enantiomer selectivity of
matairesinol O-methyltransferases”, Plant Biotechnol., 31, 257-267
Nakatsubo T, Ragamustari SK, Hattori T, Ono E, Yamamura M, Li L,
2013 Chiang VL, Umezawa T, “A new O-methyltransferase for monolignol
synthesis in Carthamus tinctorius”, Plant Biotechnol., 31, 545-553
41
2013 Ragamustari SK, Nakatsubo T, Hattori T, Ono E, Kitamura Y, Suzuki S,

Yamamura M, Umezawa T, “A novel O-methyltransferase involved in
the first methylation step of yatein biosynthesis in Anthriscus
sylvestris”, Plant Biotechnol., 30, 315-326
2010 Koshiba T, Murakami S, Hattori T, Mukai M, Takahashi A, Miyao A,
2010 Hirochika H, Suzuki S, Sakamoto M, Umezawa T, “CAD2 deficiency
causes both brown midrib and gold hull and internode phenotypes in
Oryza sativa L. cv. Nipponbare”, Plant Biotechnol., 30, 365-373
2008 Umezawa T, Ragamustari SK, Nakatsubo T, Wada S, Li L, Yamamura
M, Sakakibara N, Hattori T, Suzuki S, Chiang VL, “A lignan O-
methyltransferase catalyzing the regioselective methylation of
2007 matairesinol in Carthamus tinctorius”, Plant Biotechnol., 30, 97-109
Koshiba T, Hirose N, Mukai M, Yamamura M, Hattori T, Suzuki S,
Sakamoto M, Umezawa T, “Characterization of 5-
2003 Hydroxyconiferaldehyde O-Methyltransferase in Oryza sativa”, Plant
Biotechnol., 30, 157-167
Yamamura M, Noda S, Hattori T, Shino A, Kikuchi J, Takabe K, Tagane
S, Gau M, Uwatoko N, Mii M, Suzuki S, Shibata D, Umezawa T,
“Characterization of Erianthus arundinaceus lignocellulose in
relation to enzymatic saccharification efficiency”, Plant Biotechnol.,
30, 25-35
Umezawa T, “The cinnamate/monolignol pathway”, Phytochem.
Rev., 9, 1-17
Yamamura M, Suzuki S, Hattori T, Umezawa T, “Subunit composition
of hinokiresinol synthase controls enantiomeric selectivity in
hinokiresinol formation”, Org. Biomol. Chem., 8, 1106-1110
Nakatsubo T, Mizutani M, Suzuki S, Hattori T, Umezawa T,
“Characterization of Arabidopsis thaliana Pinoresinol Reductase, a
new type of enzyme involved in lignan biosynthesis”, J. Biol. Chem.,
283, 15550-15557
Suzuki S, Yamamura M, Hattori T, Nakatsubo T, Umezawa T, “The
subunit composition of hinokirsinol synthase controls geometrical
selectivity in norlignan formation”, Proc. Natl. Acad. Sci. USA, 104,
21008-21013
Umezawa T, “Diversity in lignan biosynthesis”, Phytochem. Rev., 2,
371-390
42
Brief Introduction about SATREPS Project

Producing Biomass Energy and Material through Revegetation of
Alang-alang (Imperata cylindrica) Fields
Toshiaki Umezawa1),2)
1)
Research Institute for Sustainable Humanosphere, Kyoto University
2)
Research Unit for Development of Global Sustainability
Abstract
Imperata cylindrica that is a typical invasive grass weed grows
extremely well on poor soil and has spread widely in tropical and subtropical
regions all over the globe including Indonesia. The conversion of Imperata
grasslands into biomass crop farmland would be valuable for renewable
resource production in a global context, which would contribute to the
welfare and economic improvement of Indonesian local communities and
therefore may provide long-term sustainable economic benefits to the
nation. In addition, the conversion of Imperata grasslands to biomass crop
farmland and/or plantation forests may lead to restoration of biodiversity. In
this context, the Research Institute for Sustainable Humanosphere and
Graduate School of Agriculture, Kyoto University, together with the
Indonesian Institute of Sciences are conducting “the Project for Producing
Biomass Energy and Material through Revegetation of Alang-alang (Imperata
cylindrica) fields” of SATREPS project supported by JICA (Japan International
Cooperation Agency) and JST (Japan Science and Technology Agency). This
project is in line with SDGs (Sustainable Development Goals) and of great
importance for a sustainable development path of the world in the era of
bioeconomy. In this project, we also put emphasis on capacity development
of young scientists and students of both countries. This session is one of our
capacity development activities and provides Indonesian young scientists the
fundamental knowledge in the scientific fields related to the SATREPS project,
including plant biotechnology, plant molecular biology, plant organic
chemistry, material science, plant nutrition and soil science.
43
Key words : Imperata cylindrica, alang-alang, SDGs, biomass energy,

biomaterial, revegetation
Masaru Kobayashi
Graduate School of Agriculture Office Tel : +81-75 753-6407

Kyoto University Office Fax : +81-75 753-6107
Kyoto 606-8502
Japan
Email: kobayashi.masaru.8e@kyoto-u.ac.jp
FIELD OF RESEARCH
Plant Nutrition
EDUCATION
2000 Doctor of Agricultural Sciences, Kyoto University, Japan
1994 – 1995 Doctoral course, Division of Agricultural Chemistry,
Graduate School of Agriculture, Kyoto University, Japan
1992 – 1994 Master of Agricultural Sciences, Division of Agricultural
Chemistry,
1988 – 1992 Bachelor in Agricultural Sciences, Department of
Agricultural Chemistry,
Faculty of Agriculture, Kyoto University, Japan
PUBLICATION
2018 Zhou Y, Kobayashi M, Awano T, Matoh T, Takabe K, “A new

monoclonal antibody against rhamnogalacturonan II and its
application to immunocytochemical detection of rhamnogalacturonan
2018 II in Arabidopsis roots”. Biosci. Biotech. Biochem. 82(10): 1780-1789
Kitajima S, Aoki W, Shibata D, Nakajima D, Sakurai N, Yazaki K,
Munakata R, Taira T, Kobayashi M, Aburaya D, Savadogo EH, Hibino S,
Yano H, “Comparative multi-omics analysis reveals diverse latex-based
44
2017 defense strategies against pests among latex-producing organs of the

fig tree (Ficus carica)” Planta 246(6): 1423-1438
Kobayashi M, Miyamoto M, Matoh T, Kitajima S, Hanano S, Sumerta
2017 IN, Narise T, Suzuki H, Sakurai N, Shibata D, “Mechanism underlying
rapid responses to boron deprivation in Arabidopsis roots”. Soil Sci.
Plant Nutr. 64: 106–115.
2013 Zhou Y, Awano T, Kobayashi M, Matoh T, Takabe K,
“Immunocytochemical detection of rhamnogalacturonan II on forming
2013 cell plates in cultured tobacco cells”. Biosci. Biotech. Biochem. 81:
899–905.
Oiwa Y, Kitayama K, Kobayashi M, Matoh T, “Boron deprivation
immediately causes cell death in growing roots of Arabidopsis thaliana
(L.) Heynh. Soil Sci. Plant Nutr. 59: 621–627.
Koshiba T, Kobayashi M, Matsuoka K, Fujiwara T, Matoh T, “Boron
nutrition of cultured tobacco BY-2 cells. VII. Rapid induction of
metabolic dysfunction in cells deprived of boron as revealed by
microarray analysis”. Soil Sci. Plant Nutr. 59: 189–194.
45
Development of new techniques for nutrition diagnosis of crop plants
Masaru Kobayashi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto
University,
Sakyo, Kyoto 606-8502, Japan
kobayashi.msaru.8e@kyoto-u.ac.jp
Abstract
Plants grow utilizing sunlight and minerals taken up from their

surroundings. They require the minerals containing 17 essential elements, of
which 14 except for hydrogen, carbon and oxygen are taken up by roots from
the soil. It is often the case that one of those essential elements are contained
in soil at insufficient amounts or in a form unavailable for absorption, thereby
inducing the deficiency of the element. To avoid such damage, fertilizer
application is a common practice in crop production. On the other hand,
excess amount of nutrients can also cause problems including toxicity or
environmental deterioration. Therefore, it is critical to apply necessary and
sufficient amount of fertilizers, based on the diagnosis of sufficiency with the
nutrients.
Conventional methods for nutrition diagnosis rely on visual
symptoms and/or tissue content of the elements. However, changes in these
parameters may become apparent only after the deficiency gets severe, thus
early detection and treatment of the problem may not be easy with these
methods alone. Recently, monitoring of plant physiological responses such as
gene expression has emerged as possible new methods for nutrition
diagnosis. As the change in gene expression is one of earliest responses to
environmental changes, the method has been considered promising as the
way to detect nutritional disorders before the visual symptoms arise. In this
lecture, I will introduce our trial to adopt the method for calcium nutrition
diagnosis, which would be important to control crop quality and stress
tolerance.
Key words : calcium, essential element, gene expression analysis,

nutrition diagnosis
46
Masahiro Sakamoto
Graduate School of Agriculture, Office Tel : +81-75 753-6464

Kyoto University Office Fax : +81-75 753-6471
Kitashirakawa Oiwake-cho, Sakyo-ku, Home Tel : +81-80 3039-7484
606-8502 Kyoto, JAPAN
Email: sakamoto.masahiro.6r@ kyoto-u.ac.jp
FIELD OF RESEARCH
Plant Molecular Biology
EDUCATION
2007 Doctor of Agricultural Science, Division of Forest and
Biomaterials Science,
1982 – 1984 Master of Agricultural Science, Division of Wood Science and

Technology,
1978 – 1982 Bachelor in Wood Science and Technology, Department of

Agriculture, Kyoto University, Kyoto
PUBLICATION
2019 Takuji Miyamoto, Rie Takada, Yuki Tobimatsu, Yuri Takeda, Shiro
Suzuki, Masaomi Yamamura, Keishi Osakabe, Yuriko Osakabe,
Masahiro Sakamoto and Toshiyuki Umezawa. 2019. OsMYB108
loss-of-function enriches p-coumaroylated and tricin lignin units in
2018 rice cell walls. Plant Journal. 98: 975 - 987. DOI:10.1111/tpj.14290
Yuri Takeda, Yuki Tobimatsu, Steven D Karlen, Taichi Koshiba, Shiro
Suzuki, Masaomi Yamamura, Shinya Murakami, Mai Mukai,
Takefumi Hattori, Keishi Osakabe, John Ralph, Masahiro Sakamoto
47
and Toshiaki Umezawa. 2018. Downregulation of p- COUMAROYL

2017 ESTER 3-HYDROXYLASE in rice leads to altered cell wall structures
and improves biomass saccharification. Plant Journal. 95:796 - 811.
DOI:10.1111/tpj.13988
Yuri Takeda, Taichi Koshia, Yuki Tobimatsu, Shiro Suzuki, Shinya
Murakami, Masaomi Yamamura, MdM Rahaman, Toshiyuki
2015 Takanao, Takefumi Hattori, Mashiro Sakamoto and Toshiyuki
Umezawa. 2018. Regulation of CONIFERALDEHYDE 5-
HYDROXYLASE expression to modulate cell wall lignin structure in
2013 rice. Planta 245:337 - 251. DOI:10.1007/s00425-017-2692-x
Tuyoshi Inoue, A Yoshinaga, Keiji Takabe, K Ogawa, Masahiro
Sakamoto, J Azuma and Yoichi Honda. In Situ Detection and
Identification of Hesperidin Crystals in Satsuma Mandarin (Citrus
unshuu) Peel Cell. Phytochemical Analysis 26:105-110.
DOI:10.1111/pca.2541
Xinten Lao, Junichi Azuma and Masahiro Sakamoto. Two cytosolic
aldolase show different expression patterns during shoot
elongation in Moso bamboo, Phyllostachys pubesencs Mazel.
Physiologia Plantrum 149: 422-431. DOI:10.1111/ppl.12052
48
The role of hemicellulose in the cell wall
Masahiro Sakamoto1)
1)
Graduate School of Agriculture, Kyoto University
Kitashirakawa Oiwake-cho, Sakyo-ku, 606-8502 Kyoto, Japan
Abstract
Plant cell wall is consisted with three major components; cellulose,

hemicellulose and lignin. Cellulose and hemicellulose cross-link via hydrogen
bond, and hemicellulose binds lignin with ester bonding though ferulic and
diferulic acids. In monocot plants, hemicellulose is mainly consisted with
arabinoxylan, which is xylan backbone with arabinofuranose and glucuronic
acid side chain. Then, hemicellulose binds cellulose and lignin, it is said that
hemicellulose is the adhesion of cell wall. However, hemicellulose content in
cell wall is not so small amount. In order to reveal the role of hemicellulose,
especially during cell wall biosynthesis, alteration of hemicellulose was tried.
First, UDP-glucuronic acid decarboxylase gene was inhibited by
RNAi-method. UDP-glucuronic acid decarboxylase (alias UDP-xylose synthase;
UXS) converts UDP-glucuronic acid to UDP-xylose, that is the source of xylose
constituent of xylan. Transgenic rice with RNAi-suppressed UXS showed
severely inhibited growth and no flower formation. Hemicellulose content
was reduced and cellulose content was slightly increased. Although gene
expression of only UXS was reduced by RNAi, the expression of lignin
biosynthesis related genes (PAL and 4CL) were also restricted. Lignin contents
of transgenic rice were also reduced according to the UXS expression level.
These results indicated that hemicellulose affects lignin biosynthesis.
The second point is two sugar transferase genes, glucuronic acid
xylan transferase (GUX) and xylan arabinosyl transferase (XAT). These two
enzymes transfer sugars to xylan backbone. Especially, arabinosyl side chain
binds with diferulic acids that bind to lignin through ester bond. Transgenic
rice with RNAi-suppressed and overexpressed in each GUX and XAT were
produced and analyzed. These transformants showed no growth inhibition
like in the case of UXS-RNAi transformants. The lignin biosynthesis related
genes were also affected by GUX- and XAT-RNAi plants, however, lignin
content was not impacted by these two genes.
49
Here the role of hemicellulose is discussed though transgenic rice

altered hemicellulose biosynthesis related genes.
Key words: rice, cell wall, hemicellulose, arabinoxylan, UDP-glucuronic acid

decarboxylase, transferase, glucuronic acid xylan transferase, xylan
arabinosyl transferase, lignin
50
Kenji Umemura
Research Institute for Sustainable Humanosphere

Kyoto University
Gokasho, Uji, Kyoto
Japan 611-0011
Office Tel : +81-774 38-3652
Office Fax : +81-774 38-3678
Email : umemura@rish.kyoto-u.ac.jp
FIELD OF RESEARCH
Wood adhesives
EDUCATION
1993 – 1996 Doctor of Agriculture Science, Division of Forest Engineering,
1991 – 1993 Master of Agricultural Science, Department of Agricultural

Chemistry,
Graduate School of Agriculture, Kinki University, Japan
1987 – 1991 Bachelor of Agriculture, Department of Chemistry,

Faculty of Agriculture, Kinki University, Japan
PUBLICATION
2019 Ragil Widyorini, Kenji Umemura, Dayu Kemalasari Soraya, Greitta

Kusuma Dewi, and Widyanto Dwi Nugroho. 2019 Effect of Citric
Acid Content and Extractives Treatment on the Manufacturing
Process and Properties of Citric Acid-bonded Salacca Frond
2018 Particleboard, BioResources, 14(2), 4171-4180
Zhongyuan Zhao, Yanfeng Miao, Ziqian Yang, Hua Wang, Ruijuan
Sang, Yanchun Fu,
51
Caoxing Huang, Zhihui Wu, Min Zhang, Kenji Umemura, Qiang

2018 Yong. 2018, Effects of
Sulfuric Acid on the Curing Behavior of Tannin-Sucrose Adhesive,
Polymer, 10(11), 1251, Doi: 10.3390/polym10060651
Miyuki Nakagawa, Akitaka Kimura, Kenji Umemura, Shuichi Kawai,
2017 Evaluation of
NO2 Sorption of Cedar Wood (Cryptomeria Japonica) with
Difference of the Specimen Size and Contact Condition between
NO2 Gas and Specimen by Using New Test System, J. Wood Sci.,
2017 64(3), 318-325 (2018)
Sukma S. Kusumah, Arinana Arinana, Yusuf S. Hadi, Ikhsan
Guswenrivo, Tsuyoshi Yoshimura, Kenji Umemura, Soichi Tanaka,
and Kozo Kanayama. 2017, Utilization of Sweet Sorghum Bagasse
and Citric Acid in the Manufacturing of Particleboard III: Influence
of Adding Sucrose on the Properties of Particleboard,
BioResources, 12(4), 7498-7514
Kenji Umemura, Shin Hayashi, Soichi Tanaka, Kozo Kanayama.
2017 Changes in physical and chemical properties of sucrose by
the addition of ammonium dihydrogen phosphate, J. Adhes. Soc.
Jpn., 53(4), 112-117
52
New Adhesion Technology for lignocellulose-based materials
Kenji Umemura1)
1)
Abstract
Global forest area continues to decrease in recent years, and 129

million hectares disappeared from 1990 to 2015. There is concern about the
future decrease by influence of climate change and excessive deforestation.
On the other hand, production of wood-based materials is expected to
increase in the future due to global population increase and economic
development. Therefore, utilization of unused lignocellulose such as
agricultural wastes as raw materials for wood-based materials should be
investigated.
As wood-based materials basically consist of wood elements and
adhesives, adhesives are essential for wood-based materials. The adhesive
properties greatly affect physical and mechanical properties of wood-based
materials manufactured. Currently, many types of synthetic resin adhesives
are being used in wood industry. Adhesives are generally synthesized from
various chemicals derived from fossil resources of naphtha and natural gas.
Considering global efforts to reduce the dependence on fossil resources, new
adhesion technology should be explored. The principal theories of wood
adhesion are mechanical, adsorption and chemical theories. In particular,
mechanical and adsorption theories are main theories when using synthetic
resin adhesives. This means that chemical reaction between an adhesive and
wood element hardly occur when actual manufacturing wood-based
materials.
Our laboratory found that citric acid which is derived from bio-
resources can be used as a wood adhesive. As results of instrumental
analyses, it was clarified that adhesion mechanism of citric acid was chemical
reaction between citric acid and wood constituents by esterification.
Therefore, it can be said that wood adhesion by using citric acid is new
technology.
53
In SATREPS project, particleboards using sweet sorghum bagasse

and citric acid have been researched. The effect of manufacture condition on
the mechanical and physical properties were clarified. The bending strength
and thickness swelling of the particleboard manufactured under optimum
condition were comparable to JIS A 5908 18-type standard. Nowadays, effort
toward social implementation for the particleboard is carried out.
Key words: wood-based materials, adhesive, citric acid, sweet shorghum

bagasse, particleboard
54
Yunimar
Indonesian Citrus and Subtropical Fruits Research Institute

Indonesian Agency for Agricultural Research and Development
Jalan Raya Tlekung no 1 Junrejo Batu East Java Indonesia
65327
Office tel : +62-341 592683
Office fax: +62-341 593047
Home tel : +62-823 3355 3087
Email: yunimar.lolit@gmail.com
FIELD OF RESEARCH
Microbiology
EDUCATION
1995 - 1999 : Bachelor, Department of Biology , FMIPA Andalas University
2006 - 2010 : Master, Entomology, IPB
2019 - …. : Student of Biology Doctoral Program, Brawijaya University
PUBLICATION
2015 Loop-mediated Isothermal Amplification (LAMP): Rapid
Detection of Huanglongbing in Citrus at Endemic Areas (Prosiding
Seminar Nasional Buah Tropika Nusantara II, bukittinggi)
2015 Potential of Trichoderma spp. As agents for controlling Fusarium
spp. On Strawberries ( Fragaria x ananassa Dutch ) (Jurnal
Hortikultura)
2017 Response of Aphis gossypii against some of Botanical Insecticides
Seminar Nasional dan Kongres Perhimpunan Hortikultura
Indonesia(Perhorti 2017)
2019 Diversity Of Endophytic Bacteria From Citrus Trifoliata
(Biodiversitas Journal of Biological Diversity)
55
Isolation And Characterization Of Endophyte Bacteria Producing

Enzyme Chitinase From Lemon Plants ( Citrus Limon )
(Biodiversitas Journal of Biological Diversity)
2019 Potency of Bacteria Endophytic Producing enzyme chitinase
Originally Citrus Lemon For Antifungal Botryodiplodia
theobromae (Proceedings of The Southeast Asia Plant Protection)
56
Development of Rapid Detection Kit for Seed Health Detection and

Endemicity Mapping of CVPD Disease
Yunimar1), Nurhadi1), B. D. Mariana1), O. Endarto1), Y. Sastro2), Hardiyanto2),

B.A. Fanshuri1)
1)
Indonesian Citrus and Subtropical Fruits Research Institute, Batu, East Java
2)
Indonesian Agency for Agricultural Research and Development, Bogor,
West Java
Abstract
Research entitled Development of Rapid Detection Kit to detect Seed Health
and Endemicity Mapping of CVPD Disease was carried out to address the
problem of CVPD caused by Candidiatus Liberibacter asiaticus (CLas), that is
severely threatening the Indonesian citrus industry particularly the small-
scale citrus growers who contribute approximately 80% of the country’s total
citrus production. The long-term goal is to develop a Rapid Detection Kit for
CVPD as an instrument for the establishment of community-based early
warning/response system towards CVPD outbreaks, and to develop operating
procedure for crisis/risk management towards CVPD outbreak as a model to
revitalize/rehabilitate citrus development areas currently endemic to CVPD.
Four research activities were conducted, namely: 1) evaluation and
validation of LAMP-based rapid detection kit of CVPD prototype; 2)
demonstration of application technique of rapid detection kit of CVPD; 3)
endemicity mapping of CVPD in citrus endemic area as a basis for generating
community-based early warning/response systems in managing CVPD
outbreaks; and 4) encouraging the role of citrus farmers and extension agents
towards the application of rapid detection kits of CVPD as the main
component in building point of care in managing CVPD towards the outbreaks
in endemic areas. Prototype of rapid detection kit resulted from this study
provides a specific, sensitive and rapid diagnostic tool for the distinction of
CVPD, with the potential to be standardized as a detection method for CLas
and will be very useful for monitoring the disease incidence in the field,
further suggesting the management strategies. The sensitivity of this LAMP
assay is very similar to the PCR method. This LAMP assay is simple, rapid, and
can be performed with a water bath or heating block. It is ideal for resource-
57
limited settings where CVPD is endemic. Compare to conventional PCR, the

LAMP assay reported here is easier to perform and more rapid, and the
results are easier to evaluate. The demonstration results concluded that 1)
CVPD rapid detection kits can be applied by participants who have no
expertise or experience in the molecular field with success rates above 95%;
2) rapid detection kit results of this evaluation met the ASSURED criteria so
that they can be applied in citrus development areas where detection
facilities are not available, and 3) rapid detection kits as a result of this
research can be developed as commercial products. At present, this CVPD
rapid detection kit has been registered to obtain property right for protection
from KEMENKUMHAM and has been patented with certificate no
IDP000053593 for a protection period of 20 years. On mapping study,
interactive maps that describe the geographical boundaries of Koto Tinggi
have been developed. Maps are processed through the Adobe Reader
application in the .pdf format so that by activating/ deactivating the virtual
layer, citrus farmers, field officers and other who interest, can see various
displays, including: geographical distribution of citrus plantations in the
development area, incidence of CVPD, prevalence of D. citri vector, seedling
status and other factors that influence the incidence of CVPD.
Keywords: citrus, CVPD, detection, disease, endemicity
58
Kuniyoshi Shimizu
Faculty of Agriculture Office Tel : +81-92 802-4673

Kyushu University Office Fax : +81-92 802-4673
Jl. Raya Bogor km 46 Cibinong, Home Tel : +81-90 9491-4444
Fukuoka Japan 819-0395 Email: shimizu@agr.kyushu-u.ac.jp
FIELD OF RESEARCH
Wood Chemistry, Bioactive Natural Product Chemistry
EDUCATION
1997– 2000 Doctor of Agriculture,
Graduate School of Agriculture, Kyushu University, Japan
1995 – 1997 Master of Agriculture,
Graduate School of Agriculture, Kyushu University, Japan
1991 – 1995 Bachelor of Agriculture,
Faculty of Agriculture, Kyushu University, Japan
PUBLICATION
2019 Hata, J., Ohara, T., Katakura, Y., Shimizu, K., Yamashita, S., Yoshida,
D., Honda, T., Hirakawa, Y., Shibata, M., Sakata, S., Kitazono, T.,
Kuhara, S., Ninomiya, T. Association Between Serum β-Alanine and
Risk of Dementia(2019) American journal of epidemiology, 188 (9),
2019 pp. 1637-1645.
Tan, H., Zhao, C., Zhu, Q., Katakura, Y., Tanaka, H., Ohnuki, K.,
Shimizu, K. Ursolic Acid Isolated from the Leaves of Loquat
(Eriobotrya japonica) Inhibited Osteoclast Differentiation through
2019 Targeting Exportin 5 (2019) Journal of Agricultural and Food
Chemistry, 67 (12), pp. 3333-3340.
El-Deen, A.K., Shimizu, K. Deep eutectic solvent as a novel disperser
in dispersive liquid-liquid microextraction based on solidification of
floating organic droplet (DLLME-SFOD) for preconcentration of
2019 steroids in water samples: Assessment of the method deleterious
59
impact on the environment using Analytical Eco-Scale and Green

Analytical Procedure Index(2019) Microchemical Journal, 149, art.
2019 no. 103988
Ashour, A., Amen, Y., Allam, A.E., Kudo, T., Nagata, M., Ohnuki, K.,
Shimizu, K. New isoindolinones from the fruiting bodies of the
fungus Hericium erinaceus, (2019) Phytochemistry Letters, 32, pp.
10-14.
Mwakalukwa, R., Ashour, A., Amen, Y., Niwa, Y., Tamrakar, S.,
Miyamoto, T., Shimizu, K.Anti-allergic activity of polyphenolic
compounds isolated from olive mill wastes (2019) Journal of
Functional Foods, 58, pp. 207-217.
60
Lifestyles of Health and Sustainability (LOHAS) concept for the better

future: How to utilize natural products as the valuable products-Medicine,
Functional Food, Aroma, Woody house-
Kuniyoshi Shimizu
Kyushu University, Motooka, Fukuoka 819-0395, Japan
Abstract
Our research group has been focusing on how to utilize natural

products based on LOHAS (Lifestyles of Health and Sustainability) concept
with relevant scientific evidence. Research fields include as follows. 1) Natural
Medicines, 2) Natural Cosmetics, 3) Natural functional foods, 4) Natural
aroma, 5) Natural dwelling space. Following topics will be discussed. 1. How
to utilize medicinal mushroom such as Ganoderma lingzhi for curing benign
prostate hyperplasia. 2. How to utilize the by-product in Olive oil
production. 3. How to utilize the aroma such as essential oil prepared from
the leaves of Abies sibiricaa or laurel (Laurus nobilis) leaves itself. 4. How to
utilize the wood as living materials, such as the wooden dwelling spaces using
naturally processed sugi (Cryptomeria japonica) wood boards
G. lingzhi has been the most well-studied species. G. lingzhi was
reported with a wide range of bioactivities including antioxidative, anti-aging,
immunomodulatory activity, anti-hepatotoxic, ant-angiogenic, anti-
histaminic, potential anticancer activity and even anti-HIV. Our group has
isolated many triterpenoids from reishi and reported their bioactivities,
including inhibition of benign prostatic hypertrophy, anti-androgenic
osteoclastogenesis, anti-diabetes, anti-diabetic complication,
antihypertension, inhibition of neuraminidase and anti-cancer activities. The
scientific evidence of their medicinal effect are discussed.
Recently, functional foods are increasingly gaining interest in the
food industry, as they provide the body with additional benefits as compared
to traditional foods. As a result, a large amount of by-products is generated
during their production process(es). Conventionally, these by-products have
been regarded as ‘wastes’. However, it is now known that they constitute
useful bioactive compounds which have the potential to be used as food
additives, cosmetics or in pharmaceuticals. During Olive oil production, one
61
of most commonly used ‘functional food’, several by-products are generated,

mainly olive wood and leaves, and olive mill wastes (OMW). How to utilize
OMW as functional product are discussed.
The pharmaceutical properties of aromatic herbs are partially
attributed to essential oils, which can be prepared from different parts of
aromatic plants by steam distillation, solvent extraction, mechanical
expression, and supercritical fluid and subcritical water extraction. The use of
essential oils has typically been limited to perfumes, cosmetics and food
flavourings. However, certain essential oils have been used to treat
psychiatric disorders such as anxiety, sleep disorders, and depression. How to
utilize the aromatic plant such as Abies sibirica, Chrysopogon zizanioides and
Laurus noblis with scientific evidences are discussed.
The woody house using the natural or low temperature dried solid
wood is relatively expensive, but it is a great advantage that volatile
components and moisture conditioning are maintained.The psychological
and physiological effect of the wooden dwelling spaces using Cryptomeria
japonica are discussed.
Key words: LOHAS; Natural Cosmetics; Natural Functional Foods; By-

producs; Natural Aroma; Natural dwelling space
62
Andria Agusta
Research Center for Biology Office Tel : +62-21-8765066

Indonesian Institute of Sciences (LIPI) Office Fax : +62-21-8765062
Jl. Raya Bogor km 46 Cibinong, Bogor Home Tel : +62-87770346919
Indonesia 16911 Email: andria.agusta@lipi.go.id
FIELD OF RESEARCH
Natural Product Chemistry
EDUCATION
2003 – 2006 Doctor of Pharmacy and Pharmaceutical Sciences, Graduate
School of Pharmacy and Pharmaceutical Sciences, Fukuyama
University, Japan
2001 – 2003 Master of Pharmacy and Pharmaceutical Sciences, Graduate
School of Pharmacy and Pharmaceutical Sciences, Fukuyama
University, Japan
1988 – 1993 Bachelor in Chemistry, Faculty of Mathematic and Natural
Sciences, Andalas University, Indonesia
PUBLICATION
2019 M Ilyas, D Wulansari, A Fathoni, A Agusta. 2019. An assemblages

of fungal endophytes isolated from medicinal plants collected
from Toba and Samosir, North Sumatra. IOP Conference Series:
Earth and Environmental Science 308 (1), 012070.
Praptiwi, AL Putri, D Wulansari, A Agusta. 2019. Assessment of

actinomycetes isolated from soils on Simeuleu Island as
antibacterial and antioxidant. American Institute of Physics
Conference Series 2120 (8).
R Budiarto, R Poerwanto, E Santosa, D Efendi, A Agusta. 2019.

Agronomical and Physiological Characters of Kaffir Lime (Citrus
63
hystrix DC.) Seedling under Artificial Shading and Pruning.

Emirates Journal of Food and Agriculture, Vol. 31, no. 3, May 2019,
pp. 222-30.
Fathoni A. and Agusta A. 2019. Bioproduction of cytochalasin

D by endophytic fungus Xylaria sp. DAPKRI-5. J. Appl. Pharm
Sci. 9 (03), 105-110.
Maehara, S., Agusta, A., Tokunaga, Y. Shibuya H. Hata T. 2019.

Endophyte composition and Cinchona alkaloids production
abilities of Cinchona ledgeriana cultivated in Japan. J. Nat.
Med., 73: 431. https://doi.org/10.1007/s11418-018-1273-z
Kamitakahara H, Okayama O, Praptiwi, Agusta A, Tobimatsu Y,

Takano T. 2019. Twi dimensional NMR analysis of Angiopteris
evecta rhizome and improved extraction for angiopteroside.
Phytochmeical Analysis, 30(1), 95-100.
https://doi.org/10.1002/pca.2794
2018 Sumiasih IH, Arzam TS, Poerwanto R, Efendi D, Agusta A., Yuliani
S. 2018. Study of pigment accumulation beta-crypatoxanthin for
orange color formation on tropical citrus fruits. Indonesian Journal
of Horticulture, 9(2), 73-83.
http://dx.doi.org/10.29244/jhi.9.2.%25p
Praptiwi, Raunsai M, Wulansari D, Fathoni A, and Agusta A. 2018.

Antibacterial and antioxidant activities of endophytic fungi
extracts of medicinal plants from Central Sulawesi. Journal of
Applied Pharmaceutical Science Vol. 8(08), 069-074.
Sumiasih IH., Poerwanto R., Efendi D., Agusta A., and Yuliani S.
2017 2017. The Analysis of β-cryptoxanthin and Zeaxanthin using HPLC
in the Accumulation of Orange Color on Lowland Citrus.
International Journal of Applied Biology, 1(2), 37-45.
64
Musdja MY., Hapsari MA. and Agusta A. 2017. Comparison of

Activity and Inhibitory Mechanism between (+)-Catechin and
Water Extract of Gambier (Uncaria Gambir Roxb.) Against Some
Bacteria. Scientific Journal of PPI-UKM, 4(2), 55-60. DOI:
10.27512/sjppi-ukm/se/a29012018.
Anita S., Agusta A., Farida WR., Nugroho N., and Wulansari D.
2017. A Preliminary Study of Aphrodisiac Property from Porcupine
Tail Meat Ethanol Extract in Male Mice. Zoo Indonesia 26 (1), 52-
58.
2016
Maehara S, Agusta A, Kitamura C, Ohashi K, Shibuya H. 2016.
Composition of the endophytic filamoentous fungi associated
with Cinchona ledgeriana seeds and production of cinchona
alkaloids. Journal of Natural Medicines. 70, 271-275.
Dewi Wulansari, Praptiwi, Heddy Julistiono, Arif Nurkanto and

Andria Agusta. 2016. Antifungal activity of (+)-2,2’-epicytoskyrin A
and its effect on fungal cells. Makara Journal of Science, 20(4),
160-166.
Andria Agusta, Dewi Wulansari, Praptiwi, Arif Nurkanto, dan

Ahmad Fathoni. 2015. Antibacterial Activity and Mode of Action
of (+)-2,2’-Epicytoskyrin A. Microbiology Indonesia, 9:1, 35-43.
Praptiwi, Muhammad Ilyas, Ahmad Fathoni, Dewi Wulansari dan

Andria Agusta. 2015. Antibacterial Screening of the Culture of
Endophytic Fungi Extracts Isolated from Cinnamon Stick
(Cinnamomum burmannii [Nees & T. Nees] Blume). Teknologi
Indonesia, 38 (1) : 33–41.
Nurkanto A. and A. Agusta. 2015. Molecular Identification and

Morpho-Physiological Characterization of Actinomycetes with
Antimicrobial Properties. Jurnal Biologi Indonesia, 11(2), 195-203.
65
Bioprospecting from Some Rural Areas and Small Islands of Indonesia
Andria Agusta
Research Center for Biology, LIPI, Cibinong, Bogor, Indonesia
Abstract
Exploration of bioresources in several rural areas and small islands

in the Indonesian region was carried out in three consecutive years of 2016-
2018. During exploration, we collected data related to traditional knowledge
about medicinal plants used by local people for generations. We also
collected several types of medicinal plants found at that location, and some
species included isolation of endophytic fungi which were associated with
these medicinal plants. Also we isolate microbes from soil samples. In the
laboratory, all plant and microbial samples collected were screened for their
biological activity as antibacterial and antioxidant by applying the rapid direct
TLC-bioautography technique. Some interesting samples for their biological
activities as antibacterial or antioxidant are followed by a more detailed and
in-depth study to uncover their bioprospection.
Key words: bioresources, medicinal plant, endophytic microbes, soil

microbes, antibacterial, antioxidant.
66
Dede Heri Yuli Yanto

Senior Researcher
Research Center for Biomaterials

Indonesian Institute of Sciences (LIPI)
Cibinong Science Center, Cibinong, Bogor
Indonesia 16911
Office Tel : +62-21-87914511
Office Fax : +62-21-87914510
Email: dede@biomaterial.lipi.go.id
FIELD OF RESEARCH
Bioprocess Technology and Bioremediation
EDUCATION
2011 – 2014 Doctor of Philosophy, The United Graduate School of
Agricultural Sciences, Ehime University, Japan
2009 – 2011 Master of Agriculture, Department of Applied Bioscience,
Faculty of Agriculture, Ehime University, Japan
1999 – 2004 Bachelor of Science, Department of Chemistry,
Faculty of Mathematics and Natural Sciences, Bogor
Agricultural University, Indonesia
PUBLICATION
2019 Fenny Clara Ardiati, Dede Heri Yuli Yanto, Sita Heris Anita, Takashi
Watanabe. Immobilization of Trametes hirsuta D7 in light expanded
clay aggregate for decolorization of synthetic dye. IOP Conf. Series:
Earth and Environmental Science 308: 012002.
2019 Dede Heri Yuli Yanto, Ni Putu Ratna Ayu Krishanti, Fenny Clara
Ardiati, Sita Heris Anita, Indra Kusuma Nugraha, Fahriya Puspita Sari,
Raden Permana Budi Laksana, Sulitiyani Sapardi, Takashi Watanabe.
Biodegradation of Styrofoam waste by ligninolytic fungi and
bacteria. IOP Conf. Series: Earth and Environmental Science 308:
012001.
67
2019 Sita Heris Anita, Fahriya Puspita Sari, Dede Heri Yuli Yanto.
Decolorization of synthetic dyes by ligninolytic enzymes from
Trametes hirsuta D7. Makara Journal of Science 23 (1): 44–50.
2019 Fahriya Puspita Sari, Dede Heri Yuli Yanto, Gustan Pari. Activated
carbon derived from OPEFB by one step steam activation and its
application for dye adsorption: Kinetics and Isothermal Studies.
Reaktor 19 (2): 67–75.
2018 Asep Hidayat, Dede Heri Yuli Yanto. Biodegradation and metabolic
pathway of phenanthrene by a new tropical fungus, Trametes
hirsuta D7. Journal of Environmental Chemical Engineering, 6
(2): 2454–2460
2017 Dede Heri Yuli Yanto, Asep Hidayat, Sanro Tachibana. Periodical
biostimulation with nutrient addition and bioaugmentation using
mixed fungal cultures to maintain enzymatic oxidation during
extended bioremediation of oily soil microcosms. International
Biodeterioration & Biodegradation, 116: 112–123.
2017 Dede Heri Yuli Yanto, Syifa Zahara, Raden Permana Budi
Laksana, Sita Heris Anita, Maulida Oktaviani, Fahriya Puspita Sari.
Development of PVA-alginate as a matrix for enzymatic
decolorization of textile dye in bioreactor system. AIP Conference
Proceedings, 1803: 020062.
2016 Dede Heri Yuli Yanto, Sanro Tachibana. Utilization of kapok fiber as
a natural sorbent in petroleum hydrocarbon biodegradation by
Pestalotiopsis sp. Journal of Lignocellulose Technology, 1. 68–73.
2014 Dede Heri Yuli Yanto, Sanro Tachibana. Potential of fungal co-
culturing for accelerated biodegradation of petroleum hydrocarbons
in soil. Journal of Hazardous Materials, 278: 454 – 463.
2014 Dede Heri Yuli Yanto, Sanro Tachibana. Enhanced biodegradation of
asphalt in the presence of Tween surfactants, Mn 2+ and H2O2 by
Pestalotiopsis sp. in liquid medium and soil. Chemosphere, 103:
105–113.
2014 Dede Heri Yuli Yanto, Sanro Tachibana, Kazutaka Itoh.
Biodecolorization of textile dyes by immobilized enzymes in a
vertical bioreactor system. Procedia Environmental Sciences, 20:
235–244.
68
2013 Dede Heri Yuli Yanto, Sanro Tachibana. Biodegradation of petroleum

hydrocarbons by a newly isolated Pestalotiopsis sp. NG007.
International Biodeterioration & Biodegradation, 85: 438–450.
2013 Dede Heri Yuli Yanto, Sanro Tachibana, Kazutaka Itoh.
Biodecolorization and biodegradation of textile dyes by the newly
isolated saline-pH tolerant fungus Pestalotiopsis sp. Journal of
Environmental Science and Technology, 7 (1): 44–55.
69
The Potential for tropical white-rot fungi in the treatment of crude oils and
textile dyes
Dede Heri Yuli Yanto
Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI),

Jl. Raya Bogor Km. 46, Cibinong Science Center, Cibinong, Bogor 16911,
Indonesia
*Corresponding author: dede@biomaterial.lipi.go.id
Abstract.
Environmental pollution by crude oils and textile dyes has become serious
problem due to the ecological damage they cause. Biodegradation is the
dominant process used to reduce the effect of crude oils and textile dyes on
the environment. Due to the complex chemical structure of crude oils and
textile, identifying microorganism capable of degrading these pollutants is
important. White-rot fungi (WRF) are unique organisms that show the ability
to degrade/mineralize toxic environmental pollutants such as crude oils and
dyes. The key enzymes of their metabolism are extracellular ligninolytic
enzymes. Laccase is one important extracellular enzyme produced by WRF
due to a broad substrates specificity and ability to oxidize several xenobiotic
compounds. In this study, degradation of crude oils and textile dyes by
tropical WRF were discussed.
Keywords: Biodegradation, crude oils, decolorization, textile dyes, white-rot

fungi, laccase
70
Aya YANAGAWA
Research Instituter for Sustainable Humashpere

Kyoto University Gokashou, Uji
Japan 611-0011
Office Tel : +81-774-38-3664
Office Fax : +81-774 38-3666
Email : ayanagawa@rish.kyoto-u.ac.jp
FIELD OF RESEARCH
Microbial Control and Insect Pathology
EDUCATION
2006 – 2009 Doctor of Agricultural Science, Department of Applied
Genetics & Pest Management,
Kyushu University, Japan.
2004 – 2006 Master of Agricultural Science, Department of Applied
Kyushu University, Japan.
2000 – 2004 Bachelor in Agricultural Science, Department of Applied
Faculty of Agriculture, Kyushu University, Japan
PUBLICATION
2019 Hanasaki, N., Iwasa, I., Takahashi, Y., Yanagawa, A., Kajiwara, S.,
Takikawa, Y., Matsukawa, T., Arai, S., Akino, T. Antimicrobial
activities by floral scents of the Japanese bamboo lily Lilium
japonicum, the symbolic flowers offered the deity at the Saikusa
2019 Festival. AROMA RESEARCH 78 (20), 41-47. (in Japanese with
English summery)
Mizuki Fujisawa, Naohito Hanasaki, Yoshihiro Takikawa, Aya
Yanagawa, Tetsuya Matsukawa, Toshiharu Akino, Shin'ichiro
71
Kajiyama and Shigeru Arai. 2019. A Questionnaire-based

2019 Investigation Indicated Mental Relaxation Effects in the Scent of
the Sacred Flower of the Saikusa Festival, Lilium japonicum,
Japanese Journal of Pharmacology 73(2). 1-6. (in Japanese with
2018 English summery)
Yanagawa, A., Couto, A., Sandoz, JC., Hata, T., Mitra, A., Agha,
M.A., Marion-Poll, F., LPS perception through taste-induced
2018 reflex in Drosophila melanogaster, Journal of Insect Physiology
112. 39-47.
Karim MR, Yanagawa A, Ohinata K. 2018. Soy undecapeptide
2017 induces Drosophila hind leg grooming via dopamine receptor,
Biochemical and Biophysical Research Communications 499. 454-
458.
2016 Yanagawa A, Chabaud M, Imai T, Marion-Poll F. 2018. Olfactory
cues play a significant role in removing fungus from the body
surface of Drosophila melanogaster, Journal of Invertebrate
Pathology151. 144-150.
2016 Yanagawa A, Neyen C, Lemaitre B, Marion-Poll F. 2017. The
gram- negative sensing receptor PGRP-LC contributes to
2015 grooming induction in Drosophila, PlosOne 12(11). e0185370.
doi: 10.1371/journal.pone.0185370.
2015 Fukuda K, Yanagaw A, Tuda M, Sakurai G, Kamitani S, Furuya N.
2016. Sexual difference in antennal sensilla abundance, density
and size in Callosobruchus rhodesianus (Coleoptera:
2014 Chrysomelidae: Bruchinae), Applied Entomology and Zoology 51.
641-651. doi.10.1007/ s13355-016-0441-4.
Yanagawa A. 2016. Seawater influence monitored by NaCl on
2014 the growth of Trametes versicolor, Environmental Science and
Pollution Research. 23(1). 932-937.
French A, Agha MA, Mitra A, Yanagawa A, Sellier M-J, Marion-
2014 Poll F. 2015. Drosophila bitter taste(s), Front. Integr. Neurosci. 9,
article 58, doi: 10.3389/fnint.2015.00058.
72
Yanagawa A, Imai T, Akino T, Toh Y, Yoshimura T, Olfactory cues

from pathogenic fungus affect the choice of moving direction of
2013 termites, Coptotermes formosanus, Journal of Chemical Ecology,
41(12), 1118-1126.
2012 Yanagawa A, Guigue A, Marion-Poll F. 2014. Hygienic grooming
is induced by contact chemicals in Drosophila melanogaster,
Front. Behv. Neurosci. 8. article 254. doi: 10.3389/
2012 fnbeh.2014.00254
Yanagawa A, Yoshimura T. 2014. 6.1.6.3. Insect control by
2012 microwave. Chapter6. Application and utilization of microwave,
Sangyou gijutu serves center, Tokyo, Japan (in Japanese), 666-
2011 670.
Yanagawa A, Kashimura K, Mitani T, Shinohara N, Yoshimura T.
2014. Influence of Powerful Microwaves on the Termite
2011 Coptotermes formosanus -Impact of Powerful Microwaves on
Insects in Processing and properties of advanced ceramics and
composites VI / edited by J.P. Singh (Ceramic transactions , v.
2010 249). 367-374.
Zhukovskaya M, Yanagawa A, Forschler BT. 2013. review:
2010 Grooming behavior as a mechanism of insect disease defense.
Insects 4. 609-630. doi:10.3390/insects4040609
Yanagawa A, Fujiwara-Tsujii N, Akino T, Yoshimura T, Yanagawa
2010 T, Shimizu S. 2012. Odor aversion and pathogen-removal
efficiency in grooming behavior of the termite Coptotermes
2009 formosanus, PlosOne 7(10): e47412.
Yanagawa A. 2012. Entomopathogenic fungi as a termite control
agents, TRG 9: Pacific Rim Termite Research Group, Hanoi,
2009 Vietnam.
Yanagawa A. 2012. 2.3. Resistance mechanism to fungal
infection in termites. In Chapter 2. Termite Dictionary, Kaiseisha
2009 Press., Hyougo, Japan (in Japanese), 61-68.
73
Yanagawa A, Fujiwara-Tsujii N, Akino T, Yoshimura T, Yanagawa

2009 T, Shimizu S. 2011. Musty odor of entomopathogens enhances
disease-prevention behaviors in the termite Coptotermes
formosanus, Journal of Invertebrate Pathology 108: 1-6.
2008 Yanagawa A, Fujiwara-Tsujii N, Akino T, Yoshimura T, Yanagawa
T, Shimizu S. 2011. Behavioral changes in the termite,
Coptotermes formosanus (Isoptera), inoculated with six fungal
2008 isolates, Journal of Invertebrate Pathology107: 100-106.
Yanagawa A, Yokohari F, Shimizu S. 2010. Influence of fungal
2007 odor on grooming behavior of the termite, Coptotermes
formosanus Shiraki. Journal of Insect Science (10), article4.
2007 Yanagawa A, Yokohari F, Tsunoda K, Imamura Y, Yoshimura T.
2010. Role of antennae to detect humidity in the termite,
Coptotermes formosanus, TRG 7, 2010: Pacific Rim Termite
2007 Research Group, Quality Hotel Singapore, Singapore.
Yanagawa A, Yoshimura T, Yanagawa T, Yokohari F. 2010.
2005 Detection of humidity difference by antennae in the termite,
Coptotermes formosanus. Sociobiology 56 (1). 255-270.
Yanagawa A, Iiyama K, Yasunaga-Aoki C, Shimizu S. 2009. Review
article: Defense Mechanism of The Termite, Coptotermes
formosanus Shiraki to Entomopathogenic Fungi. Entomotec 35.
9-15. (Written in Japanese).
Yanagawa A, Shimizu S, Noma K, Nishikawa M, Ogino K, Yokohari
F. 2009. Classification and distribution of antennal sensilla of the
termite, Coptotermes formosanus Shiraki. Sociobiology 54(2).
327-349.
Yanagawa A, Yokohari F, Iiyama K, Yasunaga-Aoki C, Shimizu S.
2009. Antennal hygroreception of the termite, Coptotermes
formosanus, TRG 6, 2009: Pacific Rim Termite Research Group,
Kyoto, Japan.
74
Yanagawa A, Yokohari F, Shimizu S. 2009. The role of antennae

in removing entomopathogenic fungi from cuticle of the termite,
Coptotermes formosanus. Journal of Insect Science (9). article 6.
Yanagawa A, Iiyama K, Yasunaga-Aoki C, Shimizu S. 2008. Innate
humoral immunity of the termite, Coptotermes formosanus
Shiraki to Metarhizium anisopliae. Entomotec 32. 51-54. (Written
in Japanese with English summary)
Yanagawa A, Yokohari F, Shimizu S. 2008. Defense mechanism of
the termite, Coptotermes formosanus Shiraki, to
entomopathogenic fungi. J. Inver. Pathol. 97. 165-170.
Yanagawa A, Shimizu S. 2007. Detection of entomopathogenic
fungus conidia in alimentary tracts of the termite, Coptotermes
formosanus. Jpn. J. Environ. Entomol. Zool. 18. 161-168.
Yanagawa A, Iiyama K, Yasunaga-Aoki C, Shimizu S. 2007.
Defense strategy of the termite, Coptotermes formosanus Shiraki
to Metarhizium anisopliae. Entomotec 31. 29-32. (Written in
Japanese).
Yanagawa A, Shimizu S. 2007. Resistance of the termite,
Coptotermes formosanus Shiraki to Metarhizium anisopliae due
to grooming. BioControl 52 (1). 75-85.
Yanagawa A, Shimizu S. 2005. Defense strategy of the termite,
Coptotermes formosanus Shiraki to entomopathogenic fungi.
Jpn. J. Environ. Entomol. Zool. 16: 17-22.
75
Energy transit petterns of 2.45GHz microwaves in termite body,

Coptotermes formosanus
Aya Yanagawa1)
1)
Kyoto University, Uji, Kyoto 611-0011, Japan (text in 10.5 p)
Abstract (12p, bold)
Heat heating is a chemical-free method for killing pest insects,

whose demands are increasing. Heat treatment, however, always face the
issue of labor-intensive and energy-consuming. To reduce the energy
consumption, it is essential to enhance its efficiency to kill insects. Heat
treatment with microwaves is better to produce heat energy in a short time
and thus tend to apply the low-water content products. Today, the parameter
used in microwave treatment is generally the temperature of irradiated
materials. Clarifying the physical influence of microwave on target pests
might bring a new possibility of better irradiation method. In this study, we
examined how irradiation conditions influence the conversion of
electromagnetic energy to heat when 2.45 GHz microwaves penetrate the
body of the termite, C. formosanus. The results indicated that it is possible to
change the energy transition efficiency by irradiation conditions. Termite
seems to be tolerant to direct irradiation of microwaves and thus their
physical features were investigated.
Key words (4-6 keywords 10.5p bold): non-chemical treatment, 2.45GHz

microwave, resonance waves, travelling waves, C. formosanus
76
Mamoru Kanzaki
Division of Forest and Biomaterials Science Office Tel : +81-75-753-6071

Graduate School of Agriculture Email: mkanzaki@gmail.com
Kyoto University
Kyoto 606-8502, Japan
FIELD OF RESEARCH
Forest Ecology and Management
EDUCATION
1982 – 1985 Doctor of Science, Graduate School of Science
Osaka City University, Japan
1982 – 1980 Master of Science, Graduate School of Science,
Chiba University, Japan
1975 – 1979 Bachelor of Agriculture, Faculty of Agriculture,
Tokyo University of Agriculture and Technology, Japan
PUBLICATION
2017
Mya Thandar Toe, Mamoru Kanzaki, Tsung-Hsun Lien, and
Ke-Sheng Cheng. 2017. Spatial and Temporal Rainfall
Patterns in Central Dry Zone, Myanmar: A Hydrological Cross-
Scale Analysis. Terrestrial, Atmospheric and Oceanic Sciences
2017
Journal. 28(3): 425-436. DOI: 10.3319/TAO.2016.02.15.01(Hy)
Phyu Phyu Lwin, Mamoru Kanzaki 2017. Species composition,
diversity, and stand structure of tropical lower montane
2017
forests resulting from various human impacts on the Shan
Plateau, eastern Myanmar, Tropics 26:71-82.
doi.org/10.3759/tropics.MS17-03
2018
77
Tomoya Inada, Kaoru Kitajima, Suryo Hardiwinoto and

Mamoru Kanzaki 2017. The Effect of Logging and Strip
Cutting on Forest Floor Light Condition and Following Change.
2019 Forests 2017, 8, 425; doi:10.3390/f8110425
Sarasa Amma, Hirokazu Toju, Chongrak Wachrinrat, Hirotoshi
Sato, Akifumi S. Tanabe, Taksin Artchawakom, and Mamoru
Kanzaki 2018. Composition and Diversity of Soil Fungi in
Dipterocarpaceae-Dominated Seasonal Tropical Forests in
Thailand. Microbes Environ. Vol. 33, No. 2, 135-143.
doi:10.1264/jsme2.ME17168
Md Mizanur Rahman, David Lagomasino, SeungKuk Lee,
Temilola Fatoyinbo, Imran Ahmed & Mamoru Kanzaki 2019.
Improved assessment of mangrove forests in Sundarbans East
Wildlife Sanctuary using WorldView 2 and TanDEM-X high
resolution imagery. Remote Sensing in Ecology and
Conservation, doi: 10.1002/rse2.105.
78
Introduction of JASTIP Project
Mamoru Kanzaki
Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan
Abstract
JASTIP (Japan-ASEAN Science, Technology and Innovation

Platform) is a Kyoto University Project funded by Japan Science and
Technology Agency. The project aims to promote the Japan-ASEAN
collaboration in Science and Technology, especially in the field of Energy and
Environment Science, Bioresouces and Biodiversity Studies, and Disaster
Prevention Studies. JASTIP establish joint laboratories in collaboration with
Indonesian Institute of Sciences (LIPI, Indonesia), National Science and
Technology Development Agency (NSTDA, Thailand) and Malaysia-Japan
International Institute of Technology (MJIIT, Malaysia). The Joint laboratory
in LIPI established in the Research Center for Biomaterials and the Research
Center for Biology focuses on the bioresources and biodiversity.
Bioresouces and Biodiversity Studies in JASTIP have been carried
out in collaboration with LIPI, NSTDA, Chulalongkorn University, Kasetsart
University, and other ASEAN and Japanese universities and institutions. We
are conducting the biodiversity surveys and monitoring, ecological and
taxonomical studies for biodiversity conservation, and the bioprospecting of
functional chemicals in plants and microbes. We are also developing the
technology for processing wood materials, bioprocessing of the industrial
waste, and processing cellulose and lignin for various products. All of our
works is to conserve the biodiversity and to establish the sustainable use of
bioresources and genetic resources. The effective utilization of bioresoueces,
including genetic resources is a key for the sustainable development of
ASEAN countries and also the sustainable future of the world. It also
contributes to the achievement of low carbon society.
Besides of these research activities, JASIP’s another mission is to
establish the platform to enhance the collaboration between ASEAN
researchers, between ASEAN and Japanese researchers and between
academia, industry, and government sectors. We repeated workshops,
symposiums and seminars in Japan and ASEAN countries and also financially
support the activities aim to expand and strengthen these collaborations
through JASTIP-NET.
79
In this session, three speakers present the achievement from JASTIP

and JASTIP-NET activities. These presentations will illustrate the scheme of
JASTIP well.
Key words: science, technology, innovation, sustainable development,

ASEAN, Japan, biodiversity, bioresource
80
Takashi Watanabe
Research Institute for Office Tel : +81-774-38-3640

Sustainable Humanosphere (RISH) Office Fax : +81-774-38-3681
Kyoto University Home Tel : +81-90-6966-8429
Gokasho, Uji, Kyoto 611-0011, Japan Email: twatanab@rish.kyoto-u.ac.jp
FIELD OF RESEARCH
Biomass Conversion
EDUCATION
1989 Doctor of Agric., Kyoto Univ.
1982 – 1984 Graduate School of Agric., Kyoto Univ.
1978 – 1982 Bachelor in Faculty of Agric., Kyoto Univ.
PUBLICATION
2019 Tokunaga, Y., T. Nagata, T. Suetomi, S. Oshiro, K. Kondo, M. Katahira

and T. Watanabe, NMR analysis on molecular interaction of lignin
with amino acid residues of carbohydrate-binding module from
2018 Trichoderma reesei Cel7A, Scientific Reports, 9, 1977 (2019).
Nishimura, H., A. Kamiya, T. Nagata, M. Katahira and T. Watanabe,
Direct evidence for alpha ether linkage between lignin and
carbohydrates in wood cell walls, Scientific Reports, 8, 6538 (2018).
Yoshioka, K., M. Daidai, Y. Matsumoto, R. Mizuno, Y. Katsura, T.
Hakogi, H. Yanase and T. Watanabe, Self-sufficient bioethanol
production system using a lignin-derived adsorbent of fermentation
inhibitors, ACS Sustain. Chem. Eng., 6, 3070–3078 (2018).
Li, R., R. Narita, H. Nishimura, S. Marumoto, S. P. Yamamoto, R.
2017 Ouda, M. Yatagai, T. Fujita,T. Watanabe, Antiviral activity of
phenolic derivatives in pyroligneous acid from hardwood, softwood,
and bamboo, ACS Sustain. Chem. & Eng., 6, 119–126 (2018).
Qu, C., M. Kaneko, K. Kashimura, K. Tanaka, S. Ozawa, T. Watanabe,
Direct production of vanillin from wood particles by copper oxide–
81
peroxide reaction promoted by electric and magnetic fields of

2016 microwaves. ACS Sustain. Chem. Eng., 5, 11551–11557 (2017).
Kaiho, A., D. Mazzarella, M. Satake, M. Kogo, M., R. Sakai, T.
Watanabe, Construction of di(trimethylolpropane) cross linkage and
2015 phenylnaphthalene structure coupled with selective β-O-4 bond
cleavage for synthesizing lignin-based epoxy resins with controlled
glass transition temperature. Green Chemistry, 18, 6526-6535
2014 (2016).
Yamaguchi, A., K. Isozaki, M. Nakamura, H. Takaya, T. Watanabe,
Discovery of 12-mer peptides that bind to wood lignin. Scientific
Reports, 6, 21833 (2016).
Kaiho A., M. Kogo, R. Sakai, K. Saito and T. Watanabe, In situ
trapping of enol intermediates with alcohol during acid-catalysed
de-polymerisation of lignin in a nonpolar solvent, Green Chemistry,
17, 780-2783 (2015).
Nishiwaki-Akine, Y., T. Watanabe: Dissolution of wood in α-keto acid
and aldehydic carboxylic acids and fractionation at room
temperature, Green Chemistry, 16, 3569-3579 (2014).
82
e-Asia Project “Integrated Biorefinery of Sugarcane Trash”
Takashi Watanabe
Abstract
Sugarcane trash is an agricultural residue underused in Southeast Asia

(SEA) and other tropical and subtropical regions. This cooperative project
aims to study an integrated biorefinery model for efficient utilization of
sugarcane trash for conversion of the residue to biofuels and chemicals by
combination of bio- and chemical processing. Social LCA is studied to show
the impact of the proposed model on regional society and economy. The
83
biomass is initially fractionated to its constituents by microwave-accelerated

acid catalysis, in comparioson with other conventional pretreatmens.
Research is conducted to optimize the pretreatment process with the key
focus on yields, selectivity and properties of the separated fractions for
subsequent processing. Structures of the separated fractions are analyzed by
2D-NMR, mass spectrometry and other advanced analytical methods. The
respective lignocellulose-derived fractions are converted to isobutanol,
xylitol, steroselective lactic acid and biosurfactant through the development
of enzymes, cell factories, biocatalysts, and chemical catalytic reactions.
Biochemical characterization of auxiliary enzymes (i.e. AA9 lytic
polysaccharide monooxygenase) and design of synergistic enzyme system
using hydrolases and the oxygenase on saccharification is conducted.
Metabolic flux and design of iso-butanol production flow is studied in
collaboration of NSTDA and Kyoto University. Non-productive adsorption of
carbohydrate-degrading enzymes to lignin is one of the major obstacles for
industrialization of biorefinery process through enzymatic saccharification
and fermentation. Novel surfactants composed of the lignin with hydrophilic
polymers such as polyvinyl alcohol are synthesized and their suppressive
effects on the enzymatic hydrolysis are evaluated. The integrated process is
evaluated and compared to conventional use of sugarcane trash in
cogeneration of heat/electricity by using the social-LCA method. Data on
sugarcane plantation, processing, and waste generation is collected in
Thailand, Indonesia and Laos, and assesed the socioeconomic impact of the
integrated process under local scenarios.
Key words: Sugarcane bagasse, biorefinery, biomass, social LCA analysis
84
Euis Hermiati
Research Center for Biomaterials Office Tel : +62-21 8791-4511

Indonesian Institute of Sciences (LIPI) Office Fax : +62-21 8791-4510
Jl. Raya Bogor Km 46, Cibinong, Mobile Tel : +62-8128644216
Bogor 16911 Indonesia Email: euis.hermiati@lipi.go.id
FIELD OF RESEARCH
Biomass process technology
EDUCATION
2008 – 2012 Doctor of Agroindustrial Technology,
Graduate School, Bogor Agricultural University, Indonesia
1986 – 1989 Master of Science (Food Science), Graduate School,
Rutgers University, USA
1979 – 1983 Sarjana of Technology of Agricultural Products, Faculty of
Agricultural Technology, Bogor Agricultural University,
Indonesia
PUBLICATIONS
2019
Anita SH, Fitria, Solihat NN, Sari FP, Risanto L, Fatriasari W, Hermiati
E. 2019. Optimizatin of microwave-assisted oxalic acid pretreatment
of oil palm empty fruit bunch for production of fermentable sugars.
Waste and Biomass Valorization. https://doi.org/10.1007/s12649-
018-00566-w
Oktaviani M, Hermiati E, Thontowi A , Laksana RPB, Kholida LN,
Andriani A, Yopi, Mangunwardoyo W. 2019. Production of xylose,
glucose, and other products from tropical lignocellulose biomass by
using maleic acid pretreatment. Proceedings The 2nd International
2018 Conference on Natural Products and Bioresource Science 2018. IOP
Conference Series: Earth and Environmental Science 251(1): 012013.
85
Fatriasari W, Raniya R, Oktaviani M, Hermiati E. 2018. The

improvement of sugar and bioethanol production of oil palm empty
fruit bunches (Elaeis guineensis Jacq) through microwave-assisted
maleic acid pretreatment. BioResources 13: 4378-4403.
Rahmani N, Kahar P, Lisdiyanti P, Hermiati E, Lee J, Yopi, Prasetya B,
Ogino C, Kondo A. 2018. Xylanase and feruloyl esterase from
actinomycetes cultures could enhance sugarcane bagasse hydrolysis
in the production of fermentable sugars. Bioscience, Biotechnology,
Biochemistry 82(5): 904-915.
Hermiati E, Solihat NN, Adi DTN, Ghozali M. 2018. The Ultrastructure
and enzyme susceptibility of oil palm empty fruit bunch pretreated by
oxalic acid under microwave irradiation. Proceedings 5th
International Symposium on Innovative Bio-Production Indonesia,
Bogor, October 10th, 2018, pp. 92-99.
Fatriasari W, Adi DTN, Laksana RPB, Fajriutami T, Raniya R, Ghozali M,
Hermiati E. 2018. The effect of amphipilic lignin derivatives addition
2017
on enzymatic hydrolysis performance of kraft pulp from sorghum
bagasse. IOP Conf. Series: Earth and Environmental Science 141 (2018)
012005
Risanto L, Fitria, Fajriutami T, Hermiati E. 2018. Enzymatic
saccharification of liquid hot water and dilute sulfuric acid pretreated
oil palm empty fruit bunch and sugarcane bagasse. IOP Conf. Series:
Earth and Environmental Science 141 (2018) 012025
Solihat NN, Sari FP, Risanto L, Anita SH, Fitria, Fatriasari W, Hermiati
E. 2017. Disruption of oil palm empty fruit bunches by microwave-
assisted oxalic acid pretreatment methods. J. Math. Fund. Sci. 49, 1-
14. DOI: 10.5614/j.math.fund.sci.2017.49.X.X
Pramasari DA, Haditjaroko L, Sunarti TC, Hermiati E, Syamsu K. 2017.
The effectiveness of physical and alkali hydrothermal pretreatment in
improving enzyme susceptibility of sweet sorghum bagasse. Jurnal
2014 Bahan Alam Terbarukan 6(2): 117-131.
Solihat NN, Fajriutami T, Adi DTN, Fatriasari W, Hermiati E. 2017.
Reducing sugar production of sweet sorghum bagasse kraft pulp. AIP
86
Conference Proceedings Vol. 1803 International Symposium on

Applied Chemistry (ISAC) 2016. Tangerang, Banten, Indonesia, 3-5
Oktober 2016, pp. 020012-1 - 020012-8.
2013
Hermiati E, Risanto L, Lubis MAR, Laksana RPB, Dewi AR. 2017.
Chemical characterization of lignin from kraft pulping black liquor of
Acacia mangium. AIP Conference Proceedings Vol. 1803 International
2012
Symposium on Applied Chemistry (ISAC) 2016. Tangerang, Banten,
Indonesia, 3-5 Oktober 2016, pp. 020005-1 - 020005-7.
Hermiati E, Tsubaki S, Azuma J. 2014. Cassava pulp hydrolysis under
microwave irradiation with oxalic acid catalyst for ethanol
production. Journal of Mathematical and Fundamental Science 46(2):
125-139.
Risanto L, Hermiati E, Sudiyani Y. 2014. Properties of lignin from oil
palm empty fruit bunch and its application for wood adhesive.
Makara Seri Teknologi 18(2): 67-75.
Hermiati E, Anita SH, Risanto L, Styarini D, Sudiyani Y, Hanafi A,
Abimanyu H. 2013. Biological pretreatment of oil palm frond fiber
using white-rot fungi for enzymatic saccharification. Makara Seri
Teknologi 17(1): 39-43.
Hermiati E, Mangunwidjaja D, Sunarti TC, Suparno O, Prasetya B,
Anita SH, Risanto L. 2012. Ethanol fermentation of microwave-
assisted acid hydrolysate of cassava pulp with Saccharomyces
cerevisiae in the presence of activated carbon. International Journal
of Environment and Bioenergy 3(1): 12-24.
Hermiati E, Azuma J, Tsubaki S, Mangunwidjaja D, Sunarti TC, Suparno
O, Prasetya B. 2012. Improvement of microwave-assisted hydrolysis
of cassava pulp and tapioca flour by addition of activated carbon.
Carbohydrate Polymers 87: 939-942.
Hermiati E, Mangunwidjaja D, Sunarti TC, Suparno O, Prasetya B.
2012. Potential utilization of cassava pulp for ethanol production in
Indonesia. Scientific Research and Essays 7(2): 100-106.
87
Xylitol and Biosurfactant from Sugarcane Trash
Euis Hermiati1), Widya Fatriasari1), Maulida Oktaviani1), Dian Burhani1), Riska

Surya Ningrum1), Raden Permana Budi Laksana1), Ahmad Thontowi2), Lutfi
Nia Kholida2), Muhammad Ghozali3), and Takashi Watanabe4)
1)
Research Center for Biomaterials, LIPI, Cibinong, Bogor, Indonesia
2)
Research Center for Biotechnology, LIPI, Cibinong, Bogor, Indonesia
2)
Research Center for Chemistry, LIPI, Serpong, Tangerang Selatan, Indonesia
4)
Kyoto University, Uji, Kyoto 611-0011, Japan
Abstract
Applying biorefinery concept is important in order to increase the

efficiency of the production of second-generation bioethanol. Each
component of lignocellulosic biomass is used to produce fuels and value-
added materials or chemicals. In this e-ASIA project we focus on the
utilization of xylose and lignin from sugarcane trash (ST) for production of
xylitol and biosurfactant, respectively. Research on the utilization of xylose
for the production of xylitol is focused on selecting yeast strain from the final
short list for fermenting xylose to xylitol, and development of xylose
fermentation through evolutionary adaptation of the yeast cells and
immobilization of yeast cells on immobilization matrices, development of
xylitol purification and crystallization. Research on the utilization of lignin for
biosurfactant is focused on the development of biosurfactant synthesis from
ST lignin through grafting with hydrophilic polymers, such as polyvinyl
alcohol, and on evaluation of the effects of the biosurfactant on the
enzymatic hydrolysis of lignocellulosic biomass. The research was started in
April 2019 and will be conducted in three years. We would like to report the
results that we obtained so far within this year. Results of study on
fermentation of xylose in the hydrolysate of acid pretreated sugarcane trash
show that the adapted yeast (Candida tropicalis InaCC Y799) worked faster
and produced higher xylitol (55% of theoretical yield) than the non-adapted
yeast (50.5% of theoretical yield). The study on the immobilization of yeast
cells on calcium alginate matrix for fermentation of xylose to xylitol is still in
progress. Lignin for preparing biosurfactant was isolated by acid precipitation
88
of alkali pretreatment black liquor of sugarcane trash. Results of analyses on

the molecular weight, functional groups, and surface tension of lignin-based
biosurfactant synthesized in this study show that commercial lignin could be
successfully grafted with polyvinyl alcohol. The similar experiment on grafting
of lignin isolated from sugarcane trash is still in progress.
Key words: biomass fractionation, biorefinery, biosurfactant, lignin, xylitol,

xylose
89
Pattanop Kanokratana
Enzyme Technology Laboratory
Biorefinery and Bioproduct Technology Research

Group
National Center for Genetic Engineering and

Biotechnology
113 Thailand Science Park, Phahonyothin Rd.,
Khlong Luang, Pathum Thani 12120, Thailand

Office Tel : +66-2564-6700
Office Fax : +66-2564-6707
Email: pattanopk@biotec.or.th
FIELD OF RESEARCH
Enzyme technology, Metagenomic technology
EDUCATION
2008 – 2013 Doctor of Molecular Genetics and Genetic Engineering,
Institute of Molecular Bioscience,
Mahidol University, Thailand
2000 – 2002 Master of Biotechnology, School of Biotechnology and

Biomolecular Sciences,
University of New South Wales, Australia
1995 – 1999 Bachelor in Food Technology, Faculty of Science,

Chulalongkorn University, Thailand
90
PUBLICATION
2019
1. Champreda V., Mhuantong W., Lekakarn H., Bunterngsook B.,
Kanokratana P., Zhao X.Q., Zhang F., Inoue H., Fujii T.,
Eurwilaichitr L. (2019) Designing cellulolytic enzyme systems for
biorefinery: From nature to application. J Biosci Bioeng. doi:
10.1016/j.jbiosc.2019.05.007.
2. Todhanakasem T., Salangsing OL., Koomphongse P., Kaewket S.,
Kanokratana P., Champreda V. (2019) Zymomonas mobilis Biofilm
Reactor for Ethanol Production Using Rice Straw Hydrolysate
Under Continuous and Repeated Batch Processes. Front
Microbiol. 10: 1777
3. Mangkorn N., Kanokratana P., Roongsawang N., Laobuthee A.,
Laosiripojana N., Champreda V. (2019) Synthesis and
2018
characterization of Ogataea thermomethanolica alcohol oxidase
immobilized on barium ferrite magnetic microparticles. J Biosci
Bioeng. 127: 265-272
4. Todhanakasem T., Sowatad A., Kanokratana P., Havanapan P.-O.,
Champreda V. (2019) Expression and Extracellular Secretion of
Endo-glucanase and Xylanase by Zymomonas mobilis. Applied
Biochemistry and Biotechnology. 187: 239-252. DOI:
10.1007/s12010-018-2821-4
5. Mangkorn N., Kanokratana P., Roongsawang N., Laosiripojana N.,
Champreda V. (2018) Purification, characterization, and
stabilization of alcohol oxidase from Ogataea
thermomethanolica. Protein Expression and Purification. 150: 26-
32
6. Phetcharat T., Dawkrajai P., Chitov T., Wongpornchai P., Saenton
S., Mhuantong W., Kanokratana P., Champreda V., Bovonsombut
S. (2018) Effect of inorganic nutrients on bacterial community
composition in oil-bearing sandstones from the subsurface strata
of an onshore oil reservoir and its potential use in Microbial
2016
Enhanced Oil Recovery. PLoS One. 13 (11): e0198050
7. Aurepatipan N., Champreda V., Kanokratana P., Chitov T.,
Bovonsombut S. (2018) Assessment of bacterial communities and
2015
activities of thermotolerant enzymes produced by bacteria
91
indigenous to oil-bearing sandstone cores for potential

application in Enhanced Oil Recovery. J Petr Sci Eng. 163: 295-302
8. Kanokratana P., Wongwilaiwalin S., Mhuantong W.,
Tangphatsornruang S., Eurwilaichitr L., Champreda V. (2018)
2013 Characterization of cellulolytic microbial consortium enriched on
Napier grass using metagenomic approaches. J Biosci Bioeng. 125:
439-447.
2011 9. Todhanakasem T., Yodsanga S., Sowatad A., Kanokratana P.,
Thanonkeo P., and Champreda V. (2018) Inhibition analysis of
inhibitors derived from lignocellulose pretreatment on the
2011 metabolic activity of Zymomonas mobilis biofilm and planktonic
cells and the proteomic responses. Biotechnol Bioeng. 115: 70-81.
10. Kanokratana P., Mhuanthong W., Laothanachareon T.,
Tangphatsornruang S., Eurwilaichitr L., Kruetreepradit T., Mayes
2010 S., Champreda V. (2016) Comparative study of bacterial
communities in Nepenthes pitchers and their correlation to
species and fluid acidity. Microb Ecol. 72: 381-393.
11. Mhuantong W., Charoensawan V., Kanokratana P.,
2008 Tangphatsornruang S., Champreda V. (2015) Comparative analysis
of sugarcane bagasse metagenome reveals unique and conserved
biomass-degrading enzymes among lignocellulolytic microbial
2007 communities. Biotechnol Biofuels. 8: 16.
12. Kanokratana P., Eurwilaichitr L., Pootanakit K., Champreda V.
(2015) Identification of glycosyl hydrolases from a metagenomic
library of microflora in sugarcane bagasse collection site and their
2006 cooperative action on cellulose degradation. J Biosci Bioeng. 119:
384-391.
13. Kanokratana P., Mhuanthong W., Laothanachareon T.,
Tangphatsornruang S., Eurwilaichitr L., Pootanakit K., Champreda
V. (2013) Phylogenetic analysis and metabolic potential of
microbial communities in an industrial bagasse collection site.
Microb. Ecol. 66: 322–334.
2004 14. Rattanachomsri U., Kanokratana P., Eurwilaichitr L., Igarashi Y.,
Champreda V. (2011) Culture-independent phylogenetic analysis
of the microbial community in industrial sugarcane bagasse
feedstock piles. Biosci. Biotechnol. Biochem. 75: 232-239.
92
15. Kanokratana P., Uengwetwanit T., Rattanachomsri U.,

Bunterngsook B., Nimchua T., Tangphatsornruang S.,
Plengvidhaya V., Champreda V., Eurwilaichitr L. (2011)
Metagenomic analysis on phylogenetics and metabolic potential
of microbial community in primary tropical peat swamp forest.
Microb. Ecol. 61: 518-528.
16. Bunterngsook B., Kanokratana P., Thongaram T., Tanapongpipat
S., Uengwetwanit T., Rachdawong S., Vichitsoonthonkul T., and
Eurwilaichitr L. (2010) Identification and characterization of
lipolytic enzymes from a peat-swamp forest soil metagenome.
Biosci. Biotechnol. Biochem. 74: 1848-1854.
17. Kanokratana P., Chantasingh D., Champreda V., Tanapongpipat S.,
Pootanakit K. and Eurwilaichitr L. (2008) Identification and
expression of cellobiohydrolase (CBHI) gene from an endophytic
fungus, Fusicoccum sp. BCC4124 in Pichia pastoris. Protein Expr.
Purif. 58: 148-153.
18. Champreda V., Kanokratana P., Sriprang S., Tanapongpipat S. and
Eurwilaichitr L. (2007) Purification, biochemical characterization
and gene cloning of a new extracellular thermotolerant and
glucose tolerant maltooligosaccharide-forming -amylase from
an endophytic ascomycete Fusicoccum sp. BCC4124. Biosci.
Biotechnol. Biochem. 71: 2010-2020.
19. Tang K., Utairungsee T., Kanokratana P., Sriprang R., Champreda
V., Eurwilaichitr L., and Tanapongpipat S. (2006) Characterization
of a novel cyclomaltodextrinase expressed from environmental
DNA isolated from Bor Khleung hot spring in Thailand. FEMS
Microbiol. Lett. 260: 91-99.
20. Chantasingh D., Pootanakit K., Champreda V., Kanokratana P.,
and Eurwilaichitr L. (2006) Cloning, expression and
characterization of a xylanase 10 from Aspergillus terreus
(BCC129) in Pichia pastoris. Protein Expr. Purif. 46: 143-149
21. Kanokratana P., Chanapan S., Pootanakit K., and Eurwilaichitr.
(2004) Diversity and abundance of Bacteria and Archaea in the Bor
Khlueng Hot Spring in Thailand. J. Basic Microbiol. 44: 430-444.
93
Construction of cellulolytic P. pastoris containing iso-butanol synthesis

pathway for partial consolidated bioprocessing of sugarcane trash
Weerawat Runguphan, Benjarat Bunterngsook, Pattanop Kanokratana, and
Verawat Champreda
Biorefinery and Bioproduct Technology Research Group, National Center for
Genetic Engineering and Biotechnology, Pathum Thani, Thailand
Abstract
Sugarcane trash (ST) defined as the left tops and leaves after
harvesting is a promising underused resource in Southeast Asia. Its availability
is expected to increase after restriction of field blaze to alleviate the air
pollution problems. In this e-Asia project, conversion of ST to multi-products
based on zero-waste with multi-disciplinary technologies will be explored to
transform the existing sugar industry to modern biorefineries. On Thai-side,
utilization of the cellulose-enriched fraction to iso-butanol will be studied. An
engineered Pichia pastoris has been constructed with an iso-butanol
synthesis pathway by incorporating 6 genes—4 from the endogenous valine
amino acid biosynthesis and 2 from the exogenous keto-acid degradation
pathway—into the genome. This led to the maximum iso-butanol titer and
yield of 2.2 g/L and 22 mg/g glucose, respectively. Selected genes encoding
for cellulolytic enzymes, including Cel12 from Aspergillus aculeatus
BCC17849, Xyn10 from Aspergillus aculeatus BCC17849, BGAA from
Aspergillus aculeatus, Cel7BTT from Thielavia terrestris, and Cel6ACT from
Cheatomium thermophilum were integrated into the iso-butanol producing
strain in order to develop a strain for partial consolidated bioprocessing (CBP)
of the pretreated ST. Engineered strains were able to produce up to 54.5 mg/L
iso-butanol directly from pretreated sugarcane bagasse. Metabolic flux of the
iso-butanol synthesizing yeast will be further studied and fine-tuned through
collaboration with Kyoto University in the e-Asia project. Bioprocessing of ST
to iso-butanol using the engineered strain will be further explored. The work
will provide an alternative strategy for valorization of ST in biorefineries.
Key words (4-6 keywords 10.5p bold): Sugarcane trash, Consolidated
bioprocessing, Iso-butanol, Cellulolytic, Pichia pastoris
94
Chartchai Khanongnuch
Division of Biotechnology
Faculty of Agro-Industry, Chiang Mai University
155 Moo 2, T. Maehia, A Meuang, Chiang Mai
Thailand 50100
Office Tel : +66-53 948261
Office Fax : +66-53 948206
Home Tel : +66-89 755-9045
Email: chartchai.k@cmu.ac.th
FIELD OF RESEARCH
Applied Microbiology and Biochemistry
EDUCATION
1994 – 1999 Ph.D. (Molecular Chemistry)
Graduate School of Engineering, Hokkaido University, Japan
1989 – 1992 M.Sc. (Biology), Department of Biology, Faculty of Science,
Chiang Mai University, Thailand
1982 – 1986 B.Sc. (Radiological Technology), Associated Medical Science,
Chiang Mai University, Thailand
PUBLICATION
2019
Unban, K., Khatthongngam, N., Shetty, K. and Khanongnuch, C.
“Nutritional biotransformation in traditional fermented tea (Miang)
from north Thailand and its impact on antioxidant and antimicrobial
activities” Journal of Food Science and Technology. 56(6): 2687-2699
2019
(2019).
Kaprasob, R., Sarkar, D., Kerdchoechuen, O., Laohakunjit, N.,
Khanongnuch, C., Shetty, K. “Beneficial lactic acid bacteria
based bioprocessing of cashew apple juice for targeting antioxidant
2019
95
nutraceutical inhibitors as relevant antidotes to type 2 diabetes”

Process Biochemistry. 82: 40-50 (2019).
Unban, K., Kanpiengjai, A., Khatthongngam, N., Saenjum, C. and
2019 Khanongnuch, C. “Simultaneous bioconversion of gelatinized starchy
waste from the rice noodle manufacturing process to lactic acid and
maltose-forming α-amylase by Lactobacillus plantarum S21, using a
2018 low-cost medium. Fermentation. 5: 32
Kanpiengjai, A., Unban, K., Nguyen, T.H., Haltrich, D. and
Khanongnuch, C. “Expression and biochemical characterization of a
new alkaline tannase from Lactobacillus pentosus” Protein
2018 Expression and Purification 157: 36-41 (2019)
Doan, H., Hoseinifar, S.H., Khanongnuch, C., Kanpiengjai, A., Unban,
K., Van Kim, V. and Srichaiyo, S. “Host-associated probiotics boosted
mucosal and serum immunity, disease resistance and growth
2018 performance of Nile tilapia (Oreochromis niloticus)” Aquaculture
491: 94-100 (2018)
Penkhrue, W., Sujarit, K., Kudo, T., Ohkuma, M., Masaki, K., Aizawa,
2017 T., Pathom-aree, W., Khanongnuch, C. and Lumyong, S.
“Amycolatopsis oliviviridis sp. Nov., a novel polylactic acidbioplastic-
degrading actinomycete isolated from paddy soil” International
Journal of Systematic and Evolutionary Microbiology 68: 1448-1454
(2018)
2017 Kanpiengjai, A., Mahawan, R., Pengnoi, P., Lumyong, S. and
Khanongnuch, C. “Improving the monacolin K to citrinin production
ratio in red yeast rice by an X-ray-induced mutant strain of
2017 Monascus purpureus” Bio Technologia 99(2): 109-118 (2018)
Unban, K., Kanpiengjai, A., Lumyong, S., Nguyen, T.H., Haltrich, D.
and Khanongnuch, C. “Molecular structure of cyclomaltodextrinase
2017 derived from amylolytic lactic acid bacterium Enterococcus faecium
K-1 and properties of recombinant enzymes expressed in Escherichia
coli and Lactobacillus plantarum” International Journal of Biological
2017 Macromolecules 107: 898-905 (2018)
96
Noisuwan, P., Takata, G., Uechi, K., Khanongnuch, C., Pathom-aree,

W., and Lumyong, S. “Optimization of Mycodextran Production from
Efficient Fungus in Submerged Culture” Chiang Mai Journal of
2017 Science 44(4): 1231-1243 (2017)
Khanongnuch, C., Kanpiengjai, A., Unban, K., and Saenjum, C.
“Recent Research Advances and Ethno-Botanical History of Miang, a
Traditional Fermented Tea (Camellia sinensis var. assamica) of
2017 Northern Thailand” Journal of Ethnic Foods. 4: 135-144 (2017)
Chaikaew, C., Kanpiengjai, A., and Khanongnuch, C. Diversity of
lactic acid bacteria from Miang, a traditional fermented tea leaf in
northern Thailand and their tannin-tolerant ability in tea extract.
2017 Journal of Microbiology. 55(9): 720-729 (2017)
Kanpiengjai, A., Nguyen, TH., Haltrich, D., and Khanongnuch, C.
Expression and Comparative Characterization of Complete and C-
2016 terminally Truncated Forms of Saccharifying α-Amylase from
Lactobacillus plantarum S21. International journal of biological
macromolecules. 103: 1294-1301 (2017)
Penkhrue, W., Kanpiengjai, A., Khanongnuch, C., Masaki, K., Pathom-
aree, W., Punyodom, W. and Lumyong. S. “Effective enhancement of
polylactic acid (PLA)-degrading enzyme production by Amycolatopsis
sp. strain SCM_MK2-4 using statistical and one-factor-at-a-time
(OFAT) approaches” Preparative Biochemistry & Biotechnology 47(7):
730-738 (2017).
Pengnoi, P., Mahawan R., Khanongnuch, C. and Lumyong, S.,
“Antioxidant Properties and Production of Monacolin K, Citrinin, and
Red Pigments during Solid State Fermentation of Purple Rice (Oryzae
sativa) Varieties by Monascus purpureus” Czech Journal of Food
Sciences 35: 32-39 (2017).
Chaikaew, C., Kanpiengjai, A., Intatep, J., Unban, K., Takata, G. and
Khanongnuch, C. “X-Ray-Induced Mutation of Bacillus sp. MR10 for
Manno-Oligosaccharides Production from Copra Meal” Preparative
Biochemistry & Biotechnology 47: 424-433 (2017)
97
Kanpiengjai, A. Chui-Chai, N., Chaikaew, S. and Khanongnuch, C.

“Distribution of tannin-'tolerant yeasts isolated from Miang, a
traditional fermented tea leaf (Camellia sinensis var. assamica) in
northern Thailand” International Journal of Food Microbiology 238:
121-131 (2016).
98
Optically pure L-lactic acid production from lignocelluloses
Chartchai Khanongnuch1* Pannita Pamuangmool Augchararat Klongklaew

Kridsada Unban and Takashi Watanabe2
1
Division of Biotechnology, Faculty of Agro-Industry, Chiang Mai University,
Maehia, Meuang Chiang Mai, 50100, Thailand
2
*Corresponding author: chartchai.k@cmu.ac.th
Abstract. Poly lactic acid (PLA) has been developed as a renewable alternative
for petroleum-plastic using the polymerization of optically pure L- or D-lactic
acid. Most of optically pure L- or D-lactic acid are produced by microbial
fermentation process using fermentable sugar from various sources as
substrates. Regarding food security reason, using sugars derived from food
sources is not appropriate, lignocellulosic substrate is then becoming the
target. Corn stover, a residue from corn production which is recently caused
the problem of air pollution in northern Thailand, is targeted to be used for L-
lactic acid production. The preliminary study on lactic acid production by
mixed Lactobacillus rhamnosus SCJ9 and Enterococcus mundtii WX1 was
investigated using the glucose and xylose mixed sugar (6:4 ratio). Among 9
medium components, Plackett and Burman design and central composite
design (CCD) predicted the maximum lactic acid yield 18.84 g/L from 20 g/L
carbon source using g/L of yeast extract 24.2, Tween80 9.8 and MnSO 4 11.8.
Lactic acid production in 5-L fermenter by WX1:SCJ9; (1:1) in MRS at 37°C for
24 h. Lactic acid yield reach 0.76 g/g at 48 h with high productivity of 2.8 g/L/h
at 6 h. The fermentation of corn stover derived sugars was also demonstrated
the high potential.
Keywords : bioplastics, biorefinery, lactic acid, lignocelluloses, corn stover
99
Oral Presentation
100
FOREST
SCIENCE
101
The Relatioship of Dimension and Ampel Bamboo

Geometry on Its Modulus of Elasticity
Kartini1*, Naresworo Nugroho2, Effendi Tri Bahtiar2
1,2Faculty of Forestry, IPB University, Kampus IPB Dramaga-Bogor, West Java,

Indonesia 16680
*Corresponding author: kartini_nyo@apps.ipb.ac.id
Abstract. Traditionally, Indonesian people have used bamboo as a

construction material. Whole bamboo stems are called bamboo culm which
are round, hollow, have several segments and tapered. Regarding to the
development of bamboo utilization for construction material, testing
standards and code of practices are needed to ensure the safety of the users.
The quality of bamboo construction can be improve by using stress grading
system. Therefore, this research needs to obtain the grading variable that
influence modulus of elasticity of Bambusa vulgaris. Based on analysis of their
variable, bamboo's dimension and geometry are not affected to modulus
elasticity value. This result shows tha the further research for grading system
is needed to obtain variables of Bambusa vulgaris that correlate with the
modulus of elasticity
Keywords : Bamboo culm; stress grading system;, modulus of elasticity
102
Study on lignocellulose degradation by wood

borer beetles, Heterobostrychus aequalis and
Dinoderus minutus
Didi Tarmadi1*, Yuki Tobimatsu2, Maya Ismayati1, Takuji Miyamoto2, Andri Fadillah
Martin2,3, Titik Kartika1, S Khoirul Himmi1, Ikhsan Guswenrivo1, Deni Zulfiana1,
Sulaeman Yusuf1, Tsuyoshi Yoshimura2, Toshiaki Umezawa2
1Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI), Jl. Raya
Bogor KM.46, Cibinong, Bogor, West Java, 16911, Indonesia.
2Research Institute for Sustainable Humanosphere (RISH), Kyoto University, Gokasho
Uji, Kyoto 611-0011, Japan.

3Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya
Bogor KM.46, Cibinong, Bogor, West Java, 16911, Indonesia.

*Corresponding author: didi@biomaterial.lipi.go.id
Abstract. To understand how wood borer beetles decompose lignocellulose

components for their metabolism, we analyzed undigested lignocelluloses and
resulted frass (excretion products) samples derived from infested rubber
wood by Heterobostrychus aequalis and infested bamboo by Dinoderus
minutus using wet chemistry and NMR spectroscopy. The results showed
there was no significant different between content of lignocellulose
components (cellulose, hemicellulose, and lignin) in frass and those in
undigested lignocellulose samples for both rubber wood and bamboo
samples, suggesting that H. aequalis and D. minutus not utilize cellulose,
hemicelluloses, and lignin for their metabolism. Regardless the lignocellulose
samples source, we detected there was significant different between starch
content in frass and in undigested lignocellulose samples. The starch content
in frass was significantly lower than that in undigested lignocellulose samples.
This result clearly suggests that H. aequalis and D. minutus utilize starch for
their metabolism. Overall, our data support the view that wood borer beetles
decompose starch as their main food source.
Keyword: wood-borer beetles, lignocellulose, wet chemistry, NMR

spectroscopy
103
Mapping of Parkia speciosa land suitability

distribution as one of Multy Purpose Tree Species at
community agroforestry land
Rahmawaty1*, Ruth Maria Eltiana Marpaung1, Abdul Rauf2, Ridwanti Batubara3
1Department of Forest Management, Faculty of Forestry, Universitas Sumatera Utara,

Jl. Tridharma Ujung No.1, Kampus USU Medan, Sumatera Utara, Indonesia 20155
2Faculty of Agriculture, Universitas Sumatera Utara, Medan, Sumatera Utara
Indonesia 20155
3Department of Forest Product Technology, Faculty of Forestry, Universitas Sumatera
Utara, Jl. Tridharma Ujung No.1, Kampus USU Medan, Sumatera Utara, Indonesia
20155
*Corresponding author: rahmawaty@usu.ac.id
Abstract. Parkia speciose is one of Multy Purpose Tree Species (MPTS),

belongs to the family Fabaceae. It commonly found in agroforestry land in
Langkat District, North Sumatra. This study aimed to map the distribution of
land suitability for Parkia speciosa in Peria-ria Village and Sari Laba Jahe
Village, Biru-biru Sub District, Deli Serdang District, North Sumatra, Indonesia.
This research was conducted by survey method to collect soil samples based
on land unit. Land unit was obtained from overlay soil map, land use map and
slope map. Land suitability was evaluated based on the matching method. The
Geographic Information System (GIS) was used to map the distribution of land
suitability class. The results showed that the actual land suitability class for
Parkia speciose were moderately suitable (S2) (8.25%), marginal suitable (S3)
(14.26%) and Not suitable (N) (77.5%) in this area. Erosion hazard (eh), root
zone medium (rc), availability of water (wa), and nutrient retention (nr) were
the dominant of limiting factor in this area. The most difficult constrain to
counter were water availability and root zone medium.
Keywords : agroforestry, community, land suitability, Parkia speciose
104
Community Forest for Increasing Transtition Zone

(Suburban) Environment: Water Infiltration and
Fresh Air
Resti Kharisma1*
1ForestManagement, Faculty Of Forestry,

IPB University, Dramaga-Bogor 16680, Indonesia
*Corresponding author: restikharisma@mail.com
Abstract. The last few decades, land use change was one of main topics of the
research. Land use change was often associated with changing a function of
land into other functions. Generally found, agricultural or forest area became
built area, caused the expansion of the city or infrastructure development.
Surely, it increased run off and other impacts. This situation usually occurs at
the transition zone (suburban), producing new issue about environment.
Especially, Depok and Bogor commonly found shrubs or unproductive land
that can be transformed into productive land for example community forest.
In addition it can decrease surface water and provide fresh air greater than
shrubs. Research goal is to apply the concept of community forest for
unproductivity land. The expected results that this concept can be one of the
paradigm for land management and have a major impact particularly for the
environment.
Keywords : Community forest; land use change; transition zone
105
Development of Identification System for

Limited Wood Species
Tri Broto Sisworo1, Esa Prakarsa2, Setyorini1
1Facultyof Informatics, Telkom University, Jl. Telekomunikasi, Terusan Buah Batu,

Sukapura, Kec. Dayeuhkolot, Bandung, Jawa Barat 40257
2Research Center of Informatics, Lipi, Komp. LIPI Gedung 20 Lt. 3, Jl. Sangkuriang,
Dago, Kec. Coblong, Bandung, Jawa Barat 40135
Corresponding author: tribrotosiswoyo@student.telkomuniversity.ac.id,

esa.prakasa@lipi.go.id and setyorini@telkomuniversity.ac.id@lipi.go.id
Abstract. Identification of wood species is needed when buying / selling wood

and calculation of taxes when exporting or importing wood, usually in the
identification of wood species requires a relatively long time so that hamper
business processes. So needed a system that can identify wood species
quickly. In this research, a system was built that could identify 10 species of
wood using the Resnet50 architecture in Deep Learning. The system built has
an accuracy of 90.69%.
Keywords : wood; species; deep learning; resnet50
106
BIOSCIENCE
107
Effect of Artocarpus heterophyllus Leaf Ethanol

Extract on Skin Smoothness
T Widyawati1*, S Syarifah1, M Daulay2 and L F Mustanti3
1Department of Pharmacology and Theraputic, Faculty of Medicine, Universitas

Sumatera Utara, Medan, 20155, Indonesia
2Department of Physiology, Faculty of Medicine, Universitas Sumatera Utara, Medan,
20155, Indonesia
3Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia
*Corresponding author: tw_rozan@yahoo.com/tri.widyawati@usu.ac.id
Abstract. Artocarpus heterophyllus (Ah) is a plant that grows in many parts of

Indonesia. Its leaves are widely used for traditional uses, one of which is for
facial skin care. The present study was conducted to evaluate the effect of its
extract on facial skin smoothness. Ethanol extract of Ah (EEA) was prepared as
clay mask formulation (F1: 1, F2: 3 and F3:5%) and then were applied on
volunteer face. Skin smoothness improvement was measured using skin
analyzer. Standardization of simplicia and phytochemical screening of EEA
were done before the test. Data were analysed by Wilcoxon Signed Ranks Tes
using SPSS 22. Standardization yielded as follows: total water : 5.33%, total
water soluble 15.67%, total ethanol soluble 13.61% and total ash 10.29%.
Phytochemical screening identified the presence of tannin, saponin, flavonoid
and glicosyde. Smoothness value showed as normal skin with improvement to
smooth level on EEA-treated group: F1: 45-50 to 39-49; F2: 41- 44 to 37- 42;
F3: 39-41 to 35-37. The highest improvement persentage of skin was found in
F3 (10.0%). However, statistically, there was no significant different (p>0.05)
before and after EEA treatment. The study suggests that evaluation should be
carried out longer for up to 4 weeks.
Keywords : Artocarpus heterophyllus; extract ; skin smoothness
108
Evaluation of Myrmecodia pendans Water Extract on

Blood Glucose Level and Liver Function in Diabetic
Patients
Tri Widyawati1*, M.Aron Pase 2, Milahayati Daulay 3 and Imam Bagus Sumantri 4
1Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas
Sumatera Utara, Medan, 20155, Indonesia

2Department of Internal Medicine, Faculty of Medicine, Universitas Sumatera
Utara, Medan, 20155,

3Department of Physiology, Faculty of Medicine, Universitas Sumatera Utara,
Medan, 20155, Indonesia; milahayatidaulay@usu.ac.id

4Department of Pharmacy Biology, Faculty of Pharmacy, Universitas Sumatera
Utara, Medan, 20222, Indonesiand
*Corresponding author: tri.widyawati@usu.ac.id/tw_rozan@yahoo.com
Abstract. Previous study showed the safety and antioxidant activity of

Myrmecodia pendans (M.pendans) water extract (MPWE)dose 350 mg in
healthy human. This study was addressed to evaluate its effect on blood
glucose level (fasting (FBGL) and post prandial (BGLpp)) and liver function of
diabetic patients. Type 2 diabetes mellitus patients were randomized to begin,
a 14-day treatment of double-blind MPWE (n=4) versus placebo (Pl)(n = 4) 350
mg once daily. Data were analysed using independent t-test (Mann Whitney)
and Wilcoxon sign test. FBGL (mg/dL) and ppBGL (mg/dL) of MPWE-group
showed the trend to decrease (8.99% and 9.75%): FBGL from (158 - 352) to
(207 - 235) and ppBGL from (382 - 469) to (344 - 423). In Pl-group, FBGL
increased from (213 - 258) to (219 - 333) while ppBGL decreased (18.3%) from
(355 - 411) to (290 - 329). SGOT and SGPT level (U/L) showed as follows: MPWE
( SGOT: (20.0 - 34.0) - (17.5 - 24.0); SGPT (27.5 - 46.0) - (28.8 - 53.0); Pl (SGOT:
17.0 - 28.0) - (21.5 - 47.0); SGPT: (20.0 - 33.0) - (23.3 - 48.0). Compared to
baseline, statistically, 2 weeks of supplementation resulted in no significant
changes in FBGL, ppBGL, SGOT and SGPT level (p>0.05). This pilot study
concluded that MPWE may have antidiabetic effect by increasing the dose
and/or prolong the treatment.
Keywords : blood glucose, extract, liver, Myrmecodia pendans, extract,

diabetes
109
The Superiority of Chitosan in Promoting

Micropropagation of a Dendrobium Hybrid Orchid
Yeow Lit Chow1, Chew Bee Lynn2 and Sreeramanan Subramaniam3*
1,2,3School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang,
Malaysia.
*Corresponding author: sreeramanan@gmail.com
Abstract. Chitosan is a biomaterial formed as a result of chitin deacetylation.

This natural linear polysaccharide possesses biocompatible, biodegradable,
and mucoadhesive properties. Present study explored the effects of various
plant growth regulators and chitosan in promoting micropropagation of
Dendrobium orchid hybrid (Dendrobium Enopi x Dendrobium Pink Lady). In this
study, combination of 6-benzylaminopurine (BAP) and 1-naphthaleneacetic
acid (NAA) at 0 (control), 0.5, 1.0 and 2.0 mg/L, as well as the incorporation of
singly applied exogenous hormones, indole-3-butyric-acid (IBA), kinetin, 2,4-
dicholorophenoxyacetic acid (2,4-D) at 0 (control), 0.5, 1.0, 1.5, 2.0, 2.5 and
3.0 mg/L shown no significant effects in enhancing in vitro protocorm-like
bodies (PLBs) proliferation, shooting and rooting capacities. However, the
incorporation of 0.50 mg/L chitosan stimulated the highest PLBs fresh weight
of 0.9516 g. The highest shoot formation rate at 60th day was obtained using
0.25 mg/L chitosan with an average of 15.69 shoots. In addition, an average
length of 0.199 cm was recorded and further elevated to 25.29 shoots with
average length of 0.3167 cm during 100th day using 0.25 mg/L chitosan.
Furthermore, chitosan at the concentration of 0.25 mg/L induced significantly
higher root number (5.15) and the longest root length (0.506 cm) among all
the other tested treatments.
Keywords : Micropropagation, plant growth regulators, chitosan, in vitro,

protocorm-like bodies
110
Comparison of Cuticular Thickness between Two

Insecticide Resistant Strains of Tropical Bed Bug,
Cimex hemipterus F. (Hemiptera: Cimicidae)
Li-Shen Soh¹, Chow-Yang Lee ², G. Veera Singham¹*
1Centre for Chemical Biology, Universiti Sains Malaysia, Bayan Lepas, Penang, Malaysia
2Department of Entomology, University of California, Riverside, CA 92521, USA
*Corresponding author: veerasingham@usm.my
Abstract. Inadequate management approaches of insecticide caused the bed

bugs to develop resistance towards insecticides and consequently leads to bed
bug resurgence. Reduced cuticular penetration has been correlated with
insecticide resistance development. It was proven that individuals with thicker
cuticle within the population showed higher insecticide resistance. Present
study aims to evaluate the role of cuticular thickness of two populations with
similar pyrethroid resistance level, on insecticide resistance development. In
this study, cuticle thickness of bed bugs from two populations collected from
Malaysia (MAS-1) and Australia (AUS-1) were measured while toxicity
bioassays were conducted using insecticides pyrethroid and organophosphate
in order to evaluate the presence of resistance due to cuticular penetration.
Microscopic analysis showed that cuticle layers of MAS-1 (8.613 μm) were
significantly thicker than that of AUS-1 strain (6.326 μm). Both strains
displayed no significant difference in terms of knockdown time towards
pyrethroid, where both exceeded 72 hours. For organophosphate, KT50/KT99
(time to knockdown 50%/99% of the population) values were recorded at
556.52/986.27 minutes and 660.42/1003.57 minutes for AUS-1 and MAS-1
strains respectively. Present study demonstrated that thickened cuticle plays
a significant role in bed bugs resistance towards the tested insecticides,
however, the effectiveness tends to reduce by time.
Keywords : insecticide resistance,cuticle, pyrethroid, organophosphate, bed

bug
111
Analysis of Passion Fruit Seed’s Ethanol

Dina Keumala Sari1*, Marianne2, Sri Lestari3
1Nutrition Department, Faculty of Medicine, Universitas Sumatera Utara

2Pharmacology Pharmacy Department, Faculty of Pharmacy, Universitas Sumatera
Utara
3Public Health Department, Faculty of Medicine, Universitas Sumatera Utara
*Corresponding author: dina@usu.ac.id
Abstract. High oxidative stress in cells due to inflammation process or

excessive cell proliferation would produce oxidants or free radicals with
biomarkers, one of which is malondialdehyde (MDA). Aside from that, high fat
consumption would increase lipoprotein level in circulation which would
trigger atherogenesis process. Passion fruit (Passiflora edulis Sims) seeds
contain high antioxidant and are expected to decrease the level of cholesterol
and MDA. The objective of this study is to identify the effect of passion fruit
seed’s ethanol extract in Wistar rats that have been fed with atherogenic feed.
The method of this study was to find out the flavonoid and phytochemical
screening. Flavonoid total content: 23.43±2.31 mgQE/extract gram and Fenol
total content: 497.52±17.21 GAE/extract gram.The results of this study
indicate that antioxidant activity has a correlation with flavonoid levels, DPPH
capture activity by passion fruit seed extract showed a very strong relationship
between the content of polyphenols and free radical capture activities.
Keywords: antioxidant; atherogenegesis; ethanol; flavonoid, passiflora edulis
112
Vitamin D deficiency, vitamin A, beta carotene and

other parameter in tuberculosis patients
Dina Keumala Sari1*, Ridha Dharmajaya2, Mutiara Indah Sari3, Dewi Masyithah4,
Nurfida Khairina Arrasyid4, Ahmad Rafiki5, and Clare Anthony a/p Thomas5
1Department of Nutrition, Faculty of Medicine, Universitas Sumatera Utara,
dina@usu.ac.id
2Department of Neurosurgery, Faculty of Medicine, Universitas Sumatera Utara,
dharmajayaridha@gmail.com
3Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara,
muti_dr@yahoo.com
4Department of Parasitology, Faculty of Medicine, Universitas Sumatera Utara,
dmasyithah57@gmail.com; nurfida@usu.ac.id
5Pre-clerkship Medical Program, Faculty of Medicine, Universitas Sumatera Utara,
ahmad_5050_rafiqi@yahoo.com; clarethomas1994@gmail.com
*Corresponding author: dina@usu.ac.id
Abstract. Nutrition problem have impact in Tuberculosis disease progressivity
especially in Indonesia. Numerous micronutrients have immunomodulatory
roles that may influence risk of tuberculosis, including vitamin and mineral.
The objective of the research was to find out the vitamin A (retinol), vitamin D
(25(OH)D), beta carotene, and calcium serum level in tuberculosis patients.
This research was cross sectional study, with four laboratory parameters using
High Performance Liquid Chromatography (HPLC) for retinol and beta
carotene serum level, chemiluminescent assay for 25(OH)D serum level, and
ADVIA chemistry system for calcium level. Two more parameters were
albumin and blood glucose level. This research including 71 pulmonary TB
patients, in three public medical center in Medan City from April to August
2017. Research result reported mean retinol serum level were 1.50.3 µmol/L,
25(OH)D serum level were 20.36.6 ng/mL, beta carotene serum level were
2.80.9 µmol/L, and calcium serum level were 9.52.3 mg/dL. Based on
category most of the subjects were had micronutrients deficiency except for
calcium, albumin, and blood glucose level, that remains in normal range level.
The conclusions of this research was low vitamin A, vitamin D, and beta
carotene level showed that progressivity of tuberculosis affected the role of
micronutrients but not for calcium. This probably based on malnutrition
condition of tuberculosis patients.
Keywords: albumin; micronutrient; progressivity; sputum conversion;
tuberculosis
113
The Association of GSTM1 Polymorphism with IgA

Level of Nasopharyngeal Carcinoma Patients in
Gunungsitoli
F Farhat1*, M I Sari2, J Chrestella3, R P Syari4
1Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine,

Universitas Sumatera Utara, Medan, Indonesia
Medan, Indonesia
3Department of Anatomic Pathology, Faculty of Medicine, Universitas Sumatera Utara,
Medan, Indonesia
4Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
*Corresponding author: farhat@usu.ac.id
Abstract. Polymorphism of Glutathione S-transferase M1 (GSTM1) increases

the development of nasopharyngeal carcinoma (NPC) due to inactive
functions for detoxification of nitrosamine. The genetic variance causes
accumulation of reactive oxygen species (ROS) and enhances Epstein Barr
Virus (EBV) lytic reactivation, which leads to the increasing of IgA level. This
study aims to identify the relation of GSTM1 polymorphism and IgA level of
Nias Native NPC patients in Gunungsitoli. The study was analytic research with
a cross-sectional design. Samples were blood, and then the polymorphism of
GSTM1 was identified by the PCR-RFLP method, and the IgA level was
measured by ELISA method. There were 29 patients; the male was more
common than the female with a ratio of 1.4:1. WHO type II, which is non-
keratinizing cell carcinoma, was found common than other types with 21
samples (72.4%). There were 20 patients (69.0%) with null polymorphism of
GSTM1 and 21 patients (72.4%) with elevated IgA. Fisher's exact test used to
analyze the association of GSTM1 polymorphism and IgA level and result in the
significant association of those two (p <0.05). This study can be used to identify
the immune biomarker, which could be used as the prognostic marker of NPC
patients in the future.
Keywords: GSTM1, IgA, NPC, polymorphism, Native Nias
114
The Association of GSTM1 Polymorphism with Total

Antioxidant Status of Nasopharyngeal Carcinoma
Patients in Gunungsitoli, Nias
F Farhat1*, M I Sari2, J Chrestella3, R P Syari4

Medan, Indonesia
Medan, Indonesia
Abstract. Abstract. Nasopharyngeal carcinoma (NPC) is developed by the

relation of genetic abnormalities, Epstein Barr Virus (EBV) infection and
environment. Glutathione S-transferase M1 (GSTM1) gene polymorphism
induces NPC by damaging the DNA through the failure of inhibiting oxidative
stress especially in patients with positive risk for environmental risk. The loss
of function lead to decrease antioxidant status of NPC patients. This study
used cross sectional design and was done to evaluate the association of
GSTM1 polymorphism with total antioxidant status (TAS) of NPC patients with
native people of Nias in Gunungsitoli. Samples were blood which underwent
DNA isolation, PCR-RFLP method to identify GSTM1 polymorphism. TAS was
measured by ELISA method. The association of GSTM1 polymorphism and TAS
was analyzed using Fisher’s exact test. There were 29 NPC patients with male
with 17(58.6%) patients and female with 12(41.4%) patients. We found 20
(69.0%) patients with null polymorphism of GSTM1 and 21 patients (72.4%)
with lower TAS. There was significant association of GSTM1 polymorphism and
TAS in NPC. It revealed that GSTM1 gene identification may be used as
indicator of the risk for NPC as well as the additional reason to include
antioxidant intake to prevent NPC especially in patients with GSTM1 null
genotype.
Keywords: GSTM1, NPC, TAS, polymorphism.
115
Polymorphism of CYP2E1 in nasopharyngeal

carcinoma patients in Haji Adam Malik General
Hospital Medan
F Farhat1*, Elvita Rahmi Daulay2, J Chrestella3, R P Syari4

2Department of Radiology, Faculty of Medicine, Universitas Sumatera Utara, Medan,
Indonesia
Medan, Indonesia
Abstract. Genetic is contributed to the development of nasopharyngeal

carcinoma (NPC). CYP2E1 enzyme works by the activation of pro-carcinogens.
The polymorphism of this enzyme leads to the development of NPC. This study
aimed to identify CYP2E1 polymorphism in NPC patients in Haji Adam Malik
General Hospital Medan. The study was descriptive research with a cross-
sectional design. Samples were taken based on non-probability consecutive
sampling method. The identification of CYP2E1 polymorphism was done by the
PCR-RFLP method. We found that there was mostly male than female in NPC
patients in the study with comparison 2.7:1. The age group 41-60 years old
was higher than any other age group with 18 (54.5%) patients along with
Bataknese ethnic with 18 (54.5%) patients. The most common
histopathological type was non-keratinizing squamous cell carcinoma, with 19
(57.6%) patients. There were 23 (69.7%) patients with homozygous wild type
genotype, 2 (6.1%) patients with heterozygous genotype, and 8 (24.2%)
patients with homozygous-mutant genotype. There were patients with
mutant genotype CYP2E1 polymorphism in NPC patients in this study. It can
be the basic data for future research in analyzing the association of this to NPC
and beneficial of CYP2E1 inhibitor in NPC treatment and prevention.
Keywords: CYP2E1 enzyme, nasopharyngeal carcinoma, polymorphism.
116
Histopathology of Lung and Trachea of Mice (Mus

Musculus) Exposed by Formaldehyde Emissions from
Particleboards of Tea Leaf waste (Camellia sinensis
L.)
Iwan Risnasari1*, Arif Nuryawan1 and Difa Prahadita Damanik1
1Faculty of Forestry Universitas Sumatera Utara, Jl. Tridharma Ujung No. 1 Medan
*Corresponding author: iwanrisnasari@gmail.com
Abstract. Production of wood composites such as particleboards still uses

formaldehyde-based adhesives, such as urea formaldehyde (UF) adhesives. UF
adhesives are widely used even though they are known to be harmful to
humans and carcinogenic due to the emissions they produce. Therefore
efforts are needed to produce wood composite products that are relatively
safe to use. One of them is by utilizing tea plant waste which has been known
to have phenolic compounds that can bind to formaldehyde. In this study, the
effect of formaldehyde emission released by particleboard which was made
from tea leaf waste and meranti wood with UF adhesives was carried out in
vivo in mice. After four weeks of exposure in the cage, the mice were then
evaluated for their mucous organs and their blood. From the results of blood
tests and observation of histopathological preparations of mice, it was
concluded that mice exposed to formaldehyde emissions from particleboards
containing tea waste had fewer respiratory organ damage.
Keywords : waste tea leaf, particleboard, formaldehyde emission, mus

musculus, histopathology
117
Assessment of Potential Probiotic Lactic Acid Bacteria

from Tempe and Tape
Sulistiani1*, I Novarina2, Inawati2, A Dinoto1, H Julistiono1, R Handayani1 and S
Saputra1
1Research Center for Biology, Indonesian Institute of Sciences, Cibinong Science

Center, Cibinong-Bogor 16911, Indonesia
2Sekolah Tinggi Teknologi Industri dan Farmasi - STTIF Bogor
*Corresponding author: sulis_lipi@yahoo.com
Abstract. Probiotics are living organisms with many beneficial effects on the
health of host if consumed in sufficient quantities. The many benefits of
probiotics for health, therefore efforts have been directed towards exploring
strains lactic acid bacteria (LAB) as probiotic from fermented food such tempe
and tape. The aim of this study was to assess probiotic candidate from LAB
strains isolated from tempe and tape based on in vitro analysis. A total of 30
LAB isolates were tested for probiotic properties including tolerance to bile
salt and acid, antimicrobial activity, simulated gastric juice (SGJ) and simulated
intestinal juice (SIJ), physiology and enzymatic properties. The results show 8
isolates (Pediococcus pentosaceus Su-ls13, P. pentosaceus Su-ls14,
Enterococcus faecalis Su-ls15, P. pentosaceus Su-ls16, P. pentosaceus Su-ls21,
P. pentosaceus Su-ls22, P. pentosaceus Su-ls24 and Lactobacillus plantarum
Su-ls29) fulfilled the criteria as probiotic candidates, including were able to
produce antimicrobial activity (inhibiting 12 pathogenic bacteria), survived
when exposured in low pH, bile salt, simulated gastric juice and simulated
intestinal juice. The physiology and enzymatic properties demonstrate that all
isolates were able to grow at NaCl 3-6.5%, temperature 30-45C and produce
phytase while six isolates (Su-ls13, Su-ls14, Su-ls15, Su-ls16, Su-ls21, Su-ls22)
were able to produce protease and two isolates (Su-ls22, Su-ls24) were able
to produce amylase.
Keywords: Lactic Acid Bacteria, Probiotic, Tempe, Tape.
118
Improving Cheese-making Process with the

combination of Transglutaminase and Papain
Enzyme
Faustine Sheryl Adeboi1, Nuraini Utari1, Siti Fadhilah Muthmainah1, and Setiadi1*
1Department of Chemical Engineering, Bioprocess Engineering Study Program,

Universitas Indonesia, Kampus Baru UI, Depok, West Java, Indonesia
*Corresponding author: setiadi@che.ui.ac.id
Abstract. Commercially, the cheese product could be manufactured using

rennet enzyme as coagulating agent. It is wisely considered to find an other
option using this kind of enzyme in order to diminish the dependency of
industrial process on the specific limited material. This research is aiming to
develop a cheesemaking process from fresh cow’s milk using the combined
papain and transglutaminase enzymes for increasing the yield and achieving a
better textured cheese product. The milk contains two major proteins, casein
and whey protein, which is important protein to produce the cheese product.
The use of papain enzyme could play an important role as an alternative
enzyme to coagulate the casein milk to produce a cheese. The milk component
of whey proten could be treated with the role of transglutaminase enzyme to
increase the yield and improving the texture through the cross-linking ability.
This cheese-making process with the various addition of papain enzyme to the
the milk and then the yield and texture of cheese were studied. The papain
enzymes used were varied of 0.5; 1.5; and 2.5 grams, and the
transglutaminase enzymes were 0.5; 1; and 2 UE/gram milk protein. The yield
produced, pH, microstructure, texture profile and proximate analysis is
measured. The result showed that the yield of cheese was obtained attaining
13.22%. The proximate analysis showed that addition of TGase increased the
fat and carbohydrate content, lowering moisture and protein content. Texture
profile showed that the cheese product with TGase is harder and springier.
Keywords : Casein coagulation; Cheese; Papain enzyme; Texture;

Transglutaminase enzyme
119
Transformation and Co-expression of pPAMC125 and

pBBRE194 prot-CM1 in Several Strains of Escherichia
coli
Vallery Athalia Priyanka1, Is Helianti2*, Maria Ulfah2, Lina Mulyawati2, and
Abinawanto1
1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas

Indonesia, Depok 16424, Indonesia
2Centre of Bioindustrial Technology, Agency for Assessment and Application of
Technology (BPPT), Laboratoriumof Bioindustrial Technology, LAPTIAB BPPT

Puspitek–Serpong, Tangerang 15314, Indonesia
Priyanka VA and Helianti I contributed equally in this work
*Corresponding author: is.helianti@bppt.go.id
Abstract. In molecular biological techniques, bacterial transformation is an essential

process. Transformation rate into wild type bacteria commonly low because of the cell
defence mechanism of the bacteria. Restriction modification (RM) in bacteria cells can
prevent the introduction of recombinant plasmids into target bacteria. Previously, the
transformation of recombinant shuttle vector pBBRE194 containing protease gene from
Bacillus halodurans CM1 (pBBRE194 prot-CM1) has been conducted. However, the
transformation rate seemed low, and the stable recombinant clones could not be
obtained. In this study, a plasmid with artificial modification (pPAMC125) harbouring
genes encoding for the modification enzymes (methylases) from B. halodurans C125,
and pBBRE194 prot-CM1 were transformed and expressed simultaneously into E. coli
DH5α and Top10.The expression of modification enzymes genes in pPAMC125 in the
presence of different L-arabinose concentration was observed via SDS-PAGE analyses.
The analyse of effect of these genes expression towards plasmid pBBRE194 prot-CM1
were performed by cutting the plasmid with KpnI and PstI, and/or digesting the plasmid
with the extract cell of B. halodurans CM1. The digestion pattern were analysed via DNA
electrophoresis. Certain strain of E. coli would exhibit the optimal condition for RM
genes expression with the result that pBBRE194 prot-CM1 plasmid would not be
completely degraded by CM1 restriction endonuclease.
Keywords : Bacillus halodurans CM1; plasmid artificial modification; restriction

modification; transformation
120
Development of Bamboo Propagation as Source of

Bamboo-based Activated Carbon
Riksfardini A. Ermawar1*, Rikno Harmoko2, N. Sri Hartati2, A. Dani Sunandar3, Ratih
Damayanti3, Saptadi Darmawan3
1Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI

2Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI)
3Forest Product Research and Development Center, Ministry of Environment and
Forestry (KLHK)
1,2Jl. Raya Bogor Km 46, Cibinong - Bogor, 16911
3Jl. Gunung Batu No. 5 - Bogor 16610
*Corresponding author: riksfardini.ermawar@biomaterial.lipi.go.id
Abstract. Bamboo species are widespread in many regions in Indonesia. They

are becoming an important part of Indonesian cultural and social life. They
have been vastly-used and have economic value as renewable resources. They
can be made into various bio-products, including bamboo-based activated
carbon. Here we report characterisation of the activated carbon from six
different bamboo species. Better utilisation of improved bamboos is possible
by generating bamboo non-conventionally, such as tissue culture. Here we
also report, the initiation of an ex vitro and an in vitro propagation of three
chosen bamboo species, namely Dendrocalamus asper, Oxythenantera
abbissinca and Gigantochloa robusta. In vitro propagation was initiated by two
different sterilisation methods. Successful sterile explants would be continue
growing in an induction medium for callus initiation. Young shoots explant
would be cultured on various combinations of auxins including 2,4-
dichlorophenoxyacetic acid (2,4-D), 1-naphthaleneacetic acid (NAA), indole-3-
butyric acid (IBA) and cytokinins (kinetin).
Keywords: Activated-carbon, callus, explant, ex vitro, in vitro, tissue culture
121
Phenotypic and Molecular Changes in Cassava

(Manihot esculenta Crantz.) Genotypes as a
Response to Water-Deficit Stress
Yuni Wahyuni1*, Supatmi1, N. Sri. Hartati1, and Enny Sudarmonowati1
1Research Center for Biotechnology, Indonesian Institute of Sciences,

Jalan Raya Bogor Km.46, Cibinong Science Center, Cibinong-Bogor 16911, Indonesia
*Corresponding author: wahyu004@gmail.com
Abstract. Three local Indonesian cassava (Manihot esculenta Crantz)

genotypes, Adira 4, Roti, and Ubi Kuning retained their growth under water
deficit environment. To understand the physiological and molecular responses
of cassava plants under such stress, we observed leaf stomata characteristics,
i.e. stomatal dimension (width and length) and density, and molecular
expression of aquaporin (AQP) gene, respectively. The observation was
performed on all genotypes on day 27 and 47 after drought treatment.
Quantification of gene expression was performed by comparing the threshold
cycle (Ct) values of AQP with Ct values β-tubulin for each genotype. Results
showed that water deficit treatment significantly affected both stomata
density and dimensions in all genotypes. On day 27 after drought treatment,
the density of stomata in Roti and Ubi Kuning increased two times higher than
the stomata density in plants under water supply. Stomata length of Roti and
Adira 4 under water stress was 0.4 times shorter than those under normal
water supply. Relative expression of AQP in Roti genotype showed that the
gene transcripts increased 0.3 times higher after experiencing drought stress,
indicating induction of gene transcription regulation in the genotype. In Adira
4, the expression of AQP slightly decreased on day 27 after drought stress
compared with those in normal water supply, but on day 47, the stress of the
gene expression was the same as the control. These results showed that three
cassava genotypes responded differently under water deficit stress and these
may serve as basic knowledge for further analysis.
Keywords : aquaporin, cassava, drought stress, stomata characteristics
122
Isolation of an Erythromycin Resistant Gene from

Bacillus halodurans CM1
Widamayanti1, Haniyya2, Is Helianti2*, and Abinawanto1
1Department of Biology, FMIPA Universitas Indonesia,

Kampus UI Depok, Depok 16424, Indonesia
2Center for Bioindustrial Technology, Agency for Assessment and Application of
Technology (Badan Pengkajian dan Penerapan Teknologi),

Serpong, Tangerang Selatan, 15314, Indonesia
Widamayanti and Helianti, I. contributed equally to this work
*corresponding author: is.helianti@bppt.go.id
Abstract. Bacillus halodurans CM1 is one of the bacterial strains from BPPT
Culture Collection which had been isolated from sediments in the Cimanggu
hot spring, West Java, previously. The whole genomic DNA of this strain has
not yet been deeply studied despite its potential as host to produce several
kinds of useful enzymes. Potential genes in B. halodurans CM1 could be
engineered later for further purposes such as antibiotic-resistant genes.
Earlier experiments showed that CM1 strain has the ability to survive in the
media with erythromycin, suggesting there might be genes responsible for this
erythromycin resistance. This study aimed to isolate Erythromycin resistant
gene from B. halodurans CM1 using PCR approach. The primers used for PCR
were designed based on the GenBank database of whole genome Bacillus
halodurans C125. The PCR product was then ligated into pGEM-T easy cloning
vector, and the recombinant vector then transformed into Escherichia coli
DH5α. The DNA fragment with the size of 864 bp was obtained and it encodes
288 deduced amino acids. DNA sequence analysis showed that the gene had
99% similarity with gene encoding erythromycin resistance from B. halodurans
C-125 (GenBank No access: BA000004.3, ErmK). There are 3 nucleotides in the
ErmK sequence of B. halodurans CM1 that are different from that of another
well-known strain B. halodurans C-125. This dissimilar pattern of sequence
encodes Leu instead of Ser in 155th orders of CM1 amino acid sequence.
Keywords : Bacillus halodurans CM1, PCR, cloning, erythromycin resistant

gene
123
Effect of Glucose, Sucrose, and Lactose Solution on

The Stability of Betacyanin Pigment from Red Dragon
Fruit (Hylocereus polyrhizus) Peels
Mukmin Sapto Pamungkas, Edia Rahayuningsih*, and Yuni Kusumastuti
Department of Chemical Engineering, Universitas Gadjah Mada

Jl. Grafika No. 2, Yogyakarta, 55281, Indonesia
*Corresponding author: edia_rahayu@ugm.ac.id
Abstract. People use peels, stems, leaves, and fruits of plants as natural
colorant. One part of fruit peel that has potential used as food colorant is red
dragon fruit peels. The pigments found is betacyanin, but betacyanin
compounds is easily degraded by heat, pH, and light. Degradation of
betacyanin forms betalamic acid compounds (yellow) and cyclo-DOPA acids.
Based on previous research, the betacyanin pigment is stable at pH 5-6. The
addition of ascorbic acid can increase the stability during storage time. In this
work, an attempt was made to increase the stability of betacyanin pigment
against temperature by dissolving betacyanin extract from dragon fruit peels
at 100 ppm and 150 ppm of glucose, sucrose, and lactose solution. Heating is
carried out in a water bath with a heating temperature of 60 oC. The content
of betacyanin was examined by UV-VIS spectrophotometer at a wavelength of
534.8 nm. The results showed that betacyanin was degraded more slowly in
lactose solution. The highest half-life time increased 24.24% for dissolution in
150 ppm lactose. In addition, storaging in lactose solution at room
temperature can inhibit the degradation of betacyanin into its derivative.
Thus, lactose has the potential to increase the stability of betacyanin.
Keywords : betacyanin; food colorant; lactose; stability;
124
Optimization of laccase production from a newly

isolated Trametes sp. EDN134
Fitria Ningsih1, Dede Heri Yuli Yanto2,*, Wibowo Mangunwardoyo3, Sita Heris Anita2,
Takashi Watanabe4 and Asep Hidayat5
1Magister Program in Biology, Department of Biology, Faculty of Mathematics and

Natural Sciences, Universitas of Indonesia, Depok 16424, Indonesia
2Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI),
Jl. Raya Bogor Km. 46, Cibinong Science Center, Cibinong, Bogor 16911, Indonesia
3Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas of
Indonesia, Depok 16424, Indonesia

4Research Institute for Sustainable Humanosphere, Kyoto University Gokasho, Uji,
Kyoto 611-0011, Japan

5Research Center and Development, Research, Development, and Innovation
Agency, Ministry of Environmental and Forestry, Bogor 16610, Indonesia
*Corresponding authors: nfitria960@yahoo.com, dede@biomaterial.lipi.go.id,

wibowo.mangun@ui.ac.id
Abstract. The laccase enzyme is important for the decolorization of textile

dyes. This research aims were to study the optimization of laccase production
from Trametes sp. EDN134 under several variation of substrates (baggase,
bamboo, Jabon wood, oil palm empty fruit bunch (OPEFB), and sorghum
fibers), time incubation (1–15 d), growth temperature (28, 30, 32, 35 and 37
oC), and concentrations of inducer CuSO (1, 2, 3, 4 and 5 mM). The results
4
showed that optimum laccase specific activity (2242 Umg-1) detected in the
OPEFB medium at 10 day’s incubation, temperature 30oC, and CuSO4 2 mM.
The study suggest that these parameters could be used for the optimization
of laccase production from a newly isolated Trametes sp. EDN134. Purification
and characterization will be carried out.
Keywords: Laccase, OPEFB, Trametes sp. EDN134
125
Insecticidal Activity of Clove (Syzigium aromaticum)

and Cinnamon (Cinnamon verum) Essential Oils
against Stored Product Pest, Tribolium sp.
Titik Kartika1*, Nur Ainun2, Anugerah Fajar1, S Khoirul Himmi1, Maya Ismayati1, Ikhsan
Guswenrivo1 and Nova Hariani2
1Research Center for Biomaterials, Indonesian Institute of Sciences,

Cibinong Science Center, Cibinong-Bogor 16911, Indonesia
2Faculty of Mathematic and Natural Science, Mulawarman University
*Corresponding author: titikkartika@biomaterial.lipi.go.id
Abstract. Tribolium sp., a stored product pest is a major pest of stored

products which infest mostly seeds, kernels, and other products. Economic
losses consist of reduced weight and product quality. Two essential oils, clove
and cinnamon, were evaluated for their prospect as insecticide against a
stored product pest, Tribolium sp. The insecticidal activities of essential oils
were determined by contact toxicity, repellency bioassay and F1 progeny
activity which exposed in variant doses. Insect responses, such as mortality
rate, repellence or avoidance behaviour and number of progeny were
evaluated. The bioassays indicated that cinnamon essential oil had superior
activity than clove essential oil, particularly on contact toxicity. The increasing
doses implied on the increasing responses on mortality, repellence responses
and inhibition rate of emerged progeny number.
Keywords : Stored product pest, Tribolium sp., clove essential oil, cinnamon
essential oil, insecticidal activity
126
Antioxidant activity of liverworts Marchantia

paleacea Bertol.
Etti Sartina Siregar1*, Nursahara Pasaribu1 , Muhammad Zaidun Sofyan1
1 Department of Biology, Faculty of Mathematics and Natural Sciences,Universitas

Sumatera Utara, Indonesia. Jl. Bioteknologi No.1 Kampus USU Padang Bulan Medan,
Indonesia, Telp.061-8223564
*email: etti1@usu.ac.id
Abstract. Research on antioxidant activity of Marchantia in Indonesia is still

rarely done. The purpose of this study is to determine the antioxidant activity
of Marchantia paleacea. The method of extraction in this study using
maceration with methanol. The antioxidant activity of extracts were evaluated
by 1,1diphenyl-2-picrylhydrazyl (DPPH). Vitamin C was used as positive
control. The results showed that the extract of M. paleacea has antioxidant
content with LC50 value of 25,25802 μg/mL. The antioxidant activity of M.
paleaceae is classified as a very strong category.The results obtained in the
recent study indicate that M. paleacea is a potential source of natural
antioxidant.
Keywords : antioxidant, DPPH, Marchantia
127
Preparation of Carbon Monolith Based Phenolic

Resin and Its Application for Antibiotic Adsorption
Farhah Ayu Fajrin, Teguh Ariyanto, and Imam Prasetyo*
1Department of Chemical Engineering, Universitas Gadjah Mada

Jl Grafika No 2, Yogyakarta 55281, Indonesia
*Corresponding author: imampras@ugm.ac.id
Abstract.
The presence of antibiotics in wastewater discharged to waterbody has
negative effects. The antibiotics can induce bacteria to be persistent. Hence,
efforts to limit the concentration of antibiotics in wastewater are required. In
this work, removal of antibiotics was performed by adsorption using
nanoporous carbon in the form of monolith. The porous carbon was prepared
by pyrolysis of templated resorcinol formaldehyde polymer at 600°C. The
material was characterized systematically by XRD, SEM-EDX and N2-sorption
analyzer. As comparison, commercial carbon of coconut shell-derived carbon
was also employed in the study. The characterization showed that porous
carbon monolith has specific surface area of 594 m2/g. In the adsorption test,
the results indicated that porous carbon monolith were better at adsorbing
antibiotics compared to the commercial one.
Keywords: adsorption; antibiotics; porous carbon; wastewater
128
EARTH SCIENCE
129
Vertically Propagating of Kelvin waves Near

Tropopause Observed by the Equatorial Atmospheric
Radar (EAR)
Eddy Hermawan1*, Aristyo Rahadian Wijaya2, and Widya Ningrum3
1Center for Atmospheric Science and Technology, National Institute of Aeronautics

and Space Republic of Indonesia (LAPAN), Indonesia
2Department of Meteorology, Faculty of Earth Sciences and Technology, Bandung
Institute of Technology (ITB), Jalan Ganesha 10, Bandung, Indonesia

3Indonesian Institute of Sciences (LIPI), Kampus LIPI Bandung, Jalan Cisitu, Sangkuriang
Bandung, Jawa Barat 40135, Indonesia
*Corresponding author: eddy.hemawan1962@gmail.com
Abstract. The relationship among local convection, vertically propagating

Kelvin waves, and tropopause height variability is examined. This study utilizes
the Equatorial Atmospheric Radar (EAR) observational datasets and NCEP-
NCAR re-analysis data. Spectral analyses both Fast Fourier Transform (FFT) and
Wavelet (WL) techniques are used to investigate the vertically propagating of
Kelvin waves over Kototabang, West Sumatera, Indonesia. In addition, the
statistical analyses both Cross Correlation Function (CCF) and Cross Spectral
(CS) are also done to investigate the correlation between Kelvin waves and
Tropical Tropopause Layer (TTL). Based on the EAR data for the period of
December 2001 to December 2008, the Kelvin waves are detected by
propagation in the zonal wind anomaly eastward and vertically. The analysis
of power spectrum during that time is showing a Kelvin wave which has the
period of 18 days at 17.41 km altitude. Kelvin waves were found near the
tropopause layer (~ 17 km). While, the analysis of power spectrum during wet
and dry season is showing Kelvin wave energy in wet season (DJF=December-
January-February) is stronger relatively than that in dry season (JJA=June-July-
August). In the wet season (DJF) Kelvin wave height of 16.97 MSL (Mean Sea
Level) km is more dominant relatively in the period of 15 days. While, in dry
season (JJA) Kelvin wave is more dominant relatively at 16.52 km altitude with
the period of 18 days. From statistical analysis, the cross correlation between
zonal wind and tropopause height was shown. This means that the zonal wind
direction indicating easterlies affect the Kelvin wave height of the tropopause.
Furthermore, we analyzed Kelvin wave activities during other wet season
(January to March, 2013), transition season (April to June, 2013), and dry
130
season (July to September, 2013), respectively. We found the strongest Kelvin

waves activity was occurred during wet season comparing with other periods
with the period of about 20 days.
Keywords : Kelvin Waves, Propagation, TTL, EAR, and Spectral Analysis
131
WRF Sensitivity to Simulate Early Morning

Precipitation in the Diurnal Cycle over Coastal Region
Erma Yulihastin1,2* Irineu Rakhmah Fauziah3 and Tri Wahyu Hadi1
*Corresponding author: erma.yulihastin@gmail.com
Abstract. Early morning precipitation over coastal region is an uncommon

pattern in the diurnal cycle due to land-see breeze regular mechanism. This
phase shift of diurnal cycle could affect to make a numerical weather
prediction more difficult to capture precipitation. This study aims to
investigate sensitivity of WRF model on simulating early morning precipitation
over north coast of West Java. Some cases (26 January 2006, 4, 8 February
2008) represent heavy rainfall have simulated in capturing early morning
precipitation over north coast of West Java to examine model sensitivity on
convection scheme and domain size. The result showed that BMJ scheme
could simulate early morning precipitation quite well on phase, whereas KF
scheme capture out of phase on diurnal cycle. For model sensitivity on domain
size, the larger domain more realistic in phase to simulate early morning
precipitation. For synoptic condition during heavy rainfall, it is appeared that
Cross Equatorial Northerly Surge which is supported by South China Sea-Cold
Tongue play main role to develop early morning precipitation.
Keywords : WRF, sensitivity, early morning precipitation, coastal region
132
Application of Time-Series Sentinel-1A for Land

Deformation in Central Aceh, Indonesia
Jefriza1, Izham Mohamad Yusoff1, Ismail Ahmad Abir2, Saumi Syahreza3,6,*, Pakhrul
Razi4 , Habibah Lateh1, Muhammad Rusdi5
1School of Distance Education, Universiti Sains Malaysia, Malaysia

2School of Physics, Universiti Sains Malaysia, Malaysia
3Physics Department, Faculty of Mathematics and Natural Sciences, Universitas Syiah
Kuala, Indonesia
4Physics Department, Faculty of Mathematics and Natural Sciences, Universitas
Negeri Padang, Indonesia

5Remote Sensing and Cartography Lab, Universitas Syiah Kuala, 23111 Darussalam,
Aceh, Indonesia
6Tsunami and Disaster Mitigation Research Centre (TDMRC), Unsyiah, Banda Aceh,
23233, Indonesia
*Corresponding author: ssyahreza@unsyiah.ac.id
Abstract. Various decorrellation phenomena of Interferometric Synthetic

Aperture Radar (InSAR) have led researchers to develop several Multi-
Temporal (MT-SAR) techniques with the application of time series/stack of
images. InSAR involves the interaction of electromagnetic waves known as
interference, to measure precise distances between the target and the sensor
to derive a landscape topography and its subtle changes in elevation. Central
Aceh, located in North of Sumatra Island, Indonesia was chosen for the study
area as the Island consists of soft soil and Sumatra Faults from the geological
point of view. Data were acquired from Sentinel-1A and ALOS PALSAR-1
satellites, where Quasi-Persistent Scatterer (Q-PS) and Small Baseline Subset
(SBAS) methods were applied respectively. From the results, the subsidence
and uplift areas were mapped and verified with others publication. The
velocity subsidence at Nunang and Musara Alun villages were found to range
from 2.4 to 5.7 and 0.6 to 2.3 mm/year, respectively which corresponded to
the results obtained by other research publication. The deformation in Central
Aceh needs continuous monitoring using InSAR as the place is a landslide
prone area because of the nature of the terrain connected to the earthquake
zones.
Keywords : Sentinel-1A, ALOS-PALSAR, Q-PS, SBAS, subsidence and uplift.
133
Flood Inundation Mapping Using Synthetic Aperture

Radar Data Dual Polarization: A Case Study of Flood
in Lake Tempe, South Sulawesi – Indonesia
Nur Febrianti1*, Fajar Yulianto1, Muhammad Arda1 and Nanik Suryo Haryani1
1 Remote Sensing Application Center, LAPAN, Indonesia
*Corresponding author: nur.febrianti@lapan.ac.id, nfebrianti@gmail.com
Abstract. Remote sensing data can be used to help disaster management and
environmental management because this data has advantages in terms of
speed, is more efficient, can reach large and remote areas, has consistency in
measurement, can make repeated measurements, and has measurable
accuracy. Floods in Lake Tempe occur almost every year due to overflowing of
Lake Tempe. This research will detect flood inundation from Sentinel-1 data with
dual-polarization of flooding in Lake Tempe, South Sulawesi. The data used are
Sentinel-1 data before (2 May 2018) and after (26 May 2018) flood events. Both
of these data are used to identify changes from inundation that occur. The
combination of RGB composite Pre_VH, Post_VH, Post_VV shows a very wide
flood inundation distribution. The results of the model validation show an
accuracy value of greater than 75%.
Keywords : Composite, Dual-polarization, Sentinel-1, Pre flood, Post flood
134
Land Surface Temperature Estimation Before the

Aceh Tsunami (Case Study of West Aceh)
Muhammad Rusdi
Remote Sensing and Cartography Lab, Universitas Syiah Kuala, 23111 Darussalam,
Aceh, Indonesia
*Corresponding author: emrusdi@unsyiah.ac.id
Abstract. Thermal channels that mounted in Landsat satellite imagery can be

used to estimate land surface temperature. Moreover, using its temporal
resolution, the estimation of temperature at the past could be achieved. Such
as, temperature estimation before tsunami. Surface land estimation require
Land Surface Emissivity (LSE) value which could be achieved with NDVI
methods (Normalized Difference Vegetation Index). Result shows land surface
temperature in Aceh Barat District in 2013 was 26.07 °C. The result also shows
that the spatial distribution has a spread pattern based on its transect.
Temperature in the coastal areas has higher temparature than in the high land
areas. This phenomenon due to landuse optimalization in by the settlement in
the coastal areas, while vegetation dominated in the highland areas.
135
Calibration Of Rainfall Ensemble Prediction Of

ECMWF System 4 Using Bayesian Model Averaging
Robi Muharsyah1,2*, Tri Wahyu Hadi2, Sapto Wahyu Indratno3
1Atmospheric Sciences Research Group, Faculty of Earth Sciences and Technology,

Institute Technology Bandung, Bandung, 40132, Indonesia
2Agency for Meteorology, Climatology and Geophysics of Republic of Indonesia
(BMKG), Jakarta, 107720, Indonesia

3Mathematics, Faculty of Matemathics and Natural Sciences, Institute Technology
Bandung, Bandung, 40132, Indonesia
*Corresponding author: robi.muharsyah@gmail.com
Abstract. Bayesian model averaging (BMA) is a statistical post-processing

method for producing probabilistic forecasts from ensembles in the form of
predictive PDFs. It is known that BMA is able to improve the reliability of
probabilistic forecast of short range and medium range rainfall forecast. This
study aims to develop the application of BMA to calibrate seasonal forecast
(long range) in order to improved quality of seasonal forecast in Indonesia. The
seasonal forecast used is monthly rainfall from the output of the ensemble
prediction system European Center for Medium-Range Weather Forecasts
(ECMWF) system 4 model (ECS4) and it is calibrated against observational data
at 26 stations of BMKG in Java Island in 1981 – 2018. BMA predictive PDFs is
generated with Gamma distribution approach which is obtained based on
sequential training windows (JTS) and conditionals training windows (JTC).
BMA-JTS approach is done by selecting the width of the 30-month training
window as the optimal training period while the BMA-JTC is carried out with a
cross-validation scheme for each month. In general, Both of BMA-JTS and
BMA-JTC better than RAW models. BMA-JTC calibration results are varying
according to spatial and temporal, but in general the result is better in the dry
season and during the El Nino phase. BMA is able to improve the distribution
characteristics of the RAW model ECS4 prediction which is shown by: a smaller
value of Continuous Rank Probability Score (CRPS), a larger value of the
Continuous Rank Probability Skill Score (CRPSS) and more flat form of the
Verification Rank Histogram (VRH) than the RAW model. BMA also increases
the skill, esolution and reliability of prediction of probability Below Normal
(BN) and Above Normal (AN), which is known from the increasing Brier Skill
136
Score (BSS), and the increasing area under curve of Relative Operating
Characteristics (ROC) compared to the RAW model. Furthermore, the
reliability of BN and AN of BMA results also has the category of “still very
useful” and “perfect” compared to RAW models that are in the “dangerous”,
“not useful” and “marginally useful” categories. The reliability of BMA results
with the category “still very useful” and “perfect” show that the probabilistic
forecast of BN and AN events can be used in making decisions related to
seasonal forecast.
Keywords : Bayesian Model Averaging, Sesonal forecast, ECMWF system 4,

Model reliability
137
Community-based
development and
socioeconomic
science
138
Socio-economic Analysis of Traditional Diamond

Mining Community in Banjarbaru City, South
Kalimantan Province
Nisrina Akmiyati1, Rizki Munawwarah Daimoko1, Restu Ulfah2, Hilal Najmi1, Ali Yansyah
Abdurrahim3*, Maila Dinia Husni Rahiem4, Munasprianto Ramli4
State Islamic Senior High School (MAN) Insan Cendekia Tanah Laut, Indonesia
1
2State Islamic Junior High School (MTsN) 8 Banjar, Indonesia
3Research Center for Population, Indonesian Institute of Sciences (LIPI), Indonesia
4Faculty of Tarbiyah and Teachers Training, Syarif Hidayatullah State Islamic
University, Indonesia
*Corresponding author: aliyansyah.lipi@gmail, aliy002@lipi.go.id
Abstract. Since the 8th century, the South Kalimantan region was known as a
diamond producer, especially in the Cempaka Sub-District, Banjarbaru City.
Local communities have carried out the work as a traditional diamond miner
for generations in groups with a system of division of labor and results. This
study aims to determine the effect of diamond mining on socio-economic
conditions the miners, especially income and expenditure. Primary data
collection was carried out in Mei-August 2018 in Cempaka diamond mining
location through field observations, in-depth interviews, FGDs, and
questionnaires. Based on David Ricardo's theory, the informants and
respondents were grouped into three categories: field miners (pendulang),
landowners, and diamond polisher. The analysis shows that diamond mining
contributes to all, but with different income levels. The average monthly
income of pendulang (Rp 1,147,222.22) is the smallest compared to diamond
polisher (Rp 2,125,000.00) and landowners (Rp 3,625,000.00). For average
expenditures per month, each of which is sequentially Rp 1,186,239.35; IDR
1,020,658.33; and Rp 1,273,950.00. This indicates a deficit between income
and expenditure of pendulang. The deficit was covered by various programs
from government, such as food assistance for poor households (‘Raskin').
Besides, the study shows differences in socio-economic characteristics of
three mining categories.
Keywords: Community, livelihood, mining, social-ecological system, socio-

economic.
139
Dilemmas of New Rich People: Social Changes in

Rural Communities Affected by Kediri Airport
Development
Najwa Kahani Fatima1, Nur Farida1, Ali Yansyah Abdurrahim2*, Maila Dinia Husni
Rahiem3, Munasprianto Ramli3
1State Islamic Junior High School (MTsN) 2 Kediri City, Indonesia

Abstract. The new airport development project by a private company in Kediri

was one of the biggest factors that caused social change in rural communities
in four villages (Bulusari, Tarokan, Grogol, Jatirejo) in Tarokan, Kediri Regency.
Land acquisition was held at a high price, then the people suddenly got
abundant money. Their unpreparedness to manage compensation money
caused dilemmas in managing their money. This research was conducted to
analyse social changes, especially in their lifestyle and mindset. Problems are
analysed based on Gillin and Gillin’s social change theory, Minor and Mowen’s
lifestyle theory, Dweck’s mindset theory, and Peter Berger’s social class
theory. This study uses a mixed-method by conducting field observations,
qualitative interviews, FGDs, and quantitative questionnaires in 2019. The
results of the study showed that there were social changes in lifestyle,
mindset, and social class. Although they experienced a dilemma in using their
money, such as for consumptive purposes that could improve their lifestyles,
some of them finally decided to improve their livelihood sustainability, such
as buying new land (79%), new business (29%), & saving (86%). In addition,
based on a religious mindset, most of them spend their money on matters of
worship: hajj only (14%) and umrah+hajj (43%).
Keywords: Land use change, lifestyle theory, mindset theory, sosial change,
rural community.
140
Rural Livelihood Strategy in Sumatra After ‘the

ASEAN 2020 Haze Free Policy’ Implementation
Aulia Afifatuz Zulfah1, Rafiska Chelsie Howitzerni1, Afryansyah1, Ali Yansyah
Abdurrahim2*, Munasprianto Ramli3, Maila Dinia Husni Rahiem3,
1State Islamic Senior High School (MAN) Insan Cendekia Ogan Komering Ilir, Indonesia
Abstract. A series of regulations to realize ‘the ASEAN Haze Free Policy in 2020’
have been made, starting from the international level to the local level,
including the ban on the use of fire for traditional agricultural land clearing
(sonor) that was commonly used by rural communities in Sumatra. This study
analyzes the livelihood strategies of rural communities after the issuance of
the policy. The data used are primary and secondary. Primary data were
collected through interviews, FGDs, and field observations in June-August
2019 at the district level (Ogan Komering Ilir District) and villages (Lebung
Gajah Village, Tulung Selapan Sub-District). On the other hand, the secondary
data were conducted during January-September 2019. Analysis conducted
using the Scoones' Sustainable Livelihood Approach found that to ensure the
sustainability of their livelihoods, various households in the rural community
carried out strategies: (1) agriculture intensification (‘Kebun Pangan Mandiri
Program’), (2) livelihood diversification (raising livestock, catching fish,
building swallow houses, & becoming construction worker), and (3) activating
women's groups (household financial and business management). In
implementing their strategies, they exchange their access to livelihood assets
that are owned and available through social institutions and formal
organizations in the village.
Keywords: agricultural strategy, disaster, haze, rural community, sustainable

livelihood.
141
Effectiveness Of Promotion About Nutrition With A

Lecture Method And Leaflet Media On Knowledge
And Attitude Of The Mothers Who Have A Child
Under Five Years
Arlinda Sari Wahyuni
Faculty of Medicine, Universitas Sumatra Utara

Jl. Dr. Mansyur no 5 Medan
*Corresponding author: arlinda@usu.ac.id
Abstract.Children under five years old as the nations next generation who are
expected to become quality resources in the future need special attention on
the nutritional status of children. In the Wintarti study, 2015 conducted by
Suiraoka, et al. 2010 that related to education or nutrition counseling with
leaflet media on family who are conscious on nutrition (KADARZI), it was found
that there were differences between the results of pretest and post test given
to intervention groups with smaller statistical test media 0, 05. This study aims
to analyze the effectiveness of counseling with lecture and lecture methods +
leaflet media on changes in knowledge and attitudes of mothers who have
children under five about the nutrition of children under five in Sipahutar
District Health Center, North Tapanuli in 2019. The research method used
Quasi Experiment with a two group pre-test and post test approach with 86
sample with lecture groups and 86 sample with lecture groups + leaflets. The
sampling method used the determination of the sample formula to test
hypotheses the data of the proportions of two populations according to
Lemeshow formula. The results of this study indicated that the knowledge and
attitudes of mothers about the nutrition of children underfive years at pretest
and posttest are different significantly between maternal knowledge and
attitudes before counseling with after counseling , statistically the value of p =
<0.001, which means that there is a difference in changes in knowledge and
attitudes of respondents between groups who given counseling with the
lecture and lecture groups and leaflets.
Keywords : Attitude, Extension. Knowledge, Leaflet, Media, Lecture
142
Partnership In Management Of Private Forest In

Megamendung Village, Bogor District
Messalina. L. Salampessy1, Indra G Febryano2, Iim Rostani3
1,3Faculty
of Forestry, University of Nusa Bangsa, Bogor, Indonesia
2Departement of Forestry, Faculty of Agriculture, University of Lampung,
Bandar Lampung, Indonesia
*Corresponding author: meisforester76@gmail.com
Abstract. Partnership in community forest management is one of the efforts

to improve the welfare of community forest farmers. Forest management with
the community has been identified as having great potential to improve the
sustainability of the forests function. The purpose of this study is to analyze
the institutional performance of partnerships between farmers and forest
landowners. The study was conducted in May - July 2019 in the village of
Megamendung, Bogor Regency. Data collection was carried out using
questionnaires, interviews and field observation involving 30 forest farmers
who partnered in this management. The collected data is analyzed and
presented descriptively. The results showed that the performance of
community forest management partnerships in community forests was quite
diverse which was influenced by social relations between owners and farmers,
compliance with agreement agreements, supervision and sanctions for
violating agreements, as well as the proportion of input-output sharing from
management activities undertaken. There are several findings that need to be
considered in conducting a partnership program, namely the readiness of
farmers participating in the partnership and increasing the capacity of farmers
resources in developing this partnership.
Keywords : Institution, partnership, community forest management, forest

farmers
143
APPLIED SCIENCE
AND TECHNOLOGY
144
Removal of Fe(II) from aqueous solutions by chitosan

activated carbon composite study isoterm and
kinetic adsorption
Putri Wulan1*, Agus Prasetya1 and Yuni Kusumastuti1
1Departement of Chemical Engineering, Faculty of Engineering, Gadjah Mada

University, Jogyakarta
*Corresponding author: putriwulan31284@gmail.com
Abstract. The high levels of the Fe(II) metal can be reduced by using an
adsorbent in the adsorption process. The adsorbent used is a chitosan
activated carbon composite. This research aimed to examine the effects
chitosan activated carbon composite in adsorption equilibrium time and
kinetics model of metal ion Fe (II) adsorption. Composites were prepared by
adding 1,5 grams of activated carbon into 1.5 % (w/v) chitosan solution then
dropped into a 2.8 % NaOH solution mixture. The adsorption process was
carried out in a batch system with 0.5; 1; dan 1,5 gram of adsorbent weight at
operation temperature 25oC, 35oC and 45oC containing 50 mL metal ion Fe(II)
24.8 mg/L with time variations of 5, 10, 20, 40 and 60 minutes. This research
showed that the chitosan activated carbon composite effectively remove
metal ion Fe (II) in aqueous solution with the equilibrium reached in 60
minutes maximum 84.42 % metal ion Fe(II), with 1.5 gr of adsorbent weigth
at operation temperature 45oC, fitted well the Langmuir isotherm model and
the kinetic model followed pseudo-second order model.
145
Pyrolysis Of Palm Oil Coconut Biomass With Pig Iron

Catalyst
Yufita Tuhuteru1,Muslikhin Hidayat1, Hary Sulistio1
1Department of Chemical Engineering, Faculty Of Engineering,Universitas Gadjah

Mada 55281 Yogyakarta, Indonesia
*Corresponding author: mhidayat@ugm.ac.id, jufitatuhuteru95@gmail.com
Abstract. Biomass pyrolysis is a thermochemical decomposition process that

produces coke, tar, and gas products. This study aims to determine the optimal
temperature changes to the results of the reduction of iron ore (Fe) produced,
as well as gases formed from pyrolysis of biomass with a temperature variation
of 450,500,550,600,700,8000C. In this study, dehydration was previously
carried out aimed at eliminating water content in iron ore after the pyrolysis
process was carried out using palm oil (CKS) with iron ore. Iron ore is analyzed
qualitatively by color appearance analysis and also analysis by instrumentation
namely by XRD and XRF and gas products are analyzed by GC. The results
obtained from iron ore are the best quality in the 700-8000C process
conditions. Iron ore has a blackish color display that indicates magnetite
properties. Results of XRD magnetite analysis of 42.5% and XRF showed the
composition of iron ore at a reaction temperature of 700-8000C at 60.74% and
60.51%, the gas yield was obtained from the time difference taken from 0.5 to
0.3% CO, CO2 0.8-0.4%, CH4 0.6-0.2%, H2 0.4% from 7000C.
Keywords : pyrolysis, tar decomposition, reduction, iron ore
146
Characterization and Reduction Behavior of Carbon-

Infiltrated Goethite Ore by Impregnation of Tar
Recovered from Coke Oven Gas
Asful Hariyadi1, Rochim Bakti Cahyono1* and Suryo Purwono1
1Department of Chemical Engineering, Universitas Gadjah Mada

Jl. Grafika No. 2, Yogyakarta 55281, Indonesia
*Corresponding author: rochimbakti@ugm.ac.id
Abstract. The Iron/Steel industry is currently facing serious problem due to

excessively dependent on metallurgical coke, which is produced a large
amount of CO2 emission and it was limited source in Indonesia. Coke-free iron-
making is one of the efficient method to reduce the amount of cokes. Carbon-
infiltrated iron ores were prepared by impregnation process from coaltar
solution and goethite ore at 500 oC and 600 oC to promote direct reduction in
iron making. These variables were systematically characterized by XRD and
SEM/EDX. Pore distribution and BET surface area were determined by N 2
adsorption-desorption measurement. The XRD signals attributed to Fe2O3 in
the goethite ore were completely disappeared and replaced by signals
attributed to Fe3O4 after impregnation process. Cross-sectional analyses of
composite particles prepared using both temperatures showed that tar-
derived carbonaceous materials have completely either impregnated or vapor
infiltrated into the mesoporous in pyrolyzed goethite ore. Carbon-infiltrated
goethite ore showed a higher reduction degree than conventional coke. It is
confirmed that high reactivity of carbon-infiltrated iron ores is due to close
contact between the goethite ore and carbon in its nanoporous interior
facilitated the fast reduction.
Keywords : carbon, coal tar, goethite, ironmaking, impregnation
147
Properties of polyninyl alcohol composite filled

Ampel bamboo (Bambusa vulgaris) microfibrils
fibrillated by mechanical treatment
Kurnia Wiji Prasetiyo1*, Fakhruzy2 and Wida Banar Kusumaningrum1

2Faculty of Forestry University of Muhammadiyah Sumatera Barat Padang, Indonesia
*Corresponding author: jundiazzam@yahoo.com
Abstract. Composite for food packaging are mostly made from petroleum-
based synthetic polymers that have long been degraded naturally. This
condition encourages the study of the use of cellulose microfibrils or organic
materials as fillers and reinforcement for food packaging composites to reduce
the dominance of synthetic polymers. The purpose of this study was to
determine the characteristics of polyvinyl alcohol composites with fibrillated
cellulose microfibrils of Ampel bamboo pulp (Bambusa vulgaris) whether or
not bleached. Pulp from Ampel bamboo produced by the soda process was
fibrillated using a disc refiner in 10, 20 and 30 times repetition variations.
Microfibril concentration of 0.1 wt% dry based on the weight of polyvinyl
alcohol (PVA) matrix. Composite preparation was carried out by dissolving
polyvinyl alcohol using a hot plate stirrer at 80 °C, 300 rpm for 30 minutes. The
composite solution is poured in a mold measuring 20 x 16 x 0.1 cm.
Morphology characteristics from microfibrils was evaluated by scanning
electron microscopy (SEM). The mechanical characteristics tested with UTM
Shimadzu 1 kN in accordance to ASTM D 882-75b standard. PVA composites
filled with cellulose microfibrils from bleached Ampel bamboo pulp have
better mechanical characteristics than composites with unbleached pulp.
Mechanical treatment in various replications in the disc refiner machine can
increase tensile strength, tensile modulus and composite elongation.
Keywords : cellulose microfibrils, composite, fibrillation, polyvinyl

alcohol, pulp of Ampel bamboo
148
Selective Voltammetric Detection of Dopamine Using

Ferrocene Modified Carbon Paste Electrode
B A Widyaningrum1, Novia Widianti2, Muji Harsini2, Aning Purwaningsih2

2Department of Chemistry, Universitas Airlangga,
Kampus C Mulyorejo, Surabaya , East Java 60111, Indonesia
*Corresponding author: muji-h@fst.unair.ac
Abstract. A selective sensor based on carbon paste electrode with ferrocene

(Fc/CPE) has been investigated. The Fc/CPE was prepared for selective
dopamine (DA) in presence of interference compounds as ascorbic acid (AA)
and uric acid (UA). The Fc/CPE can well resolve the peak oxidation of DA, AA
and UA. The modified electrode exhibited better electrocatalytic activity and
catalytic efficiency 3.5 times higher than using the unmodified electrode. The
electrochemical behaviors of DA at the surface of the modified electrode were
studied by cyclic voltammetry (CV). Using differential sweep voltammetry
(DPV) at optimum condition the determination of DA were measured in the
concentration range of 0.5 μM – 10 μM with a detection limit of 0.2437 μM
and a correlation coefficient of 0.9995. The Fc/CPE has been applied to
determination of DA spike into simulated sample and showed good recovery.
Keywords : selective; carbon paste electrode; ferrocene,; cyclic voltammetry;

differential pulse voltammetry
149
The Effectivity of Concentrated Maleic Acid for

Nanocellulose Isolation from Betung Bamboo
(Dendrocalamus asper sp) Pulp
Wida B. Kusumaningrum1*, Putri Amanda1, N. Masruchin1, and Lisman Suryanegara1

*Corresponding author: wida.banar@biomaterial.lipi.go.id
Abstract. Modificatin and synthesis of nanocellulose have attracted growing

interest in the last decade due to applicable for many fields on advanced
materials. Nanocellulose which perform as cellulose nanocrystals (CNC) and
cellulose nanofibrils (CNF) possess great properties, such us high surface area,
high tensile strength and stiffness depend on crystal sructure, and accessable
hydroxyl groups. Organic acid hydrolysis could be as a newest eco-technology
for nanocellulose isolation. Some advantages could be performed such us
recycable solid acid, high thermal stability, accesable carboxyl groups that easy
to synthesis for various product, one step processing which could produce CNC
and CNF. The objective of this research is to study the effectivity of maleic acid
anhydrous for nanocellulose isolation. Unbleached and Bleached Pulp of
Betung Bamboo were used as cellulose resources. Concentrated maleic acid
anhydride in 50% up to 60% (w/w) for 45 up to 90 minutes at 100 oC of reaction
temperature were conducted to isolate cellulose nanocrsytals from betung
bamboo pulp. Higher yield of CNF was obtain than CNC from acid hydrolysis
process. FTIR analysis show that carboxylic groups was detected at wavelength
1730 cm-1 for all samples. High thermal stability also resulted for CNF by
organic acid hydrolysis.
Keywords : cellulose nanocrystal, cellulose nanofibers, maleic acid, acid

hydrolysis, betung bamboo
150
Kinetics of soursop leaves antioxidant extraction

through microwave assisted extraction technique
Megawati1*, Astrilia Damayanti1, Karnowo2, Rizqy R. Ginting3, Gagah M. Bungsu1, and
Muhammad H. F. Rizky1
1Chemical Engineering Department, Faculty of Engineering, Universitas Negeri

Semarang, Indonesia
2Mechanical Engineering Department, Faculty of Engineering, Universitas Negeri
Semarang, Indonesia
3Department of Chemical Engineering, Graduate School of Engineering, Hiroshima
University, Japan
*Corresponding author: megawati@mail.unnes.ac.id
Abstract. The purpose of this research is to study the kinetics of soursop leaves
antioxidant extraction through microwave assisted extraction technique. The
antioxidant extraction was conducted with ethanol as the solvent (1:13 v/v)
using microwave assisted extraction at various powers (300, 450, and 600 W)
for 2-10 min. The extraction kinetics is approached that the rate limiting step
is only the antioxidant mass transfer from the particle surface to the bulk of
solution. In addition, the mass transfer of antioxidant is expressed by second-
order rate law. The extraction results showed that microwave power and
extraction time strongly influenced the extraction yield. The highest total
phenolic concentrations of soursop leaves extract antioxidant obtained at 600
W and 8 minutes was about 0.608 mg/mL. From the kinetics calculation, the
extraction speed and extraction capacity constants increase in proportion to
the increase in power.
Keywords : antioxidant, kinetics, microwave assisted extraction, soursop leaf.
151
The Performances of bark flour as filler in Phenol

Formaldehyde in Glulam Manufacturing from
Benuang and Duabanga Wood
Sari Delviana Marbun
IPB University, Jalan Raya Dramaga No. 53, Dramaga, Bogor, 16680
*Corresponding author: sari.delvianamarbun@gmail.com
Abstract. Enviromental issue has triggered many efforts to utilize renewable

biomass for replacing non-renewable resources. In this research, bark tree
fillers were used to reduce the utilization of petroleum-based adhesive. The
aim of this research was to analyze the effect of benuang (Octomeles
sumatrana/ BN) and duabanga (Duabanga moluccana/ DB) barks as a fillers of
phenol formaldehyde (PF) resin for the BN and DB glulam manufactures. Four
gluing mixtures were used, with ratio PF: technical filler: bark filler 10:0:0;
10:1.5:0.5; 10:1.25:0.75; and 10:1:1. Characteristics of bark fillers and
adhesive mixtures were investigated. Moisture content, density, cold and
boiling water delamination, bonding strength, wood failure, and
formaldehyde emission of glulam were determined according to JAS 1152
standard. Results showed that high oil absorption of BN and DB bark fillers
influenced the increasing of adhesive viscosity. GCMS analysis showed that
BN and DB bark filler have different phenolic compounds and concentration,
which may affect the different reactivity of these bark fillers with
formaldehyde. DSC analysis showed that adhesive mixtures cured at a lower
temperature than the control (PF). Analysis of variance on glulam properties
showed that the addition of 2 part bark filler to the phenol formaldehyde resin
system did not decrease glulam performance. The high wood failure of BN and
DB glulam in the bonding test proved that bark fillers improved the bonding
strength.
Keywords: bark filler, benuang, bonding strength, duabanga, glulam
152
Influence of Transglutaminase Enzyme

Incorporated into Protein based Edible Coating for
Preservation of Spanish Mackerel Fish
(Scomberomorus commersoni)
Sauria Karina1*, Setiadi2, Siti Fadhila3 & Nuraini Utami4
1,2,3&4
Bioprocess Engineering Study Program, Department of Chemical Engineering,
Faculty of Engineering, Universitas Indonesia, Depok 16424, West Java, Indonesia
*Corresponding author: karinasauria27@gmail.com
Abstract. Transglutaminases have been utilized in food technology to modify

protein to improve rheological properties of food material or product. Edible
coating is viscous solution that contains polymer structures, forming thin
layer that covers surface of food material. Edible coating maintains quality of
food product by becoming a barrier, and protecting the surface from
spoilage from internal and external causes. In this study, several
concentration of transglutaminase enzyme were applied, acting as cross-
linking agent into protein based coating solution for coverage of Spanish
mackerel fish meat. The fish meat samples were stored inside fridge with
temperature of 10oC. Quality of fish meat was assessed each 5 days during 15
days of storage. The assessment of quality includes water content, pH and
total volatile base of nitrogen (TVBN). Fourier Transform Infra-Red (FT-IR)
was used to identify the activity of transglutaminase enzyme by the changes
of detected functional group. Addition of transglutaminase has improved
barrier properties of edible coating that maintained water content of fish
meat better than control sample, but the by-product of enzymatic reaction
has decreased quality of fish meat according to pH and TVBN results. FTIR
spectroscopy showed changes in the secondary structure of protein (amide
II).
Keywords : Transglutaminase, Cross-linking, Edible coating, Spanish

Mackerel Fish, Spoilage
153
Development of Mobile-Based Application for

Practical Wood Identification
Muhammad Rosyid Arifin1,2, Esa Prakasa1, Bambang Sugiarto1, Riyo Wardoyo1, Yan
Rianto3
1Research Center for Informatics, Indonesian Institute of Sciences (LIPI), Bandung,

Indonesia
2Department of Informatics, Faculty of Engineering Universitas Sangga Buana YPKP,
Bandung, Indonesia
3Center for The Utilization and Innovation of Science and Technology, Indonesian
Institute of Sciences (LIPI), Cibinong, Indonesia
*Corresponding author: muhammadrosyidarifin@gmail.com
Abstract. Wood identification is important task especially for wood anatomist.

The identification process is required in many fields, such as custom ports,
forest survey, and wood industries. However, expertise on wood identification
is limited to few wood anatomists. To enable identification work can be
applied in broader area, a mobile based application is proposed to conduct
wood identification. The paper describes the development steps of mobile
based application for wood identification systems. A dataset of Indonesian
commercial wood surface images was collected from Xylarium Bogoriense,
Indonesian Ministry of Environment and Forestry. The images were collected
by using smartphone camera at optical magnification of 180x. The collected
images were then used to find the weights of deep learning architecture. The
trained model is then stored in cloud-based server at Research Center for
Informatics, Indonesian Institute of Sciences. A mobile application, namely
AIKO stands of Indonesian words - Alat Identifikasi Kayu Otomatis (Automatic
Tool for Wood Identification), is developed to enable image acquisition and
images transferring to the server. AIKO application sends the acquired image
to the trained identification model in the server. The output of identification
model is the species name of the observed images. Besides species/botanical
name, AIKO also provides information on trade names, specific
gravity/density, durability class, strength class, commercial timber
classification, and recommended utilization.
Keywords: wood identification, deep learning architecture, mobile based
application
154
Performance Test Of Adsorption Of Nickel Ions With

Porous Carbon From Mlanding Wood Which Has
Been Oxidized With Hydrogen Peroxide
Chici Wardiani Prasongko1, a *, Teguh Ariyanto2,b and Chandra Wahyu Purnomo3,c
1Departement of Chemical Engineering, Universitas Gadjah Mada, 55281 Yogyakarta

Indonesia
2Advanced Material and Sustainable Mineral Processing Research Group, Universitas
Gadjah Mada, 55281Yogyakarta, Indonesia

3Agrotechnology Innovation Center, PIAT UGM, Berbah Sleman, Yogyakarta, Indonesia
*Corresponding author: chici.wardiani.p@mail.ugm.ac.id,

bteguh.ariyanto@mail.ugm.ac.id, cpurnomo@ugm.ac.id
Abstract. This research was conducted to study changes in functional groups

from porous carbon synthesized from mlanding or petai sawdust after being
oxidized with H2O2 at variations in temperatures of 30°C, 60°C, and 80°C. In
addition, this research was also carried out to study the effect of porous
carbon after oxidation of nickel ion adsorption. Adsorption experiments were
carried out using a solution of Hexammine Nickel (II) Nitrate [Ni(NH3)6](NO3)2
synthesized by adding Nickel (II) Nitrat Heksahidrat (>99,9%, Merck) to
Ammonia solution (25wt%, Merck). The nickel solution was shaken for 48
hours using a waterbath shaker at a temperature variation of 30°C, 40°C, and
60°C. The results of this study indicate that the oxidation of porous carbon will
increase functional groups in porous carbon such as lactone, carboxyl, and
phenol. For the adsorption of nickel ions by porous carbon from mlanding
wood follows the Langmuir isotherm plot and shows better performance for
absorbing nickel ions in solution compared to carbon that is not oxidized
Keywords : Mlanding Wood, Porous Carbon, Oxidation, Adsorption, Nickel Ion.
155
Modification of Rice Husk for Eco friendly

Particleboard
Prabu S. Sejati1*, Sukma S. Kusumah1, Wahyu Dwianto1, Dadang Surjasa2

2Faculty of Industrial Technology, Trisakti University
Jalan Kyai Tapa No. 1 Grogol, Jakarta Barat, Indonesia
*Corresponding author: pssejati@gmail.com
Abstract. Rice husk and rice straw are some of abundantly available
agricultural waste that could substitute the utilization of wood as
particleboard materials. Nevertheless recent studies show that rice husk and
rice straw provoke low mechanical and physical properties of particleboard.
Modification of rice husk and rice straw particle was investigated in order to
improve particleboard properties by immerging rice husk and straw in boiling
water (100°C) for 0.5, 1, 2 hour and in Na2CO3 (80°) for 1 hour. The boards
were manufactured under pressing conditions of 200°C for 10 min used citric
acid and sucrose as adhesive. Based on the results of this study, the density
and moisture content of all the particleboards were satisfied the requirement
of the JIS 5908 (2003). Rice husk pre-treatment by immerging in water and
Na2CO3 resulted improvement in physical and mechanical properties of
particleboard than the untreated rice husk particle. Modification of rice husk
by 1 hour water boil shows the best performance of all parameters.
Keywords : Rice husk, Rice straw, Modification, Particleboard, Pre-treatment
156
Resistance Characteristics of Natural Rubber

Compounds to Dimethyl Ether (DME) and DME / LPG
Mixtures
Hani Handayani1*, Adi Cifriadi1, Arief Ramadhan1, Asron F. Falaah1, Nanang
Hermawan2 and Emi Yuliarita2
1Research Center for Rubber Technology, Indonesian Rubber Research Institute, Bogor
16151, West Java, Indonesia

2Pusat Penelitian dan Pengembangan Teknologi Minyak dan Gas Bumi, Lembaga
Minyak dan Gas Bumi (LEMIGAS), South Jakarta 12230, Indonesia
*Corresponding author: hani.ppkbogor@gmail.com
Abstract. The use of Liquified Petroleum Gas (LPG) in Indonesia is quite high,
the volumes reaches 6.7 million tons per year. Indonesia currently still imports
70% of its domestic consumption of LPG, or about IDR 40 trillion. Imports of
Liquid Petroleum Gas (LPG) continues to increase every year. Dimethyl ether
(DME) is one alternative of renewable fuels because its availability in the
country is quite abundant. When DME applied as household fuels, some
components such as metals and rubber should resistance to DME, such as
stoves, rubber hoses, rubber components in regulators and tube valves.
Lemigas has succeeded in developing a DME resistant stove but still not found
a rubber that is resistant to DME. This research aim to find a natural rubber
formula that is resistant to DME and DME / LPG mixture. The study was
conducted by varying the dose of sulfur (0.5, 1.5, and 3.5 phr) using 2 types of
elastomer namely natural rubber and NBR rubber. Immersion was carried out
in DME and 3 variations of the DME/LPG mixture (10/90, 20/80, 30/70). The
results showed that the compound formula using natural rubber with a sulfur
dose of 1.5 phr has the best resistance to DME and DME/LPG mixtures.
Keywords : dimethyl ether, DME, LPG, natural rubber, resistance
157
Characteristics of alkaline treated binderless

particleboard made from sorghum stalks
L Astari1, WB Kusumaningrum1, A Zamilla2

2Faculty of forestry, Kuningan university, Indonesia
*Corresponding author: astari@biomaterial.lipi.go.id
Abstract.Since formaldehyde-based resin argued to deteriorate human

health, binderless particleboard turns as alternative to the usage of those
conventional resin. Binderless board has superior mechanical properties yet
the dimensional stability is low. The objective of this study was to investigate
the effect of alkali treatment to the sorghum stalks fibres as well as properties
of the board. Sorghum stalks was soaked in 5odium hydroxide (NaOH) for 30
minutes then rinsed with tap water and dried until the moisture content of
particles was not less than 5The treated particles were used to produce
binderless particleboard. The pressing condition was 180°C temperature for
10 minutes and 2.5 MPa pressure. Characterization of fibres after treatment
was confirmed using FTIR and light microscope. Properties of particleboard
was investigated to its physical and mechanical properties. The result shows
that the superior mechanical properties of particleboard given by
particleboard with pre-treated particle.
Keyword : Alkaline treatment, binderless, Sorghum stalk
158
Enhancement of Color, Dimensional Stability, and

Durability of Fast-growing Teakwood by Mild
Pyrolysis
Aprilia Kartikawati1, Imam Wahyudi1*, Gustan Pari2, Lina Karlinasari1
1Forest Products Department, Faculty of Forestry, IPB University, Bogor, Indonesia

2Forest Research Development and Innovation Agency (FORDA), Bogor, Indonesia
*Corresponding author: imyudarw16@yahoo.com
Abstract. Effect of mild pyrolysis on changes of color, dimensional stability,

and durability of fast-growing teakwood has been investigated. Wood samples
were extracted from the 5- and 8 year-old of teak trees from man-made forest
areas of Jasinga and Ciampea, Bogor District, West Java Province, Indonesia,
respectively. All parameters were measured in green condition. The color
CIELab* system was used for wood color measurements, while determination
of dimensional stability was analyzed by coefficient of anisotropic (T/R-ratio).
The non-choice feeding test to Coptotermes curvignathus Holmgren in
laboratory scale was used to evaluate wood durability. Mild pyrolysis was
conducted with a combination of 170, 200, 230 °C for 4 and 6 h within a
reactor. Result showed that total color change (ΔE) increased along with the
increase of temperature and time. Treated wood surface became darker and
difference between heartwood and sapwood color were reduced significantly.
Compared to untreated wood, dimensional stability and durability of treated
woods well improved. Their T/R-ratio was smaller, while their mass losses
caused by termite attack were lower. Considering the changes in wood color,
dimensional stability, and durability, therefore, the best recommended
treatment was mild pyrolysis at 200 °C for 4 hours.
Keywords : dimensional stability, durability, fast-growing teakwood, mild

pyrolysis, wood color
159
Antimicrobial Activity of Toona sinensis dried

extracts from Toba and Borobudur Regions,
Indonesia
Maya Ismayati1*, Deni Zulfiana1, Dita Meisyara1, Apriwi Zulfitri1, Didi Tarmadi1, Khoirul
Himmi Setiawan1, Anugerah Fajar1, Titik Kartika1 and Sulaeman Yusuf1.

*Corresponding author: maya_ismayati@biomaterial.lipi.go.id

Abstract. This research evaluated the antimicrobial activity of
some extracts from Toba and Borobudur regions. Ten plant
extracts were applied into antimicrobial bioassay against Escheria
coli, Bacillus subtilis, Salmonela typii, Staphylococcus aureus,
Candida albicans, and Candida tropicalis. The antimicrobial activity
of treatment extracts were determined by calculating the
diameter of the treatment inhibition zone. Three replicates was
carried out with water as a control. Among the plant extracts, T5,
Toona sinensis was presented as the most active in inhibiting the
growth of C. albicans, B. subtilis, S. typii and E. coli with the
diameter growth about 2.00, 1.80, 1.33 and 1.33 cm, respectively.
Based on those results, T. sinensis was continued for fractionation.
n-hexane, ethyl acetate and methanol extract was isolated from
crude extract of T. sinensis. Three fractions also were evaluated for
antimicrobial bioassay. At 1% concentration, all fractions have
shown activity in inhibiting the growth of all microbes. However,
specifically each fraction shows activity against certain microbes.
The n-hexane fraction shows the greatest inhibitory activity for E.
coli and S. typii; ethyl acetate fraction for B. subtilis and C. albicans;
and methanol fraction for E. coli and S. typii. The results of present
study showed that the T. sinensis plant extract present potential
antimicrobial. Further investigation needed to observe the mode
of action onto microbial growth that caused by chemical content
of S. sinensis.
160
The influences of social-community behavior into

ants-pest distribution
Ikhsan Guswenrivo1*, Dita Meisyara1, Widia Nur Khasanah2 and Nova Hariani2

2Faculty of Mathematics and Natural Sciences, Mulawarman University
Samarinda, East Kalimantan, Indonesia
*Corresponding author: Ikhsan.guswenrivo@biomaterial.lipi.go.id
Abstract. Household ants are an important group of insect pests

in the urban environment, but the difference of social status in the
society will have different perspective of ants as a household pest.
Questionnaire-based surveys of homeowners from differences
location and human living environments reveal that half of
respondents felt that ants were economically important. While the
other half though that the existence of ants did not gave any effect
into their daily life. Eighteen species of ants were found indoors
and outside buildings; 5 species from Formicinae, 7 species from
Myrmicinae, 3 species from Ponerinae, and 3 species from
dolichoderinae. The respondents were not aware that ants can
medically vector pathogenic organisms and most of respondents
used aerosol sprays to control as well as physically technic.
Keywords : household ants, pest status, Indonesia
161
The Health Safety of Three Types of Kratom

(Mitragyna speciose Korth.) Powder based on Fungal
Flora Analyses
Nilam Fadmaulidha Wulandari1*, Elly Kristiati Agustin2 and Mad Aidi3
1Research Center for Biology, Indonesian Institute of Sciences,

2Research Center for Plant Conservation and Botanical Garden,
Indonesian Institute of Sciences, Bogor 16122, Indonesia

3PT. Ampera Java Gloval. Jl. Ampera GG. Ampera Baru, No. 1,
Pontianak, Kalimantan Barat, Indonesia
*Corresponding author: microbesnilam@gmail.com
Abstract. The fungal flora was analyses for ascertaining the safety
standards of three types of the kratom powder. The laboratory
work showed interesting result. Not only on the fungal artificial
media, such as malt extract agar, but also in bacterial medium such
as EMBA, PLA and XLD. The three kratom types (green kratom, red
kratom1 and red kratom2) showed the low score of fungal number
as NA-DFC (The National Agency of Drug and Food Control)
requirement as less than 5 x 107 colony/g. The important result
must be considered were the possibility of mycotoxin
contamination produced by fungi harbouring the kratom. Further
studies were needed to evaluate the mycotoxin contamination in
the three types of kratom powder. The description of the fungi as
additional data will be outlined detailed in this paper.
Keywords : Aspergillus spp.; food safety; Mitragyna speciose;
phenotypic characters
162
Termite Biodiversity in an Urban Conservation Area,

Cibinong Science Center–Botanical Garden, Bogor,
Indonesia
Khoirul Himmi1*, Astuti Latif2, Didi Tarmadi1, Ikhsan Guswenrivo1, Anugerah Fajar1,
Maya Ismayati1, Titik Kartika1, Sulaeman Yusuf1, and Nova Hariani2
1Research Center for Biomaterials, Indonesian Institute of Sciences (LIPI),

2Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman
University
*Corresponding author: khoirul_himmi@biomaterial.lipi.go.id
Abstract. Termite distribution in tropical region is highly

influenced by temperature, landscape changes, and
anthropogenic activities. The present study evaluated termite
biodiversity and distribution in an urban conservation area,
Cibinong Science Center-Botanical Garden (CSC-BG), located in
Bogor, West Java, Indonesia. CSC-BG area is 189 hectare (ha),
which covers various landscape: arboretum (ecological park),
botanic plantations, and research center buildings. Termite survey
had been conducted on teak wood plantations of 5, 6 and 9 years
old. A 2 m x 100 m belt-transect was employed at those 3
plantations. Transect area was divided into 20 sub-transects (2 m
x 5 m), in which one sub-transect was surveyed for 30 minutes by
2 persons. In total, 89 termite specimens were collected. The
results suggested that the recovered termites were dominated by
termitidae family, consisting of fungus-growing termites from
genus Macrotermes, Microtermes, Odontotermes; and soil
feeding termites from genus Pericapritermes. The present of soil
feeding termites is important, as they are sensitive to the
anthropogenic disturbances and landscape changes. The older the
plantation, the higher the number of recovered specimens and the
species richness.
Keywords : Termite biodiversity, urban conservation area,
Cibinong Science Center-Botanical Garden
163
Efficacy of Several Entomopathogenic Microorganism

As Microbial Insecticide Against Insect Pest on Chili
(Capsicum annum L)
Apriwi Zulfitri*, Ni Putu Ratna Ayu Krishanti, Anis Sri Lestari, Dita Meisyara and Deni
Zulfiana
Research Center for Biomaterials, Indonesian Institute of Sciences,

*Corresponding author: apriwi.zulfitri@lipi.go.id
Abstract. The purpose of this study was to apply microbial

suspension as bio-insecticide based on potential
entomopathogenic microorganisms and to observe its
effectiveness in controlling insect pest on chili plants, Capsicum
annum L. The bioinsecticides consisted of suspension of
entomopathogenic fungal and bacterial which were successfully
isolated in previous studies. The experiment was carried out in chili
cultivation area in Situ Gede, Bogor. The application used spray
method with multiple various doses. The observation showed that
the treatments of entomopathogenic fungal spore suspension of
Metarhizium sp. T4B23, B2-2, and entomopathogenic bacterial cell
suspension of Bt + SP4, were able to resist the attack of insect
pests such as whitefly, grasshopper and armyworm. In addition,
the treated plant had more vigorous growth and yield than the
control treatment.
Keywords : bioinsecticide, chili plant, entomopathogen, field
experiment
164
Selected of Potted Anthurium Clones through

Hybridization
Ridho Kurniati 1) and Eka Fibrianty 1)
1)Indonesian Ornamental Crops Research Institute
Jalan Raya Ciherang- Segunung- Pacet, Cianjur, PO. BOX 8 Sdl Cianjur, 43253
West Java – Indonesia
*Corresponding author : Fildzaku@ yahoo.co.id
Abstract. Assessement of variability potted anthurium based on

consumer preference was important for selection best potted
anthurium. The selected clones were registered as new varieties
candidate. The assessment was conducted on September 2019,
in Indonesian Crops Research Instutite - West Java. The objectives
of the study was to choose best potted anthurium through
conventional breeding based on consumer preference. Twelve
potted anthurium clones were used as materials for assessment.
There were 100 respondences to select the best potted anthurium
for register as new varieties candidate. The respondence were
farmers of ornamental plants, floriculture policy maker, hobbies,
private company in floriculture and ornamental plant decorator.
The potted anthuriums had some variations of color, size and
shape both of spadix and spathe. The highest consumer choice
was purple potted anthurium with purple and white spadix. The
seconds choice was red combined green color of spathe and red
spadix, and the third was orange potted anthurium. All of selected
clones were propagated through in vitro mass propagation and
registered as new varieties potted anthurium.
Keywords:Anthurium, Assessment, Consumer Preference, New

varieties.
165
Poster presentation
166
FOREST
SCIENCE
167
P1-01
Effect of Malic Acid Content and Pressing
Temperature on the Performance Properties of
Hybrid Fiberboard from Mahang Wood (Macaranga
hypoleuca) and Bamboo (Dendrocalamus asper)
Agus wahyudi
Balai Litbang Teknologi Serat Tanaman Hutan

Komplek BP2TSTH Kuok
Jl. Raya Bangkinang - Kuok km 9, Kotak Pos 4/BKN Bangkinang - Riau 28401
*Corresponding author: agus.kuok@gmail.com
Abstract. This study investigated physical and mechanical properties of hybrid

medium density fibreboard made from 90f mahang wood and 10f petung
bamboo ratio with malic acid binder. Malic acid in this research were set at
20253035nd 40ased on dry weight fibers, while the target density was set 0,7
g/cm3. Pressing temperature were set at 160ºC, 180ºC and 200ºC with the
pressing time was 10 minutes. Physical and mechanical properties test were
according to Japanese Industrial Standard A 5905 for fiberboard. The result
showed that addition of malic acid and pressing temperature significantly
increased the quality of hybrid medium density fibreboard from mahang wood
and petung bamboo. Infrared analysis indicated that the present of ester
linkages much higher with the increasing of malic acid content and pressing
temperature. The best properties of hybrid medium density fibreboard could
be achieved at a malic acid content of 25tnd pressing temperature of 200ºC.
168
P1-02
Effect of different extraction methods on starch yield
extracted from oil palm trunk (Elaeis guineensis
Jacq.)
DA Pramasari1*, N Aini2, D T Wibowo1, F A Syamani, D H Y Yanto1 and S S Kusumah1
1Research center for Biomaterials, Indonesia Institute of Sciences (LIPI) Jalan Raya
Bogor Km. 46, Cibinong, Jawa Barat, Indonesia 16911
2Dept. of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University
*Corresponding author: dwi.ajias@gmail.com
Abstract. The significant amount oil palm trunk (OPT) during replanting of the
senescent oil palm trees is available abundantly in Indonesia as side product
of oil palm plantation site. OPT could be utilized as a starch-producing raw
material. Meanwhile, the process of starch extraction method of OPT still need
improvement for increasing starch yield extracted. The previous research, the
effect of mechanical extraction methods of OPT for improving starch yield
extracted has been investigated. Further research was evaluation of chemical
extraction methods by mixing the OPT with sodium metabisulphite (Na2S2O5),
the concentration 0.5% (w/v) at room temperature for their effect on starch
yield. The experimental results indicated that of steeping using sodium
metabisulphite improved the starch yield (6.80%) as compared to that of
mechanical extraction method (0.05%). Furthermore, the extraction method
using by sodium metabisulphite is indicated prospective method for OPT
stacrh-producing raw material than mechanical extraction.
Keywords : extraction; mechanical, metabisulphite, starch
169
P1-03
Physical and Mechanical Properties of Five
Indonesian Wood Treated with Polystyrene
Ismail Budiman1*, Renny Purnawati2), Herman Siruru3), and Yusuf Sudo Hadi4),

Cibinong Science Center, Cibinong-Bogor, Indonesia
2Papua University, Manokwari, Papua Province, Indonesia
3Pattimura University, Ambon, Maluku Province, Indonesia
4Forest Product Department, Faculty of Forestry, IPB University, Bogor, Indonesia
*Corresponding author: budimanismail@gmail.com
Abstract. Five Indonesian wood i.e. sengon (Falcataria moluccana), manii

(Maesopsis eminii), pinus (Pinus merkusii), duabanga (Duabanga mollucana)
and maniani (Flindersia pimenteliana) were treated with polystyrene. The
purpose of this study was to determine the physical and mechanical properties
of treated wood. Air-dried samples were placed under vacuum at 600 mmHg
for 30 minutes, which was followed by immersion in monomer styrene and
pressure at 10 kg/cm2 used varied for 30, 60, and 90 minutes respectively. The
wood samples were then wrapped in aluminium foil and placed in an oven at
103±2 C for 24 hours. Furthermore, the aluminium foil was removed and the
samples were weighed for polymer loading calculation. The test of
impregnated wood conducted by refers to the British Standard BS 373:1957,
comprised moisture content, density, water absorption, shrinkage swelling,
modulus of rupture, modulus of elasticity, and hardness. Results showed that
wood treatment with monostyrene could improve the physical and
mechanical properties of wood.
Keywords: treated wood, impregnated, polystyrene, physical properties,

mechanical properties
170
P1-04
Forest/Land Fire Smoke Detection Using GCOM-C
Data
Khalifah Insan Nur Rahmi, Nur Febrianti, Indah Prasasti
Remote Sensing Applications Centre, LAPAN

Jl. Kalisari No. 8, Pekayon, Pasar Rebo, Jakarta Timur, Indonesia
*Corresponding author: khalifah.insan@lapan.go.id
Abstract. Forest/land fire occur every year in Indonesia especially in peat land
area and increased in dry season. These fires give bad impact to air pollutant
from smoke fire events. Smoke fire need to be identified the distribution
periodically. GCOM-C data is new release data could be used to detect smoke
fire. This study has objective to identify smoke fire from GCOM-C data. GCOM-
C data has wavelength range from 0.38 to 12 µm it covers visible, near
infrared, short-wave infrared and thermal infrared. It is relatively similar to
MODIS or Himawari-8 images which could identify smoke fire also. The
methodology is adapted from MODIS and Himawari-8 smoke identification
based on the wavelength used. Hotspot data is overlaid with GCOM-C image
to know the location of fire events. VN8 bands could identify hotspot of
September 2018 in Central Kalimantan.
Keywords : smoke, land/forest fire, GCOM-C, hotspot
171
P1-05
Diversity of vegetation types in three forest
ecosystems, Mappi, Papua
Laode Alhamd*
Lab. Ecology, Soil and Litters. Research Center for Biology, Indonesian Institute of
Sciences,
*Corresponding author: trialbgr@mail.com
Abstract. Diversity of vegetation types in three type forest ecosystems, Mappi,

Papua. Observations were made using 10 plots, 20 x 20 m2 in size of each plot
(tree, diameter (d) ≥ 20 cm), 10 x 10 m2 (pole, diameter 10 ≤ d <20 cm), 5 x 5
m2 ( sapling , d <10 cm) and 2 x 2 m2 (seedling). All plants in the plot are
inventoried according to growth rate. The location of the observation is based
on land cover, with land cover in the form of shrubs, secondary dry land forest
and primary dry land forest. The results showed that the ecosystem in the
swamp forest is located on the banks of the river with vegetation cover varying
up to the mainland, such as Pandanus tectorius, Stenoclaena palustris, Cerbera
manghas, where S. palustris species is the type that mostly covers the forest
floor. In the secondary forest cover, the Keing type stands are derived from
Tristaniopsis ferruginea, Canarium denticulatum, Rhodamnia cinerea, Aporosa
sp. and Palaquium sp. While primary dryland forests have stands of several
species with high canopy and the forest floor covered by thin litter is lowland
forest ecosystems, such as Santiria apiculata, S. laevigata, Cryptocarya
crassinervia, Fagraea racemosa, Gonystylus macrophyllus, Vatica rassak and
Prunus arborea.
Keywords : Biodiversity, Mappi, growth rates, shrubs, land cover
172
P1-06
Diversity of Medicinal Plants In Yard In Several
Villages in Indonesia
Mulyati Rahayu*, Leberina Kristin Ibo, Septiani Dian Arimukti and Siti Susiarti
Research Center for Biology, Indonesian Institute of Sciences,

*Corresponding author: mulyati_r@yahoo.com
Abstract. In Indonesia, yard or home garden as one of the processed

land has not received full attention. One of the roles of the yard is as
a “living pharmacy”. Research and inventory of plants grown in the
yard of 11 villages in Indonesia recorded no less than 120 plant species
were used as medicinal herbs. Some were also used as traditional
cosmetics and spices. Zingiberaceae family are the most commonly
used and cultivated plants. Information on the results of this
ethnobotany research is expected to provide input to improve yard
development
Keywords : yard, medicinal herbs, ethnobotany, Indonesia.
173
P1-07
Novel Application of Citric Acid Adhesive in The
Manufacturing of Eco-friendly Plywood Made from
Jabon (Anthochephalus cadamba)
Jajang Sutiawan1, Sukma S Kusumah2*, B A Widyaningrum2, D A Pramasari2, Dede
Hermawan1, MY Massijaya1, IM Sulastiningsih3
1Departement of Forest Product, Faculty of Forestry, IPB University,

IPB Dramaga Campus, Bogor 16680, Indonesia

3Forest Products Research Insitute,
Jl. Gunung Batu, Bogor 16610, Indonesia
*Corresponding author: sukma.surya@biomaterial.lipi.go.id
Abstract. The development of natural adhesives derived from non-fossil

resources is very important for the future. The research present effects of
pressing temperature and time on physical properties plywood made from
fast growing tree species, namely jabon (Anthochephalus cadamba). 2 mm tick
jabon veneers was used in the manufacturing plywood. The citric adhesive
with the glue spreads of 137 g/m2 and concentration of 59 wt% applied using
single glue line. Veneers jabon that had been spread with adhesive were dried
at 80 °C for 12 h and then hot pressed under a pressure maximum 5 Mpa and
various temperature and time conditions, i.e., 180–200 °C and 4–10 min,
respectively. The physical and mechanical properties of were evaluated based
on Japanese Agriculture Standard 234 for plywood (JAS 234-2003). The result
showed plywood manufactured under effective pressing conditions had good
physical and mechanical properties and effective pressing temperature and
time were 200 °C and 4 min, respectively. Fourier transform infrared (FT-IR)
spectra analysis suggested that the ester linkages resulting from the reaction
between citric acid adhesive and jabon.
Keywords : citric acid; eco-friendly, jabon; plywood
174
P1-08
Biodiversity study of several peatland types in Papua
S Sundari1*, L K Ibo1, J S Rahajoe1, L Alhamd1, H Gunawan2, N C Priyono2
1Botany Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI),
Jl. Raya Jakarta Bogor KM. 46 Cibinong Science Center, Cibinong 16911
2Peat Restoration Firm (BRG), Jl. Teuku Umar No.10, Menteng, Central Jakarta 10350
*Corresponding author: ssundari1977@gmail.com
Abstract. Research on biodiversity study of several peatland types in Papua

had been carried out in Merauke and Mappi. Plot method was used for
ecology research and interviews method for ethnobotany research. The
results showed that peatland in Kaliki, Merauke was dominated by
Beilschmiedia sp., Carallia brachiate (Lour.) Merr., and Kibara coriacea
(Blume) Hook. f. & A. Thomps, while in Marga Mulia was dominated by
Melaleuca leucadendra (L.) L only. Peatland in Khanami, Mappi was dominated
by Semecarpus forstenii Blume, Archidendron clypearia (Jack) I.C. Nielsen, and
Campnosperma auriculatum (Blume) Hook.f., whereas in Yame was
dominated by Calophyllum euryphyllum Lauterb, Diospyros toposiodes King &
Gamble, and Syzygium effusum (A.Gray) Müll. Stuttg. The use of plants as
building materials for houses and ships, medicinal materials, food ingredients,
animal feed, energy-producing materials/firewood, and material in traditional
rituals.
175
P1-09
Cell Wall Thickness and Occupancy Measurements of
Sumba Island Wood Species
Wahyu Dwianto1*, Danang S. Adi1, Sung-Wook Hwang2, Junji Sugiyama2

Cibinong Science Center, Bogor 16911, Indonesia
2Research Institute for Sustainable Humanosphere, Kyoto University
*Corresponding author: wahyudwianto@yahoo.com
Abstract. Previous results from Sumba Island exploration found 35 potential

wood species, which were classified into strength class II to IV based on their
specific gravity (sg) values. Among various anatomical features, it was
considered that cell wall thickness (CWT) and cell wall occupancy (CWO) were
important parameters to sg values, because cell walls supported the wood
strength. Therefore, this research aimed to know the relationships between
sg with CWT and CWO. Samples with size of 1 cm in length and 0.5 cm in
diameter were taken from the representative wood species which having sg
around 0.30 to 0.80. Wood samples were cut in cross section by a sliding
microtome, with a thickness of 15 µm. The images were captured with a light
microscope equipped with digital camera. Furthermore, CWT and CWO were
calculated by R program based on the average of wall and lumen of all cells in
the one picture area. The results showed that sg increased with the increasing
of CWT and CWO. By linear regressions, sg values seemed more affected by
CWO than CWT, probably because CWO also calculated lumen portions of cell
walls.
Keywords : CWT; CWO; specific gravity; strength class
176
BIOSCIENCE
177
P2-10
Characterization and effectiviness test of Rhizobium
bacteria from various plants on soybean (Glycine
max L) plants in greenhouse
S Purwaningsih, D Agustiyani and S Antonius
1 Microbiology Divison, Research Center for Biology, Indonesian Institute of Sciences

Jl Raya Jakarta-Bogor Km 46 CSC Cibinong, West Java
*Corresponding author: sipur2005@yahoo.co.id
Abstract. Rhizobium bacteria are bacteria that are included in Plant Growth
Promoting Rhizobacteria (PGPR), which is capable of nitrogen fixing,
phosphate solubilizing, producing protease enzymes and siderophore
production, having an important role in increasing agricultural productivity.
Several isolates have been isolated from 8 legumes from various regions, with
the aim of obtaining potential, effective and efficient Rhizobium isolates as
biological fertilizer agents. A total of 8 isolates were successfully isolated using
selective media for Rhizobium bacteria, YEMA, and qualitative activity tests
were carried out, such as N fixing, phosphate solubilizing, siderophore
production, IAA hormone and protease enzyme production. All isolates
flourished in YEMA media, in the YEMA + BTB media 8 isolates fast growing. 8
isolates that have high activity are: EKP (3), EKP (4), i (1), 1 (2), B (1) H (2), A
(2), 2 (1) which are characterized as isolates Rhizobium was tested on soybean
plants using sterile sand media in green house. The design used was a
completely randomized design with 3 replications for each treatment. The
results of the study showed that 8 isolates inoculated against the soybean
plants varied, increasing growth. Isolates number H (2) and 1 (2) (isolates of
Peanut root nodules) gave the highest yield on soybean growth.
Keyword : Rhizobium isolates, soybean, sterile sand medium
178
P2-11
Isolation Indole Acetic Acid (IAA) producing Bacillus
siamensis and optimization of the physicochemical
conditions for IAA production
Suliasih1* and Sri Widawati1

*Corresponding author: lishadari@yahoo.co.id
(All authors contributed equally to this work)
Abstract. Indole acetc acid (IAA) is a phytohormone which one of important

compound for plant growth and development, affecting as inducing cell
elongation, cell enlargement and cell division. IAA is synthesized of both in
plants and microorganisms. For microorganisms, production of phytohormone
IAA is one of the essential criteria for plant growth promotion. In present
study, IAA producing bacterium Bacillus siamensis was isolated from peat soil
in West Kalimantan and optimize some culture condition of IAA production.
The production of IAA were determined in TSB and YEMB liquid medium and
the optimization of IAA production in culture media was carried out at
different of incubation time, L-Tryptophan concentrations, carbon and
nitrogen sources, pH and temperature. The result revealed that maximum IAA
production of Bacillus siamensis was achieved at 96 hours of incubation.
TheYEMB medium supplemented with 250 ppm of tryptophan was obtained
as the best medium for IAA production (9.89 µg/ml). This bacterium produced
optimum levels of IAA in the presence of sucrose (10.23 µg/ml) and thryptone
(16.61 µg/ml) as carbon and nitrogen sources respectively. In the physical
parameters, optimum condition for IAA production were found at T 35oC and
a pH of 8.0 in the amount of 8.42 µg/ml and 13.29 µg/ml respectively
Keywords : Bacillus siamensis, Indole Acetic Acid (IAA)
179
P2-12
Assessment on Lead and Cadmium Bioaccumulators
for Phytoremediation Contaminated Rice Fields in
Bekasi Districts, West Java
Nuril Hidayati* and Dwi Setyo Rini
Research Center for Biology- Indonesian Institute of Sciences

Jl. Raya Jakarta - Bogor Km 46, Bogor 16911
West Java-Indonesia
*Corresponding author: nurilhid@yahoo.com
Abstract. Most of agricultural areas in Bekasi Districts of West Java, which are
well known as rice production centers, are contaminated by heavy metals,
predominantly by lead (Pb) and cadmium (Cd). This research aims to assess
lead (Pb) and cadmium (Cd) contamination in paddy fields in Bekasi Dictricts,
to investigate potential Pb and Cd accumulator plants and possible solution
of using the selected accumulator plants for Pb and Cd phytoremediation in
the contaminated sites. The results revealed that there are six plant species
namely Colocasia sp. that accumulated up to 5,254 ppm Pb and 0.421 ppm Cd,
Ipomoea fistulosa Mart. ex Choisy that accumulated 4,302 ppm Pb and 0,511
ppm Cd, Eichhornia crassipes (Mart.) Solms that accumulated up to 4,685 ppm
Pb and 0,474 ppm Cd, Hymenachne amplexicaulis (Rudge) Nees) that
accumulated 4,378 ppm Pb and 0,478 ppm Cd, Saccharum spontaneum L. that
accumulated 5,181 ppm Pb and 0,478 ppm Cd and Acorus calamus L. that
accumulated 4,884 ppm Pb and 0,411 ppm Cd within 9 weeks. The highest BCF
for root Pb was 0.671 in S. spontaneum and the highest BCF of Pb in I. fistulose
was 0.558. Whereas the highest BCF for Cd at the root was found in S
spontaneum at 0.566 and BCF for Cd at the highest shoot of 0.475 in S
spontaneum. Translocation Factor (TF) for Pb is highest at 0.904 in I. fistulose
and 0.828 in S spontaneum, While TF for Cd is highest at 0.927 in A. calamus
and 0.839 in S. spontaneum.
Keywords : Bioaccumulator, cadmium, contamination, lead, rice field,

phytoremediation
180
P2-13
Isolation and identification of Ascomycetous yeast
producing phytase
Atit Kanti*, Idris and I Made Sudiana
InaCC-Bidang Mikrobiologi, Pusat Penelitian Biologi-LIPI

Jl. Raya Jakarta-Bogor, Km 46, Cibinong-Bogor 16911
*Corresponding author: atityeast@gmail.com
Abstract. The objective of study was to isolate, identify and characterize phytase
producing yeast. The yeast was isolated from various resources, and identification of
yeast was conducted by sequencing of ITS region. Of 41 isolates, 24 isolated produced
phytase. The highest phytse producing yeast were identified as Candida natalensis YEG
132, Saturnispora quitensis YEg 135, and Candida albicans YEg 226. These isolates were
able to use various carbon and nitrogen sources. The maximum phytase production
depend on isolates, media composition, pH and incubation temperature. Temperature
affected phytase production. All isolates produce the highest phytase was at 30°C.
Increased temperature to 35° C and 40°C inhibits phytase production by all strains. S.
quitensis was the highest isolate inhibited by an increase of incubation temperature.The
pH of cultures affect phytase production and varies depend on isolate. S. quitensisand
Candida natalesis produced the highest phytase activities at pH 5.0, while C.
albicanswas at pH 6.0. All isolates were able to assimilate glucose, dextrose, sucrose,
xylose and lactose. In general, glucose was the best carbon sources for phytase activity.
But each isolate has their our preferential on carbon sources. S. quitensis produced the
highest phytase on glucose, but not on dextrose. This isolates produce almost the same
amount of phytase when grown on sucrose, xylose and lactose. C. natalensis also
produces highest phytase on glucose, but the produce almost the same amount of
phytase when grown on dextrose, xylose, lactose and sucrose. C. albicans produced the
highest phytase when they were grown on glucose, dextrose and sucrose. Each
isolates has preferential on nitrogen sources. Yeast extract was best N-sources for C.
albicans, while ammonium sulphate was best for C. natalensis, S. quitensis. Ammonium
nitrate was not favorable for phytase production by C. natalensis.Candida natalensis
YEG 132, Saturnispora quitensis YEg 135, and Candida albicans YEg 226 were good
candidate for phytase production.
Key words: Ascomyceteous yeast, Candida natalensis YEG 132, Candida albicans YEg
226, phytase production.
181
P2-14
Utilization Of Acetonitril And Benzonitril As
Substrates And Identification Of Its Conversion
Products by rhodococcus pyridinovorans strain i-
benzo Isolated From Leather Tanning Waste, Garut
Nunik Sulistinah1 and Bambang Sunarko1
1Research Center for Biology, Indonesian Institute of Sciences, Cibinong Science

Center, Cibinong-Bogor 16911, Indonesia
*Corresponding author: nuniksulistinah@gmail.com
Abstract. Nitrile compounds are produced and extensively used in various

industries. Widely use in industries will cause pollution so that it will have a
negative impact on health. Most of them are known to be very toxic and
carcinogenic. Some microbes are reported to be able to utilize both aliphatic
and aromatic nitrile as substrates and convert them into compounds that have
economic value. Based on these microbial abilities, the application of nitrile
hydratase, amidase and/or nitrilase enzymes synthesized by microbes for the
production of various pharmaseutics, other important compounds increase
sharply. One of the microbes proven to be able to convert nitriles is the
Rhodococcus group. A bacterium capable of utilizing high concentration of
acetonitrile and benzonitrile has been isolated leather tanning waste in Garut
and identified as Rhodococcus pyridinovorans. The bacterium grew on 0.2 - 2.0
M acetonitrile, and 5-25 mM benzonitrile, the best growth was shown at 500
mM acetonitrile and 15 mM benzonitrile. Metabolites of acetonitrile and
benzonitrile were detected . Morphology characteristic of R. pyridinovorans
strain I-benzo is a gram-positive bacterium with orange colony in colour, non
motile and not spore. The main purpose of this work was to determine the
ability of R. pyridinovorans I-benzo strain to grow on nitriles, convert
acetonitrile and benzonitrile to ammonia and carboxylic acid as a final product
Keywords : Rhodococcus pyridinovorans, benzo-I strain, hydrolysis,

acetonitrile, benzonitrile, nitrile converting enzyme, amidase, nitrile hydratase,
nitrilase, ammonia, carboxylic acid, aliphatic and aromatic nitrile
182
P2-15
Effect of Dyeing Repetition and Color Reinforcement
Materials on Cloth Color by Mixed Fungi Dyes
Suciatmih
e-mail: suciatmih2008@yahoo.ca
Abstract. Colors variation and aging on cotton cloth can be obtained through
dyeing repetition and the application of color reinforcement materials. In the
present study, cotton cloth was dyed with dyes from the mixed fungi of
Aspergillus and Paecilomyces using three different dyeing repetitions (3, 6, and
9 x), three different mordants (alum, CaCO3, and FeSO4.7H2O), and three
different other color reinforcement materials (vinegar, salt, and sodium
carbonate). The mixed fungi were grown on mineral salt glucose medium in
stationary cultures in dark condition for 4 weeks. By using RHS color chart, the
range of colors developed on dyed materials were measured. The results
showed that dyeing repetition and color reinforcement material had a great
effect on the cotton cloth color dyed with the fungal dyes.
Keywords : color reinforcing material, cotton cloth, dyeing repetition, fungal

dyes
183
P2-16
Bacillus subtilis C2 Producing Lipase Isolated from
Bulk Shrimp Paste in Samarinda East Kalimantan
Yati Sudaryati Soeka1 and Sulistiani1

Corresponding author : ceuceu_lipi@yahoo.com
Abstract. Lipase is an enzyme that catalyzes the hydrolysis reaction of lipid

(triacylglycerol) to glycerol and free fatty acids which has been used in various
industrial applications such food, cosmetics, detergents and pharmaceuticals.
Bacillus subtilis C2 isolated from bulk shrimp paste (terasi) in Samarinda East
Kalimantan, it showed producing lipase, able to degrade fat. Lipase activity
was determined with titrimetrically. The ability of strain to degrade fatty
substrates was investigated in the medium 0.1% of olive oil, Virgin Coconut Oil
(VCO), cooking oil, tween 80, and Schleichera oleosa oil. The activities of lipase
treated based the period of incubation, temperature, and pH. The results
showed that the optimum activity of lipase was 1.09 U/mL after five days
incubation, 1.43 μmol/mL at 30°C and 1.96 U/mL at pH 9.0. The enzyme gave
the highest lipase activity 1.76 μmol/mL. used olive oil as a substrate. Also
analyzed the physiology of the bacterium Bacillus subtilis C2-Lipolytic.
Keywords : Bacillus subtilis, lipase, bulk shrimp paste (terasi)
184
P2-17
Biocontrol of postharvest Colletotrichum decay of red
chilies and tomatoes with Bacillus subtilis ATCC
21556
Yuliar

*Corresponding author: yrivaie@yahoo.com
Abstract. Bacillus subtilis ATCC 21556 produce the highest an antifungal of

iturin A compared to others three of B. subtilis strains ATCC 15841, ATCC
27505, and ATCC 21770. An Iturin A has a wide antifungal spectrum activity
therefore making it an ideal potential for controlling of the fungal diseases of
crop not only for preharvest but also postharvest control. Many of fungi cause
of postharvest disease in various fruits and vegetables. Biological control of
postharvest diseases has emerges as an effective alternative control since an
impact of chemical control of post-harvest disease cause serious
consequences for human health and environment. Twenty microliters of B.
subtilis ATCC 21556 (approximately 1 x 107 cell mL-1) was injected into fruit of
red chillies and tomatoes to control its postharvest diseases caused by fungal
of Colletotrichum scovillei 244830. Lesion diameter of C. scovillei 244830
significantly (p<0.005) reduced about 52-64% in chili and 39-44% in tomato.
An invitro test showed that percent growth inhibition of B. subtilis ATCC 21556
against C. scovillei 244830 was about 52.38% at 14 days of the incubation
time. For successful infection of the fungal pathogens into the host plants,
they produce the extracellular enzymes. Qualitative test of the extracellular
enzymes showed that C. scovillei 244830 produced the enzymes of amylase,
lipase, pectate lyase, and protease and it did not produce polygalacturonase.
Keywords : Bacillus subtilis ATCC 21556 ; biocontrol of postharvest; chili,

Colletotrichum; tomato
185
P2-18
Effects of Fermented Feed Supplementation in
Circulated Aquaponic System with catfish (Clarias
sp.) on Growth of Lettuce (Lactuca sativa L.)
Rini Handayani1 & Achmad Dinoto1
1Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia. Cibinong Science

Center Jl. Raya Jakarta-Bogor Km. 46, Cibinong, Jawa Barat 16911 Indonesia
*Corresponding author: handayanirini@yahoo.co.uk
Abstract. Aquaponic systems of aquatic animal-plant are a model for

conversion of waste into plant substrates and its efficacy is estimated
depending on the type of feed given. This study amis to investigate the effect
of fermented feed in the circulating aquaponics system with catfish (Clarias
sp.) on the growth of lettuce (Lactuca sativa L.). The study was carried out in a
circulated serial aquaculture tanks containing catfish. Various inputs of
fermented fish feed was applied including low concentration (6% of fish
weight) and high concentration (9% of fish weight). The growth of lettuce, air
quality, and total microorganisms were measured. As results, maximum
length and width of lettuce were higher in supplementation of higher feed
concentrations (9%), e, as well as tendency height and weight of plant clumps
plant.
Keyword : fermented feed, aquaponic, catfish, lettuce, plant growth
186
P2-19
Collaboration test of rhizobacteria as biostimulant,
vesicular arbuscular mycorhizza (VAM) and graded
dose of NPK fertilizer on the growth of bok choy
(Brassica rapa L.)
Sri Widawati1* and Suliasih1
1Micobiology Division, Research Center for Biology, Indonesian Institute of Sciences,

Cibinong Science Center, Jl. Raya Jakarta-Bogor km 46, Cibinong 16911, Indonesia
*Corresponding author: widadomon@yahoo.com (All authors contributed equally to

this work)
Abstract. Collaboration test of rhizobacteria as biostimulant, vesicular

arbuscular mycorhizza (VAM) and graded dose of NPK fertilizer was a
preliminary experiment to re-fertilize marginal soils. The objective of this
experiment was to obtain the suitable collaboration of rhizobacteria as
biostimulant, VAM and NPK dose that effectively supports the productivity of
the growth media (zeolite) and the growth of bok choy plants. The
experiments design was RCD factorial with 2 treatments which were NPK dose
(0 %, 25 %, 50 %, 75 %, and 100 %) and microbial biostimulant. Rhizobacteria
acting as biostimulant used were nitrogen fixing bacteria or NFB (Azospirillum
sp., Azotobacter sp., Rhizobium radiobacter InaCCB835), phosphate
solubilization bacteria or PSB (Serratia sp., Klebsiella variicola InaCC B827,
Mangrovibacter plantisponsor InaCC B841, Tolumonas osonensis InaCCB831)
and VAM. Each treatment, including control, was repeated 3 times. Bok choy
seeds from each treatment were planted individually on zeolite media and
grown in a greenhouse for 40 days. The results showed that the treatment of
MIXN1 (NFB + PSB + VAM + NPK 25%) and MIXN2 (NFB + PSB + VAM + NPK
50%) were effective collaborations of biostimulating rhizobacteria, VAM and
NPK dose on supporting the fertility of growth media with the number of
rhizobacteria of 107 cfu g-1 zeolite and supporting optimization of bok choy
plant growth and the percentage of bok choy root colonization by VAM with a
very high level of infection.
Keywords : Rhizobacteria, Biostimulant, VAM, NPK, Bok choy
187
P2-20
Screening of Some Plant Extracts from Lake Toba-
North Sumatra for controlling Wood-Rotting fungi
Deni Zulfiana*, Maya Ismayati, Dita Meisyara, Anis Sri Lestari, Anugerah Fajar, Apriwi
Zulfitri, Khoirul Himmi Setiawan, and Didi Tarmadi

*Corresponding author: zulfiana@biomaterial.lipi.go.id
Abstract. The wood-rot fungus is one of the most destructive organisms for
wood, wood products and building structures besides termites and beetles.
Until today, wood-root fungi are still difficult to control, so it is necessary to
search for new natural ingredients to be developed as wood preservatives.
The purpose of this study was to screen some plant extracts from the area
surrounding Lake Toba as anti-wood-rot fungi. This study evaluated antifungal
properties of 6 different plant extracts dissolved in methanol against 9 species
of wood-rot fungi. The activity was determined using the method of Mohareb
et al., (2013) by growing the fungi on PDA supplemented with the plant
extracts. Parameters observed included diameter of fungal growth and
inhibition percentage of fungal growth by plant extracts. Extracts of Haurese
leaves, Pirdot leaves and Sihunting-hunting leaves displayed remarkable
growth inhibition activity ,up to 100%, of the wood rot fungi therefore are
potentially be studied further as anti wood-rot agent.
Keywords: brown-rot fungi, Lake Toba, plant extracts, white-rot fungi
188
P2-21
Bioremediation of Phenanthrene by Ocean Bacteria
from the Collection of Microbiology Laboratory in
LIPI Cibinong, Bogor
Nabila Ayu Budianti1*
1
Department of Biology,
FMIPA Universitas Indonesia,
Universitas Indonesia, Depok,
Jawa Barat 16424, Indonesia
*Corresponding author: nabila.ayu61@ui.ac.id
Abstract. Phenanthrene is a polycyclic aromatic hydrocarbons (PAH) that has

3 ring, appear as a colourless, sometimes in white or pale yellow-green solids
which can be found in polluted air, water and soil. This PAH model is
categorized as a priority environmental pollutants by the United States
Environmental Protection Agency ( US EPA). Recently, some treatment
method have been performed, such as incineration, dechlorination, and also
UV-oxidation. But this method cause several problems, including higher rate
of cost, and more complex regulatory burden. Therefor, a solution is needed
to address this problems without causing any more damage. Bioremediation
is an environmentally friendly treatment to fight pollution. This research
intended to find a potential bacteria from the LIPI – Microbiology Laboratory
collection that have the ability to degrade phenanthrene and shows the
correlation of the bacteria with phenanthrene concentration.
Keywords : bioremediation, degradation, ocean, phenanthrene, PAH
189
EARTH
SCIENCE
190
P3-22
Himawari-8 Rainfall Estimation from Infrared
Channels Based on Machine Learning Methods
Risyanto1*, Teguh Harjana2, Farid Lasmono2
1,2Center for Atmospheric Science and Technology, Indonesia National Space and
Aeronautics Agency
Jln. Dr. Djunjunan 133, Bandung 40173, Indonesia
*Corresponding author: risyanto@lapan.go.id
Abstract. An accurate information of rainfall estimate from remotely sensed

instrument is highly needed for many applications, including the disaster early
warning system that requires heavy rainfall prediction for mitigation purpose.
In this study we implemented machine learning methods to estimate and
predict rainfall from high temporal resolution data of Himawari-8 over a large
area of Indonesia, where floods and landslides caused by heavy rainfall events
have been the most frequent disaster for the last 10 years. All brightness
temperature of Himawari-8 infrareds (IR) channels were involved to estimate
rainfall at current time (nowcasting) and to predict at one and two hours later
by using 9 different machine learning models. The rainfall data product from
Global Satellite Mapping of Precipitation (GSMaP) were used for the data
training. The results showed that the linear discriminant analysis (LDA) model
has been the most accurate compared to the others. A good accuracy from
the LDA has been obtained for nowcasting case (accuracy = 0.81) as well as for
prediction at one and two hours later (accuracy = 0.79, and 0.78 respectively).
A comparison test has also been performed to determine which variables have
the most significant contribution to the rainfall retrieved. It showed that 3 IR
channels at 6.2, 10.4, and 13.3 µm were the minimum predictors that should
be used for the good rainfall estimation result. An addition of other predictors
such as other channels of IR or a combination of brightness temperature
difference (BTD) could increase its accuracy, i.e. 0.01-0.02 which is not too
significant.
Keywords : Himawari-8, infrareds, machine learning, nowcasting, rainfall
191
P3-23
A Comparative Analysis of the Wind and Wave on the
Extreme Weather Events in the Southern Sea of Java
L.Q. Avia1*
1Center
for Atmospheric Science and Technology, LAPAN, Jl. Dr. Djundjunan No. 133,
Bandung 40173, Indonesia
*E-mail: qodrita@yahoo.com
Abstract. The tropical cyclones (TC) are a serious danger to marine and coastal
areas in the tropics because they cause strong winds and extreme sea wave
height. The combination of these two parameters will be dangerous for
shipping safety. The purpose of this study is to investigate the effect of TC
Cempaka (21-29 November 2017) and TC Dahlia (26 November-4 December
2017) on significant wave heights and surface winds. The relationship
between two parameters is also discussed in this paper. Both of these tropical
cyclone events are interesting to study because they occur in the southern sea
of Java and in adjacent time periods. Special attention is paid to the southern
sea of Java because it has high shipping traffic where 8 locations are used as
case studies. Significant wave height and wind conditions are evaluated using
data obtained from the European Mid-Term Weather Forecast Center
(ECMWF) with 6 hourly temporal resolution (time UTC 00.00, 06.00, 12.00,
18.00) and 0.5 x 0.5 degrees spatial resolution of. The obtained results show
that the influence of TC Dahlia on significant wave height and surface wind
appears stronger than the influence of TC Cempaka. During TC Dahlia, the
strongest relationship between wind and significant wave height was in the
southern sea of West Java 01 and West Java 02 with correlation coefficients
of 0.83 and 0.89, respectively. While the frequencies of extreme wave heights
at these locations were 38.89% and 44.44%, respectively.
Keywords : extreme weather, southern sea of Java, tropical cyclone, wave,

wind
192
P3-24
Impact of Radar Data Assimilation on the Accuracy of
Heavy Rain Events Prediction in Bangka Island (Case
Study: Flood 8-9 February 2016)
Nur Setiawan1,2*, Nurjanna Joko Trilaksono1, and Indra Gustari2
1Facultyof Earth Science and Technology, Bandung Institute of Technology,

Bandung, Indonesia
2Indonesian Agency for Meteorological, Climatological and Geophysics, Indonesia
*Corresponding author : nur.setiawan77@gmail.com
Abstract. Numerical weather prediction (NWP) is a prediction method that

now become the world standard weather predictions. However, the use of
NWP does not necessarily increase the accuracy of predictions. Data
Assimilation using radar is one of improvements effort to initial condition
considered to be able improve the accuracy of weather prediction. The
purpose of this study is to determine the impact of radar data assimilation on
Weather Research Forecasting (WRF) model in predicting the phenomenon of
heavy rain on Bangka Island. In this study we employ the numerical model
WRF to perform experiment model without assimilation and with radar data
assimilation using WRFDA (WRF data assimilation) model with 3DVar systems.
The model outputs consist of refelektivity and rainfall distribution value are
verified with qualitative observational data. Quantitative verification is also
done by testing the skill and accuracy of the model against observations. The
results obtained show that WRF with radar data assimilation provides better
spatial and points simulation results and improved prediction results
compared to WRF without assimilation.
Keyword : NWP, Data Assimilation, WRF, WRFDA, Radar
193
P3-25
Evaluation of planetary boundary layer schemes on
Weather Research Forecasting by using intensive
observation at Kototabang
Ibnu Fathrio1, Farid Lasmono1, Risyanto1 and Ina Juaeni1
1Pusat Sains dan Teknologi Atmosfer, LAPAN

Jl. dr Junjunan no 133 Bandung, West Java 40173
*Corresponding author: ibnu.fathrio@lapan.go.id
Abstract. Planetary boundary layer (PBL) scheme is responsible for the

transport of energy, heat, momentum and moisture from lower boundary to
upper atmosphere layers. Our experiment using six different schemes of PBL
available on weather research forecasting (WRF) aims to evaluate the
performance of PBL scheme in representing the vertical profile of the
atmosphere over Kototabang. Intensive observation during boreal spring in
2002 using radiosonde and Equatorial Atmosphere Radar provided were used
to verify the results of WRF. Our results showed the actual PBL height at noon
is also underestimated up to 500 meters difference. PBL scheme with warmer
(colder) surface temperature and less (greater) PBL-moisture tend to
reproduce higher (lower) PBL height. ACM2 scheme displays highest PBL
height, while MYNN2.5 and MYJ schemes display lower PBL height during the
day. Our important findings showed that all schemes display cold and wet bias
below ~1 km height which happens during the daytime.
Keywords : Planetary Boundary Layer, PBL scheme, WRF, Kototabang
194
P3-26
Spatial Analysis of Correlation between Predicted
Rainfall Based on Dynamic Model and Satellite
Satyawardhana, H.1* and E. Yulihastin1
1Research Centre of Atmospheric Science and Technology, National Institute of

Aeronautics and Space
Bandung 40173, Indonesia
*Corresponding author: hariessatha@gmail.com
Abstract. Indonesia is located in the equator and flanked by 2 continents

(Australia and Asia) has two seasons (rain and dry) which are very striking
difference. Season prediction is needed to support the main economic sectors
in Indonesia, especially the agricultural sector. This study uses a dynamic
atmosphere model, the Conformal Cubic Atmospheric Model (CCAM). Season
prediction in this study uses initial and boundary conditions from The Global
Forecast System (GFS) data and 11 predictive SST data from The Predictive
Ocean Atmosphere Model for Australia (POAMA) with the Indonesian domain.
The results of this season prediction research are predicted rainfall for the next
8 months with a spatial resolution of 32 km and daily temporal. Comparative
data used for validation are rainfall data from the GSMaP satellite. The results
of this study are areas that have a high correlation found in southern
Indonesia. This occurs in both simulations, simulations in the rainy and dry
seasons.
Keywords : Season prediction. Model, rainfall
195
P3-27
The Analysis of tropical cyclones that occurred in the
southern sea of Java during the period 2004-2019
and their effects on sea-atmospheric conditions
I Sofiati1* and M F Putranto2
1,2Center for Atmospheric Science and Technology, LAPAN,

Jl. Dr. Djundjunan No. 133, Bandung 40173, Indonesia
*Corresponding author: sofiati07@gmail.com
Abstract. Tropical cyclones are an important part of the atmospheric

circulation system, which transfers heat from the equatorial region to higher
latitudes. The birth of a tropical cyclone will have an impact on increasing the
intensity of heavy rain, wave height, strong winds, and the potential for
lightning. The purpose of this study is to analyse tropical cyclones and their
impact on sea and atmospheric conditions that occur in the southern of Java.
The data used in this study is hourly rainfall data, Madden Julian Oscillation
(MJO), wind (direction and speed), significant wave height, sea surface
temperature, and sea level pressure from the European Centre for Medium-
Range Weather Forecasts (ECMWF). The method used in this study is
averaging hourly data into daily data for each sea-atmospheric parameter
during the 16-year observation period (2003-2019), and representing it in the
form of descriptive daily on before, being and after the occurrence of tropical
cyclones. From the results of the analysis it can be concluded that there were
17 tropical cyclones which mostly occurred in April, resulting in a significant
increase in intensity of sea-atmospheric parameters (rainfall, significant wave
height, and wind direction & speed), but decreased for sea level pressure. As
from the Riley tropical cyclone event that occurred on (24-26) January 2019,
rainfall increased in intensity to 951 mm/ day, significant wave heights
reaching more than 4.4 m with wind speeds of more than 13 m/ sec, sea level
pressure is less than 995 hPa, and the average MJO condition is in phase 2, 3,
4, or 5, with a strong period.
Keywords : Tropical cyclone, Madden Julian Oscillation (MJO), wind (direction

and speed), significant wave height.
196
P3-28
Variability and sensitivity of stability indices at the
tropical region
Ina Juaeni1*, Risyanto1, Ibnu Fathrio1, Teguh Harjana1 and Trismidianto1
1ResearchCenter for Atmospheric Science and Technology,

Bandung, Indonesia
*Corresponding author: inajuaeni@yahoo.com
Abstract. Stability index data obtained from the Radiosonde launch at the
Space and Atmospheric Observation Station Agam District (West Sumatera-
Indonesia) in 30 August to 2 September 2016 (three times launches a day), 5
March to 9 March 2018 (twice launches a day ) and 29 April to 2 May 2019
(once launch a day) used Vaisala RS41-SG. The purpose of this study is to
investigate the stability indices variations and finding the best indicators for
convective disturbances at the tropical region. The stability indices studied are
CINH, CAPE, K-index, Ko-index, S-index, SI, LI, TT-index, and the values of
temperature, pressure, and height of the stability layers (LCL, LFC, CCL, and EL
K-index, S-index, and TT-index indicate that atmospheric conditions are always
unstable. The indices fluctuate slightly as well as temperatures and pressures
at stability level. While CAPE, Ko-Index, LI, SI, CINH, temperature and pressure
of EL, and the elevations of LCL, LFC, CCL, El are very fluctuating and show
clearly the stable and unstable conditions. The last indices can be treated as
an indicator for convective disturbances due to their sensitivity for the
changes. Three different observation periods produce three sets of characters
for each index.
Keywords : index, radiosonde, stability, sensitivity, tropic, variability
197
Community-based
development and
socioeconomic
science
198
P4-29
Relationship Between The Socioeconomic
Characteristics Of Farmers And The Competency
Level Of Rice Seed Producers In Bogor Regency
Linar Humaira1* Hadi Cosra2
1, 2Agribusiness
Study Program, Faculty of Agriculture, Nusa Bangsa University, Tanah
Sareal-Bogor 16166, West Java Province, Indonesia
*Corresponding author: linar.humaira@yahoo.com
Abstract. The purpose of this study is to analyze the characteristics and

competence level of rice seed producers in Bogor regency of West Java. This
research use survey method to 59 rice seed producer which spread in the
three sub districts Bogor district. The study was conducted from April to May
2017. Data analysis using descriptive statistical analysis and statistical analysis
of rank spearman inferences. The result showed that the characteristics of
farmers produers of rice deed on average productive age, low education,
experienced, narrow, lots of family dependents, low production, low income,
hugh motivation, contact with hugh councelors and high capital. The
competency level of the management aspects category high enough, while
competence of technical aspects 71,4% have category low. Factors that have
a relationship with the competence of rice seed producers is length of farming,
land area, motivation, production, income, education and access to capital. To
improve the competence of rice farmers is needed to improve the quality of
characteristics and improvement of the technical aspects competence by
providing additional non formal education in the form of trainings related to
rice seed.
Keywords : competence, produsers, rice seed
199
P4-30
Estimating Ecosystem Services of Urban Green Space
in a Tourism Landscape of Bali, Indonesia
Laily Mukaromah
Indonesian Institute of Sciences

Jl. Raya Surabaya Malang km 65 Purwodadi Pasuruan
*Corresponding author: laily.mukarromah@gmail.com
Abstract. Urban Ecosystem Service is essential for health and quality of life for
human beings. In this study, we evaluate the changes in land cover and Gross
Primary Productivity (GPP) as proxies of Urban Ecosystem Service in Sanur
Region, Bali, Indonesia. Land cover and Gross Primary Productivity (GPP) were
calculated using satellite data ALOS/Avnir-2 and Sentinel-2A from 2006 to
2015. Results showed an increasing trend GPP with annual average value
144.73 gC/m2/yr while land use changed from green spaces into
multifunctional buildings. This study highlights the high potential of
ALOS/Avnir-2 and Sentinel-2A for GPP estimation as a function of atmospheric
carbon extraction rate of terrestrial ecosystems.
200
P4-31
Interaction Quality among Doctor and Hypertension
Patiens in Primary Health Care, in Medan, Indonesia
Wahyuni, AS1., Amelia, R1 Rafiqi, A2., Nainggolan, AR2., Sinaga, AFP2.,
1Department of Community Medicine, Faculty of Medicine, Universitas Sumatera

Utara, Indonesia
2Faculty of Medicine, Universitas Sumatera Utara, Indonesia
Correspondent author: arlinda@usu.ac.id
Abstract. Communication is the process of delivering and receiving messages

from someone who is shared with others. Communicating means helping to
convey a message to later be known and understood together. Message in
communication is used in choosing and making decisions. Communication
between doctor and patient involves deep interaction between the two
parties. The treatment process is performed by doctors to their patients based
on a relationship of mutual trust with each other. The aim of this study is find
out the quality of interaction of hypertension patients at puskesmas Amplas,
Medan, Indonesia. Descriptive research methods with cross sectional design
using an interaction questionnaire. Results. Study openness of respondents to
the interpersonal communication of health workers in carrying out their
obligationt is 36 respondents (50,0%), empathy by health workers to the
families of patients as many as 42 people (53,8%), the attitude of supporting
respondents obtained result as many as 44 people (56,4%), the positive
attitude of the workforce is 36 respondents (50,0%), the equality of
respondents is 41 respondents (52,6%). From the 5 questions above it can be
concluded that the quality of interaction of hipertensive patients in puskesmas
Amplas is in the good category.
Keywords: Communication, Interaction, doctor, patient, Hypertension
201
APPLIED SCIENCE
AND TECHNOLOGY
202
P5-32
Effect of Silica in Sembilang Bamboo Skin on the
Adhesion of Citric Acid and Bamboo Particles
Firda Aulya Syamani1*, Agus Zultri Arifqi2, Yusuf Sudo Hadi2, Sudarmanto1, Fazhar
Akbar1, Sukma Surya Kusumah1, Kurnia Wiji Prasetiyo1, Lilik Astari1, Subyakto1 and
Kenji Umemura3

2Faculty of Forestry, IPB University,
Jl. Lingkar Kampus IPB Darmaga, Bogor 16680, Indonesia

3Research Institute for Sustainable Humanosphere, Kyoto University
*Corresponding author: firda.syamani@biomaterial.lipi.go.id
Abstract. Some components in particleboards raw materials could be act as

barrier in adhesion of particles to produce high quality particleboards.
Bamboo skin contain silica that was suspected as obstruction component in
particle adhesion during hot pressing in particle boards production. The aim
of the study was to evaluate physical, mechanical, wettability characteristics
of sembilang bamboo particleboards using citric acid as adhesive. We compare
the particleboards characteristics of sembilang bamboo produced by regular
particles to unskinned particles with varied adhesive content (citric acid: 15%,
20%, 25%). The single layered particleboards were produced with density of
0.8 g/cm3 and the particleboards testing refers to JIS A5908:2003. Based on
the results of the research, particleboards made from unskinned bamboo
particles, bonded with 25% citric acid perform the best particleboards physical
properties (water absorption = 19.38% and thickness swelling = 5.18%).
Particleboards with the highest modulus of elasticity (4135 MPa) was
produced from regular bamboo particles bonded with 15% citric acid. While
particle boards made from unskinned bamboo particles bonded with 20%
citric acid show the highest modulus of rupture (20.69 MPa). All the
particleboards in this study were met JIS A5908 on internal bond for
particleboards type 18 (IB min 0.3 MPa). Furthermore, particleboards made
from unskinned bamboo particle show higher internal bond characteristics
compare to particleboards made from reguler bamboo particles. At same level
of citric acid, the screw withdrawal properties of bamboo particleboards made
203
from reguler particles were not significantly difference wtih particleboards

made from unskinned particles. The wettability of unskinned bamboo particles
were higher compare to reguler bamboo particles which resulted in higher
physical and mechanical properties.
Keywords : citric acid, particleboards, physical and mechanical characteristics,

unskinned sembilang bamboo particles, wettability
204
P5-33
Obtaining Different Charged Cellulose Nanofibrils by
TEMPO-mediated Oxidation
Nanang Masruchin1, Putri Amanda1, Wida Banar Kusumaningrum1, Lisman
Suryanegara1 and Arif Nuryawan2,*

2Department of Forest Products Technology, Faculty of Forestry, Universitas Sumatera
Utara, Jl. Tri Dharma Ujung No.1 Kampus USU Medan, North Sumatera Indonesia
20155
*Corresponding author: arif5@usu.ac.id
Abstract. Cellulose nanofibrils (CNF) were obtained by TEMPO (2,2,6,6-

tetramethylpiperidine-1-oxyl radical)-mediated oxidation from hardwood
bleached kraft pulp. Different charged of CNF were tuned by adding different
amount of sodium hypochlorite (NaClO) as oxidator at 3, 4, 7, 10 and 15 mL.
The amount of carboxyl groups and aldehyde groups were quantitatively
measured using conductometric titration. Results showed that the size
distribution (in nanometer range) of CNF increased as the oxidant amount
increased, however it decreased after 10 mL addition indicated the
breakdown of cellulose degree of polymerization. An optimum oxidant
amount facilitated thinner and longer CNF. Zeta potential, microscopy image
and thermogravimetry analysis of obtained CNF were also discussed in this
report. Here, we reported that different charged of CNF will further
correspond to its applications
Keywords : carboxyl groups, cellulose nanofibrils, TEMPO-mediated oxidation,

zeta potential
205
P5-34
The Utilization of Oil Palm Leaves (Elaeis guineensis
Jacq.) Waste as an Antibacterial Solid Bar Soap
Amelia Febriani1*, Vilya Syafriana1, Hendra Afriyando1, dan Yayah Siti Djuhariah1
1Facultyof Pharmacy, National Institute of Science and Technology, Jl. Moh. Kahfi II,
Srengseng Sawah, Jagakarsa, Jakarta 12640
*Corresponding author: ameliafebriani@istn.ac.id
Abstract. Oil palm leaves (Elaeis guineensis Jacq), which have been
underutilized by the community and become waste that usually stacked
around the trees, have a potential to be used as active ingredients for making
antibacterial solid bar soap. The chemical content of oil palm leaves are
tannins, alkaloids and flavonoids that known had antibacterial activity. This
research aims to produce oil palm leaves extracts into an active ingredient of
solid bar soap formulation with antibacterial activity against Escherichia coli
and Staphylococcus aureus and also to evaluate the quality of the solid bar
soap. The soap was formulated into 3 formulas with varying concentrations of
oil palm leaves ethanolic extract that was F1 (1% ), F2 (2%), and F3 (4%). Oil
palm leaves extract was prepared with maceration using ethanol 70%.
Antibacterial activity assay of the solid bar soap was carried out using a disc
diffusion method with tetracycline as the positive control (K+) and distilled
water as the negative control (K-). The soap quality was evaluated for its
organoleptic, foam level and foam stability, pH, hardness, water content and
free fatty acid. The results showed that all three solid soap formulas meet the
soap quality requirements of SNI No. 3532-2016. The solid bar soap did not
have inhibition properties against Escherichia coli. However, had inhibition
effect against Staphylococcus aureus with an average Inhibition Zone
Diameter (IZD) on soap base, F1, F2, and F3 was 8.02 mm, 8.53 mm, 10..53
mm, 12.91 mm respectively.
Keywords: antibacterial activity, Elaeis guineensis Jacq., ethanol extract, oil

palm leaves waste, solid soap
206
P5-35
Phylogenetic Study of Some Ganoderma from
Indonesia: a preliminary study
Dewi Susan1*, Krisyanti Budipramana2,3, Komar R Wirasutisna2, Sukrasno2,4
aHerbarium Bogoriense, Botany Division, Research Center for Biology, Indonesian
Institute of Sciences (LIPI)
bSchool of Pharmacy, Institut Teknologi Bandung (ITB)
cDepartment of Pharmaceutical Biology, Faculty of Pharmacy, University of Surabaya,
Surabaya 60293, Indonesia

dPharmacy Program, Department of Science, Sumatera Institute of Technology, South
Lampung, Indonesia
*Corresponding author: dewi013@lipi.go.id
Abstract. Ganoderma is a genus from order Polyporales with

almost 400 species worldwide. This genus has long been known as
traditional medicine in Chinese, Japan and Korea, but in the other
hand this member of genus also known caused major diseases for
some commodity crops plantations. Taxonomically, this genus is
one of the most difficult to identify because of many similar
morphological characters. In order to overcome this problem,
molecular identification was used to reveal the species of
Ganoderma in Indonesia. For this preliminary study, some samples
were collected from several places in Indonesia. Four species
namely Ganoderma australe, G. orbiforme, G. philippi and G.
steyaertanum was identified in this study.
Keywords: Ganodermataceae, Phylogeny, Taxonomy
207

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The 9th International Symposium for Sustainable Humanosphere (ISSH)

Bogor, 28 -29 October 2019

 Dita Meisyara, S.Si.

Welcome to HSS-ISSH 2019

Recently, forest science, bioscience, earth science, community-based

Organizing Committee Chair

(GSA, Kyoto University, Japan)

13.00 – 13.10 Brief Introduction of e-Asia Project

Monday, September 28th 2019 Session I: 15.55 – 16.55

Name Tittle Institution Page

Tri Effect of Artocarpus heterophyllus USU6 108

Name Tittle Institution Page

Particleboards of Tea Leaf waste

Tuesday, September 29th 2019 Session II: 14.40 – 15.40

Name Tittle Institution Page

Attitude Of The Mothers Who Have A

Name Tittle Institution Page

Kartini The Relatioship of Dimension and IPB10 102

Session III: 15.55 – 16.55

Chici Performance Test Of Adsorption Of UGM8 155

Name Tittle Institution Page

Riksfardini Development of Bamboo LIPI5 121

Name Tittle Institution Page

Name Tittle Institution Page

Ikhsan The influences of social-community LIPI5 161

Code Name Title Page

various plants on soybean (Glycine

P2 – 19 Sri Widawati Collaboration test of rhizobacteria as 187

P3 – 23 Lely Qodrita A Comparative Analysis of the Wind 192

and their effects on sea-

Dr. Arif Satria, SP, M.Si

Rector of IPB University Email : arifsatria@apps.ipb.ac.id

 Doctoral Degree in Marine Policy, Kagoshima University, Japan

1996 – 1997 Secretary of Rector IPB

Dr. Bima Arya

 Doctoral Degree in Political Science, Australian National University,

1998 – 2001 Lecturer at Parahyangan University

2019 Yokoyama, T., H. Jin, H. Shinagawa, and H. Liu, Seeding of

Equatorial Ionospheric Disturbances and Its Social Impacts

The Earth’s upper atmosphere above 100 km altitude is partially

three-dimensional (3D) simulations became popular tools for further

Key words: equatorial ionosphere, space weather, plasma bubble,

1978-1981 Doctor of Science, Graduate School of Agriculture, Hokkaido

1976-1978 Master of Science, Graduate School of Agriculture, Hokkaido

1972-1976 Bachelor of Science, Faculty of Agriculture, Hokkaido

Lahiru S. Wijedasa, Susan E. Page, Christopher D. Evans, Mitsuru

Eds. by Mitsuru Osaki and Nobuyuki Tsuji: "Tropical Peatland

Lead Authors: Gusti Zakaria Anshari (Indonesia), Kenneth Byrne

An Innovated Tropical Peatland Management

Long term research of tropical peatland by Hokkaido University have

In conclusion, nutrients and oxygen should be applied from peatland

Key words: AeroHydro Culture, Ground Water Table, Land Surface

1997 / 2001 - Head of Chemistry Department

2012 member of lab of Excellence ARBRE

Key-word: Biodegradation - Biocides -Chemical modification - Extractives -

Université de Lorraine, Inra, EA 4370 USC 1445 LERMAB, Faculté des

Key words : chemical modification, durability, biocide, thermal modification,

Mohd Hazwan Hussin

School of Chemical Sciences Office Tel : +604 653 6378

Sherwyn Hamidon, Teoh Zhi Qiang, M. Hazwan Hussin), Materials

kinetic studies, (Zaharaddeen N. Garba, M. Hazwan Hussin, Ahmad

3) Condensed tannins from mangrove and grape pomace as renewable

Syima Sharifuddin, Muhammad Azroie Mohamed Yusoff), Science