Organelle Atlas: Appendix To Chapter 4

Organelle Atlas
APPENDIX TO CHAPTER 4
This atlas contains images of cellular organelles visualized using a wide variety of
reagents and techniques and demonstrates the diversity of methods for exploring the cell.
The atlas consists of the figures listed in Table 4A.1.
Table 4A.1 Figures in Organelle Atlasa
Figure Organelle(s)/structure(s) Visualized using:

Fig. 4A.1 Mitotic spindle and chromosomes γ-tubulin-GFP, anti-β-tubulin antibody,
Hoechst 33342
Fig. 4A.2 DNA and β-tubulin Hoechst 33342, anti-β-tubulin antibody
Fig. 4A.3 Nuclear pore complexes POM121-GFP
Fig. 4A.4 Nuclear envelope Lamin B receptor–GFP
Fig. 4A.5 Myosin, actin stress fibers Anti-myosin antibody, rhodamine-
conjugated phalloidin
Fig. 4A.6 Focal adhesions Anti-vinculin antibody
Fig. 4A.7 Intermediate filaments Anti-vimentin antibody
Fig. 4A.8 Microtubules and Golgi complex Anti-tubulin and anti-
galactosyltransferase antibodies
Fig. 4A.9 Endoplasmic reticulum DiOC6
Fig. 4A.10 Endoplasmic reticulum Anti-ER-resident protein antibodies
Fig. 4A.11 Lysosomes Rhodamine-ovalbumin
Fig. 4A.12 Lysosomes Anti-LEP100 antibodies
Fig. 4A.13 Lysosomes, endosomes Rhodamine-conjugated wheat germ
agglutinin
Fig. 4A.14 Golgi complex Anti–mannosidase II antibodies,
brefeldin A, nocodazole
Fig. 4A.15 Mitochondria TMRE, calcium green AM
Fig. 4A.16 Endosomes Anti-transferrin antibodies
Fig. 4A.17 Trans-Golgi network Anti-TGN38 antibodies
Fig. 4A.18 Trans-Golgi network, endosomes Monoclonal anti-AP1 antibodies (100/3)
Fig. 4A.19 Trans-Golgi network, endosomes Anti-TGN46 and anti-AP3 antibodies
Fig. 4A.20 Plasma membrane clathrin-coated Anti-AP2 antibodies
pits, clathrin-coated vesicles
Fig. 4A.21 Plasma membrane clathrin-coated Monoclonal anti-clathrin antibodies
pits, trans-Golgi network, endo- (X22)
somes, clathrin-coated vesicles
Fig. 4A.22 Fibronectin (an extracellular matrix Rhodamine-conjugated anti-fibronectin
protein), nuclei antibody, DAPI
aAbbreviations: DAPI, 4,6-diamidino-2-phenylindole; DiOC , dicarbocyanine dye; GFP, green fluorescent protein; TGN,
6
trans-Golgi network; TMRE, tetramethylrhodamine ethylester.
Organelle Atlas
Current Protocols in Cell Biology (1998) 4A.1-4A.12 4A.1

Copyright © 1998 by John Wiley & Sons, Inc.
Figure 4A.1 Mitotic NRK cell in metaphase. Red, mouse anti-β-tubulin antibody;
green, γ-tubulin-GFP; blue, Hoechst 33342. Figure provided by Jan Ellenberg,
NICHD/NIH.
Figure 4A.2 Distribution of DNA (Hoechst 33342, blue) and β-

tubulin (anti-β-tubulin antibody, red) in interphase NRK cells. Figure
provided by Jan Ellenberg, NICHD/NIH.
Organelle Atlas
4A.2
Current Protocols in Cell Biology
Figure 4A.3 Nuclear pore complexes in PTK2 cell stained with POM121 tagged
with green fluorescent protein (GFP). Figure provided by Jan Ellenberg, NICHD/NIH.
Organelle Atlas
4A.3
Figure 4A.4 Nuclear envelope in COS cell labeled with lamin B receptor tagged
with green fluorescent protein (GFP). Figure provided by Jan Ellenberg, NICHD/NIH.
Organelle Atlas
4A.4
Figure 4A.5 (A) Myosin localization in formaldehyde-fixed NRK cell labeled with mouse anti-
myosin antibodies. (B) Stress fibers in the same formaldehyde-fixed NRK cell labeled with
rhodamine-conjugated phalloidin. Figure provided by Julie Donaldson, NHBLI/NIH.
Figure 4A.6 Focal adhesions in formaldehyde-fixed

NRK cell labeled with mouse anti-vinculin antibodies.
Figure provided by Julie Donaldson, NHBLI/NIH.
Figure 4A.8 Microtubules and Golgi complex in HeLa cell

stained with anti-tubulin antibodies (green) and antibodies to
the Golgi complex enzyme galactosyl transferase (yellow-or-
ange). Figure provided by Jennifer Lippincott-Schwartz,
NICHD/NIH.
Figure 4A.7 Intermediate filaments in formalde-

hyde-fixed NRK cell labeled with rabbit anti-vimentin
antibodies. Figure provided by Julie Donaldson,
NHBLI/NIH.
Organelle Atlas
4A.5
Figure 4A.9 Endoplasmic reticulum (ER) in frog skin cell labeled with DiOC6. Figure provided by Mark
Terasaki, University of Connecticut Health Center.
Organelle Atlas
4A.6
Figure 4A.10 Endoplasmic reticulum (ER) in NRK cell labeled with
antibodies to ER-resident proteins (from D. Louvard). Figure provided
by Jennifer Lippincott-Schwartz, NICHD/NIH.
Figure 4A.11 Lysosomes in chicken fibroblast labeled

with rhodamine-conjugated ovalbumin taken up by the
cells over 4 hr. Figure provided by Jennitfer Lippincott-
Schwartz, NICHD/NIH.
Figure 4A.12 Lysosomes in chicken fi-
broblast labeled with antibodies to LEP100,
a 100-kDa lysosomal membrane protein.
Figure provided by Jennifer Lippincott-
Schwartz, NICHD/NIH.
Organelle Atlas
4A.7
Figure 4A.13 HeLa cell stained with rhodamine-conjugated
wheat germ agglutinin (WGA), which binds to sialic acid and
N-acetylglucosaminyl residues on glycoproteins. Glycoproteins
enriched in these residues are found in lysosomes and en-
dosomes. Figure provided by Jennifer Lippincott-Schwartz,
NICHD/NIH.
Figure 4A.14 Distribution of antibodies to the

Golgi enzyme mannosidase II in fixed and
permeabilized HeLa cells (A) untreated; (B) treated
with brefeldin A; or (C) treated with nocodazole,
which depolymerizes microtubules. Figure provided
by Jennifer Lippincott-Schwartz, NICHD/NIH.
Organelle Atlas
4A.8
Figure 4A.15 Neuroblastoma cell stained with TMRE to label
mitochondria (orange) and with calcium green AM to monitor
calcium. Figure provided by Ion Moran, University of Connecticut
Health Center.
Figure 4A.17 Trans-Golgi network

(TGN) in NRK cell labeled with anti-
bodies to TGN-38, a membrane pro-
tein. Figure provided by Jennifer
Lippincott-Schwartz, NICHD/NIH.
Figure 4A.16 Distribution of endosomes in NRK cell

incubated in the presence of iron-saturated transferrin
and labeled with fluorescent antibodies to transferrin.
Figure provided by Jennifer Lippincott-Schwartz,
NICHD/NIH.
Organelle Atlas
4A.9
Figure 4A.18 Distribution of the adaptor complex AP1 in human fibroblasts stained
with monoclonal 100/3 in the trans-Golgi network and endosomal structures. Figure
provided by Juan Bonifacino, NICHD/NIH.
Figure 4A.20 Localization of adaptor complex

AP2 to plasma membrane clathrin-coated pits
and clathrin-coated vesicles in HeLa cells labeled
Figure 4A.19 Distribution of the trans-Golgi net- with anti-AP2 antibodies. Figure provided by Juan
work and the adaptor complex AP3 in human fi- Bonifacino, NICHD/NIH.
broblasts labeled with antibodies to TGN46 (green)
and AP3 (red). Figure provided by Juan Bonifacino,
NICHD/NIH.
Organelle Atlas
4A.10
Figure 4A.21 Clathrin distribution in human fibroblasts la-
beled with monoclonal antibody X22 (Affinity Bioreagents).
Figure provided by Juan Bonifacino, NICHD/NIH.
Organelle Atlas
4A.11
Figure 4A.22 Distribution of the extracellular matrix protein fibronectin in cells labeled with rhodamine-
conjugated antibody to fibronectin (red) and DAPI (blue). Figure provided by Kenneth Yamada, NIDR/NIH.
Organelle Atlas
4A.12

Organelle Atlas: Appendix To Chapter 4

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Organelle Atlas

Table 4A.1 Figures in Organelle Atlasa

Figure Organelle(s)/structure(s) Visualized using:

Current Protocols in Cell Biology (1998) 4A.1-4A.12 4A.1

Figure 4A.2 Distribution of DNA (Hoechst 33342, blue) and β-

Figure 4A.6 Focal adhesions in formaldehyde-fixed

Figure 4A.8 Microtubules and Golgi complex in HeLa cell

Figure 4A.7 Intermediate filaments in formalde-

Figure 4A.11 Lysosomes in chicken fibroblast labeled

Figure 4A.14 Distribution of antibodies to the

Figure 4A.17 Trans-Golgi network

Figure 4A.16 Distribution of endosomes in NRK cell

Figure 4A.20 Localization of adaptor complex

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